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At least 127 records · Page 7Linked to original sources
PubMed1984

Video digitizer analysis of birefringence along the lengths of single chromosomal spindle fibres. II. Crane-fly spermatocyte chromosomal spindle fibres are not temperature-labile.

Retardations were measured along the lengths of single chromosomal spindle fibres, from metaphase through anaphase, from video-taped images of crane-fly spermatocytes incubated at various temperatures (4-30 degrees C). These measurements were made using a video digitizer interfaced to a microcomputer. Over most of the range of temperatures at which normal anaphase movement occurs the chromosomal spindle fibres are not temperature-labile. The non-specific and continuous fibre birefringence is temperature-labile, however. The data are discussed with respect to the 'dynamic equilibrium' model of anaphase chromosome movement. We conclude that, since single chromosomal fibre birefringence is not temperature-labile over most of the range of temperatures at which normal anaphase chromosome movement occurs, these data do not support the dynamic equilibrium model of anaphase chromosome movement.

Anaphase↗
PubMed2004

[Consolidation of cytoskeleton during plant spindle formation. II. "Fused spindles"].

Three mechanisms of fused spindle formation in meiosis of Solanacea have been described: 1) approach of daughter nuclei at prophase II; 2) fusion of perinuclear cytoskeleton systems at prophase II; 3) approach and fusion of prometaphase chaotic figures at prometaphase II. The process of fusion spindle formation appears to be complex and including several steps.

Cytoskeleton↗
PubMedDate not supplied

An automatic spindle analysis and detection system based on the evaluation of human ratings of the spindle quality.

An evaluation of the spindle quality ranked by experienced human raters is described. The pattern discriminating criteria obtained from this evaluation are used for an automatic system based on the complex demodulation method. The performance of this automatic system is compared with both the human visual scoring and with a second automatic system employing phaselocked loop techniques. The performance of the complex demodulation is satisfactory. It also detects spindles buried in slow waves. It is not sensitive to the age of the subject. This method opens possibilities for research of either temporal or chemical (drugs etc.) effects on the characteristics of rhythmic activity, without being hampered by the arbitrary EEG jargon "alpha" or "sigma".

Adult↗
PubMed2003

Centrosome and spindle pole body dynamics. Review and abstracts of the EMBO/EMBL Conference on Centrosomes and Spindle Pole Bodies, Heidelberg, September 13-17, 2002.

Five years after the first meeting held on Centrosomes and Spindle Pole Bodies, a second meeting was organized by Tano Gonzalez, Eric Karsenti, Kip Sluder, and Mark Winey in Heidelberg, Germany. Sponsored by the gracious European community (EMBO/EMBL), the meeting was both spectacular and exhausting. The wealth of information delivered, the plethora of model systems and unique approaches described, and the free exchange of information by a cooperative and excited community of scientists overwhelmed all participants. Even the best prepared scholars could not have anticipated the avalanche of data and insights that poured from the presentations from beginning to end. Daily posters by young and senior scientists added dimension to round out the well-planned series of presentations. The meeting began with opening remarks by Eric Karsenti and Michel Bornens who reminded participants of the historical questions of the field. Where does the centrosome come from? What are the mechanisms that control centrosome assembly and duplication? How is duplication coordinated with the cell cycle? Why do some cells have centrosomes, while others do not? What are the components of the centrosome? Does the centrosome play an important role in disease?

Animals↗
PubMed1994

Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body.

The Saccharomyces cerevisiae genes KAR1 and CDC31 are required for the initial stages of spindle pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calcium-binding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KAR1 (Vallen, E. A., W. Ho. M. Winey, and M. D. Rose. 1994. Genetics. In press), we wanted to determine whether Cdc31p and Kar1p physically interact. Cdc31p was expressed and purified from Escherichia coli and active for binding calcium. Using a protein blotting technique, Cdc31p bound to Kar1p in vitro via an essential domain in Kar1p required for SPB duplication (Vallen, E. A., M. A. Hiller, T. Y. Scherson, and M. D. Rose. 1992a. J. Cell Biol. 117:1277-1287). By immunofluorescence microscopy, we determined that the interaction also occurs in vivo. Cdc31p was localized to the SPB in wild-type cells but was mislocalized in a kar1 mutant strain. In a kar1 mutant containing a dominant CDC31 suppressor, Cdc31p was again localized to the SPB. Furthermore, the localization of Cdc31p to the SPB was affected by the overexpression of Kar1p-beta-galactosidase hybrids. Based on these data, we propose that the essential function of Kar1p is to localize Cdc31p to the SPB, and that this interaction is normally required for SPB duplication.

Calcium-Binding Proteins↗
PubMed1991

Genetic analysis of the relationships between the amoebal extranuclear spindle-organizing centre and the plasmodial intranuclear spindle-organizing centre of Physarum during conjugation.

Physarum amoebae possess an extranuclear spindle-organizing centre (abbreviated SPOC), located in a typical centrosome with a pair of associated centrioles while plasmodia possess an intranuclear SPOC without centrioles. In order to ascertain whether, during conjugation, the plasmodial SPOC is derived from the amoebal one or is not related to it, we have constructed amoebal strains possessing two and three SPOCs and we have used as a genetic marker the frequency of polycentric metaphases in order to evaluate the number of SPOCs in the plasmodia. The results of both symmetrical crosses, i.e. between amoebae possessing the same number of SPOCs, and asymmetrical crosses, i.e. between amoebae possessing a different number of SPOCs, show that: (1) the number of SPOCs in plasmodia is dependent upon the number of SPOCs in either one of the two parental amoeba; (2) in no cross does the number of plasmodial SPOCs equal the sum of the parental amoebal SPOCs, but it corresponds to that of only one parent without any polarity of transmission in asymmetrical crosses. These results are consistent with the following model: (1) plasmodial SPOCs are derived from the amoebal ones; and (2) one set of parental SPOCs is lost, destroyed or inactivated in the zygote.

Animals↗
PubMed1976

A spindle cell varient of thymic carcinoid tumor. A clinical, histologic, and fine structural study with emphasis on its distinction from spindle cell thymoma.

We describe the clinical, histologic, and fine structural features of two thymic carcinoid tumors that had a major spindle cell component, and present the reason for classifying our two cases as variants of thymic carcinoid tumors. These tumors pursued an aggressive clinical course, demonstrated histologic features such as vascular invasion and mitotic activity, ultrastructurally contained numerous dense-core granules, and lacked prominent tonofilaments and well-formed desmosomes. Radiotherapy, even for apparently encapsulated cases, may be indicated for this aggressive mediastinal neoplasm, which can be clearly separated from the thymoma by clinical, histologic, and fine structural criteria.

Adult↗