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Escape protein for beef cows: II. Source and level in ammoniated wheat straw-corn silage diets.

Two experiments, using Angus x Hereford spring-calving beef cows in mid- or late lactation nursing Simmental-sired calves, were conducted to evaluate the relative value of a corn gluten meal-blood meal mixture (CGM-BM; 50% of supplemental protein from each source). In Exp. 1 (78 d), cows in late lactation were assigned to one of three treatments: control at 8.2% CP (C), soybean meal at 10.4% CP (SBM), or CGM-BM at 10.3% CP. Diets were calculated to be isocaloric at 55% TDN. In Exp. 2 (65 d), cows in mid-lactation were assigned to four treatments: urea, SBM, low CGM-BM (LM), and high CGM-BM (HM). Diets in Exp. 2 were isonitrogenous (9.5% CP) and isocaloric (55% TDN). Diets in both experiments were based on ammoniated wheat straw and corn silage. Weight gains of cows and cow-calf pairs were greater (P less than .06) when protein was supplemented in Exp. 1. Gains were lower for cows fed urea (P less than .03) in Exp. 2 but were similar when cows were supplemented with SBM vs either the low or the high level of CGM-BM. Performance of calves did not differ among dietary treatments.

Animal Feed

Plant contents of magnesium, calcium and potassium in relation to ruminant nutrition.

Grass tetany and wheat pasture poisoning are metabolic diseases of mature, lactating beef cattle. In some other countries, similar problems occur with dairy cattle. In grass tetany, the animals generally are grazing cool-season forages in which Mg concentration or bioavailability of plant Mg is low. Levels of Mg in the blood serum also generally are low. Grass tetany can occur in beef cattle fed hay made from grass, small grains or alfalfa Medicago sativa L.). In wheat pasture poisoning where animals are grazing small grains forages, blood levels of Ca often are low, and blood Mg also may be low. Both grass tetany and wheat pasture poisoning occur when plants are growing rapidly in the spring, at the time of heavy lactation demand by ruminants for Mg and Ca. When the temperature increases and plants start to grow rapidly in the spring, concentrations of K, N, organic acids, and the ratio of K/(Ca + Mg) all increase, and the percent dry matter decreases. Much research remains to be done to understand all the soil, plant, and animal aspects of grass tetany and wheat pasture poisoning.

Animal Nutritional Physiological Phenomena

Genetic control of shikimate dehydrogenase in hexaploid wheat.

The genetics of shikimate dehydrogenase (SKDH; EC 1.1.1.25) was investigated in Triticum aestivum cv Chinese Spring (2n = 6x = 42; genomic formula ABD) using the zymogram technique. The enzyme occurs in two electrophoretically distinct forms on starch gels. The results of a study of aneuploid derivatives of Chinese Spring indicate that the SKDH isozyme of faster electrophoretic mobility is encoded by a gene, designated Skdh-A1, located in the p (= short) arm of chromosome 5A and that the products of two other genes, designated Skdh-B1 and Skdh-D1, located one each in the p arms of homoeologous chromosomes 5B and 5D, respectively, encode two isozymes of slower and coincident electrophoretic mobility. Additional evidence for this interpretation of the genetic basis of hexaploid wheat SKDH was obtained in studies of the SKDH zymogram phenotypes of various close relatives of hexaploid wheat, including T. monococcum, T. longissimum, T. tauschii, T. turgidum, and T. timopheevii.

Alcohol Oxidoreductases

Molecular analysis of an alcohol dehydrogenase (Adh) gene from chromosome 1 of wheat.

We have cloned and determined the nucleotide sequence of a gene encoding alcohol dehydrogenase (Adh) from Triticum aestivum cv. Millewa. Southern analysis using cv. Chinese Spring nullisomic-tetrasomic and ditelosomic lines established that the cloned gene mapped to the long arm of chromosome 1A and does not correspond to any previously identified wheat Adh locus. Southern analysis also provided evidence for triplicate copies of this Adh gene on the homoeologous group 1 chromosomes, while Northern blots indicated that the homoeologous group 1 Adh genes, like several other plant Adh genes, are transcribed under anaerobic conditions. Sequence analysis indicates that the cloned gene has a structure similar to both monocot and dicot Adh genes with an open reading frame encoding a polypeptide of 379 amino acids. Sequences important for eucaryotic gene expression such as the TATA box, polyadenylation signal, and intron splice sites were found in the expected positions. The open reading frame is interrupted by 8 introns which are in identical positions with 8 of the 9 introns in maize and pea Adh genes, suggesting that during evolution there are processes occurring that result in the loss of introns. Sequence analysis also revealed that the cloned wheat Adh gene shared extensive homology with the barley Adh3 gene not only in the coding region but also in the noncoding regions. However, this homology is discontinuous as a result of a 1.8-kbp insertion (TLM), which is present in the cloned wheat Adh gene and absent in the barley Adh3 gene. Sequence analysis of this insertion reveals features characteristic of the short terminal inverted repeat class of eucaryotic transposable elements. We have no evidence for the transposition of the TLM element. However, Southern blots reveal multiple copies of sequences related to TLM in the wheat genome and in other closely related species, suggesting that transposition may once have played an important role in the evolution of the Gramineae family.

Alcohol Dehydrogenase

Chromosome mapping of low-temperature induced Wcs120 family genes and regulation of cold-tolerance expression in wheat.

Low-temperature (LT) induced genes of the Wcs120 family in wheat (Triticum aestivum) were mapped to specific chromosome arms using Western and Southern blot analysis on the ditelocentric series in the cultivar Chinese Spring (CS). Identified genes were located on the long arms of the homoeologous group 6 chromosomes of all 3 genomes (A, B, and D) of hexaploid wheat. Related species carrying either the A, D, or AB genomes were also examined using Southern and Western analysis with the Wcs120 probe and the WCS120 antibody. All closely related species carrying one or more of the genomes of hexaploid wheat produced a 50 kDa protein that was identified by the antibody, and a Wcs120 homoeologue was detected by Southern analysis in all species. In the absence of chromosome arm 6DL in hexaploid CS wheat no 50 kDa protein was produced and the high-intensity Wcs120 band was missing, indicating 6DL as the location of Wcs120 but suggesting silencing of the Wcs120 homoeologue in the A genome. Levels of proteins that cross-reacted with the Wcs120 antibody and degrees of cold tolerance were also investigated in the Chinese Spring/Cheyenne (CS/CNN) chromosome substitution series. CNN chromosome 5A increased the cold tolerance of CS wheat. Densitometry scanning of Western blots to determine protein levels showed that the group 5 chromosome 5A had a regulatory effect on the expression of the Wcs120 gene family located on the group 6 chromosomes of all three hexaploid wheat genomes.

Adaptation, Physiological

A 'zebra' chromosome arising from multiple translocations involving non-homologous chromosomes.

An alloplasmic wheat line carrying a 'zebra' chromosome z5A was isolated from the derivatives of an Elymus trachycaulus x Triticum aestivum cv Chinese Spring hybrid. Chromosome z5A was named zebra because of its striped genomic in situ hybridization pattern. z5A consists of four chromosome segments derived from E. trachycaulus and four chromosome segments, including the centromere, from wheat. The short arm of z5A paired with the telocentric chromosome 1H(t)S of E. trachycaulus and the long arm with the long arm of normal 5A. z5A also carried several genetic markers derived from 1H(t)S. Chromosome 1H(t) was the only E. trachycaulus chromosome found in the sib plants of a previous generation from which z5A was derived. Monosomic 5A and telocentric chromosome 5AL were also found in most of the sib plants. The zebra chromosome most probably originated from spontaneous multiple translocations between chromosomes 5A and 1H(t)S or 5A and 1H(t).

Chromosome Banding

[RAPD markers for wheat chromosomes in Langdon disomic substitution lines].

Optimal conditions were developed for a random amplified polymorphio DNA (RAPD) assay of hexaploid bread wheat and tetraploid durum wheat. AmpliTaq Stoffcl fragment was found to be better than Taq DNA polymerase in generating RAPDs. Studies on chromosome specific RAPD markers of the A- and B- and D-genome were performed using the complete set of Langdon disomic substitution lines and the parental lines (Langdon and Chinese Spring) as templete. Seven out of twelve arbitrary primers (all Operon 10-mer sequences) yielded 13 products that could be assigned to 1.0 chromosomes of A- and B- and D-genome, five of 13 markers for A-genome (2A: J6a and J11b; 3A: D11b; 6A: J17; 7A: J15a), seven for B-genome (1B: J11c; 2B: D5, D11c and J18) and one for D-genome (1D: J11a). Using Chinese Spring ditelosomic lines, four RAPD markers were further mapped to a specific chromosome arm (i.e., J11b-2AL, J17-6AL, D11c-2BL, and J11a-1DL). This study demonstrates that reproduoible RAPID markers can be generated and assigned to wheat chromosomes except 4AL, using Langdon disomic substitution lines and Chinese Spring euploid and aneuploids as malerids.

Base Sequence

Effects of animal and supplement characteristics on average daily gain of grazing beef cattle.

Effects of animal gender and age, use of a growth stimulant, and supplementation with grain alone or grain plus other substances on ADG by growing beef cattle grazing bermudagrass paddocks with sod-seeded rye, wheat, and ryegrass were determined. Two grazing experiments (Exp. 1: late winter through mid-spring; Exp. 2: late spring through mid-summer) were conducted. Experiment 1 used 96, 12- to 13-mo-old Simmental-cross calves (heifers, 240 kg; steers, 272 kg), half of which were implanted with zeranol. Within each implant treatment, cattle received no supplement or .5% BW (DM) of ground corn alone or plus a mix of protein meals, zinc sulfate, thiamin-HCl, or salt. Daily gain was higher (P less than .05) with than without supplementation and was similar (P greater than .10) among supplement treatments. In Exp. 2, 96 crossbred beef steers, approximately 7 (230 kg) or 15 mo old (250 kg), were not supplemented (control) or received .5% BW (DM) of ground corn on d 1 to 84 (C-C), corn plus a protein meal mix on d 1 to 84 (CP-CP), corn on d 43 to 84 (O-C), corn plus the protein meal mix on d 43 to 84 (O-CP), or corn on d 1 to 42 and corn plus the protein meal mix on d 43 to 84 (C-CP). Daily gain on d 1 to 84 was affected (P less than .05) by supplement, age, implant, and the supplement x implant interaction (nonimplanted: .37, .56, .68, .40, .49, and .49; implanted: .37, .62, .54, .49, .70, and .71 kg for control, C-C, CP-CP, O-C, O-CP, and C-CP, respectively).

Adipose Tissue

Nitrate reductase activity (in vivo and in vitro) of ditelosomic stocks of wheat (Triticum aestivum L.).

The nitrate reductase activities (NRA) of 31 ditelosomic stocks were compared with that of the control plant [Chinese Spring (CS) euploid], using in vivo and in vitro assay procedures that had been optimized with respect to the euploid. Fourteen stock exhibited significant differences in in vivo NRA from that of the euploid; the effect of removal of a chromosome arm was always to increase NRA. Eight of these stocks showed similar effects in vitro, although in three, a casein-sensitive factor had to be eliminated before the difference was expressed. Homoeologous group effects were evident among ditelosomics of groups 2, 4, and 7, while for three chromosomes (2D, 7A, and 7B), removal of either are resulted in a similar increase in NRA in vivo and probable in vitro.

Genes

Genomic introgressions from wild relatives in the wheat genome alter meiotic dynamics in inter-varietal hybrids.

The use of wild relatives to introduce original diversity in the genome of bread wheat (Triticum aestivum L.) is an interesting approach to face the challenges of sustainable agriculture and the impact of climate change on wheat production. However, the influence of these wild-species introgressions on meiosis in inter-varietal wheat hybrids remains poorly understood. We analyzed the French wheat variety Renan (Re) carrying Aegilops ventricosa (Aev)-derived 2AS/2NS and 7DL/7DvL introgressions, the reference cultivar Chinese Spring (CS), which lacks these introgressions, and their inter-varietal hybrid Chinese Spring × Renan (CSRe). This analysis combined cytogenetic approaches with the assessment of reproductive performance. Furthermore, we generated a cytological atlas of meiosis in wild tetraploid Aev, quantifying bivalent configurations and chiasma frequency. We observed a reduced pollen viability and a slight decrease in floret fertility in the hybrid CSRe. Exploration of the meiotic behavior showed that CSRe exhibited increased numbers of rod bivalents and univalents, leading to a reduced average chiasma number and frequent chromosome bridges and fragmentations, whereas the parental lines maintained stable chromosome pairing. These rearrangements indicate that homologous chromosome pairing and recombination are affected in CSRe. We applied introgression-specific oligo-Fluorescent In Situ Hybridization to localize alien segments in CSRe, providing a novel strategy to investigate the meiotic behavior of introgressed regions. The 2AS/2NS introgressed segments in CSRe were frequently located on rod bivalents or univalents, while 7DL/7DvL segments consistently formed ring bivalents. Our results provide a foundation for guiding alien gene introgression and for understanding the behavior of chromosomes with introgressions in the wheat genome.

Meiosis

Influence of homoeologous chromosomes on gene-dosage effects in allohexaploid wheat (Triticum aestivum L.).

Gene-dosage responses for a group of six wheat endosperm proteins have been investigated by using compensated nulli-tetrasomic lines of cv. Chinese Spring. Practically linear dosage responses have been observed for all the proteins. However, for two of the proteins (and probably for a third one), the net output of protein, at each dosage of its structural gene, was 30-80% higher when the chromosome carrying an active homoeogene was absent. The possible significance of this effect in connection with the loss of gene redundancy undergone by polyploids is discussed.

Alleles

Dissecting the genetics of forage quality traits in soft red winter wheat in the U.S. southeast region.

Winter wheat plays a viable role in agriculture, not only as a primary grain crop but also as a valuable forage source that bridges fall-spring forage gaps in many regions, including the southeastern (SE) U.S. Despite its nutritive potential, genetic basis of forage-quality traits remains insufficiently understood, limiting breeding efforts for dual-purpose cultivars. This study aimed to dissect the genetic architecture of forage quality in 182 soft red winter wheat (SRWW) genotypes adapted to the SE U.S. using genome-wide association study (GWAS). Field experiments were carried out in randomized complete block design across three Georgia locations over two growing seasons (2023-2025), with forage sampled at the end of tillering and evaluated using near-infrared reflectance spectroscopy. Significant phenotypic variation was observed for dry matter (DM), crude protein (CP), acid and neutral detergent fiber (ADF, NDF), acid detergent lignin (ADL), total digestible nutrients (TDN), sugars (SUG), and relative forage quality (RFQ). Heritability estimates ranged from low-to-moderate in combined environments and from low-to-high within individual locations. Correlation analysis revealed strong positive associations among fiber-related traits and negative associations with TDN, RFQ, and SUG, while CP declined with increasing fiber. Genome-wide association analysis identified 282 significant marker-trait associations (P&#x2009;<&#x2009;1&#xd7;10-4) across 19 chromosomes, which were consolidated into 121 QTLs, including 27 major-effect QTLs. Three QTLs QRfq.uga-3B.1, QRfq.uga-3B.2 (RFQ) and QDm/Sug.uga-7A (DM, SUG) were stable across locations while QAdf/Adl.uga-2A (ADF, ADL) and QDm/Sug.uga-7A (DM, SUG) indicated multi-trait control. Notably, 25 of the 27 major QTLs were putatively novel, highlighting substantial untapped allelic diversity for forage-quality improvement in SE SRWW. Favorable allele accumulation resulted in an overall improvement in forage quality, increasing desirable nutritive traits (DM, RFQ, SUG, CP) while reducing undesirable traits (ADF, ADL). Candidate gene analysis linked six major QTLs with genes implicated in abiotic stress response, plant development, and metabolic regulation, supporting their functional relevance in forage-quality determination. Incorporating these loci into breeding programs provides a robust genetic framework for marker-assisted selection, enabling the development of dual-purpose wheat cultivars with enhanced forage quality, thereby strengthening wheat's utility as a reliable forage resource during periods of seasonal feed scarcity in SE production systems.

GWAS

Genetic control of NADH dehydrogenase-1 and aromatic alcohol dehydrogenase-2 in hexaploid wheat.

The genetic control of NADH dehydrogenase-1 (NDH-1) and aromatic alcohol dehydrogenase-2 (AADH-2) was investigated in Triticum aestivum cv. Chinese Spring. Evidence was obtained that NDH-1 is active as a monomer and is encoded by genes located in the p arms of the homoeologous group 4 chromosomes. The NDH-1 gene loci located in 4Ap, 4Bp, and 4Dp were designated Ndh-A1, Ndh-B1, and Ndh-D1, respectively. Aadh-A2 was previously reported to be located in 6Aq; in this study, Aadh-B2 and Aadh-D2 were localized in 6Bq and 6Dq, respectively. Alcohol dehydrogenase-1 is expressed on AADH-2 zymograms; the presence of a contaminating aliphatic alcohol in one or more reagents is suggested as the probable cause of this phenomenon.

Alcohol Oxidoreductases

Cow weights and reproduction on native rangeland and native rangeland-complementary forage systems.

Replicated cow herds of 15 Angus x Hereford (AH) and 15 Brahman x Hereford (BH) F1 cows each were placed on native rangeland (NR) or native rangeland-complementary forage (NRCF) systems. The NR was 8.1 ha of sagebrush-mixed prairie per cow-calf unit and the NRCF used the same mixed prairie complemented by .4 ha of double-cropped winter wheat and summer annual forage replacing about 30% of the mixed prairie for each cow unit. Calving seasons, spring for NR and fall for NRCF, were selected to complement the respective forage systems. Cow weights, reproductive performance, and population demographics were measured through seven calf crops to investigate their relationship to forage systems and cow breed types. Seasonal average cow weights increased from 484 +/- 7 (SE) kg during calf crop 1 to a peak weight of 568 +/- 8 kg at calf crop 5. Average weight of AH cows was 514 +/- 3 kg, compared with 539 +/- 3 kg for the BH cows (P < .01). Angus x Hereford and BH cows were 38 and 44 kg heavier, respectively, on the NRCF system than on the NR system. The average percentage of reproduction for AH cows was 90 +/- 2%, compared with 87 +/- 2% for the BH cows (P < .13). Both breeds had approximately 87 (AH) to 89% (BH) reproductive rate on the NR system. In contrast, AH cows had 94 +/- 2% reproduction on the NRCF system, compared with 84 +/- 2% for the BH cows.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed

Poisoning of Canada geese in Texas by parathion sprayed for control of Russian wheat aphid.

Approximately 200 Canada geese (Branta canadensis) died at a playa lake in the Texas Panhandle shortly after a winter wheat field in the basin adjacent to the lake was treated with parathion to control newly invading Russian wheat aphids (Diuraphis noxia). No evidence of infectious disease was diagnosed during necropsies of geese. Brain ChE activities were depressed up to 77% below normal. Parathion residues in GI tract contents of geese ranged from 4 to 34 ppm. Based on this evidence, parathion was responsible for the goose mortalities. Parathion applications to winter wheat will undoubtedly increase if parathion is applied for control of both Russian wheat aphids and greenbugs (Schizaphis graminum). Geese may potentially be exposed to widespread applications of parathion from fall to spring, essentially their entire wintering period.

Aerosols

Analyses of alpha/beta-type gliadin genes from diploid and hexaploid wheats.

The alpha/beta-gliadin genes isolated from both hexaploid wheat (cv. Yamhill) and the diploid A genome progenitor Triticum urartu had remarkably similar sequences and differ by only a few point mutations. Primer extension analysis indicated that the transcriptional start points for individual genes in the family cluster within a few nucleotides. Comparison of the promoter region of several alpha/beta-gliadin and B-hordein genes reveals two conserved regions at about -130 and -250 bp. DNA from the hexaploid cultivars, Cheyenne and Chinese Spring, and the diploid progenitors T. urartu and Aegilops squarrosa was analysed by Southern blotting. Restriction fragment lengths of the alpha/beta-gliadin genes varied only slightly between the various wheats, although the overall copy number varied significantly. A region between approx. -1700 and -700 bp upstream from the TATA box was highly repeated in all three wheat genomes. For the hexaploid-derived gene, over 1700 bp of sequence upstream from the TATA box was determined, revealing an additional open reading frame between approx. -1550 and -1250 bp relative to the gliadin TATA box. Northern blot analysis indicated that RNA homologous to this repeated sequence family was present only in developing seed and accumulated to a maximum at late stages of maturation.

Base Sequence

Overberg Research Projects: III. A preventive worm control programme for sheep in the Rûens, in the winter rainfall region of South Africa.

The mass gains and internal parasite burdens of a flock of untreated ewes and lambs were compared with those of a similar flock of treated ewes and lambs. These data, combined with climatic data, were used to compile a nematode parasite control programme for sheep in the Rûens, a sub-region of the winter rainfall area of South Africa. Teladorsagia were the most numerous parasites in the ewes and later in the season, also in the lambs. From July onwards, the development of Teladorsagia was inhibited regardless of previous exposure to worms, the number of worms present or the age of the host. Initially, Nematodirus dominated in the lambs and its development was not inhibited. Low numbers of Nematodirus, mainly inhibited, were found in the ewes. Both parasites were rife in winter but disappeared from the pasture during spring, while Trichostronglus rugatus, the dominant species of Trichostronglus, infested the lambs in spring and persisted in them throughout summer. Anthelmintic treatments had no significant effect on the live mass gains of ewes and suckling lambs while they were on lush, but heavily infested lucerne pastures for 5.5 months, but after a further 6 months on safe wheat stubble pastures, the overall mean live mass gain of the treated lambs was a significant 4.7 kg more than that of the untreated lambs. The integrated, preventive worm control programme recommended consists of pre-winter, pre-spring and pre-summer treatments with broad spectrum anthelmintics, but transfer to safe pastures after each treatment is essential.

Animals

Body and carcass composition of Angus and Charolais steers as affected by age and nutrition.

The objectives of this study were to examine the effects of a low vs moderate rate of gain during the growing phase on empty body and carcass composition during finishing of Angus and Charolais steers of two ages. Forty-eight Angus and 48 Charolais steers that were either spring-born (OLDER) or fall-born (YOUNGER) were fed two diets (alfalfa pellets [CON] or cubed grass-alfalfa hay, wheat straw, cottonseed hulls, and soybean meal [RES]) for a growing period followed by a conventional feedlot period. The feedlot period started when the YOUNGER-CON steers weighed the same as the OLDER-RES steers. At that time, an interaction of age x diet occurred in empty body fat content (P < .10), whereas breed and age x diet affected carcass fat content (P < .01). OLDER-CON steers were larger (average 378 kg empty BW) and fatter than the other, smaller groups (average 222 kg). Angus carcasses were fatter than Charolais carcasses (P < .01). At the end of the finishing phase, compensating steers (OLDER-RES) had fatter carcasses than OLDER-CON steers. Empty body fat content was affected by a breed x age x diet interaction (P < .10). Allometric regressions (Y = aXb) of fat on empty BW indicated that empty body fat accretion was greater in Angus than in Charolais and in YOUNGER than in OLDER steers. A breed x age x diet interaction (P < .10) indicated that OLDER-Angus had higher fat accretive rates than YOUNGER-Angus, whereas OLDER-CON-Charolais steers deposited fat more slowly than the remaining groups. These data suggest that steers receiving feedlot diets at light weights, whether young in age or previously restricted, accumulate fat more rapidly than do larger steers. This feeding strategy may be an advantage in late-maturing types, but moderate growth through approximately 75% of slaughter weight is recommended for early-maturing types.

Adipose Tissue