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At least 1,261 records · Page 70Linked to original sources

PubMed2000

Effects of skin cleaning modes on the condition of collagen and elastin after laser resurfacing.

BACKGROUND: Laser resurfacing is now widely used for the treatment of facial wrinkles, photoaging and acne scars and is usually combined with comparatively aggressive methods of cleaning detritus off the treated area after each laser pass. OBJECTIVE: This study aims to evaluate two different cleaning methods (damp gauze or damp large-tip cotton buds) in order to elucidate their influence on the healing process, if any. The left or right preauricular area, chosen at random on 10 patients undergoing periocular and perioral resurfacing with the same laser system, was cleaned after each laser pass with damp gauze (Group G) and the contralateral side with the large-tip cotton buds (Group C). Post-resurfacing oedema and erythema were observed in the preauricular areas and rated by the same experienced observer immediately after treatment and at 14, 30, 45, 60 and 90 days. RESULTS: Oedema was slightly more noticeable initially in Group G patients, but the erythema was more intense and lasted longer in the gauze-cleaned tissue. The sites were biopsied at 90 days and the samples sent for blinded histological evaluation to assess the degree of collagen and elastin fibre compaction. The histology demonstrated that the tissue from Group G achieved, at 90 days, better compaction of collagen and elastin fibres than that from Group C. CONCLUSIONS: It is suggested that the abrasive action caused by the gauze adds to the existing stimulative inflammatory reaction following CO2 laser resurfacing, which augments the collagen and fibre compaction response in the tissue. Comparatively aggressive cleaning with damp gauze may therefore be preferable to other methods when performing laser resurfacing.

Aged↗

PubMed1986

Conversion of proinsulin to insulin occurs coordinately with acidification of maturing secretory vesicles.

Proinsulin is a single polypeptide chain composed of the B and A subunits of insulin joined by the C-peptide region. Proinsulin is converted to insulin during the maturation of secretory vesicles by the action of two proteases and conversion is inhibited by ionophores that disrupted intracellular H+ gradients. To determine if conversion of prohormone to hormone actually occurs in an acidic secretory vesicle, cultured rat islet cells were incubated in the presence of 3-(2,4-dinitroanilino)-3' amino-N-methyldipropylamine (DAMP), a basic congener of dinitrophenol that concentrates in acidic compartments and is retained there after aldehyde fixation. The cells were processed for indirect protein A-gold colocalization of DAMP, using a monoclonal antibody to dinitrophenol, and proinsulin, using a monoclonal antibody that exclusively reacts with the prohormone. The average density of DAMP-specific gold particles in immature secretory vesicles that contained proinsulin was 71/micron 2 (18 times cytoplasmic background), which indicated that this compartment was acidic. However, the density of DAMP-specific gold particles in the insulin-rich mature secretory vesicle averaged 433/micron 2. This suggests that although proinsulin conversion occurs in an acidic compartment, the secretory vesicles become more acidic as they mature. Since the concentration of anti-proinsulin IgG binding in secretory vesicles is inversely proportional to the conversion of proinsulin to insulin, we were able to determine that maturing secretory vesicles had to reach a critical pH before proinsulin conversion occurred.

Animals↗

PubMed1992

A comparison of the fidelity of copying 5-methylcytosine and cytosine at a defined DNA template site.

5-Methylcytosine has been postulated to be an endogenous mutagen in procaryotes and eucaryotes leading to base substitution hot spots, C-->T transitions, resulting from spontaneous deamination of mC to T. The possibility remains, however, that a second mechanism involving mispairing of mC with A might also contribute to base substitution mutagenesis via G-->A transitions. Stimulation of the G-->A mutational pathway could involve preferential misincorporation of dAMP opposite template mC compared to C. To investigate this possibility, we synthesized a sequence containing mC at a defined template location. We compared the fidelity of copying mC versus C and the efficiency of extending mismatched base pairs at the mC position using three DNA polymerases, AMV reverse transcriptase, Drosophila DNA polymerase alpha, and mutant Escherichia coli Klenow fragment containing no proofreading exonuclease activity. Significant differences in misinsertion and mismatch extension efficiencies were observed only for the case of AMV reverse transcriptase. AMV reverse transcriptase was observed to incorporate dAMP 4 to 5-fold more efficiently opposite mC than C. Favored extension of a 5-MeC.A over C.A mispair was also observed with a difference of about 3-fold. In contrast to AMV reverse transcriptase, Klenow fragment showed no significant difference when copying either mC or C sites or when extending mispairs involving mC and C. Incorporation of dAMP opposite either C or mC was barely detectable using pol alpha, although pol alpha has been observed to form A.C mismatches in other sequences. While we cannot completely exclude the possibility that dAMP might be incorporated opposite mC in preference to C, our results suggest that contributions of the G-->A pathway to mC mutagenic hot spots are likely to be minor, lending additional support to the model invoking deamination of mC.

5-Methylcytosine↗

PubMed2005

Comparison of three lines of broiler breeders differing in ascites susceptibility or growth rate. 1. Relationship between acoustic resonance data and embryonic or hatching parameters.

Ascites is a prevalent cardiovascular disease among modern broilers with negative impacts on production and animal welfare. The peak of mortality due to ascites occurs at the end of the growing period, but the etiology of this problem may start during embryonic development. A few recent reports have demonstrated that the signs of ascites susceptibility are manifested during the late stages of incubation. In the current study, we used a nondestructive method based on egg acoustic resonance parameters [resonant frequency (RF) and damping] to establish a relationship between embryo physiological events during early development in broiler eggs and susceptibility to ascites. The hatching eggs of 3 broiler lines differing in ascites susceptibility were used for this study: ascites-resistant dam line (DAR), ascites-sensitive dam line (DAS), and ascites-sensitive sire line (SASL). These lines were selected on the basis of fast growth, high breast meat yield, and ascites induction at low temperatures such that the order of ascites susceptibility in terms of mortality was SASL >> DAS > DAR. Eggs were incubated under standard conditions in forced-draft incubators. We measured egg weights at setting, albumen pH, Haugh units (HU) at setting, and embryo weights at d 11 and 18, at internal pipping (IP), and at hatch. The durations of IP, external pipping (EP), and hatching were also determined. At 2 hourly periods during incubation, egg RF and damping were also measured. There were differences in egg weights between DAR and SASL vs. DAS, but albumen HU, albumen pH, and the ratio of yolk weight to egg weight were similar. There were differences in RF, damping, embryonic growth rates, and hatching events. Changes in resonant frequency and damping, which certainly suggest eggshell differences among lines, were not totally related to variations in physiological events during early and late embryonic development. A comparison between DAR and DAS, between DAS and SASL, or DAR and SASL indicates that sensitivity to ascites and selection for rapid growth rate in ascites-sensitive lines have different effects on embryonic parameters. We concluded that the sensitivity of broiler breeders to ascites does not influence egg internal quality, but the occurrence of ascites sensitivity in broilers could not be reliably predicted by early in ovo acoustic resonance parameters and hatching events.

Acoustics↗

PubMed1997

Characterization of muscarinic receptor subtypes that mediate antinociception in the rat spinal cord.

UNLABELLED: Spinal cholinergic receptors are involved in mediating antinociceptive effects. To characterize the receptor subtypes modulating nociceptive transmission, we first determined the antinociceptive effects of intrathecally administered muscarinic agonists (McN-A-343 and carbachol) and a cholinesterase inhibitor (neostigmine) in rats. The antagonist potencies of muscarinic antagonists with different preferences for muscarinic receptors [atropine, pirenzepine (M1), methoctramine (M2), and 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP; M3)] were then examined for McN-A-343, carbachol, and neostigmine. After determining the time of peak effect for each antagonist, the just maximally effective (JME) dose of each agonist was given in conjunction with one of the several doses of the antagonists. The three agents produced a dose-dependent increase in paw withdrawal latency, with the following 50% effective dose and the following JME doses: neostigmine 6 and 14 nmol, carbachol 29 and 110 nmol, and McN-A-343 354 and 630 nmol. The rank of order of potency (and median infective dose in nanomoles) for the antagonists was: neostigmine = (atropine 14 > 4-DAMP 44 > > methoctramine > 137, and pirenzepine > 236); carbachol = (pirenzepine 0.5 = atropine 0.6 > 4-DAMP 5 > > methoctramine > 137); McN-A-343 = (pirenzepine 0.5 > atropine 3 > 4-DAMP 6 > > methoctramine > 137). Our results suggest that the M1 and possibly the M3 muscarinic receptors mediate spinal antinociception in the rat. IMPLICATIONS: Spinal muscarinic agonists, such as carbachol and the cholinesterase inhibitor neostigmine, induce a potent analgesia in the rat. Using selective receptor antagonists, we have shown that these effects are likely mediated by spinal M1 and/or M3 receptor subtypes.

Analgesics↗

PubMed2005

Effect of mouthguards on the transmission of force across the human jaw.

OBJECTIVE: To determine if impact responses (stiffness and damping values) of the jaw and neck are significantly different between mouthguards of varying material and manufacturing properties, in controlled conditions involving specified upper limb orientation and applied loading. DESIGN: Quasi-experimental design. SETTING: Simon Fraser University biomechanical laboratory. PARTICIPANTS: Twelve male subjects between the ages of 19 to 28 participated in this study. All subjects were healthy, had no missing teeth, and had no mechanical (orthodontic) appliances. MAIN OUTCOME MEASUREMENTS: For each condition, the system stiffness and damping values were estimated using the free vibration technique. Stiffness and damping values are relevant because they are the major determinants of impact force and, potentially, injury risk. It is the first study to show experimentally the quantitative effects of mouthguard in situ. RESULTS: All the mouthguards lowered the system stiffness as compared with the no mouthguard condition (P < 0.001). There was no observed effect on stiffness between the 2 limb orientation positions. Excitation weight had an unexpected effect on system stiffness (P = 0.041), with increasing weight leading to increased stiffness. CONCLUSIONS: The findings suggest that wearing any mouthguard is better than wearing none at all due to their ability to reduce system stiffness and damping values after a blow to the chin.

Adult↗

PubMed2000

Evaluation of the smallest nonvanishing eigenvalue of the fokker-planck equation for the brownian motion in a potential. II. The matrix continued fraction approach

An equation for the smallest nonvanishing eigenvalue lambda(1) of the Fokker-Planck equation (FPE) for the Brownian motion of a particle in a potential is derived in terms of matrix-continued fractions. This equation is applicable to the calculation lambda(1) if the solution of the FPE can be reduced (by expanding the probability distribution function in terms of a complete set of appropriate functions) to the solution of a multiterm recurrence relation for the moments describing the dynamics of the Brownian particle. In contrast to the available continued fraction solution for lambda(1) [H. Risken, The Fokker-Planck Equation (Springer, Berlin, 1989)], this equation does not require one to solve numerically a high order polynomial equation. To test the theory, the smallest eigenvalue lambda(1) is evaluated for the FPE, which appears in the theory of magnetic relaxation of single domain (superparamagnetic) particles. Various regimes of relaxation of the magnetization in superparamagnetic particles are governed by a damping parameter alpha, the limiting values of which correspond to the high damping (alpha-->infinity) and the low damping (alpha<<1) limits in the theory of the escape rate over potential barriers. It is shown that for all ranges of the barrier height and damping parameters the smallest eigenvalue lambda(1) predicted by the continued fraction equation is in agreement with those gained by independent numerical methods and the asymptotic estimates for lambda(1) (in the high barrier limit) and, moreover, the matrix continued fraction approach may be successfully applied to the evaluation of lambda(1) in those ranges of parameters where traditional methods fail or are not applicable.

Journal Article↗

PubMed2000

Decay rates of internal waves in a fluid near the liquid-vapor critical point

We study the damping of internal waves in a viscous fluid near the liquid-vapor critical point. Such a fluid becomes strongly stratified by gravity due to its large compressibility. Using the variable-density incompressible Navier-Stokes equations, we model an infinite fluid layer with rigid horizontal boundaries and periodic side boundary conditions. We present operator-theoretic results that predict the existence of internal-wave modes with arbitrarily small damping rates. We also solve the eigenvalue problem numerically using a compound matrix shooting method and a second method based on a matched-asymptotic perturbation expansion for small viscosity. At temperatures far above the critical point, the damping of the internal waves is substantially influenced by both boundary layer and volumetric effects. The boundary layer effect is caused by horizontal shearing layers near the two fixed horizontal boundaries. As the temperature approaches the critical temperature, an additional internal shearing layer develops as the density stratification curve steepens on approach to the two-phase regime. Numerical calculations show that for some of the internal-wave modes this causes a dramatic increase in the damping rate that dominates the boundary layer effects.

Journal Article↗

PubMed2002

Role of long jumps in surface diffusion.

We analyze a probability of atomic jumps for more than one lattice spacing in activated surface diffusion. First, we studied a role of coupling between the x and y degrees of freedom for the diffusion in a two-dimensional substrate potential. Simulation results show that in the underdamped limit the average jump length scales with the damping coefficient eta as proportional, variant eta(-sigma(lambda)) with 1/2<or=sigma(lambda) less, similar 2/3, so that the diffusion coefficient behaves as D proportional, variant eta(-sigma) with 0<or=sigma less, similar 1/3. Second, we introduced a realistic friction coefficient for the phonon damping mechanism and developed the technique for Langevin equation with a velocity-dependent friction coefficient. The study of diffusion in this model shows that long jumps play an essential role for diffusing atoms of small masses, especially in two limiting cases, in the case of a large Debye frequency of the substrate, when the rate of phonon damping is low, and in the case of a small Debye frequency, when the one-phonon damping mechanism is ineffective.

Journal Article↗

PubMed2004

Multifrequency control of Faraday wave patterns.

We show how pattern formation in Faraday waves may be manipulated by varying the harmonic content of the periodic forcing function. Our approach relies on the crucial influence of resonant triad interactions coupling pairs of critical standing wave modes with damped, spatiotemporally resonant modes. Under the assumption of weak damping and forcing, we perform a symmetry-based analysis that reveals the damped modes most relevant for pattern selection, and how the strength of the corresponding triad interactions depends on the forcing frequencies, amplitudes, and phases. In many cases, the further assumption of Hamiltonian structure in the inviscid limit determines whether the given triad interaction has an enhancing or suppressing effect on related patterns. Surprisingly, even for forcing functions with arbitrarily many frequency components, there are at most five frequencies that affect each of the important triad interactions at leading order. The relative phases of those forcing components play a key role, sometimes making the difference between an enhancing and suppressing effect. In numerical examples, we examine the validity of our results for larger values of the damping and forcing. Finally, we apply our findings to one-dimensional periodic patterns obtained with impulsive forcing and to two-dimensional superlattice patterns and quasipatterns obtained with multifrequency forcing.

Journal Article↗

PubMed2006

Hydrodynamic Lyapunov modes in coupled map lattices.

In this paper, numerical and analytical results are presented which indicate that hydrodynamic Lyapunov modes (HLMs) also exist for coupled map lattices (CMLs). The dispersion relations for the HLMs of CMLs are found to fall into two different universality classes. It is characterized by lambda approximately k for coupled standard maps and lambda approximately k2 for coupled circle maps. The conditions under which HLMs can be observed are discussed. The role of the Hamiltonian structure, conservation laws, translational invariance, and damping is elaborated. Our results are as follows: (1) The Hamiltonian structure is not a necessary condition for the existence of HLMs. (2) Conservation laws or the translational invariance alone cannot guarantee the existence of HLMs. (3) Including a damping term in the system of coupled Hamiltonian maps does not destroy the HLMs. The lambda-k dispersion relation of HLMs, however, changes to the universality class with lambda-k2 under damping. In contrast, no HLMs survives in the system of coupled circle maps under damping. (4) An on-site potential destroys the HLMs. (5) The study of zero-value Lyapunov exponents (LEs) and associated Lyapunov vectors (LVs) shows that translational invariance and conservation laws play different roles in the tangent space dynamics. (6) The dynamics of the coordinate and momentum parts of LVs in Hamiltonian systems are related but different. Furthermore, numerical results for a two-dimensional system show that the appearance of HLMs in CMLs is not restricted to the one-dimensional case.

Journal Article↗

PubMed2006

Phonon dispersion of oriented DNA by inelastic x-ray scattering.

Films of oriented deoxyribonucleic acid (DNA), prepared by the wet spinning method, have been studied using inelastic x-ray scattering. Spectra were recorded within the range of energy transfers -30< homega <30 meV at momentum transfers homegaQ ranging from 2.5 to 30 nm(-1) whereby the direction of Q essentially coincided with the helical axis. Measurements at ambient temperature cover samples in the A, B, C, and D conformations of DNA. Within the limits of the instrumental resolution, the spectra were analyzed by the response of a damped harmonic oscillator delivering dispersion and damping of modes having displacements with nonzero projections onto Q, i.e., essentially the compression waves traveling along the helical axis. The longitudinal speed of sound resulting from the sinusoidal dispersion varies only weakly with conformation. Our sound speed values are compared to results from Brillouin spectroscopy. The dispersion curves exhibit a minimum at about the inverse rise per residue, which -- together with strong elastic scattering -- reflect the large degree of disorder. Overdamping of the modes is observed for Q>5 nm(-1). The possibility that the observed large damping parameters are due to several contributing modes is discussed in terms of a simple model calculation for an idealized double helix. Whereas the quasicrystalline approximation for an effective disordered chain could well describe the sinusoidal dispersion, it fails to reproduce the observed damping by one order of magnitude. Our results indicate that the high-frequency dynamics of DNA is liquidlike and is most appropriately described by instantaneous normal modes of short correlation length.

DNA↗

PubMed2006

Experimental investigation of the dynamics of a vibrating grid in superfluid 4He over a range of temperatures and pressures.

In an earlier paper [Nichol, Phys. Rev. E, 70, 056307 (2004)] some of the present authors presented the results of an experimental study of the dynamics of a stretched grid driven into vibration at or near its resonant frequency in isotopically pure superfluid 4He over a range of pressures at a very low temperature, where the density of normal fluid is negligible. In this paper we present the results of a similar study, based on a different grid, but now including the temperature range where the normal fluid density is no longer insignificant. The new grid is very similar to the old one except for a small difference in the character of its surface roughness. In many respects the results at low temperature are similar to those for the old grid. At low amplitudes the results are somewhat history dependent, but in essence there is no damping greater than that in vacuo. At a critical amplitude corresponding to a velocity of about 50 mms(-1) there is a sudden and large increase in damping, which can be attributed to the generation of new vortex lines. Strange shifts in the resonant frequency at intermediate amplitudes observed with the old grid are no longer seen, however they must therefore have been associated with the different surface roughness, or perhaps were due simply to some artifact of the old grid, the details of which we are currently unable to determine. With the new grid we have studied both the damping at low amplitudes due to excitations of the normal fluid, and the dependence of the supercritical damping on temperature. We present evidence that in helium at low amplitudes there may be some enhancement in the effective mass of the grid in addition to that associated with potential flow of the helium. In some circumstances small satellite resonances are seen near the main fundamental grid resonance, which are attributed to coupling to some other oscillatory system within the experimental cell.

Journal Article↗

PubMed2000

Large nonlinear dynamical response of superparamagnets: interplay between precession and thermoactivation in the stochastic landau-lifshitz equation

The nonlinear dynamical response of classical spins governed by the stochastic Landau-Lifshitz equation is found to be large and very sensitive to the damping in the medium-to-weak damping regime. This result is interpreted in terms of a cooperation, induced by the driving field, between the precession of the spin and its thermoactivation over the potential barrier. The large damping dependence (absent in the linear response) can be used to determine the evasive damping coefficient in superparamagnets, so clarifying the nature of the spin-environment interaction in these systems.

Journal Article↗

PubMed2001

Evidence for a quasi-two-dimensional proton glass state in Cs5H3(SO4)(4); xH(2)O Crystals.

We describe damping of hypersonic and ultrasonic longitudinal acoustic (LA) phonons in crystals of Cs 5H (3)(SO (4))(4);xH 2O (PCHS) between 100 and 360 K. The damping of LA phonons exhibits strong dispersion caused by relaxation processes in the region of transformation into the glasslike phase (T(g) approximately 260 K). Near T(g) the damping of ultrasonic phonons propagating in the basal plane reflects the cooperative freezing of acid protons. The damping of LA phonons propagating perpendicular to the basal plane can be fit by the Debye model and is due to the interaction between protons and LA phonons. This suggests that the proton glass state that is realized at T<T(g) has a quasi-two-dimensional nature.

Journal Article↗

PubMed2005

Strongly inhibited transport of a degenerate 1D Bose gas in a lattice.

We report the observation of strongly damped dipole oscillations of a quantum degenerate 1D atomic Bose gas in a combined harmonic and optical lattice potential. Damping is significant for very shallow axial lattices (0.25 photon recoil energies), and increases dramatically with increasing lattice depth, such that the gas becomes nearly immobile for times an order of magnitude longer than the single-particle tunneling time. Surprisingly, we see no broadening of the atomic quasimomentum distribution after damped motion. Recent theoretical work suggests that quantum fluctuations can strongly damp dipole oscillations of a 1D atomic Bose gas, providing a possible explanation for our observations.

Journal Article↗

PubMed2005

Time resolved magnetization dynamics of ultrathin Fe(001) films: spin-pumping and two-magnon scattering.

The time-resolved magnetic response of ultrathin epitaxial Fe(001) films grown on GaAs(001) and covered by Au, Pd, and Cr capping layers was investigated by time and spatially resolved Kerr effect measurements. The magnetization was excited by an in-plane magnetic field pulse using the transient internal field generated at a Schottky barrier while the wavelength of the excitation (resonant mode) was roughly 4 microm. Each of the three cap layers affected the spin relaxation in a unique way. Au cap layers resulted in the bulk Gilbert damping of the Fe film. Pd cap layers caused an additional Gilbert damping due to spin-pump or spin-sink effects. Cr cap layers lead to a strong extrinsic damping which can be described by two-magnon scattering. In this case the strength of the extrinsic damping can be controlled by a field induced shift of the spin wave manifold with respect to the excited k vector.

Journal Article↗

PubMed2005

Aberration correction by nonlinear beam mixing: generation of a pseudo point sound source.

Nonlinear beam mixing with microbubbles was explored to create a pseudo point source for aberration correction of therapeutic ultrasound. A damping coefficient for a bubble driven by a dual frequency sound field was derived by revisiting Prosperetti's linearized damping model. As a result, the overall damping term for dual frequency was obtained by linear summation of two damping terms for each frequency. The numerical simulation based on the bubble model suggests that the most efficient size range to generate a 1 MHz frequency from 4 MHz and 5 MHz sound sources is 2.6 to 3.0 microm. Furthermore, this size range constitutes the primary distribution of a specific ultrasound contrast agent. When a chamber of 0.1% of the diluted agent is sonified by 4 MHz and 5 MHz sound beams with 80 degrees incident angle between them, an approximately 100 Pa, 1 MHz difference frequency signal can be measured approximately 10 cm away. In addition, the received 1 MHz difference frequency signal shows omni-directional characteristics, even though the overlap zone of the two sound beams is on the order of the difference frequency wavelength. Therefore, the induced sound source can be considered as a pseudo point source and is expected to be useful for aberration correction for therapeutic ultrasound.

Animals↗