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Comparison of mechanical performance among different frame configurations of the Ilizarov external fixator: experimental study.

The circular frame of the Ilizarov fixator can be modified to reflect anatomic variations and treatment aims. However, these modifications in the frame system cannot always achieve the mechanical performance of the standard frame system. A standard system has two rings in each bone fragment connected by four longitudinal bars on each side of the ring. In this study, the mechanical performances of one standard and eight modified frame systems were compared. Each system was loaded on a material testing machine, with calculation of axial compression, four-point bending, and torsion. As a result, systems that were modified with drop wires and Schanz screws 45 degrees oblique to the wires on the proximal ring provided a mechanical performance closer to the standard system than systems with other modifications.

Biomechanical Phenomena↗

Study of Staphylococcus aureus adhesion on a novel nanostructured surface by chemiluminometry.

In recent years the progress in the field of nanotechnologies has offered new possibilities to control the superficial features of implant materials down to a nanoscale level. Several studies have therefore tried to explore the effects of nanostructured biomaterial surfaces on the behavior of eukaryotic cells. However, nanotopography could exert an influence also on the behavior of prokaryotic cells, with relevant implications concerning the susceptibility of implant surfaces to infection. Aim of this study was to examine the behavior of Staphylococcus aureus on polyethylene terephthalate (PET) surfaces either cylindrically nanostructured (PET-N) or flat ion-etched (PET-F), and on tissue culture-grade polystyrene (PS). Microbial adherence was assessed by chemiluminometry under 4 different conditions: (a) bacteria suspended in MEM medium, (b) bacteria in MEM supplemented with 10% fetal bovine serum (FBS), (c) test surfaces preconditioned in FBS, and (d) post-exposure of colonised surfaces to serum-supplemented MEM. Under all circumstances, PET-F and PET-N specimens showed identical bacterial adhesion properties. In the absence of serum, all 3 test materials showed a very high adhesivity to microbial cells and both PET surfaces exhibited greater adhesion than PS. On the contrary, the presence of 10% serum in solution significantly affected cell behavior: the number of microbial cells on all surfaces was drastically reduced, and the adhesion properties of PET surfaces with respect to PS were reversed, with PET being less adhesive. Overall, the specific cylindrical nanostructures created on PET did not significantly influence microbial behavior. Ongoing studies are verifying whether other nanotopographies with different geometry could have more substantial effects.

Bacterial Adhesion↗

Mechanism of disc rupture. A preliminary report.

Lumbar intervertebral disc herniation is thought to be related to senescent changes in the nucleus pulposus except in rare instances of trauma. This investigation provides the first in vitro model of disc prolapse that reliably ruptures discs under physiologically reasonable stress. Fourteen vertebral motion segments with intact posterior elements were loaded repetitively at 1.5 Hz in a combination of flexion (7 degrees), rotation (less than 3 degrees), and compression (1,334 N) for an average of 6.9 hours (range, 3.0-13.0 hours) in a materials testing machine. Loading was terminated when reaction force leveled off for more than 1 hour. Ten discs failed through annular protrusions, and four failed by nuclear extrusion through annular tears, supporting the hypothesis that intervertebral disc prolapse is peripheral in origin. The annulus fibrosus is the site of primary pathologic change.

Humans↗

Short fiber reinforced composite: the effect of fiber length and volume fraction.

AIM: The aim of this study was to determine the effect of short fiber volume fraction and fiber length on some mechanical properties of short fiber-reinforced composite (FRC). METHODS AND MATERIALS: Test specimens (2 x 2 x 25 mm3) and (9.5 x 5.5 x 3 mm3) were made from short random FRC and prepared with different fiber volumes (0%-22%) and fiber lengths (1-6 mm). Control specimens did not contain fiber reinforcement. The test specimens (n=6) were either dry stored or thermocycled in water (x10.000, 5-55 degrees C) before loading (three-point bending test) according to ISO 10477 or statically loaded with a steel ball (Ø 3.0 mm) with a speed of 1.0 mm/min until fracture. A universal testing machine was used to determine the flexural properties and the load-bearing capacity. Data were analyzed using analysis of variance (ANOVA) (p=0.05) and a linear regression model. RESULTS: The highest flexural strength and fracture load values were registered for specimens with 22 vol% of fibers (330 MPa and 2308 N) and with 5 mm fiber length (281 MPa and 2222 N) in dry conditions. Mechanical properties of all test specimens decreased after thermocycling. ANOVA analysis revealed all factors were affected significantly on the mechanical properties (p<0.001). CONCLUSIONS: By increasing the volume fraction and length of short fibers up to 5 mm, which was the optimum length, the mechanical properties of short FRC were improved.

Analysis of Variance↗

Effect of metal surface topography on mechanical bonding at simulated total hip stem-cement interfaces.

Bonding and loosening mechanisms between bone cement and joint prostheses have not been well identified. In this study, the effects of simulated hip stem surface topography on the interfacial shear strength were examined. Six different surface topographies were used. They were described by several surface characterization parameters that may directly relate to the interfacial bonding strength: average surface roughness R(a), root mean square slope R(Deltaq), correlation length beta, and fluid retention index R(ri). The shear strengths between Palacos E bone cement and stainless steel rods were measured using an Instron materials testing machine. We found that cement can "flow" into the surface microtopography and establish good contact with the metal surface. The results show that the interfacial strength increases monotonically with the increase of R(Deltaq) instead of with R(a). The relationship between interfacial strength and surface parameters shows that a metal stem with an isotropic surface texture, higher R(Deltaq), and greater R(ri) gives a higher interfacial strength.

Biocompatible Materials↗

The degree of conversion of fiber-reinforced composites polymerized using different light-curing sources.

OBJECTIVES: Fiber-reinforced composites (FRC) with different polymer matrix compositions are available on the market. The aim of this study was to compare the degree of monomer conversion of FRC that contained either dimethacrylate resin matrix or semi-IPN resin matrix. In addition, the effect of different types of light-curing units and durations of light irradiation on the degree of conversion was evaluated. MATERIALS AND METHODS: Continuous unidirectional fibers impregnated with dimethacrylate or semi-IPN resin were compared. As control materials, a particulate filler composite resin and an unfilled resin were used. The materials were photopolymerized using various irradiation times and different types of light-curing units (quartz-tungsten-halogen with standard or turbo tip, light-emitting diode and plasma-arc). Degree of monomer conversion (DC%) was determined by FT-IR spectrometry. The infrared spectra were recorded at seven different time points after polymerization. RESULTS: ANOVA showed significant differences between the materials tested, and the curing units (P<0.05). The DC% of semi-IPN and dimethacrylate matrix FRC was not statistically different (P>0.05) compared to that of unfilled resin. Conventional quartz-tungsten halogen with turbo tip showed the highest DC% (69-72.3%), whereas the plasma-arc curing unit showed the lowest degree of conversion (47-62%). SIGNIFICANCE: Within the limitations of this study, it can be concluded that semi-IPN matrix of FRC did not show a difference in the degree of conversion compared to dimethacrylate resin FRC when quartz-tungsten or a light-emitting diode polymerization unit was used. The plasma-arc light-curing unit showed a lower degree of conversion with all materials compared to the light-emitting diode and quartz-tungsten-halogen lamps.

Analysis of Variance↗

New method for the in vitro evaluation of dental alloy bonding systems.

STATEMENT OF PROBLEM: Bonding systems are used in some fixed prosthetic devices with base alloys. However, different studies of the same dental alloy bonding agents, under similar circumstances, have yielded disparate results in bond strength testing. PURPOSE: This study compared directly 2 dental alloy bonding systems through a "duel" type of confrontation, which basically is a 2-way tensile force test. MATERIAL AND METHODS: Ninety Wiron 88 base alloy cylinders (diameter of 8 mm length 15 mm) were sandblasted on both sides with Al(2)0(3) powder (particle size 50 microm) during 10 seconds at an approximate distance of 5 mm, at an air pressure of 60 psi determined before sandblasting procedures. The surface of each cylinder was cleaned from Al(2)0(3) powder with a strong burst of oil-free air from a chairside air syringe. Thirty cylinders were randomly assigned to 1 of 3 groups for direct bond strength comparison: (1) Panavia 21 to Panavia EX, (2) Panavia 21 to Metabond, or (3) Panavia 21 to a combination of a resin bonding agent plus Panavia 21. Each group was composed of 10 specimens that used 3 cylinders for each specimen. Each side of the sample cylinder received the same quantity of cement and 1 cylinder at a time was bonded to it. Cylinder alignment was verified with a Boley gauge during luting procedures. The bonded 3-piece block was held together for 24 hours under a compressive force of 2 kg/cm(2) using a hydraulic press. Excess cement was removed with a brush, and the pertinent air sealant was applied to allow for autocuring of the cement. Specimens were later stored in water at room temperature for 48 hours before thermocycling procedures. Each specimen was thermocycled for 100 cycles with a 5-minute dwelling time in water at 4 degrees C and 60 degrees C. Specimens were subject to tensile force testing until debonding in 1 of the cylinders. RESULTS: The opposing pull duel test (OPDT) showed that the Panavia EX failed (40. 3 MPa) 10 of 10 duels against Panavia 21, whereas Panavia 21 failed (49.7 MPa) 9 of 10 duels against Metabond, and Panavia 1 failed (50. 1 MPa) 10 of 10 duels against Photobond+Panavia 21. ANOVA revealed significant differences (P <.05) between PAN-EX group and MET and PHB+P21 groups. However, no significant differences were found between MET and PHB+P21 groups. CONCLUSION: The opposing pull duel test was a valid method to directly compare bond strengths of 2 bonding systems to dental base alloys. There was a small dispersion of the values even though cement mixing and thickness variables were difficult to control. Duel tensile testing provides meaningful information on the superiority of one bonding system over another in this controlled environment.

Analysis of Variance↗

A study on the radiopacity of different shades of resin-modified glass-ionomer restorative materials.

There are several resin-modified glass-ionomer restorative materials available to the dental profession today. The commercially available brands are presented in a range of shades. There is little information on their radiopacity and whether this varies with differences in shade. While the general radiopacity of various products may have been studied, only assumptions are available regarding their consistency between shades. The purpose of this study was to investigate if there were any significant differences in the radiopacity of the shades available within each commercial product. The products evaluated were Fuji II LC, Vitremer, and Photac-Fil. The optical densities of standardized radiographs of samples of these materials were determined and radiopacity values of materials expressed in millimeter equivalents of aluminum. Of the three resin-modified glass-ionomer restorative materials tested, Fuji II LC was the most radiopaque and Photac-Fil the least. Fuji II LC and Vitremer showed radiopacity values equivalent to > 2.5 mm and > 1.5 mm aluminum respectively; Photac-Fil demonstrated very low radiopacity values (equivalent to < 0.6 mm aluminum). Statistical analysis revealed that there was no significant difference in radiopacity among the shades within each of these brands.

Aluminum↗

Arthroscopic knots: determining the optimal balance of loop security and knot security.

PURPOSE: The purpose of this study was to determine the optimal knot configuration that maximized both knot and loop security when tied with 2 different types of nonabsorbable, braided suture. TYPE OF STUDY: In vitro biomechanical study. METHODS: Six commonly used arthroscopic sliding knots (Duncan loop, Nicky's knot, Tennessee slider, Roeder knot, SMC knot, Weston knot) with and without a series of 3 reversing half-hitches on alternating posts (RHAPs) as well as a static surgeon's knot were tied. Two different nonabsorbable, braided sutures were used, and a total of 7 knots were tied for each possible combination of knots and sutures, for a total of 182 knots. Each knot was tied around a 30-mm circumference post to assure a consistent loop circumference of 30 mm before "locking" the complex sliding knots by tensioning the wrapping limb of the suture. Each loop was mounted on a Material Testing System machine, and its circumference was measured at a 5-N preload to assess each knot's ability to maintain a tight suture loop without slippage (loop security). Knot security was measured as the maximum force to failure at 3 mm of crosshead displacement or suture breakage during single-pull load testing. RESULTS: The surgeon's knot provided the highest force to failure and the tightest loop circumference whether tied with No. 2 Ethibond (Ethicon, Somerville, NJ) or No. 2 Fiberwire (Arthrex, Naples, FL) suture. Among the sliding knots, the Roeder knot with 3 RHAPs showed the best balance of loop security and knot security when tied with No. 2 Ethibond or No. 2 Fiberwire. Sliding knots tied without RHAPs showed low force to failure and loose suture loops whether tied with Ethibond or Fiberwire. The addition of 3 RHAPs improved knot security and, in most cases, loop security of all the sliding knots. When tying a static surgeon's knot or a sliding knot with RHAPs, using No. 2 Fiberwire increased the force to failure over comparable knots tied with No. 2 Ethibond. All knots failed by a combination of knot slippage and suture stretch. When using No. 2 Ethibond, securing most sliding knots with 3 RHAPs or tying a surgeon's knot changed the failure mechanism from knot slippage to suture stretch, suggesting that the maximum knot holding capacity of No. 2 Ethibond had been achieved when tying these knot configurations. However, even at failure forces twice that achieved with No. 2 Ethibond, suture slippage continued to occur with sliding knots with 3 RHAPs using No. 2 Fiberwire. This indicates that the maximum knot-holding capacity of No. 2 Fiberwire had not been achieved, and that further knot configurations should be tested. CONCLUSIONS: (1) A static surgeon's knot provides the best balance of loop security and knot security within the knot configurations tested in this study. (2) A sliding knot without RHAPs has both poor loop security and knot security and should not be tied. (3) The addition of 3 RHAPs improves knot security of all sliding knots tested and improves loop security of most of the sliding knots tested. (4) The addition of 3 RHAPs improves the knot security of all sliding knots to adequately resist predicted in vivo loads. (5) The Roeder knot with 3 RHAPs provides the best balance of loop security and knot security within the sliding knot configurations tested in this study regardless of suture type. (6) Tying a surgeon's knot or a sliding knot with 3 RHAPS using No. 2 Fiberwire increases knot security over the same knot tied with No. 2 Ethibond. CLINICAL RELEVANCE: This study identifies the static and sliding configurations of commonly used arthroscopic knots in order to aid the surgeon in choosing the most biomechanically effective knot for use in arthroscopic surgery.

Arthroscopy↗

Comparison of two fixation methods of oblique lesser metatarsal osteotomies: a biomechanical study.

Two methods of internal fixation of oblique lesser metatarsal osteotomies were compared biomechanically using fresh-frozen human cadaver bones. Osteotomies were made obliquely through the metatarsal shafts and fixed with either crossed Kirschner wires or a single AO screw using the lag technique. The specimens were then fixed at their proximal end and loaded to failure using an axial torsion material testing system (MTS, Minneapolis, MN). Load displacement curves were obtained and the stiffness of the constructs were determined. Single-screw fixation was found to be significantly stiffer than the crossed wire configuration (P < .01). Single-screw fixation resulted in a stiffness of 211.2 +/- 111.7 N/cm (mean +/- SD), while stiffness of the crossed wire configuration averaged 56.9 +/- 25.1 N/cm.

Biomechanical Phenomena↗

[Effect of saliva contamination on microleakage around class-5 cavities restored with three different types of adhesive materials].

The recent improvement of adhesive materials should decrease the risk related to saliva contamination. The aim of this study was to evaluate the effect of saliva contamination on the microleakage within class V cavities restored with three different types of materials: conventional glass ionomer cement, composite resin and compomer. 30 human extracted teeth were randomly divided in 3 equal groups. In each group, class V cavities were prepared on both facial and lingual surfaces (but joint for glass ionomer cement and bevelled incisal margin for composite resin and compomer). The lingual cavities were contaminated with saliva prior to restoration, while the facial cavities were not contaminated, serving as control. After water storage for 24 hours, teeth were immersed in 1% methylene blue solution for 24 hours. The axial sections were viewed under an optical microscope and the extent of dye penetration along cervical, axial and incisal margins was measured in millimetres. Statistic analysis showed that under salivary contamination, microleakage increased along the cervical margin of restoration for all three tested materials. Saliva contamination resulted in microleakage within the axial wall of the cavity only for the conventional glass ionomer cement. These data indicate that composite resin and compomer used together with new adhesives seem to be less sensitive to saliva contamination compared to conventional materials. However, under saliva contamination, cervical microleakage cannot be completely prevented and proper isolation should still be mandatory.

Adhesives↗

Immediate performance of self-etching versus system adhesives with multiple light-activated restoratives.

OBJECTIVES: The purpose of this study was to evaluate the performance of both single and double applications of (Adper Prompt L-Pop) self-etching dental adhesive, when used with three classes of light-activated restorative materials, in comparison to the performance of each restorative system adhesive. Evaluation parameters to be considered for the adhesive systems were (a) immediate marginal adaptation (or gap formation) in tooth cavities, (b) free setting shrinkage-strain determined by the immediate marginal gap-width in a non-bonding Teflon cavity, and (c) their immediate shear bond-strengths to enamel and to dentin. METHODS: The maximum marginal gap-width and the opposing-width (if any) in the tooth cavities and in the Teflon cavities were measured immediately (3 min) after light-activation. The shear bond-strengths to enamel and to dentin were also measured at 3 min. RESULTS: For light-activated restorative materials during early setting (<3 min), application of Adper Prompt L-Pop exhibited generally superior marginal adaptation to most system adhesives. But there was no additional benefit from double application. The marginal-gaps in tooth cavities and the marginal-gaps in Teflon cavities were highly correlated (r = 0.86-0.89, p < 0.02-0.01). For enamel and dentin shear bond-strengths, there were no significant differences between single and double applications, for all materials tested except Toughwell and Z 250 with enamel. SIGNIFICANCE: Single application of a self-etch adhesive was a feasible and beneficial alternative to system adhesives for several classes of restorative. Marginal gap-widths in tooth cavities correlated more strongly with free shrinkage-strain magnitudes than with bond-strengths to tooth structure.

Acid Etching, Dental↗

Effect of seating force on film thickness of new adhesive luting agents.

This study examined the effect of seating force on the film thickness of new adhesive luting agents. The method was in compliance with American National Standards/American Dental Association Specification No. 8 for zinc phosphate cement. The materials tested were zinc phosphate cement, glass ionomer cement, polycarboxylate cement, and a resinous cement with a dentinal bonding agent. All materials were manipulated exactly as described in the manufacturer's instructions, and an electronic gauge with an accuracy of 0.5 micron was used. Each class of material was measured 10 times at six different seating forces. Analysis of variance and multiple comparisons testing disclosed that the seating force and class of material strongly influenced the film thicknesses of luting agents. A mathematical model describing the response of zinc phosphate cement within the range tested was reported.

Analysis of Variance↗

A biomechanical comparison between the central one-third patellar tendon and the residual tendon.

The purpose of this study was to compare the tensile strength of the central one third patellar tendon--as used for reconstruction of the anterior cruciate ligament--to that of the residual patellar tendon. Ten matched pairs of human cadaveric knees were used for this study, each specimen consisting of an intact patella-patellar tendon-proximal tibial unit. One knee from each pair was randomly selected for removal of both the medial and lateral one third of the patellar tendon, leaving the central one third intact. The contralateral knee of each pair underwent removal of the central one third of the patellar tendon, leaving the residual two thirds intact. Each specimen was then mounted in a materials testing machine and tensile tested to failure at a strain rate of 100%.s-1. The most important result to emerge from these experiments was that there was no significant difference in maximum force to failure for the residual patellar tendon compared to the central one third. Thus any thought that removal of the central one third as a graft still leaves a tendon twice as wide and therefore twice as strong as a graft is dispelled by these experiments.

Adult↗

A study of the bioactive bone cement--bone interface: quantitative and histological evaluation.

The interface between bone and a bioactive glass cement--a mixture of bioactive glass powder and ammonium phosphate solution, previously reported on by the authors--was evaluated quantitatively and histologically. The materials tested were (1) the original bioactive glass cement (BCI cement); (2) an improved type of bioactive glass cement (BCII cement); (3) polymethylmethacrylate (PMMA) bone cement; and (4) a bioactive, apatite-wollastonite-containing, glass ceramic (A-WGC). Hardened cylindrical specimens of each cement were inserted loosely into canine femora and the interfacial shear strengths were measured using a push-out test. The interfacial strength values of the bioactive glass cements increased with prolonged implantation time. At each postimplantation time studied (8, 12, and 24 weeks), the interfacial strength value of BCI cement did not differ significantly from that of A-WGC. BCII cement interfacial strength was greater than that of BCI cement, whereas the interfacial strength of PMMA bone cement remained at a very low level throughout the study. Histological examinations revealed that direct bonding of both bioactive glass cements to bone had occurred without pathologic degradation. After 24 weeks, the defects between the bone and the bioactive glass cements had been filled with mature lamellar bone. Because the bioactive glass cement system developed by the authors, especially BCII cement, shows excellent osteoconductivity and bonds to bone tightly, we consider it to be a promising material for fixing prostheses into bone.

Animals↗

Tensile strength of the medial patellofemoral ligament before and after repair or reconstruction.

The tensile strength of the medial patellofemoral ligament (MPFL), and of surgical procedures which reconstitute it, are unknown. Ten fresh cadaver knees were prepared by isolating the patella, leaving only the MPFL as its attachment to the medial femoral condyle. The MPFL was either repaired by using a Kessler suture or reconstructed using either bone anchors or one of two tendon grafting techniques. The tensile strength and the displacement to peak force of the MPFL were then measured using an Instron materials-testing machine. The MPFL was found to have a mean tensile strength of 208 N (SD 90) at 26 mm (SD 7) of displacement. The strengths of the other techniques were: sutures alone, 37 N (SD 27); bone anchors plus sutures, 142 N (SD 39); blind-tunnel tendon graft, 126 N (SD 21); and through-tunnel tendon graft, 195 N (SD 66). The last was not significantly weaker than the MPFL itself.

Aged↗

Compressive and diametral tensile strengths of current adhesive luting agents.

Strength parameters greatly influence the selection of luting agents. This study compared the compressive and diametral tensile strengths of six classes of new adhesive luting agents (ALAs) with zinc phosphate as the controls. The 11 materials tested were prepared according to the manufacturers' instructions for use as luting agents. Mean compressive and diametral strengths and standard errors were calculated for each luting agent (n = 10). Analysis of variance was computed (p < 0.0011) and multiple comparisons tests were performed. Compressive strengths varied from 41.5 MPa for a hydroxyapatite ALA to 178.5 MPa for a composite resin ALA. Diametral tensile strengths ranged from 8.1 MPa for a hydroxyapatite ALA to 45.1 MPa for a composite resin ALA. Conventional powder-liquid glass ionomer ALAs, an encapsulated glass ionomer ALA, a composite resin-glass ionomer hybrid ALA, and the composite resin ALAs demonstrated significantly greater compressive and diametral strengths than the zinc phosphate cements.

Analysis of Variance↗

A new rigid biodegradable anchor for meniscus refixation: biomechanical evaluation.

All-inside repair devices have been developed to overcome the disadvantages of conventional suture techniques (such as vein and nerve damage and increased OR time). The Contour Meniscus Arrow is a second generation of the first biodegradable all-inside implant, the Meniscus Arrow. The aim of this study was to compare the biomechanical properties of the Contour Meniscus Arrow to the first-generation Meniscus Arrow and vertical or horizontal suture techniques. In fresh frozen bovine menisci, initial fixation strength, stiffness and failure mode of four different meniscus refixation techniques (Meniscus Arrow, Contour Meniscus Arrow, vertical and horizontal 2-0 Ethibond suture techniques) were evaluated in a computer-based material-testing machine at a rate of 12.5 mm/s. Vertical meniscus sutures showed the highest initial fixation strength, followed by the horizontal suture technique and the Contour Arrow. The Meniscus Arrow showed inferior pull-out strength. Subjecting the different refixation techniques to cyclic testing decreased the fixation strength in all groups. The modified Meniscus Anchor (Contour Arrow) provides biomechanical properties that are superior (pull-out strength) or similar (stiffness) compared to the first biodegradable all-inside implant, the Meniscus Arrow. The pull-out strength of the Contour Arrow was comparable to the pull-out strength reported for horizontal meniscus sutures in the literature. These biomechanical characteristics of this new implant justify clinical use.

Absorbable Implants↗