SEARCH · PubMed Health

Results for “Tibia”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,261 records · Page 70Linked to original sources

PubMed1990

Bone response to hydroxyapatite particles of different shapes in rabbit tibia.

Four holes drilled in rabbit tibia were filled with different commercial hydroxyapatite products. All particles caused a mild inflammatory reaction, which disappeared in 2 months. The line between the new and original bone was visible even after 6 months. No differences in healing pattern were discovered for the various products. When bone cavities are filled with hydroxyapatite particles, the shape of the particles does not seem to affect the healing process.

Animals↗

PubMed1989

Epiphyseal lesions of the femur and tibia in rats following oral chronic administration of zinc dimethyldithiocarbamate (ziram).

A 24-month chronic feeding toxicity study with zinc dimethyldithiocarbamate (ziram) was performed on Fischer 344 rats of both sexes (80 animals/sex per group) at dietary levels of 0, 20, 200, or 2000 ppm. Eight animals of either sex from each group were sacrificed after 26, 52 and 78 weeks, and all surviving animals were killed after 104 weeks. Epiphyseal abnormalities in the long bones of the hind legs were observed in both sexes at 2000 ppm. Clinically, 3 male rats showed partial paralysis of the hind legs. At necropsy, marked curvature of the proximal end of the crus which could cause a restricted extension of the tibio-femoral joint was seen in 11 of 34 males killed at terminal sacrifice. While females had neither clinical signs nor gross abnormalities during the study, histopathological examination revealed retarded epiphyseal closure of the proximal end of the tibia in both sexes. Females also showed the epiphyseal lesion at the distal end of the femur. In severely affected rats marked proliferation of epiphyseal cartilaginous tissue was also noted together with the irregular arrangement of chondrocytes. These changes were evident only in aged animals. The incidence of the lesions in all males and females examined in this group was 22/77 (29%) and 13/73 (18%), respectively. The occurrence of the lesions appeared to be caused by impaired regulation of epiphyseal closure which might be related to the treatment with ziram.

Administration, Oral↗

PubMed1996

The vibrational mode of the tibia and assessment of bone union in experimental fracture healing using the impulse response method.

This study attempts to clarify the use of the impulse response method in the assessment of fracture healing. The vibrational mode as well as the effect of simulated callus consolidation on the vibrational parameters of excised human tibia were studied. Two separate vibrations were found, one vibrating strongly in the lateral direction and the other vibrating weakly in the antero-posterior direction. The ability to identify the primary vibrational mode in the lateral direction would make the impulse response method suitable for use in clinical practice. The callus consolidation process was simulated by the sequential consolidation of an adhesive material in an experimentally produced fracture gap. The change in hardness of the epoxy was found to correlate well with the change of resonant frequency of the bone. The resonant frequency demonstrated a steady increase during the initial phase of consolidation of the adhesive, up to about 40% of its final hardness. With the addition of various constructs for fracture fixation to the in vitro model such as a plate, Ender's pins, a Russell-Taylor intramedullary nail, or an Orthofix external fixator, the relationship between the consolidation of the 'callus' and the change in resonant frequency of the bone was not disturbed.

Bony Callus↗

PubMed1992

A quantitative evaluation of osteoblast-osteocyte relationships on growing endosteal surface of rabbit tibiae.

Scanning electron microscopy (SEM) was used to quantify the intercellular relationships between osteoblasts and osteocytes on the growing endosteal surfaces of the medullary canal of the tibia in four rabbits of different ages. The area of each osteoblast was measured on the SEM micrographs by means of an Image Analyzer. The number of osteocyte cytoplasmic processes was indirectly evaluated by counting the canalicular openings present on the same microscopic fields after the removal of the osteoblasts. The metabolic activity of the osteoblasts was indirectly evaluated from their shape, and the structure was analyzed by transmission electron microscope (TEM) in sections taken from the samples studied by SEM. In all four animals, the surface area of the osteoblasts (OA) was found to vary a great deal, whereas the density of canalicular openings was fairly uniform. Moreover, although the OA mean value increases significantly with the age of the animals, the density of canalicular openings does not; it would therefore appear that the older the animal and the more flattened the osteoblasts, the greater the number of canaliculi beneath them. Since osteoblast activity has previously been shown to be inversely proportional to the area of the protoplasm in contact with the bone surface, it appears that the less active osteoblasts should contact a greater number of osteocyte cytoplasmic processes. These findings suggest that osteocytes might play an important role in modulating osteoblast activity and in recruiting osteoblasts that differentiate into osteocytes, possibly by means of inhibitory signals transmitted via gap junctions.

Animals↗

PubMed2005

Histological findings in titanium implants coated with calcium phosphate ceramics installed in rabbit's tibias.

Oral reconstruction using osteointegrated implants are widely indicated nowadays. The implant bone anchorage is very important for its functional stability. Thus, ceramic biomaterials are widely used as coatings of the implant surfaces to accelerate local osteogenesis. The purpose of this study is to assess the biocompatibility and the osteoconduction of two types of calcium phosphate ceramics used as titanium dental implant coatings. These implants were installed in rabbit tibia during an 8-week healing period. The light and fluorescent microscopy observations showed that the materials are biocompatible and that they have osteoconductive activities.

Animals↗

PubMed2003

Epiphyseal osteoblastoma of tibia with xanthomatous stromal reaction.

Osteoblastoma occurring in long bones has a distinctive predilection for the metaphysis and the diaphysis. Epiphyseal location is rare. Although variation in histologic patterns is a well-known feature of this tumor, xanthomatous stromal reaction has not yet been described. We report a case of a 34-year-old man who developed an osteoblastoma primarily located in the epiphysis of his left tibia with extension into the metaphysis. The striking histologic features included a prominent xanthoma-like stroma consisting of foamy histiocytes in addition to focal areas with classical configuration of an osteoblastoma. The significance of this finding is discussed.

Adult↗

PubMed2006

Total knee arthroplasty using computer-assisted navigation in patients with deformities of the femur and tibia.

Anatomic aberrations of the femur and tibia secondary to trauma, congenital defects, and prior surgery present challenges for the reconstructive knee surgeon because of an altered mechanical axis and distorted anatomic landmarks. Five patients with arthritis of the knee and extra-articular femoral and/or tibial deformity, retained hardware, or intramedullary (IM) implants underwent total knee arthroplasty using a computer navigation system. The navigation system obviated the need for an IM guide, and the normal mechanical axis of the patients was restored. Extensive dissection for hardware removal or osteotomy was not necessary in these patients. In these 5 cases, a navigation system proved to be an effective tool for restoration of limb alignment in the presence of significant extra-articular deformities and/or IM hardware. Thus, it provides an alternative approach to the traditional IM instrumentation for treating these patients in an effective manner.

Aged↗

PubMed2006

A rat model of bone cancer pain induced by intra-tibia inoculation of Walker 256 mammary gland carcinoma cells.

This study described a modified rat model of bone cancer pain. Syngeneic Walker 256 mammary gland carcinoma cells were injected into the tibia medullary cavity via intercondylar eminence. Series of tests were carried out including bone radiology, bone histology, ambulatory pain, thermal hyperalgesia, mechanical allodynia, weight bearing ability, and electrophysiological recording from primary afferent fibers. The rats inoculated with carcinoma cells showed significant ambulatory pain, mechanical allodynia, and reduction in weight bearing, as well as increased incidence of spontaneous activity in Abeta fibers in affected limb, whereas PBS (vehicle) or heat-killed cells (sham) injected rats showed no significant difference in comparison to normal rats. The pain hypersensitive behaviors were aggravated with time and destruction of bone. Interestingly, mechanical allodynia was also observed in the contralateral limb, indicating the involvement of 'mirror image' pain in bone cancer pain. In summary, the present study provided a useful and easily established rat model of bone cancer pain which will contribute to further study of the mechanisms underlying cancer pain.

Action Potentials↗

PubMed2004

Implantation of hydrophilic and hydrophobic titanium discs in rat tibia: cellular reactions on the surfaces during the first 3 weeks in bone.

In a previous study, a method for evaluation of short-time (1-8 days) healing of titanium implants in rat tibiae was described (J. Biomed. Mater. Res. 66A(3) (2003) 662). The implants were disc-shaped and cells and tissue on the surface were investigated, not the adjacent tissue. In this study healing during the first 3 weeks in bone was examined and the healing response between hydrophilic and hydrophobic titanium was compared. Immunofluorescence techniques were used to detect signs of bone formation on the surfaces. Cell viability, alkaline phosphatase (ALP) activity, presence of osteocalcin and cells positive for bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were investigated. Both viable and non-viable cells were found on both surfaces during the first week. Only initially was there a difference between them; 4% viable cells on hydrophilic discs compared to 56% on hydrophobic ones. More BMP-2 positive cells were found on hydrophilic discs than on hydrophobic ones after 1 week. VEGF was detected after 8 days on both surfaces. Osteocalcin positive cells were found from 2 weeks. ALP positive cells were found after 8 days, while at 2-3 weeks ALP positive tissue was abundant on both surfaces. In conclusion, signs of bone formation were detected during the period investigated. Surface energy appeared to be of more importance initially, with higher surface energy resulting in more rapid cell activation and differentiation than lower.

Animals↗

PubMed2004

Detecting and tracking local changes in the tibiae of individual rats: a novel method to analyse longitudinal in vivo micro-CT data.

In this study we present the analysis of in vivo micro-CT scans using a new method based on image registration that accurately evaluates longitudinal micro-CT studies. We tested if detailed changes in the bone architecture could be detected and tracked in individual animals. A prototype in vivo micro-CT scanner (Skyscan 1076) was developed in which tibiae of rats that are lying on a bed under gas anaesthesia were scanned. For this study, three female Wistar rats were used: a sham-operated rat, an ovariectomised (OVX) rat and one rat that served as a reproducibility control. The reproducibility control rat was scanned twice in 1 day. The other animals were scanned at week 0, just before surgery, at week 4 and at week 14 after surgery. Architectural changes over time were detected by overlaying two data sets made at different time points using an algorithm that uses mutual information for optimal registration. The scans were segmented into binary data sets using a local thresholding algorithm. The reproducibility test showed small errors of less than 3% in bone volume measurements and errors less than 0.5% in measurements of trabecular thickness. The sham-operated rat showed no changes in total bone volume, though thinning and eventual loss of some small trabeculae could be detected, which could be related to the age of the animal. The OVX rat lost much trabecular bone volume, especially in the metaphysis (60% at week 4, 75% at week 14). The remaining trabeculae slowly increased in thickness. Following the different scans in time showed the forming of new trabecular structures. Additionally, small longitudinal growth at the growth plate could be detected after the first 4 weeks. Further, the OVX rat showed extensive modelling at the proximal endosteal lateral cortex. We have shown a new method that can detect and track changes in the local bone architecture and individual trabeculae in time, in an individual living animal. This method enables longitudinal in vivo micro-CT studies and has the potential to greatly contribute to experimental rat or mouse studies on pharmacological intervention and transgenic models.

Animals↗

PubMed2004

Immunolocalization of matrix metalloproteinase-13 on bone surface under osteoclasts in rat tibia.

Matrix metalloproteinase (MMP)-13 (an interstitial collagenase also called collagenase 3) is involved in degradation of extracellular matrix in various tissues. Using immunohistochemistry and Western blotting, we investigated localization of MMP-13 in rat tibia, to clarify the role of MMP-13 in bone resorption. MMP-13 reactivity was mainly seen on bone surfaces under osteoclasts, and in some osteocytes and their lacunae near osteoclasts. However, immunoreactivity was not seen in chondrocytes or osteoclasts. MMP-13 was also localized on cement lines in the epiphysis. In the growth plate erosion zone, perivascular cells showed MMP-13 reactivity. Immunoelectron microscopy revealed that MMP-13 was localized on the bone surfaces, under the ruffled borders and some clear zones of osteoclasts. Gold-labeled MMP-13 was closely associated with collagen fibrils. Gold labeling was also detected in Golgi apparatus of osteocytes adjacent to osteoclasts and bone lining cells. Western blotting showed that MMP-13 was mainly associated with mineralized bone matrix. These findings suggest that MMP-13 synthesized and secreted by osteoblast-lineage cells is localized under the ruffled borders of osteoclasts. MMP-13 may play an important role in degradation of type I collagen in bone matrix, acting in concert with cathepsin K and MMP-9 produced by osteoclasts. MMP-13 in perivascular cells may be involved in removal of cartilage matrix proteins such as type II collagen and aggrecan.

Animals↗

PubMed2006

Bisphosphonates suppress periosteal osteoblast activity independently of resorption in rat femur and tibia.

Recent studies demonstrate that bisphosphonates suppress bone resorption by leading to apoptosis of the osteoclast and inhibiting the differentiation to mature osteoclasts. The influence of bisphosphonates on bone formation is unknown, although it has been hypothesized that bisphosphonates inhibit osteoblast apoptosis and stimulate osteoblast proliferation and differentiation in vitro, leading to increased bone formation. The purpose of this study was to investigate the effect of bisphosphonates on bone formation. We administered risedronate at 0.05, 0.5 or 5.0 microg/kg/day or alendronate at 0.1, 1.0 or 10 microg/kg/day subcutaneously for 17 days to 6-month-old female Sprague-Dawley rats. Control rats were given a daily subcutaneous injection of saline. Following sacrifice, the femoral and tibial mid-diaphyses were harvested and mineralizing surface (MS/BS), mineral apposition rate (MAR) and bone formation rate (BFR/BS) were measured on periosteal and endocortical surfaces. In the femur, periosteal MAR was significantly lower in all treatment groups (22-29% for risedronate, 26-36% for alendronate) than in control. In the tibia, periosteal MAR and BFR of all treatment groups were significantly lower (41-50% for risedronate, 43-52% for alendronate) than in the control group. Because the periosteal surfaces of these bones are only undergoing bone formation in modeling mode, our results show that bisphosphonates suppress bone formation independently of bone resorption. Because this effect is seen on periosteal MAR rather than on periosteal MS/BS, we hypothesize that bisphosphonates affect the activity of individual osteoblasts at the cell level. This may help to explain the reason that the anabolic effects of teriparatide are blunted when administered concurrently with or following a course of bisphosphonates in humans.

Alendronate↗

PubMed2006

Sexual dimorphism affects tibia size and shape but not tissue-level mechanical properties.

Understanding how growth influences adult bone morphology and tissue quality should provide important insight into why females show a greater incidence of stress fractures early in life and fragility fractures later in life compared to males. The objective of this study was to test whether females acquire similar tissue-level mechanical properties as males by the time peak bone properties are established. Standardized beams of bone were machined from the tibial diaphyses of 14 young, adult females ranging in age from 22 to 46 years. Data for males (n=17, age=17-46 years) were taken from a prior study. Measures of tissue-level mechanical properties, including stiffness, strength, ductility, toughness, and damageability, were compared between sexes using t-tests. The relationship between cross-sectional morphology and tissue-level mechanical properties was also examined. Males and females showed nearly identical tissue-level mechanical properties. Both sexes also showed similar age-related degradation of mechanical properties and a similar relationship between cross-sectional morphology and tissue quality. However, for all body sizes, female tibiae were smaller relative to body size (i.e., less robust) compared to males. The results indicated that sex-specific growth patterns affected transverse bone size, but did not affect tissue-level mechanical properties. This, combined with the observation that young, adult female long bones are undersized relative to body size, suggests that adult females would be expected to accumulate more damage under intense loading compared to males. This may be a contributing factor to the greater incidence of stress fractures observed for female military recruits.

Adolescent↗

PubMed2005

The effect of cyclic loading on the biomechanical characteristics of the femur-graft-tibia complex after anterior cruciate ligament reconstruction using Bone Mulch screw/WasherLoc fixation.

BACKGROUND: The Bone Mulch screw/WasherLoc fixation system has attracted notice because of its possible advantages. The purpose of the present study was to compare the biomechanical properties of this fixation system for the double-looped flexor tendon graft with those of two standard fixation techniques that had been commonly performed in anterior cruciate ligament reconstruction. METHODS: Anterior cruciate ligament reconstruction was carried out in each group using one of three different procedures (n=14 for each procedure). For each group, seven femur-graft-tibia complexes underwent submaximal cyclic displacement of 5000 cycles after an initial tension of 20N was applied. Then, tensile testing was performed for the complex at a single position, 45 degrees of knee flexion, in which the longitudinal axis of the graft coincided with the axis of the bone tunnels. The remaining seven complexes in each group were examined in the same tensile test without applying any cyclic displacement. FINDINGS: At the 5000th cycle of the displacement, the peak load of the complex with the Bone Mulch screw/WasherLoc system was significantly higher than that with the Endobutton technique (P<0.0001). After 5000 cycles of displacement, the initial stiffness and the linear stiffness of the complex with the Bone Mulch screw/WasherLoc system were significantly higher than those with the double-looped tendon graft and the Endobutton technique (P<0.0001 for both comparisons), while those with the Bone Mulch screw/WasherLoc system were significantly lower than those with the patellar tendon graft with interference screws (initial stiffness: P=0.0004, linear stiffness: P=0.0007). INTERPRETATION: The present study has clarified that the Bone Mulch screw/WasherLoc system provides high stiffness to the complex for the double-looped flexor tendon graft.

Animals↗

PubMedDate not supplied

MRI findings in regional migratory osteoporosis of the knee migrating from the femur to the tibia.

Regional migratory osteoporosis (RMO) is an uncommon self-limiting disease characterized by migrating bone marrow edema and osteoporosis. RMO of the knee with intra-articular migration is very rare. In such cases, different parts of the femur are usually involved. We report a case of intra-articular RMO of the knee migrating from the femur to the tibia--a pattern of migration that has not been previously described in the literature.

Femur↗

PubMed2003

TomoFix: a new LCP-concept for open wedge osteotomy of the medial proximal tibia--early results in 92 cases.

High Tibial Osteotomy (HTO) is an established treatment for unicompartmental osteoarthritis of the knee with malalignment. The classic procedure for correcting varus deformity is the lateral closed wedge osteotomy of the tibia with osteotomy of the fibula. The disadvantages of this technique are well known. Open wedge osteotomy from the medial side eliminates the risk of compartment syndrome and peroneal nerve injuries. A new fixation device (TomoFix) with an adapted surgical technique allows stable fixation of the osteotomy without the need to fill the osteotomy gap with bone grafts. In a prospective study, 92 consecutive cases were treated with this procedure. Bony healing with remodelling of the medial and posterior cortical bone was observed. Full weight-bearing was possible ten weeks after surgery. There were no implant failures. Complications included one delayed union, two revarisations and one deep infection.

Adolescent↗

PubMed2005

Should the tibia be reamed when nailing?

A systematic review was performed to assess reamed and unreamed tibial nailing. Only prospective, randomised studies comparing reamed and unreamed tibial nailing in adults were included. A literature search found 1200 possible articles. There were seven comparative studies. These articles were independently assessed by all three authors. Three studies met the inclusion criteria. Each outcome measure tested was assessed for heterogeneity. If significant heterogeneity was present, data from the studies was not combined. If there was no significant heterogeneity, a combined odds ratio was calculated using a fixed effects model and a Z-test was performed to test the overall effect. Two hundred and ninety-one tibial shaft fractures were entered into the included studies. Two hundred and eighty (96%) were followed up (148 reamed; 132 unreamed). There was an increased non-union rate when the tibia was not reamed (p = 0.02). Screw breakage was more common in the unreamed group (p<0.0001). This study could find little difference in the incidence of other complications following reamed or unreamed tibial nailing.

Adult↗

PubMed2005

Transitional fractures of the distal tibia.

INTRODUCTION: The purpose of the study was to determine the outcome after treatment of transitional fractures of the distal tibia, including growth disturbances, osteoarthritis, and/or restriction of the ability to take part in sports. PATIENTS AND METHODS: In a retrospective multicentre study of 72 patients, 50 were evaluated by clinical and 46 by radiographic examination, an average of 7.4 years (standard deviation 5.9) after treatment. Of these 50 patients, initially 20 were treated non-operatively and 30 were treated operatively. The mechanism was mainly supination trauma. RESULTS: There were four revision operations, including two for fracture displacement after earlier non-operative treatment. No patients reported impairment of sports activities. In no case was the range of movement in the upper ankle joint decreased by >10 degrees . Radiographic examination revealed small osteophytes in one case and narrowing of the joint space in two; there was no axial deviation or clinical problems in any of these cases. There were no significant differences between the group treated non-operatively and the group that underwent surgery. The intermediate- to long-term outcome presented was good or very good in both the surgically and conservatively treated patients. Only two of the initially nondisplaced fractures later became displaced. This finding emphasises that operative treatment is not indicated for all nondisplaced fractures. Axial deviations may not occur, because of the little growth potential of the partially closed physis.

Adolescent↗