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Gravitational evolution of a perturbed lattice and its fluid limit.

We apply a simple linearization, well known in solid state physics, to approximate the evolution at early times of cosmological N-body simulations of gravity. In the limit that the initial perturbations, applied to an infinite perfect lattice, are at wavelengths much greater than the lattice spacing l, the evolution is exactly that of a pressureless self-gravitating fluid treated in the analogous (Lagrangian) linearization, with the Zeldovich approximation as a subclass of asymptotic solutions. Our less restricted approximation allows one to trace the evolution of the discrete distribution until the time when particles approach one another (i.e., "shell crossing"). We calculate modifications of the fluid evolution, explicitly dependent on l, i.e., discreteness effects in the N-body simulations. We note that these effects become increasingly important as the initial redshift is increased at fixed l.

Journal Article↗

Oxygen evolution and the permeability of the outer envelope of isolated whole chloroplasts.

A rapid oxygraph method of studying the permeability of the envelope of isolated chloroplasts was used. The outer envelope of aqueously isolated whole spinach (Spinacia oleracea L.) chloroplasts in buffer is readily permeable to 3-phosphoglyceric acid, which induces an immediate light dependent oxygen evolution. This light dependent oxygen evolution was completely eliminated by swelling these plastids in an osmotically dilute solution. Exogenous adenosine diphosphate, but not inorganic phosphate, strongly stimulated this oxygen evolution. This indicated that the chloroplast envelope is relatively permeable to adenosine diphosphate.Oxygen evolution and swelling studies indicated that the chloroplast envelope is relatively impermeable to NADP and to ferredoxin.A method is described whereby the percent of whole chloroplasts present in a chloroplast preparation may be rapidly estimated.

Journal Article↗

Glycolate stimulation of oxygen evolution during photosynthesis.

Glycolate and glyoxylate stimulated 100% to 300% the rate of oxygen evolution by Scenedesmus in the light in the absence of added carbon dioxide. This stimulation occurred either aerobically or anaerobically, and was sensitive to CMU. Aerobic dark respiration was stimulated 25% to 100% by glycolate. This phenomenon was best demonstrated with synchronized Scenedesmus at the stage of cell division. For glycolate stimulation of oxygen evolution, a dark preincubation of 1 minute or less was necessary. In comparative test with other compounds of metabolism and photosynthesis, the stimulation of oxygen evolution was greatest by glycolate and glyoxylate. In a proposed scheme glyoxylate serves as a terminal hydrogen acceptor from NADPH produced by photosynthesis, and it thereby stimulates oxygen evolution when carbon dioxide is not available. Transformation of glycolate to glyoxylate in these cells would have to occur in the absence of oxygen.

Eukaryota↗

Inhibition of oxygen evolution in chloroplasts isolated from leaves with low water potentials.

Chloroplasts were isolated from pea and sunflower leaves having various water potentials. Oxygen evolution by the chloroplasts was measured under identical conditions for all treatments with saturating light and with dichloroindophenol as oxidant. Evolution was inhibited when leaf water potentials were below -12 bars in pea and -8 bars in sunflower and the inhibition was proportional to leaf water potential below these limits. Inhibition was more severe in sunflower than in pea chloroplasts. In sunflower, it could be detected after 5 minutes of leaf desiccation, and, up to 1 hour, the effect was independent of the duration of low leaf water potential.In high light, the reduction in activity of sunflower chloroplasts paralleled the reduction in CO(2) fixation by intact sunflower plants having low leaf water potentials. Stomatal apertures and transpiration rates were also reduced under these conditions and were probably limiting. In low light, intact sunflowers required more light per unit of CO(2) fixed when leaf water potentials were low than when they were high. This increased light requirement in the intact system was of a magnitude which could be predicted from the reduced oxygen evolution by the isolated chloroplasts. It was concluded that moderately low leaf water potential affects photosynthesis in at least two ways: first, through an inhibition of oxygen evolution by chloroplasts and, second, by closure of stomata in intact leaves.

Journal Article↗

Relationship between Ethylene Evolution and Senescence in Morning-Glory Flower Tissue.

An excised tissue system consisting of corolla rib segments was developed to study the relationship between senescence and ethylene production in morning-glory flowers (Ipomoea tricolor). Such segments, isolated 1 or 2 days (day -1 or day -2) before flower opening (day 0) passed through the same developmental phases as did the corresponding tissues of the intact organ. When excised on day -1 and incubated overnight, the rib segments turned from purple to blue and changed from a slightly curled to a flat configuration. On day 0, these segments rolled up during the afternoon and turned purple again, as did the ribs of an intact corolla; the rolling up coincided with an increased rate of ethylene production. Premature rolling up and associated ethylene evolution were induced by ethylene or propylene treatment. When segments were excised on day -2 and incubated overnight, there were no changes in color or shape; during day -1, no spontaneous rolling up and little ethylene evolution occurred. Application of ethylene or propylene to these immature segments elicited rolling up but did not stimulate endogenous ethylene production.Overnight treatment of segments cut on day -1 with 10(-6)m benzyladenine markedly retarded spontaneous rolling up and ethylene evolution, although the response to applied ethylene was only slightly slowed. Overnight treatment of segments cut on day -1 with the ethoxy analog of rhizobitoxine (10(-5) to 10(-4)m) resulted in almost complete (>99%) inhibition of both spontaneous and propylene-induced ethylene evolution. Although spontaneous rolling up was delayed, it was not abolished, and ethylene-induced rolling up was almost unaffected.These data indicate that an ethylene-generating system develops as an integral part of the aging process in flower tissue. Ethylene hastens aging of the flower, but may not play an obligatory role in flower senescence.

Journal Article↗

Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: V. Effect of Abscisic Acid on Indoleacetic Acid Metabolism and Ethylene Evolution.

The wilty tomato mutant flacca, the normal cultivar Lycopersicon esculentum Mill. Rheinlands Ruhm, and abscisic acid-induced phenotypic revertants were compared with respect to ethylene evolution, activity of tryptophan aminotransferase, and [1-(14)C]indoleacetic acid decarboxylation.The level of ethylene evolution was higher in flacca plants than in the normal cultivar. Ethylene evolution was reduced to the wild type level in abscisic acid-induced phenotypic revertants and to a lesser extent in mutant plants grown under humid conditions. Leaf epinasty, which characterized flacca plants in the present experiments, did not appear in absciscic acid-treated mutant plants, but did appear under high humidity. Tryptophan aminotransferase activity, similar to ethylene evolution, was higher in flacca plants and was reduced to the normal level by abscisic acid treatment. Indoleacetic acid decarboxylation was similar in mutant and normal plants, but was increased by abscisic acid treatment. The relationships among ethylene, auxin, and the morphological symptoms which characterize the mutant are discussed.

Journal Article↗

Significance of hydrogen evolution in the carbon and nitrogen economy of nodulated cowpea.

The carbon and nitrogen economies of a single cultivar of cowpea (Vigna unguiculata (L.) Walp.cv Caloona) nodulated with either a high H(2)-evolving strain (176A27) or a low H(2)-evolving strain (CB756) of Rhizobium were compared. The two symbioses did not differ in total dry matter production, seed yield, nitrogen fixed, the spectrum of nitrogenous solutes produced by nodules for export, or the partitioning of net photosynthate within the plant throughout the growth cycle. Detailed examination of the carbon and nitrogen economy of the nodules, however, showed a significant difference between the symbioses. Nodules formed with CB756 lost less CO(2) in respiration compared to the higher H(2)-evolving symbioses and this could have been largely responsible for a 36% better economy of carbon use in CB756 nodules during the period of maximum H(2) evolution (48-76 days) and over the whole growth period (20-90 days), a 16% economy. In terms of overall net photosynthate generated by the plant, these economies were equivalent to 5% and 2% of the carbon utilized in the two periods, respectively. From the differences in H(2) evolution and CO(2) production by nodules of the two symbioses, the cost of H(2) evolution was found to be 3.83+/-0.6 millimoles CO(2)/millimoles H(2) for plants grown in sand culture and 1.69 +/- 0.48 millimoles CO(2)/millimoles H(2) for those in water culture. In both symbioses, the ratio of H(2) evolution to N(2) fixed varied markedly during ontogeny, indicating a significant variation in the relative efficiency and thus metabolic cost of N(2) fixation at different stages during development.

Journal Article↗

d-Glycerate Transport by the Pea Chloroplast Glycolate Carrier : Studies on [1-C]d-Glycerate Uptake and d-Glycerate Dependent O(2) Evolution.

The transport of glycolate and d-glycerate, the substrate and end product of the photorespiratory carbon pathway, respectively, by isolated intact pea (Pisum sativum) chloroplasts has been compared. d-Glycerate uptake was inhibited by the 2-hydroxymonocarboxylates, glycolate, glyoxylate, and d-lactate. Phosphate and phosphoglycerate and triose phosphates were without effect when the assays were carried out for two seconds. Glycolate was found to be a competitive inhibitor of d-glycerate uptake and the presence of glycolate in the chloroplast stroma strongly enhanced d-glycerate uptake from the medium. For optimal rates of d-glycerate-dependent O(2) evolution by pea chloroplasts, phosphate, ADP or ATP, and a mediator of cyclic electron flow had to be added. The inhibition of d-glycerate-dependent O(2) evolution by triose phosphates and 2-hydroxymonocarboxylates was tested. The inhibition of d-glycerate-dependent O(2) evolution by these metabolites did not correlate with their effects on glycerate transport. Thus, metabolism of d-glycerate, rather than its transport, limits the rate of glycerate-dependent oxygen evolution. The ramifications of d-glycerate metabolism on the interpretation of d-glycerate uptake data obtained with prolonged incubations are discussed. We conclude that d-glycerate and glycolate transport are mediated by the same transport system.

Journal Article↗

Alternative respiration and heat evolution in plants.

The alternative respiratory pathway dissipates most of the chemical energy of respiratory substrates as heat. We have shown that this heat can be quantified by microcalorimetry and is a measure of alternative pathway activity in vivo. The alternative pathway is known to increase in aged potato (Solanum tuberosum) slices and in chill-stressed leaves. Aging of potato slices for 24 hours was accompanied by an almost fourfold increase in the rate of heat evolution. This heat increase was resistant to KCN but could be blocked by an alternative pathway inhibitor, salicylhydroxamic acid (SHAM). In cucumber (Cucumis sativus) leaves subjected to chilling stress (between 4 and 16 degrees C), the rate of heat evolution was inversely related to temperature. As in aged potato slices, the increased rate of heat evolution in cucumber leaves was blocked by SHAM, but not by KCN. Nitrogen or the combination of SHAM and KCN blocked most of the heat evolution in both aged potato slices and chill-stressed cucumber leaves. Calorimetric measurements of the alternative pathway corresponded to respiration measurements performed using an oxygen electrode.

Journal Article↗

The evolution strategy--a search strategy used in individual optimization of electrical parameters for therapeutic carotid sinus nerve stimulation.

Optimization problems, arising in the search for parameters and/or techniques of functional electrostimulation (FES), disproportionally increase when multiple electrodes, electrode configurations, electrical parameters, and stimulation modes may be applied. When computational or investigational effort precludes systematic studies in FES, we propose to apply and evaluate Rechenberg's evolution strategy, which in technical use and numerical optimization has been valid in comparison to more traditional methods. This strategy implements mutation and selection processes in analogy to biological evolution. The effect of combined multiple input variables on a quality function (Q) is experimentally evaluated. The actual computed value of Q serves as a selection criterion for those input variable combinations which lead Q to approach a target value (maximization), similar to a hill-climbing procedure. In radiofrequency controlled, therapeutic electrical carotid sinus nerve stimulation (CSNS), we varied (mutated) combinations of pulse frequency and pulse amplitude parameters, according to the evolution strategy, in individual patients. CSNS lowers blood pressure and decreases heart rate. Q was computed from blood pressure and heart rate responses to CSNS. The strategy individually optimized electrical parameters to achieve large depressor responses upon CSNS. Although, in contrast to technical usage, only two input variables were investigated, and biomedical experience with the evolution strategy is limited so far, its potential use in other fields of FES, especially when more input variables are to be optimized, is discussed and encouraged.

Algorithms↗

The evolution of alternative cryptic female choice strategies in age-structured populations.

Cryptic female choice is a potentially important aspect of the sexual selection process. According to the theory of sexual dialectics, postcopulation manipulation of relative male fertilization success can provide an avenue by which females can circumvent attempts by males to control female reproduction. Here I use stochastic models to investigate the evolution of cryptic female choice in populations with and without age structure. In populations without age structure, cryptic female choice will evolve only when (1) precopulatory mate choice by females is inefficient, (2) variation in male fitness is correlated with a trait upon which a female can base her choice of mates, and (3) the cost of multiple mating is not too high. In populations with age structure, similar conditions apply. However, selection sometimes favors females that employ alternative strategies of female choice at different ages. These results help to define the types of biological systems in which we should expect to see the evolution of cryptic female choice. They also illustrate that the evolution of choice strategies in females may be complex and may mirror in some important respects the evolution of alternative mating tactics in males.

Age Factors↗

Arboreallty and morphological evolution in ground beetles (Carabidae: Harpalinae): testing the taxon pulse model.

One-third to two-thirds of all tropical carabids, or ground beetles, are arboreal, and evolution of arboreality has been proposed to be a dead end in this group. Many arboreal carabids have unusual morphological features that have been proposed to be adaptations for life on vegetation, including large, hemispheric eyes; an elongated prothorax; long elytra; long legs; bilobed fourth tarsomeres; adhesive setae on tarsi; and pectinate claws. However, correlations between these features and arboreality have not been rigorously tested previously. I examined the evolution of arboreality and morphological features often associated with this habitat in a phylogenetic context. The number and rates of origins and losses of arboreality in carabids in the subfamily Harpalinae were inferred with parsimony and maximum-likelihood on a variety of phylogenetic hypotheses. Correlated evolution in arboreality and morphological characters was tested with concentrated changes tests, maximum-likelihood, and independent contrasts on optimal phylogenies. There is strong evidence that both arboreality and the morphological features examined originated multiple times and can be reversed, and in no case could the hypothesis of equal rates of gains and losses be rejected. Several features are associated with arboreality: adhesive setae on the tarsi, bilobed tarsomeres, and possibly pectinate claws and an elongated prothorax. Bulgy eyes, long legs, and long elytra were not correlated with arboreality and are probably not arboreal adaptations. The evolution of arboreal carabids has not been unidirectional. These beetles have experienced multiple gains and losses of arboreality and the morphological characters commonly associated with the arboreal habitat. The evolutionary process of unidirectional character change may not be as widespread as previously thought and reversal from specialized lifestyles or habitats may be common.

Adaptation, Biological↗

Myelodysplastic syndromes: evolution of overt leukaemia by one or several steps of transformation.

The evolution of leukaemia was studied prospectively in 29 patients with myelodysplastic syndrome (MDS) followed for 2-6 years by sequential blast counts, cell kinetics derived from quantitative 14C-autoradiography and karyotype analysis. Overt leukaemia developed in seven patients. Two distinct patterns of leukaemic evolution were identified. The first was characterized by a gradual increase in blast cell count and in the frequency of labelled blasts, and a corresponding reduction in myeloid maturation index indicating increased intracompartmental myeloblast divisions and premature myeloid cell death. A second pattern of leukaemic evolution was marked by a sudden rise in the blast cell population in a previously stable MDS. This rise was attributed both to an increased rate of blast proliferation, and the accumulation of non-proliferating blasts. In an additional patient with smouldering ANLL and multiple karyotype abnormalities, transient clinical remission took place following prednisone and oxymetholone therapy, characterized by a sideroblastic morphology, normal karyotype, and persistence of a highly abnormal myeloid maturation index. The sudden emergence of overt leukaemia in previously stable MDS in some of our patients and the temporary reversal of overt leukaemia into sideroblastic anaemia in one case, lend support to the notion of leukaemic evolution by several steps of transformation. On the other hand, the gradual transition of MDS into overt leukaemia in other patients is compatible with a single step leukaemia transformation, although the possibility of clonal disease prior to the development of MDS cannot be excluded with certainty.

Aged↗

Assessment of clonal evolution at Ig/TCR loci in acute lymphoblastic leukaemia by single-strand conformation polymorphism studies and highly resolutive PCR derived methods: implication for a general strategy of minimal residual disease detection.

Junctional sequences of immunoglobulin (Ig)/T-cell receptor (TCR) gene rearrangements are used as patient-specific PCR targets for the detection of minimal residual disease (MRD) in acute lymphoblastic leukaemias (ALLs). Clonal evolution of gene rearrangements is a major pitfall of this strategy. Using high-resolution PCR-based analyses (including denaturing gel electrophoresis and single-stranded conformation polymorphism (SSCP)) we have compared Ig/TCR gene rearrangements at presentation and relapse in a series of ALLs. These methods allow an unambigous comparison of rearrangements taking into account junctional size and nucleotide sequence information and allow a precise assessment of the clonal evolution. V gamma-J gamma and V delta 1-J delta 1 rearrangements were analysed in 12 T-ALLs. VH-JH, V gamma-J gamma, V delta 2-D delta 3 and, in selected cases, DH-JH rearrangements were studied in 14 B-lineage ALLs. Clonal evolution, regarding major rearrangements, occurs for at least one of these loci in 2/12 T-ALLs and in 5/14 B-lineage ALLs. Clonal evolution is more marked for minor rearrangements than for major ones. As shown using SSCP analysis, rearrangements observed at relapse are sometimes found in minor clones at presentation which are therefore selected in vivo by a proliferative advantage. These data, as well as those from the available literature, suggest the use of at least two patient-specific probes to detect MRD in ALLs. A general strategy including selected Ig/TCR rearrangements and chromosomal abnormalities as PCR targets is proposed.

Adolescent↗

The influence of territoriality and mating system on the evolution of male care: a phylogenetic study on fish.

Evolution of male care is still poorly understood. Using phylogenetically matched-pairs comparisons we tested for effects of territoriality and mating system on male care evolution in fish. All origins of male care were found in pair-spawning species (with or without additional males such as sneakers) and none were found in group-spawning species. However, excluding group spawners, male care originated equally often in pair-spawning species with additional males as in strict pair-spawning species. Evolution of male care was also significantly related to territoriality. Yet, most pair-spawning taxa with male care are also territorial, making their relative influence difficult to separate. Furthermore, territoriality also occurs in group-spawning species. Hence, territoriality is not sufficient for male care to evolve. Rather, we argue that it is the combination of territoriality and pair spawning with sequential polygyny that favours the evolution of male care, and we discuss our results in relation to paternity assurance and sexual selection.

Animals↗

Atypical evolutions of benign localization-related epilepsies in children: are they predictable?

PURPOSE: To emphasize that, in some patients, different atypical evolutions occur in the course of so-called benign focal epilepsies of childhood (BFEC) and to promote interest in finding clinical and/or electroencephalographic (EEG) clues to which patients might be prone to these risky evolutions. METHODS: Twenty-six patients who started with the typical clinical and EEG features of benign childhood epilepsy with centrotemporal spikes (BCECTS) but who had reversible or persistent, serious epileptic events including status epilepticus and language, cognitive, or behavioral impairments were followed for </=14 years. Repeated neurologic examinations, EEG records, and neuropsychological evaluations were done, and brain-imaging studies [computerized axial tomography (CAT) or magnetic resonance imaging (MRI)] were obtained in all patients. RESULTS: The 26 patients were in four separate groups according to the nature of their atypical evolution. Eleven children had atypical benign focal epilepsy of childhood (ABFEC), three with Landau-Kleffner syndrome (LKS), seven with status epilepticus of BCECTS, and five with mixed features of the other three groups. All the children whose BCECTS evolved into ABFEC have finally recovered and are attending normal schools, although five have learning difficulties. Two of the three patients diagnosed with LKS recovered from aphasia, although some language difficulties persist in one. The seven who showed status epilepticus of BCECTS are now normal after 3-14 years of follow-up, and three of the five children showing mixed features fulfilled the criteria for a diagnosis of epilepsy with continuous spikes and waves during slow sleep (CSWS). CONCLUSIONS: A small proportion of cases starting with BCECTS evolve into ABFEC, LKS, status of BCECTS, or epilepsy with CSWS. In such cases, BCECTS is not always benign. Clinical and EEG markers should be sought to predict these atypical evolutions.

Adolescent↗

Mathematical model of gas bubble evolution in a straight tube.

Deep sea divers suffer from decompression sickness (DCS) when their rate of ascent to the surface is too rapid. When the ambient pressure drops, inert gas bubbles may form in blood vessels and tissues. The evolution of a gas bubble in a rigid tube filled with slowly moving fluid, intended to simulate a bubble in a blood vessel, is studied by solving a coupled system of fluid-flow and gas transport equations. The governing equations for the fluid motion are solved using two techniques: an analytical method appropriate for small nondeformable spherical bubbles, and the boundary element method for deformable bubbles of arbitrary size, given an applied steady flow rate. A steady convection-diffusion equation is then solved numerically to determine the concentration of gas. The bubble volume, or equivalently the gas mass inside the bubble for a constant bubble pressure, is adjusted over time according to the mass flux at the bubble surface. Using a quasi-steady approximation, the evolution of a gas bubble in a tube is obtained. Results show that convection increases the gas pressure gradient at the bubble surface, hence increasing the rate of bubble evolution. Comparing with the result for a single gas bubble in an infinite tissue, the rate of evolution in a tube is approximately twice as fast. Surface tension is also shown to have a significant effect. These findings may have important implications for our understanding of the mechanisms of inert gas bubbles in the circulation underlying decompression sickness.

Atmospheric Pressure↗

Testing of some assumptions about biodegradability in soil as measured by carbon dioxide evolution.

Conversion to CO2 upon incubation in aerobic soil is one of the standard test procedures to assess biodegradability. It may be measured with unlabeled test compounds in biometer flasks. In this case, the background CO2 evolution by unamended soil is subtracted from the CO2 evolution by the amended soil and the resulting net CO2 evolution becomes the measure of biodegradation. Alternately, 14CO2 release from radiocarbon substrates is measured to assess biodegradability. Both approaches measure ultimate (complete) biodegradation and bypass the theoretical and technical limitations of residue analysis. This report examines the underlying assumptions that, except for carbon content, conversion percentage to CO2 is relatively independent of chemical composition, that CO2 production is proportional to the amount of added test compound, and that the background CO2 evolution of the soil is not influenced by the test substance. Work with unlabeled and radiolabeled substrates proved the first two assumptions to be essentially correct. However, more than half of net CO2 production may represent the mineralization of biomass and soil organic matter, some of it unrelated to the test compound. The soil microbial community in its nongrowing steady state appears to convert a much lower percentage of a radiocarbon substrate to 14CO2 than a growing soil community that responds to a substantial substrate addition. These findings may help to improve test methods and may aid in the interpretation of test results.

Adipates↗