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PubMedDate not supplied

Changes in the palmar sweat index during mental arithmetic.

This study investigated the influence of activation on the number of active sweat glands (palmar sweat index, PSI), as assessed by the plastic impression method. Subjects were 50 male students, assigned to two groups. The experiment involved 5 phases: an initial adaptation for 6 min in both groups (Phase I), 3 relaxation periods for 10 min (Phases II, IV and V in Group 1, Phases II, III and V in Group 2), and 10 min of mental arithmetic (Phase III in Group 1, Phase IV in Group 2). Prints were taken every 2.5 min from the left middle and forefinger, and skin conductance level (SCL), number of spontaneous fluctuations (SF) and heart rate (HR) were recorded for the corresponding intervals. PSI rose significantly during activation conditions and decreased afterwards. Accordingly, during Phase III values were higher in Group 1, whereas during Phase IV they were higher in Group 2. Decrease of PSI during the first measurements indicated that subjects probably react to the assessment procedure itself and that interpretable baseline values can only be obtained after several initial assessments. Correlations between PSI and electrodermal variables proved to be high within subjects, whereas correlations across subjects were lower.

Adult↗

PubMed1989

Radioimmunoassay detection limits for 19-OH F1 alpha/F2 alpha prostaglandin in normal, infertile and vasectomized semen stains. Analysis of saliva, sweat and urine for possible non-specific or matrix effects.

The sensitivity of a simple radioimmunoassay (RIA) for the detection of 19-OH prostaglandin F1 alpha/F2 alpha (PG F) has been evaluated on a number of semen samples from vasectomized, infertile and fertile donors. The specificity of the test has been examined by testing saliva, sweat and urine from a number of male and female donors. The assay technique could readily detect the PG in semen stains prepared from 0.2 microliter of semen from normal, infertile and vasectomized donors. The detection limit of the assay system, based on the observed displacement, was calculated to be approximately 0.05 microliter semen. The assay could be conducted over a pH range of 7.5-10.5 even after the PG has been heated to 100 degrees C. The 19-OH series of PG were absent from sweat, saliva and female urine using the normal assay protocol; volumes in excess of 100 microliters of some urines particularly from women in labour and those with acute urinary tract infection showed some displacement. Low levels of PG were detected in 50% of the male urine analysed. However, urine samples from men who had engaged in recent sexual activity contained relatively high concentrations of PG which could be readily detected in 10 microliters of urine. These results emphasise the potential of these compounds as specific and sensitive markers for the presence of human semen.

Dinoprost↗

PubMed1991

An outward-rectifying potassium channel in primary cultures of sweat glands from cystic fibrosis subjects.

We have previously described a high conductance calcium-activated 'maxi K' channel in primary cultures of human eccrine sweat gland cells both from normal subjects and those with cystic fibrosis. In further studies we have now identified a potassium-selective channel of much lower conductance which shows outward-rectification and which is present in sweat glands isolated from cystic fibrosis subjects. In experiments with inside-out patches using symmetrical pipette and bath solutions containing 140 mM K+ the channel showed an outward slope conductance (at +50 mV) of approximately 26 pS and an inward conductance (at -50 mV) of approximately 11 pS. When K+ in the bath was replaced by Na+ the reversal potential shifts to reveal a permeability ratio PK/PNa approximately 40 Unlike the maxi-K+ channel, the outward-rectifying channel does not show sensitivity to Ca2+. Channels were found in cells cultured from the glands of four out of five cystic fibrosis subjects. In cells cultured from 30 subjects who did not have cystic fibrosis, an outward-rectifying potassium channel was seen in only one out of approximately 3000 patches.

Calcium↗

PubMed2005

Botulinum toxin A (Botox) and sweating-dose efficacy and comparison to other BoNT preparations.

BACKGROUND: Botulinum toxin type A (BoNT/A) is 20-50 times more effective than Botulinum toxin type B (BoNT/B) concerning the treatment of muscular hypercontractions [Sloop, R.R., Cole, B.A., Escutin, R.O., 1997. Human response to botulinum toxin injection: type B compared with type A. Neurology 49, 189-194]. Botulinum toxins block motor nerves as well as autonomic fibres [Rand, M.J., Whaler, B.C., 1965. Impairment of sympathetic transmission by botulinum toxin. Nature 206, 588-591]. OBJECTIVE: Purpose of this study was to analyse the dose dependent reduction of sweating using the BoNT/A preparation Botox and to compare the results with our earlier results analysing Dysport [Braune, C., Erbguth, F., Birklein, F., 2001. Dose thresholds and duration of the local anhidrotic effect of botulinum toxin injections: measured by sudometry. Br. J. Dermatol. 144, 111-117] and Neurobloc (BoNT/B) [Birklein, F., Eisenbarth, G., Erbguth, F., Winterholler, M., 2003. Botulinum toxin type B blocks sudomotor function effectively: a 6 month follow up. J. Invest. Dermatol. 121, 1312-1316]. METHODS: Different doses of Botox were injected subcutaneously (n=27 healthy subjects). Planimetrical analyses of the area of anhidrosis and quantitative sudomotor-axon-reflex testing (QSART) were done after 3 weeks, 3 and 6 months. RESULTS: A threshold dose of 1.25 MU Botox led to anhidrotic skin spots after 3 weeks. The duration of anhidrosis was prolonged for 3 months when 17.5 MU and for 6 months when 50 MU were injected. Anhidrotic area size decreased with time (p=0.001), indicating partial recovery at the edges. After 3 weeks, QSART had significantly decreased to 29% of baseline. With doses of 70 MU or more it decreased to zero. After 3 months, QSART had returned to 68% of baseline and after 6 months to 87%. CONCLUSIONS: Botox dose-dependently suppressed sweating. Comparison to Dysport and Neurobloc revealed a strikingly similar efficacy after 3 weeks and 3 months for all preparations. BoNT/A in general induced a more sustained anhidrosis than BoNT/B.

Adult↗

PubMed1996

Clonidine in the management of asymmetrical gustatory facial sweating: an N-of-1 trial.

OBJECTIVE: To determine the efficacy of clonidine in the management of gustatory facial sweating (GFS) ipsilateral to an upper thoracic sympathectomy. DESIGN: Placebo-controlled N-of-1 trial. SETTING: Tertiary care referral center. INTERVENTION: Clonidine patch that delivers 0.2mg/d. OUTCOME MEASURE: Daily episodes of GFS are recorded. The severity of GFS is graded as mild, moderate, or severe, and a daily GFS score is calculated. RESULTS: Clonidine was shown to significantly decrease (p < .05) the incidence and frequency of gustatory facial sweating.

Administration, Cutaneous↗

PubMed1991

CFTR: development of high- affinity antibodies and localization in sweat gland.

Rabbit antisera were raised to six synthetic peptides corresponding to amino acid sequences contained in the protein product of the cystic fibrosis gene, CFTR. For two peptides, [Lys102]CFTR(102-116) and CFTR(1468-1480), antibody-peptide binding was of high affinity in that half-maximal binding occurred at peptide concentrations below 10 nM. Monospecific antibodies were prepared using these peptides, and these antibodies were used to stain human skin. Specific staining was detected in the cells lining the reabsorptive duct of the sweat gland. Within these lumenal cells, staining was most prominent at the apical domain but was also detected near the basolateral surface. This finding agrees well with predictions based on the effects of cystic fibrosis on sweat gland function, and suggests that these antibodies will be useful for studying CFTR in other human tissues.

Amino Acid Sequence↗

PubMed2000

Nucleotide-evoked ion transport and [Ca(2+)](i) changes in normal and hyperhidrotic human sweat gland cells.

Apical and basolateral application of ATP and UTP evoked [Ca(2+)](i) and short circuit current (Isc) increases in normal and hyperhidrotic human eccrine sweat gland cells grown into functionally polarised epithelia on permeable supports. Basolateral application to hyperhidrotic cells exhibited a markedly greater increase in Isc than in normal cells. Hyperhidrotic cells also demonstrated differences from the normal in [Ca(2+)](i) and Isc responses to ATP when pre-treated with thapsigargin. The data demonstrate the presence of apical and basolateral receptors that allow nucleotides to increase [Ca(2+)](i) and Isc. The results suggest that changes from the normal in transepithelial ion transport contribute to the characteristic excessive fluid production of hyperhidrotic sweat glands.

Adenosine Triphosphate↗

PubMed1977

Analysis of the proteins in sweat and urine by agarose-gel isotachophoresis.

A sensitive and practical method is described for the analysis of the proteins contained in human sweat and urine which does not require pre-concentration of the sample. Technical details are provided of the agarose-gel isotachophoresis and the proteinograms of normal and pathological urine samples, as well as proteinograms of human sweat. The method can also be applied as an electro-concentration system in a field-strength gradient. By means of this electro-concentration system, and in combination with immunodiffusion against monospecific antisera, a detection limit of albumin of 50 ng/ml has been obtained.

Albumins↗

PubMed1984

Early diagnosis of cystic fibrosis by means of sweat microosmometry.

A modified sweat test procedure was studied to evaluate its practicability in the diagnosis of cystic fibrosis in newborn infants. By means of vapor pressure osmometry, we measured sweat osmolality in 131 healthy children and 63 patients with CF. Reference values were determined according to age: healthy infants 4 to 9 days of age; 121 +/- 23 mmol/kg (mean +/- SD); healthy infants 10 days to 2 months, 109 +/- 13 mmol/kg; healthy infants 2 to 12 months, 86 +/- 13 mmol/kg; and healthy children and adults 2 to 23 years, 105 +/- 14 mmol/kg. The mean of corresponding values for the 63 patients with CF was 267 +/- 34 mmol/kg. There was no overlap of values in any age group, even when the 2 SD limit was taken into account. Thus the diagnosis of CF can be made by means of this approach in newborn infants 8 days of age or older.

Adolescent↗

PubMed1985

Environmental deprivation and transient elevation of sweat electrolytes.

We report five children who had transient elevations in sweat electrolyte values in association with environmental deprivation. The high electrolyte values were not always associated with malnutrition, and normalized more rapidly than weight. The reason for these abnormalities is unknown. In as much as elevated sweat electrolytes are rarely associated with environmental deprivation, repeat studies are recommended before diagnosing cystic fibrosis in such children.

Child, Preschool↗

PubMed1989

An analysis on the rates and regulation of insensible water loss through the eccrine sweat glands.

An analysis is presented on insensible water loss from the human body at rest through exposed skin surfaces into still air. Possible sites of moisture release are identified as the stratum corneum of the skin, free surfaces of dilute sweat liquids perpetually present in the microscopic ducts of a large population of eccrine sweat glands, and moist microvillous processes which line part of the periductal surfaces in the glands, particularly in the helical coils within the stratum spinosum of the epidermis. Water supply to the sites involves transepidermal migration across skin tissue layers, secretion and partial reabsorption of solutes and water within eccrine glands, and transport across periductal lining of eccrine glands from the surrounding connective tissues respectively. Evaporation and gas phase diffusion within eccrine ducts were modelled. Basal loss rates of water (as regulated by the ambient temperature and relative humidity and by aspects of the anatomy of and physiological factors for eccrine glands, the epidermis and the dermis) were calculated at between 1 and 20 g hr-1 at an ambient temperature of 25 degrees C and a relative humidity of 60% as an example. Such rates are significant fractions of experimental values for insensible water loss rates reported at between 4 and 35 g hr-1 in air at 22-30 degrees C and a relative humidity of 30-60%.

Eccrine Glands↗

PubMed1999

Development of muscarinic receptors and regulation of secretory responsiveness in rodent sweat glands.

Sweat glands are innervated by sympathetic neurons which undergo a change in transmitter phenotype from noradrenergic to cholinergic during development. As soon as the glands begin to differentiate, M3 muscarinic receptor mRNA and binding sites are detectable. Receptor expression appears in the absence of innervation and is maintained after denervation. While receptor expression is not regulated by innervation, secretory responsiveness is. Muscarinic blockade during development or in adult animals results in the loss of responsiveness and its reappearance requires several days. Cholinergic muscarinic activation is most likely to regulate one or more steps in the signalling cascade that are downstream of calcium mobilization. The anterograde regulation of sweat gland responsiveness is one facet of the reciprocal interactions are required to establish a functional synapse in this system.

Acetylcholine↗

PubMed1988

Electron microscopic study on the innervation of the human axillary sweat glands.

The innervation of human axillary sweat glands was studied at electron microscopic level. Nerve endings with many small agranular vesicles and few large dense-cored vesicles were found outside the basement membrane of the secretory coils. These nerve endings can be classified as cholinergic. This result confirms the previous knowledge that axillary sweat glands are innervated by sympathetic cholinergic nerves.

Axilla↗

PubMed2002

Histiocytoid variant of eccrine sweat gland carcinoma of the eyelid and orbit: report of five cases.

OBJECTIVE: To study the clinicopathologic features of the histiocytoid variant of adenocarcinoma of the eccrine sweat gland of the eye and orbit. DESIGN: Retrospective case series. PARTICIPANTS: Five patients undergoing orbital and eyelid biopsy as a diagnostic procedure. METHODS: The authors examined the clinical histories and pathologic findings of five patients with eccrine adenocarcinoma of the eyelid with orbital invasion. MAIN OUTCOME MEASURES: Clinical and histopathologic examinations, including routine histopathology, immunohistochemistry, and electron microscopy studies. RESULTS: The tumors presented as insidious, diffusely infiltrative, firm cutaneous masses in the periocular area that later infiltrated the orbit. Histopathologic examination revealed that the tumors infiltrated the dermis and were composed of cells with a histiocytic to signet ring appearance. Tumor cells exhibited intracellular mucin production. Immunohistochemical stains were positive in tumor cells for low and high molecular weight cytokeratins, carcinoembryonic antigen, and epithelial membrane antigen. Electron microscopic examination showed lumen formation and intracytoplasmic mucin in tumor cells. CONCLUSIONS: The histiocytoid variant of adenocarcinoma of the eccrine sweat gland of the eyelid may present as an insidious tumor and diffusely invade the orbit. These cases may be confused with metastatic adenocarcinoma.

Adenocarcinoma↗

PubMed2000

Sweat gland function as a measure of radiation change.

BACKGROUND: Radiotherapy may result in dryness of the skin even when no other change can be detected. We describe a system for recording the electrical conductance of skin as a measure of sweat gland function. PATIENTS AND METHODS: In 22 normal volunteers close agreement was obtained between measurements obtained from comparable sites on both sides of the chest. Measurements were subsequently made in 38 patients treated by radiotherapy to one side of the chest for tumours of the breast or lung using one of five different fractionation schedules. Simultaneous readings were obtained from both sides of the chest with the non irradiated side acting as a control. RESULTS: A dose response relationship was demonstrated: five patients who received the equivalent total dose of 15 Gy in 2-Gy fractions showed no change in conductance. Sixteen out of 23 who received an equivalent total dose of 42-46 Gy in 2-Gy fractions had a greater than 22% reduction in mean skin conductance compared with that of the control areas despite the skin appearing normal in the large majority. Marked changes in skin conductance were seen after higher total doses. In a prospective study 18 women receiving breast irradiation underwent weekly readings during treatment. A mean reduction of 40% in skin conductance was noted by the end of the second week of treatment prior to any clinical evidence of radiation change. Skin conductance returned to normal in 44% of patients by 6 months. In the remainder, those patients who showed the greatest reduction in skin conductance during treatment demonstrated the least recovery. CONCLUSIONS: Changes in sweat gland function can be detected and quantified in skin which may otherwise appear normal. Differences may so be demonstrated between areas treated using different fractionation schedules and the method may be applied to the detection during radiotherapy of unusually sensitive patient.

Adult↗

PubMed1987

Expression of neural-tissue markers (S-100 protein and Leu-7 antigen) by sweat gland tumors of the skin. An immunohistochemical study.

The expression of two immunohistochemical markers of neural tissue and of cutaneous sweat glands (S-100 protein and Leu-7 antigen) was studied in a group of cutaneous epithelial, mostly glandular, tumors. These antigens were detected only on sweat gland neoplasms showing both types (eccrine and apocrine) of differentiation. These results suggest that antibodies to S-100 protein and to Leu-7 antigen have a limited value in the distinction between eccrine and apocrine tumours, but they may serve as a useful adjunct for the confirmation of the glandular differentiation of cutaneous epithelial tumors.

Antigens, Differentiation, T-Lymphocyte↗

PubMed1979

Lacrimal sweating in a patient with Reader's syndrome.

A patient is described with a right-sided Reader's syndrome and extensive sweating in the frontal area above the right eye. This pathological sweating can be explained by sprouting of lacrimal parasympathetic fibres in the previous denervated sympathetic sudomotor pathways to that area.

Adult↗

PubMed1998

Testing for drugs of abuse in saliva and sweat.

The detection of marijuana, cocaine, opiates, amphetamines, benzodiazepines, barbiturates, PCP, alcohol and nicotine in saliva and sweat is reviewed, with emphasis on forensic applications. The short window of detection and lower levels of drugs present compared to levels found in urine limits the applications of sweat and saliva screening for drug use determination. However, these matrices may be applicable for use in driving while intoxicated and surveying populations for illicit drug use. Although not an illicit drug, the detection of ethanol is reviewed because of its importance in driving under the influence. Only with alcohol may saliva be used to estimate blood levels and the degree of impairment because of the problems with oral contamination and drug concentrations varying depending upon how the saliva is obtained. The detection of nicotine and cotinine (from smoking tobacco) is also covered because of its use in life insurance screening and surveying for passive exposure.

Biological Transport↗