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PubMed2006

Effect of measurement noise and electrode density on the spatial resolution of cortical potential distribution with different resistivity values for the skull.

The purpose of the present theoretical study was to examine the spatial resolution of electroencephalography (EEG) by means of the accuracy of the inverse cortical EEG solution. The study focused on effect of the amount of measurement noise and the number of electrodes on the spatial resolution with different resistivity ratios for the scalp, skull and brain. The results show that if the relative skull resistivity is lower than earlier believed, the spatial resolution of different electrode systems is less sensitive to the measurement noise. Furthermore, there is then also greater advantage to be obtained with high-resolution EEG at realistic noise levels.

Algorithms↗

PubMed2004

Super-resolution reconstruction of compressed video using transform-domain statistics.

Considerable attention has been directed to the problem of producing high-resolution video and still images from multiple low-resolution images. This multiframe reconstruction, also known as super-resolution reconstruction, is beginning to be applied to compressed video. Super-resolution techniques that have been designed for raw (i.e., uncompressed) video may not be effective when applied to compressed video because they do not incorporate the compression process into their models. The compression process introduces quantization error, which is the dominant source of error in some cases. In this paper, we propose a stochastic framework where quantization information as well as other statistical information about additive noise and image prior can be utilized effectively.

Algorithms↗

PubMed2004

Spatially adaptive high-resolution image reconstruction of DCT-based compressed images.

The problem of recovering a high-resolution image from a sequence of low-resolution DCT-based compressed observations is considered in this paper. The introduction of compression complicates the recovery problem. We analyze the DCT quantization noise and propose to model it in the spatial domain as a colored Gaussian process. This allows us to estimate the quantization noise at low bit-rates without explicit knowledge of the original image frame, and we propose a method that simultaneously estimates the quantization noise along with the high-resolution data. We also incorporate a nonstationary image prior model to address blocking and ringing artifacts while still preserving edges. To facilitate the simultaneous estimate, we employ a regularization functional to determine the regularization parameter without any prior knowledge of the reconstruction procedure. The smoothing functional to be minimized is then formulated to have a global minimizer in spite of its nonlinearity by enforcing convergence and convexity requirements. Experiments illustrate the benefit of the proposed method when compared to traditional high-resolution image reconstruction methods. Quantitative and qualitative comparisons are provided.

Algorithms↗

PubMed2004

MAP estimation for hyperspectral image resolution enhancement using an auxiliary sensor.

This paper presents a novel maximum a posteriori estimator for enhancing the spatial resolution of an image using co-registered high spatial-resolution imagery from an auxiliary sensor. Here, we focus on the use of high-resolution panchomatic data to enhance hyperspectral imagery. However, the estimation framework developed allows for any number of spectral bands in the primary and auxiliary image. The proposed technique is suitable for applications where some correlation, either localized or global, exists between the auxiliary image and the image being enhanced. To exploit localized correlations, a spatially varying statistical model, based on vector quantization, is used. Another important aspect of the proposed algorithm is that it allows for the use of an accurate observation model relating the "true" scene with the low-resolutions observations. Experimental results with hyperspectral data derived from the airborne visible-infrared imaging spectrometer are presented to demonstrate the efficacy of the proposed estimator.

Algorithms↗

PubMed2006

Multigrid tomographic inversion with variable resolution data and image spaces.

A multigrid inversion approach that uses variable resolutions of both the data space and the image space is proposed. Since the computational complexity of inverse problems typically increases with a larger number of unknown image pixels and a larger number of measurements, the proposed algorithm further reduces the computation relative to conventional multigrid approaches, which change only the image space resolution at coarse scales. The advantage is particularly important for data-rich applications, where data resolutions may differ for different scales. Applications of the approach to Bayesian reconstruction algorithms in transmission and emission tomography with a generalized Gaussian Markov random field image prior are presented, both with a Poisson noise model and with a quadratic data term. Simulation results indicate that the proposed multigrid approach results in significant improvement in convergence speed compared to the fixed-grid iterative coordinate descent method and a multigrid method with fixed-data resolution.

Algorithms↗

PubMed2005

First results from the high-resolution mouseSPECT annular scintillation camera.

High-resolution single-photon emission computed tomography (SPECT) imaging in small animals tends to use long imaging times and large injected doses due to the poor sensitivity of single pinhole gamma cameras. To increase sensitivity while maintaining spatial resolution, we designed and constructed a multipinhole collimator array to replace the parallel hole collimators of a Ceraspect human SPECT brain scanner. The Ceraspect scanner is composed of an annular NaI(TI) crystal within which the eight pinhole collimators (1-mm-diameter holes) rotate while projecting nonoverlapping images of the object onto the stationary annular crystal. In this manner, only one-eighth of a collimator rotation is required to acquire a full circle orbit tomographic data set. The imaging field of view (FOV) has a diameter of 25.6 mm in the transverse direction, which is sufficient to encompass a mouse in the transverse direction. The axial FOV is 25.6 mm at the center of the FOV and 13.9 mm at the edge of the transverse FOV. Data are currently acquired in step-and-shoot mode; however, the system is capable of list mode acquisition with the collimator continuously rotating. Images are reconstructed using a cone-beam ordered subsets expectation maximization method. The reconstructed spatial resolution of the system is 1.7 mm and the sensitivity at the center of the FOV is 13.8 cps/microCi. A whole-body bone scan of a mouse injected with [Tc-99 m]MDP clearly revealed skeletal structures such as the ribs and vertebral bodies. These preliminary results suggest that this approach is a good tradeoff between resolution and sensitivity and, with further refinement, may permit dynamic imaging in living animals.

Animals↗

PubMed2006

Incorporation of system resolution compensation (RC) in the ordered-subset transmission (OSTR) algorithm for transmission imaging in SPECT.

In order to reconstruct attenuation maps with improved spatial resolution and quantitative accuracy, we developed an approximate method of incorporating system resolution compensation (RC) in the ordered-subset transmission (OSTR) algorithm for transmission reconstruction. Our method approximately models the blur caused by the finite intrinsic detector resolution, the nonideal source collimation and detector collimation. We derived the formulation using the optimization transfer principle as in the derivation of the OSTR algorithm. The formulation includes one forward-blur step and one back-blur step, which do not severely slow down reconstruction. The formulation could be applicable to various transmission geometries, such as point-source, line-source, and sheet-source systems. Through computer simulations of the MCAT phantom and transmission measurements of the air-filled Data Spectrum Deluxe single photo emission computed tomography (SPECT) Phantom on a system which employed a cone-beam geometry and a system which employed a scanning-line-source geometry, we showed that incorporation of RC increased spatial resolution and improved the quantitative accuracy of reconstruction. In simulation studies, attenuation maps reconstructed with RC correction improved the quantitative accuracy of emission reconstruction.

Algorithms↗

PubMed2005

A learning-based method for image super-resolution from zoomed observations.

We propose a technique for super-resolution imaging of a scene from observations at different camera zooms. Given a sequence of images with different zoom factors of a static scene, we obtain a picture of the entire scene at a resolution corresponding to the most zoomed observation. The high-resolution image is modeled through appropriate parameterization, and the parameters are learned from the most zoomed observation. Assuming a homogeneity of the high-resolution field, the learned model is used as a prior while super-resolving the scene. We suggest the use of either a Markov random field (MRF) or an simultaneous autoregressive (SAR) model to parameterize the field based on the computation one can afford. We substantiate the suitability of the proposed method through a large number of experimentations on both simulated and real data.

Algorithms↗

PubMed2001

NMR of hydrogen bonding in cold-shock protein A and an analysis of the influence of crystallographic resolution on comparisons of hydrogen bond lengths.

Hydrogen bonding in cold-shock protein A of Escherichia coli has been investigated using long-range HNCO spectroscopy. Nearly half of the amide protons involved in hydrogen bonds in solution show no measurable protection from exchange in water, cautioning against a direct correspondence between hydrogen bonding and hydrogen exchange protection. The N to O atom distance across a hydrogen bond, R(NO), is related to the size of the (3h)J(NC') trans hydrogen bond coupling constant and the amide proton chemical shift. Both NMR parameters show poorer agreement with the 2.0-A resolution X-ray structure of the cold-shock protein studied by NMR than with a 1.2-A resolution X-ray structure of a homologous cold-shock protein from the thermophile B. caldolyticus. The influence of crystallographic resolution on comparisons of hydrogen bond lengths was further investigated using a database of 33 X-ray structures of ribonuclease A. For highly similar structures, both hydrogen bond R(NO) distance and Calpha coordinate root mean square deviations (RMSD) show systematic increases as the resolution of the X-ray structure used for comparison decreases. As structures diverge, the effects of coordinate errors on R(NO) distance and Calpha coordinate root mean square deviations become progressively smaller. The results of this study are discussed with regard to the influence of data precision on establishing structure similarity relationships between proteins.

Bacterial Proteins↗

PubMedDate not supplied

Effects of conflict resolution training integrated into a kindergarten curriculum.

The effectiveness of a conflict resolution training program was examined in an American midwestern suburban elementary school. Participants were 80 kindergartners randomly assigned to an experimental or control condition in morning or afternoon time blocks. Children in the experimental condition received 9 hr of conflict resolution training integrated into a curriculum unit on friendship taught daily for 4 consecutive weeks. Children in the control condition were taught the identical friendship unit for the same period of time without conflict resolution training. Teachers rotated equally across conditions. Significant differences between trained and untrained children occurred in their knowledge and retention of the conflict resolution procedure, willingness and ability to use the procedure in conflict situations, and conceptual understanding of friendship.

Child Day Care Centers↗

PubMed2006

The effects of tegaserod on oesophageal function and bolus transport in healthy volunteers: studies using concurrent high-resolution manometry and videofluoroscopy.

BACKGROUND: Tegaserod is a partial 5-hydroxytryptamine 4 receptor agonist with prokinetic effects on the gastrointestinal tract, its effects on oesophageal function are unknown. AIM: A randomized, placebo controlled, double-blind trial assessed the effect of tegaserod on the oesophagus in healthy, asymptomatic subjects. METHOD: A 7-day course of tegaserod 6 mg b.d. vs. placebo was prescribed (n = 17/21 completed both phases of study). High-resolution manometry and pH measurements were performed before and after a test meal. Bolus transport of liquids and solids was studied by high-resolution manometry and videofluoroscopy. RESULTS: Tegaserod had no effect on lower oesophageal sphincter pressure compared with placebo, peristaltic velocity increased (P < 0.001) and distal contractile pressure decreased slightly (P < 0.05). Transient lower oesophageal sphincter relaxations and reflux were infrequent regardless of treatment. During the studies of bolus transport, high-resolution manometry revealed that tegaserod promoted mid-oesophageal contractility (P < 0.02) and shortened the 'proximal transition zone' (P < 0.05), the level where bolus escape occurred most frequently. These effects had no effect on liquid bolus transport; however a non-significant trend to improved solid bolus transport was observed (66% vs. 31%;P = 0.07). CONCLUSION: Tegaserod did not alter lower oesophageal sphincter pressure, but had significant effects on peristaltic function. High-resolution manometry promoted mid-oesophageal contractility during bolus transport. This effect was associated with a non-significant trend to improved solid bolus transit.

Adult↗

PubMed1990

High-resolution electron microscopy of human enamel crystals.

The structure of enamel crystals obtained from four human premolars has been studied by high-resolution electron microscopy (HREM) in the [0001], [2110], [1540], [0110] and [1213] crystallographic directions at various microscope defocus and crystal thickness values. The resolution obtained has not previously been reported for human enamel crystals. In all cases, it was possible to match the experimental images to images calculated using the atomic positions of mineral hydroxyapatite. However, a deviation from hexagonal symmetry characterized by marked (1010) planes of intensity different from the one of the (3030) and (1010)-type planes was observed. In this work, we present an improvement of Scherzer resolution of 0.25-0.20 nm over previous work on biological enamel crystals. This improvement of resolution has permitted the incorporation of crystallographic reflections of higher spatial frequencies into the imaging process of the microscope and has led to a more precise structure determination of the crystals studied.

Adult↗

PubMed1991

Contrast analysis of cryo-images of n-paraffin recorded at 400 kV out to 2.1 A resolution.

n-Paraffin was used as a test specimen for evaluating the relative merits of 400-kV versus 100-kV electron microscopy in recording data for electron crystallographic analysis of beam-sensitive materials. The parameter used for comparison, the relative contrast R, is the ratio of amplitudes from the computed Fourier transform of images and amplitudes from an electron diffraction pattern from the same crystal. R will thus be a measure of the contrast from an experimental image relative to that of a perfect image. Electron diffraction patterns and bright-field images were recorded at 400 kV at a specimen temperature of -167 degrees C. Using the flood-beam imaging technique the best R-value is 0.08 for all reflections in the resolution zone from 4 to 3 A. This value is equivalent to that found at 100 kV. In the resolution zone from 3 to 2A we have found R = 0.02. Using the spot-scan imaging technique, on the other hand, R was measured to be 0.42 for the reflections between 4- and 3-A resolution. This amount of relative contrast is 1.7 times that observed at 100 kV. Reflections at 3-2 A displayed an R-value of 0.05. Besides obtaining higher R-values when applying the spot-scan imaging technique at 400 kV, we observe a higher yield of images with isotropic diffraction and/or higher resolution reflections. Various contrast-attenuating factors, including the modulation transfer function of the photographic film and the cryo-holder, envelope functions for spatial and temporal coherence and lens and high-tension instabilities, the contrast transfer function and lastly the radiation damage effects, have been considered in interpreting the observed image contrast. Overall, use of 400 kV in combination with spot-scan does offer important improvements in contrast levels, which can be very useful in determining the three-dimensional structure from protein crystals.

Crystallography↗

PubMed1995

High resolution HLA-DRB1 SSP typing for cadaveric donor transplantation.

An HLA-DRB1 typing procedure by means of sequence-specific primer (SSP) amplification was developed for 65 different DRB1 subtypes. Subtyping is achieved by the performance of two subsequent PCR assays (PCR-1 assay and PCR-2 assay) using a limited number of reactions. The PCR-1 assay determined low-resolution HLA-DRB1 typing, i.e. the serologically defined specificities DR1, 2, 3, 4, 11, 12, 6, 7, 8, 9 and 10. The second exon of the DRB1 gene is amplified also in this PCR-1 assay. High-resolution subtyping for positively identified alleles was performed in the PCR-2 assay with the exon-2 product from PCR-1 assay as DNA template. PCR reactions were carried out using unpurified primers in reaction volumes of 20 microliters and 100 ng of chromosomal DNA. After 3 hours, the results of the PCR-1 assay were analyzed and subsequently subtyping results in the PCR-2 assay were obtained in another 1.5 hours. A total of 249 DNA samples was typed by this method. No false positive nor false negative results were obtained in DRB1 typing of 32 homozygous cell lines, 56 serologically well-defined panel cells and 125 unrelated individuals. Segregation of the amplification patterns was investigated in 36 members of 7 two-generation families. DRB1 subtyping revealed codominant Mendelian segregation for all subtypes investigated. In conclusion, LR-HR-PCR-SSP typing is a fast and reliable typing technique for routine DNA typing purposes which gives complete DRB1 subtyping within 4.5 h. Besides low-resolution DRB typing, also high-resolution DRB subtyping for prospective HLA-DR matching in cadaveric renal transplantation is possible by this method.

Alleles↗

PubMed1991

The solution structures of Escherichia coli trp repressor and trp aporepressor at an intermediate resolution.

We have determined the solution structures and examined the dynamics of the Escherichia coli trp repressor (a 25-kDa dimer), with and without the co-repressor L-tryptophan, from NMR data. This is the largest protein structure thus far determined by NMR. To obtain a set of data sufficient for a structure determination it was essential to resort to isotopic spectral editing. Line broadening observed in this molecular mass range precludes for the most part the measurement of coupling constants and stereospecific assignments, with the inevitable result that the attainable resolution of the final structure will be somewhat lower than the resolution reported for smaller proteins and peptides. Nevertheless the general topology of the protein can be deduced from the subsets of NOEs defining the secondary and tertiary structure, providing a basis for further refinement using the full set of NOEs and energy minimization. We report here (a) an intermediate resolution structure that can be deduced from NMR data, covalent, angular and van-der-Waals constraints only, without resort to detailed energy calculations, and (b) the limits of uncertainty within which this structure is valid. An examination of these structures combined with backbone amide exchange data shows that even at this resolution three important conclusions can be drawn: (a) the protein structure changes upon binding tryptophan; (b) the putative DNA binding region is much more flexible than the core of the molecule, with backbone amide proton exchange rates 1000 times faster than in the core; (c) the binding of tryptophan stabilizes the repressor molecule, which is reflected in both the appearance of additional NOEs, and in the slowing of backbone proton exchange rates by factors of 3-10. Sequence-specific 1H-NMR assignments and the secondary structure of the holopressor (L-tryptophan-bound form) have been reported previously [C. H. Arrowsmith, R. Pachter, R. B. Altman, S. B. Iyer & O. Jardetzky (1990) Biochemistry 29, 6332-6341]. Those for the trp aporepressor (L-tryptophan-free form), made using the same methods and conditions as described in the cited paper, are reported here. The secondary structure of the aporepressor was calculated from sequential and medium-range NOEs and is the same as reported for the holorepressor except that helix E is shorter. The tertiary solution structures for both forms of the repressor were calculated from long-range NOE data.(ABSTRACT TRUNCATED AT 400 WORDS)

Amino Acid Sequence↗

PubMed2006

Macrophages of the bovine heifer mammary gland: morphological features during initiation and resolution of the inflammatory response.

This work characterizes macrophage morphological features during initiation and resolution of an inflammatory response by the bovine mammary gland. The study has been carried out in 20 mammary glands of five virgin heifers by using light microscopy of natural and stained cells and by using transmission electron microscopy (TEM). The inflammatory reaction was induced by an intramammary administration of phosphate-buffered saline (PBS). It has been found that both the initial as well as the resolution phases of the inflammatory reaction are characteristic of the presence of various morphologically different macrophage forms. During the initial phase of the inflammatory response, the major proportion of the macrophage population consisted of monocyte-like macrophages, which represented newly migrated cells. These macrophages were 12-15 mum in size, with spherical or ovoidal shapes, and contained homogenous, fine-granular cytoplasm rich in Golgi complexes, numerous mitochondria, and no lysosomes. The nuclei of the macrophages were kidney-shaped, and surrounded by dark chromatin along the peripheries. Macrophages with phagocytosed apoptotic neutrophils in the cytoplasm were detected already during the initial phase. These macrophages reached the highest proportion 48-72 h after the influx induction and participated in the resolution of the inflammatory reaction. Other cells, also detected during the resolution of the inflammatory reaction, were vacuolized macrophages that formed the largest cells in the lavages of the mammary glands and that were structurally characteristic for the presence of vacuoles in the cytoplasm. In TEM the macrophage vacuoles formed both phagolysosomes with residues of pre-digested material of phagocytosed apoptotic neutrophils and vacuoles that were less electon-dense. Morphologically different forms of macrophages reflected their real-time functions in the inflammation process.

Animals↗

PubMed2005

NKT cells are not critical for HSV-1 disease resolution.

NKT cells are a minor subset of T cells that have important roles in controlling immune responses in disease states including cancer, autoimmunity and pathogenic infections. In contrast to conventional T cells, NKT cells express an invariant TCR and respond to glycolipids presented by CD1d. In this study, we sought to investigate the role of NKT cells in regulating the response to infection with HSV-1, and the mechanism involved, in well-established mouse models. Previous studies of HSV-1 disease in mice have shown clear roles for CD4+ and CD8+ T cells. The role of NKT cells in the resolution of HSV-1 (KOS strain) infection was investigated through flank zosteriform or footpad infection in wild-type versus CD1d-deficient mice, by measurement of viral plaque-forming units at different sites after infection, lesion severity and HSV-1-specific T-cell responses. In contrast to a previous study using a more virulent strain of HSV-1 (SC16 strain), no differences were observed in disease magnitude or resolution, and furthermore, the T-cell response to HSV-1 (KOS strain) was unaltered in the absence of NKT cells. In conclusion, this study shows that NKT cells do not play a general role in controlling the resolution or severity of HSV-1 infection. Instead, the resolution or severity of the infection may depend on the HSV-1 strain under investigation.

Animals↗

PubMedDate not supplied

In vitro measurement of rabbit corneal epithelial thickness using ultrahigh resolution optical coherence tomography.

The objective of this study was to reproducibly measure corneal epithelial thickness centrally and at the limbus in the rabbit cornea using ultrahigh resolution optical coherence tomography (OCT). Twelve freshly enucleated New Zealand white rabbit eyes were kept in a moist chamber at 4 degrees C. An ultrahigh resolution OCT system with a spatial resolution of 1.3 microm was used to image the cornea and its component layers. The central and peripheral (limbal) regions of all the samples were scanned within 6 h of harvest in order to minimize the post-mortem degradation of the corneal epithelium. The thickness of the corneal epithelium was determined by measuring the pixel equivalents of the obtained image. Unpaired Student's t-test was used to evaluate differences. The epithelial thickness centrally was found to be 45.8 +/- 2.2 microm, and 37.6 +/- 1.4 microm at the limbus (P < 0.001). Rabbit corneal epithelium is thicker centrally than at the limbus when measured by ultrahigh resolution OCT. This technique will aid in delineating the pathophysiology of diseases of the anterior cornea.

Animals↗