Chemical decontamination of gutta-percha cones with polyvinylpyrrolidone-iodine.
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The effect of the treatment with various concentrations (2%, 5%, 7.5% and 10% w/v) of lactic acid/sodium lactate buffer (pH 3.0) combined with modified atmosphere packaging (MAP) (90% CO2/10% O2) on the shelf life and organoleptic quality of fresh chicken legs stored at 6 degrees C was investigated. The CO2 concentration of all samples packed in modified atmosphere (MA) decreased during the first 3 days of storage, followed by a gradual increase after the third day, while O2 showed a corresponding decrease. The buffering capacity of the buffer systems seem to be sufficient to maintain a low pH of the skin during storage. Legs treated with 2, 5, 7.5, and 10% (w/v) lactic acid/sodium lactate buffer (pH 3.0) combined with MAP have a shelf life at 6 degrees C of 14, 15, 16 and 17 days, respectively. The shelf life when the product was not treated with lactic acid was 1, 2.3 and 4 days shorter, respectively.
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The Wolf 35100 and DSD-91 endoscope disinfection machines were compared. Using a total viable count method, the degree of contamination of an Olympus GIFXQ20 endoscope was assessed following inoculation with a suspension of Pseudomonas aeruginosa and again following disinfection cycles in each machine, using 2% glutaraldehyde as the disinfectant. Both machines performed satisfactorily, with an average reduction of between 7 and 8 log10 cfu ml-1 P. aeruginosa following disinfection. The dilution effect on the glutaraldehyde of repeated disinfection cycles was also assessed for each machine. After 40 cycles in the DSD-91 and 25 cycles in the Wolf 35100 machines, the dilution of glutaraldehyde may give rise to concern, especially if adequate pre-cleaning of the endoscope is not carried out. The DSD-91 machine has certain advantages over the Wolf 35100 in terms of endoscope capacity, running costs and ease of maintenance. Both machines give similar results for endoscope disinfection.
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