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PubMed2001

Ran binding protein RanBP1 in zebrafish embryonic development.

Ran, which functions in nucleocytoplasmic transport and mitosis, binds to and is regulated in part by RanBP1. We have identified a zebrafish RanBP1 cDNA and report that it encodes for a polypeptide of 233 amino acids with considerable similarity to human and Xenopus RanBP1, despite the fact that it is 10% longer due to an extension at its carboxy terminus. RanBP1 mRNA is present as a maternal transcript and is expressed ubiquitously throughout the developing embryo. At the protein level, RanBP1 is present at all embryonic stages. Surprisingly, the ectopic overexpression of the protein had no obvious effect on embryogenesis. Attempts were also made to down-regulate RanBP1 activity by RNA interference. Injecting double-stranded RNA augmented both the mortality rate and the frequency of induced defects. Specific defects accompanied by changes in RanBP1 expression were not seen, leading us to propose that RNAi is not a reliable method for deregulating the activity of constitutively expressed genes, like RanBP1, in zebrafish. Mol. Reprod. Dev. 59:235-248, 2001.

Amino Acid Sequence↗
PubMed2004

Post-embryonic development of chicks of the Japanese quail under conditions of the limited volume.

According to our results, chicks of the Japanese quail can survive within the first 10 days of a life under conditions of the limited volume when their mobility is completely limited. However, their development was impaired in comparison with chicks housed in the common cages. Besides, the delay in development of chick's plumage has been observed. The analysis of body mass in test groups has shown that practically all chicks were smaller than chicks from control group. It is the result of feed consumption decrease (about 30% from control group) caused by the restriction of mobility of chicks. But after their transferring to the common cages at the end of the experiment, body mass in test group rapidly reached the level of body mass in control group. Research has shown that percent of alive of quail chicks maintained in the chamber of animals of complex "Incubator-2", was higher than in first two experiments. Therefore, works on improvement of devices for the maintenance of quail chicks continue, and also new adaptations develop for the maintenance of chicks in their early post-embryonic period (till 10 days) with reference to conditions of weightlessness.

Animals↗
PubMed1977

Gliogenesis during embryonic development in the rat.

With the aid of thymidine-H3 autoradiography gliogenesis in the rat brain was seen to start during embryonic stages, which might continue into the postnatal stages of development. Gliogenesis followed a caudo-rostral gradient closely following neurogenesis. Ependymogenesis was found to occur in parallel with gliogenesis.

Animals↗
PubMed2006

YB-1 is important for an early stage embryonic development: neural tube formation and cell proliferation.

The eukaryotic Y-box-binding protein-1 (YB-1) is involved in the transcriptional and translational control of many biological processes, including cell proliferation. In clinical studies, the cellular level of YB-1 closely correlates with tumor growth and prognosis. To understand the role of YB-1 in vivo, especially in the developmental process, we generated YB-1 knock-out mice, which are embryonic lethal and exhibit exencephaly associated with abnormal patterns of cell proliferation within the neuroepithelium. beta-Actin expression and F-actin formation were reduced in the YB-1 null embryo and YB-1(-/-) mouse embryonic fibroblasts, suggesting that the neural tube defect is caused by abnormal cell morphology and actin assembly within the neuroepithelium. Fibroblasts derived from YB-1(-/-) embryos demonstrated reduced growth and cell density. A colony formation assay showed that YB-1(-/-) mouse embryonic fibroblasts failed to undergo morphological transformation and remained contact-inhibited in culture. These results demonstrate that YB-1 is involved in early mouse development, including neural tube closure and cell proliferation.

Animals↗
PubMed2005

Role of Fabp7, a downstream gene of Pax6, in the maintenance of neuroepithelial cells during early embryonic development of the rat cortex.

Pax6 is a transcription factor with key functional roles in the developing brain. Pax6 promotes neuronal differentiation via transcriptional regulation of the Neurogenin2 (Ngn2) gene, although Pax6 expression appears in proliferating neuroepithelial cells before the onset of neurogenesis. Here, we identified Fabp7 (BLBP/B-FABP), a member of the fatty acid-binding protein (FABP) family, as a downregulated gene in the embryonic brain of Pax6 mutant rat (rSey2/rSey2) by microarray analysis. Marked reduction of Fabp7 expression was confirmed by quantitative PCR. Spatiotemporal expression patterns of Fabp7 in the wild-type rat embryos from embryonic day 10.5 (E10.5) to E14.5 were similar to those of Pax6, and expression of Fabp7 was undetectable in the rSey2/rSey2 cortex. The expression pattern of Fabp7 in the wild-type mouse embryo at E10.5 (corresponding to E12.5 rat) was different from that in the rat embryo, and no change of expression was observed in the Sey/Sey mouse embryo. Overexpression of exogenous Pax6 mainly induced ectopic expression of Fabp7, rather than of Ngn2, in the early cortical primordium. Interestingly, knocking-down FABP7 function by electroporation of Fabp7 small interfering RNA severely curtailed cell proliferation but promoted neuronal differentiation. We conclude that Fabp7 is a downstream gene of Pax6 transcription factor in the developing rat cortex and essential for maintenance of neuroepithelial cells during early cortical development.

Animals↗
PubMed1985

Mouse teratocarcinoma and embryonic development. Two-dimensional protein patterns in muscle differentiation.

Muscle cell differentiation was analysed by two-dimensional gel electrophoresis of newly synthesized proteins compared in two parallel systems: in mouse teratocarcinoma-derived tumors that became restricted in their developmental potential to the formation of muscle-like cells and in developing limbs of the early mouse fetus. Muscle cell differentiation in teratocarcinomas was found to be reflected by both a marked decrease in the rate of synthesis of about 12% (119 proteins) of the resolved polypeptides and a pronounced increase in the synthesis of another large set of proteins (83 proteins). The majority of the newly acquired proteins (46 proteins) were also detected in fetal brain and muscle tissue. These proteins are considered to be those which accompany differentiation in general, regardless of cell type, as e.g. ubiquitously occurring structural proteins. Their expression in the muscle cells of the tumors may reflect the normal aspect of this particular differentiation pathway. Five polypeptides were found to appear specifically in both myogenic tumors and developing limbs, but not during brain formation and were tentatively termed muscle-specific proteins (MSP). The identification of differentiation-related proteins in muscle development will allow us to analyse differentiation in early development in molecular terms.

Animals↗