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At least 1,387 records · Page 77Linked to original sources
PubMed2004

Decontamination of broilers with hydrogen peroxide stabilised with glycerol during processing.

Experiments were conducted to assess the effects of different concentrations of hydrogen peroxide stabilised by glycerol solution in potable water on the bacteriological and organoleptic quality of freshly slaughtered broiler carcasses. Skin-pH and colour were measured 3.5 and 24 h after treatment and compared to untreated carcasses. Bacterial colonisation was determined 3.5 and 24 h and, 7 days after treatment, carcasses being stored at 1 degrees C. None of the concentrations used affected the appearance and "bloom" of the carcasses as could be measured by colorimeter and changing of the acidity. Mean microbial counts were significantly reduced (P<0.01) when treated and control broilers were compared. Average reductions of 0.3 up to1.4 log N for the mesophilic aerobic counts were achieved and from 0.4 up to 1.2 log N for Enterobacteriaceae. A 3% w/w solution made from a commercially available stock solution (Glyroxyl), which consists of 44% hydrogen peroxide, 44% demineralised water and 12% glycerol proved to lower colonisation more effectively than a 2% solution.

Animals↗
PubMed2002

External contamination of bovine hair with beta2-agonist compounds: evaluation of decontamination strategies.

Hair analysis has shown great potential in the control of illegal use of veterinary drugs such as beta2-agonists. However, it has been shown that hair can be externally contaminated with drugs which can lead to false positive results. Exposure of bovine hair to aqueous solutions of beta2-agonist compounds results in incorporation of these drugs into the hair. Standard hair washing procedures found in the literature: detergent (Tween-20), phosphate buffer or organic solvents (dichloromethane or methanol) cannot eliminate this external contamination. Beta2-agonists can be extracted from hair very efficiently with 0.1 M HCl, the extraction kinetics of externally and endogenously accumulated clenbuterol at room temperature are different which makes it feasible to discriminate between them. Treatment of hair samples with a 0.1 M HCI solution for 2 h at room temperature results in a ratio of clenbuterol content in the wash solution to clenbuterol content in the washed hair equal to or less than 0.25 for samples from treated cattle; whereas this ratio is equal to or higher than 0.70 for externally contaminated samples. The design of the study was intended to resemble the plausible scenario of hair being sampled a short time after external contamination. A similar study to detect external contamination for hair sampled a long time after exposure is in progress.

Adrenergic beta-2 Receptor Agonists↗
PubMed1997

Distribution of bacteria on hands and the effectiveness of brief and thorough decontamination procedures using non-medicated soap.

Our perception of the role of hand washing in the clinical situation is based on experimental studies in which test-bacteria are usually inoculated onto the skin surface and removed using hand washing preparations containing antiseptics. In this study, we have investigated the distribution of bacteria on the hands of volunteers and the effectiveness of long (3 minute) and brief (10 second) washes in removing both naturally-occurring and artificially-inoculated bacteria (Micrococcus sp.), using only soap and water. There was a tenfold reduction in median counts of artificially inoculated bacteria following both long and brief washes. However, less than 50% of naturally-occurring bacteria were removed and, for hands previously disinfected by immersion in 70% ethanol, the washing procedure increased bacterial counts. In both unwashed hands, and hands washed following a strict protocol, the mean variation in counts of naturally-occurring bacteria at different sites (wrists, dorsal surface, palmar surface, fingertips and interdigital spaces) was only two-fold. The efficiency of recovery of naturally-occurring organisms was estimated by repeated swabbing, to be more than 60%. The data question the value of typical hand wash procedures recommended by many authorities for use in clinical situations and of the perfunctory hand washes frequently adopted by nursing staff in busy wards. Experimental evidence is required to justify procedures and to identify the precise circumstances in which they are of value.

Disinfectants↗
PubMed2000

Decontamination of a Mycoplasma-infected Chlamydia pneumoniae strain by pulmonary passage in SCID mice.

We describe a procedure to eliminate contaminating Mycoplasma from Chlamydia pneumoniae (C. pneumoniae) cultures by pulmonary passage in severe combined immunodeficiency mice (SCID). Four weeks after experimental infection only C. pneumoniae could be cultured from the lungs of the infected animals while Mycoplasma could not be detected any longer, as shown by PCR, culture and transmission electron microscopy (TEM).

Animals↗
PubMed1980

The dose at which neomycin and polymyxin B can be applied for selective decontamination of the digestive tract in mice.

Oral treatment of mice with various doses of neomycin or polymyxin B was performed in order to determine which dose caused substantial suppression of aerobic gram-negative rods. In addition the effect of the various doses on Streptococcus faecalis and on other factors of the colonization resistance (CR) of the digestive tract were studied. It was found that polymyxin B was effective in suppressing sensitive gram-negative bacteria following daily doses of 3.2 mg/mouse, and that even extremely high daily doses of 9.7 mg/mouse did not affect the CR. Neomycin was effective in suppressing Enterobacteriaceae species following oral daily doses of 5.4 mg/mouse. With this dose, however, the CR was somewhat decreased which was also evidenced by the increased concentration of beta-aspartylglycine in the faeces and the increased size (weight) of the caecum in these animals. Suppression of Str. faecalis was seen from doses of 24 mg/mouse on.

Animals↗
PubMed1974

Clearence of antibiotics from the intestines after termination of antibiotic decontamination.

The clearance of neomycin and kanamycin from the intestines after stopping oral supply has been determined in mice. Both antibiotics, although given in different doses, were excreted in essentially the same way; the clearance being a little faster than logarithmically in both cases. The importance of this observation with regard to isolation and the moment of reconventionalization is discussed.

Animals↗