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PubMed1987

Transneuronal transport of wheat germ agglutinin-conjugated horseradish peroxidase into trigeminal interneurones of the rat.

Intramuscular injections of either horseradish peroxidase (HRP) or wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) were made into the masseter muscle of rats. Both tracers labeled primary sensory neurones in the V mesencephalic nucleus, motoneurones in the V motor nucleus, and some motoneurones in the facial motor nucleus. WGA-HRP labeled additional neurones in the V main sensory nucleus and the rostral pole of the V nucleus oralis. These were classed as interneurones because they lay in areas outside those known to contain either first-order afferent or motoneurone somata. We argue that these were labeled by retrograde transport of tracer because they lay close to the V motor nucleus, and from some of them processes could be followed into the region of the V motor nucleus.

Animals↗

PubMed1989

Distribution and central projections of primary afferent neurons that innervate the masseter muscle and mandibular periodontium: a double-label study.

A double-label strategy was used to determine the distribution and central projections of primary afferent neurons that innervate the periodontium and muscles of mastication in cats. Central injections of either Fast Blue (FB) or a mixture of wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) and HRP were made into one of three cytoarchitectonically distinct regions of the spinal trigeminal nucleus. These regions included the subnucleus oralis (Vo), the subnucleus interpolaris (Vi), and the medullary dorsal horn (MDH). In each case, injections were also made into the periodontium of the ipsilateral mandibular teeth or into the ipsilateral masseter muscle. FB injections preceded the peroxidase injections by at least 48 hours and total survival time ranged from 72 to 96 hours. Animals were perfused with phosphate-buffered paraformaldehyde (4%; pH 7.2). Serial frozen sections were made through the brainstem and trigeminal ganglion. Tetramethylbenzidine was used as a chromagen to demonstrate HRP and sections were viewed with brightfield and epifluorescent illumination. Cells containing peripherally injected tracer were observed in the lateral portion of the ganglion and in the mesencephalic nucleus (Vmes). Double-labeled ganglion cells were observed in most cats that received periodontal injections in combination with central injections in the dorsal part of spinal trigeminal nucleus regardless of the rostrocaudal level of the central injection. In the animals that received intramuscular injections, double-labeled ganglion cells were observed only in the animals that received central injections caudal to the Vo. Double-labeled Vmes perikarya were observed in cats that received either intramuscular or periodontal injections in combination with central injections into the MDH and Vo but not in animals that received injections into the Vi. These results demonstrate that ganglion cell periodontal afferents project to the three major rostrocaudal subdivisions of the spinal trigeminal nucleus while ganglion cell muscle afferents have more limited central projections to caudal regions of the nucleus. Masseter and periodontal Vmes afferents also project ot the spinal trigeminal nucleus--specifically, to the Vo and MDH. These findings are consistent with physiological observations regarding the role of periodontal and masseteric afferents in oral and facial reflexes and somesthetic mechanisms.

Afferent Pathways↗

PubMed1989

Neural organization of the masseter muscle in the pig.

The neural organization of the pig masseter, an architecturally and functionally compartmentalized muscle, was investigated by using dissection, glycogen depletion, evoked electromyography, and counts of axon numbers at various levels along the masseteric nerve. The masseteric nerve enters the muscle as two rostral branches, which also supply the zygomatico-mandibularis, and a more caudal main branch, which soon divides into four terminal nerves with variable distributions. Stimulation of filaments containing roughly 50 extrafusal motor axons resulted in glycogen depletion of 5-20% of the muscle fibers in very small subvolumes of the masseter; the affected subvolumes were delimited by perimysium. Electromyography after stimulation of various branches of the nerve confirmed the distributions deduced from anatomy and further indicated that axons do not branch between the rostral and main nerve branches but may occasionally do so among the more distal terminal branches of the main branch. The proximal trunk of the masseteric nerve contains about 3,500 myelinated fibers with a bimodal size distribution. Approximately 1,000 of the larger fibers were estimated to be extrafusal motor axons. Along the proximal trunk of the nerve, fibers were constantly rearranged; coupled with the observation that the locations of motor unit territories were usually not related to the position of the stimulated axons within the nerve, this suggests that the nerve trunk is not strictly ordered somatotopically.

Action Potentials↗

PubMed1991

Morphological characteristics and terminating patterns of masseteric neurons of the mesencephalic trigeminal nucleus in the rat: an intracellular horseradish peroxidase labeling study.

In order to study the morphological characteristics and terminating patterns of the neurons of the trigeminal mesencephalic nucleus (Vme), 55 masseteric neurons in Vme in the rat were stained by intracellular injection of horseradish peroxidase (HRP). Labeled cells were distributed throughout the nucleus. These neurons were divided into three types: uni- or pseudounipolar (type A, n = 43), bipolar (type B, n = 5), and multipolar cells (type C, n = 7). Each type was further divided into two subtypes according to the largest diameter of the perikarya (type a greater than or equal to 30 microns, type b less than 30 microns). The central processes of type Aa neurons projected to the following three groups of target nuclei: 1) nuclei functioning as interneurons, including supratrigeminal nucleus (Vsup), intertrigeminal nucleus (Vint), juxta-trigeminal region (Vjux), and parvicellular nucleus of the pontomedullary reticular formation (PcRF); 2) motor nuclei, including the trigeminal motor nucleus (Vmo), accessory facial nucleus (NVIIacs), accessory abducens nucleus (NVIacs), and a small number of labeled axons in the oculomotor nucleus and trochlear nucleus; 3) sensory nuclei, including the dorsomedial part of the principal trigeminal sensory nucleus (Vpdm) and the dorsomedial part of subnucleus oralis of the trigeminal spinal nucleus (Vodm). Labeled processes were dense in the Vsup, Vmo, and Vpdm. The proprioceptive pathway of the fifth nerve is discussed. Direct projections from type Aa neurons of Vme to the Vpdm and dorsolateral part of the Vsup contribute to conduction of the proprioceptive information from spindles of masticatory muscle to the contralateral thalamus in the rat. Different axon morphology, distribution, terminal branch density, and terminating patterns of type Aa neurons were noted in different functional groups of the projecting nuclei, especially in the Vsup, Vmo, and Vpdm. The highest terminal branching density, the most extensive distribution, and two different types of branching patterns (claw-like and comb-like) were observed in Vsup. Selective distribution and single-beaded or "Y"-shaped terminal branches were observed in Vmo. In the Vppdm the axonal branches were sparser than in the Vsup or Vmo, and had an arrangement like the branches of a weeping willow tree. These characteristics of anatomical organization might be related to the function of each projecting nucleus.

Animals↗

PubMed1993

Development of the mesencephalic nucleus of the trigeminal nerve in chick embryos: target innervation, neurotrophin receptors, and cell death.

The goal of this study was to determine whether processes of neurons in the mesencephalic nucleus of the trigeminal nerve (Mes V) of chick embryos arrive in their peripheral target prior to the period of developmental cell death, and to determine whether neurons with early target contact survive to a greater extent than neurons with processes that reach their peripheral target later. The arrival of Mes V nerve fibers in the masticatory muscles was determined by injecting the fluorescent tracer DiI, and the position of labeled and unlabeled neurons was mapped in subdivisions of the Mes V nucleus. Developmental changes in the numerical configuration of Mes V subdivisions were studied in DiI-labeled as well as Nissl-stained material. The expression of low-affinity (p75) neurotrophin receptors was investigated throughout development of the Mes V nucleus with in situ hybridization to assess whether and how levels of expression of this trophic receptor may relate to target innervation and cell death. The extent of cell death was evaluated by counting pyknotic nuclei. Processes of Mes V neurons invade their peripheral target between 5 and 7 days of incubation (E5-7). At E7-12, between 800 and 1,400 labeled Mes V neurons were distributed throughout the two main subdivisions of the Mes V nucleus, the tectal commissure and the optic tectum. Only few Mes V neurons were labeled in the posterior commissure or outside the brain. Cell counts in Nissl-stained material from E7-13 revealed that the numbers of Mes V neurons in the optic tectum decreased to about 40-60%, and in the tectal commissure to 20-25%, whereas Mes V neurons in the posterior commissure disappeared almost entirely. Few Mes V neurons remained in the leptomeninges at E8-10, but a considerable number was found outside the midbrain at E11, indicating ongoing migration of some Mes V neurons. Neurotrophin receptors were differentially expressed in the Mes V nucleus: Before and after the period of cell death, 90-100% of Mes V neurons expressed neurotrophin receptors, whereas during, and immediately preceding the period of developmental cell death (E9-E13), merely 70% of Mes V neurons expressed this receptor. These findings are consistent with the hypothesis that early target contact may provide an advantage for the survival of Mes V neurons and that competition for trophic factors may occur in the peripheral target of this nucleus prior to the period of cell death.

Animals↗

PubMed1992

Jaw-muscle activity in ferrets, Mustela putorius furo.

Electromyographical (EMG) activity was recorded bilaterally from the masseter and temporalis muscles of alert ferrets (Mustela putorius furo) during mastication and crushing. Electromyographic activity was also recorded during biting while a bite-force transducer placed between the carnassial teeth registered forces ranging from 1.5 to 48.8 N. Linear regression analysis demonstrates that temporalis and masseter EMG activity are linearly related to bite force. Electromyographic activity from the balancing-side muscles is nearly equal to EMG activity of the working-side muscles during bone crushing with the carnassial teeth. It is hypothesized that a high percentage of balancing-side muscle activity in ferrets can be recruited during carnassial biting because the postglenoid process prevents ventral displacement of the working-side mandibular condyle.

Animals↗

PubMedDate not supplied

Anterior disc displacement without reduction in the temporomandibular joint: MRI and associated clinical findings.

To determine the value of MRI in temporomandibular joint (TMJ) disorders, the data of MRI-proven anterior disc dislocation without reduction (ADWOR) were correlated with clinical history and clinical data. MRI demonstrated degenerative bony changes and a reduced sagittal diameter of the condyle, a variable degree of disc deformation, and a thinned bilaminar zone in each of the joints with ADWOR, which clearly differed from patients with anterior disc dislocation with reduction (ADWR) (P = .01) and normal disc position (NDP) (P < .001). Of 59 patients and 83 TMJs that had been investigated in a 2-year period, as shown by MRI, 22 patients (27 TMJs) had ADWOR (32%), 16 joints had NDP (19%), and 40 patients had ADWR (49%). In patients with ADWOR, the clinical history revealed pain in either of the joints and/or cervical or masticatory muscles in 25 (93%) joints. Clinical investigation revealed various abnormalities in 22 joints; five of those presented without any pathologic clinical finding. Mouth opening was unlimited in nine patients (47%), palpation of the muscles of mastication was painless in 13 patients (52%), and joint noises during mouth opening or closing were noted in 14 patients (56%). According to clinical histories, four patients were suspected to have become symptomatic only after dental treatment. ADWOR is difficult to diagnose with clinical methods alone. The indication for MRI evaluation of the TMJ should be extended for asymptomatic patients with a history of limitation in mouth opening and pathologic x-ray morphology of the condyle. Because symptoms may arise after dental treatment in these patients, aggravation of internal derangement may be avoided by careful handling.

Adult↗

PubMed2006

Alteration of central motor excitability in a patient with hemimasticatory spasm after treatment with botulinum toxin injections.

Hemimasticatory spasm (HMS) is a condition characterized by paroxysmal involuntary contraction of masticatory muscles. We performed an electrophysiological investigation of a single patient with HMS to identify any pathophysiological changes associated with the condition. We identified a delayed M wave and jaw jerk on the affected side and an absent masseteric silent period during spasm. Botulinum toxin injections successfully treated the clinical symptoms and resulted in a significant reduction in the excitability of the blink reflex recovery cycle. These data suggest that HMS may be due to ectopic activity in the motor portion of the trigeminal nerve that is capable of inducing changes in the excitability of central reflex pathways. These changes can be altered by successful treatment with botulinum toxin.

Blinking↗

PubMed2004

Canine inflammatory myopathy: analysis of cellular infiltrates.

Inflammatory myopathies (IMs) occur relatively frequently in dogs, and, with the exception of masticatory muscle myositis (MMM), have not been characterized. This study analyzed the distribution and types of cellular infiltrates in 21 cases of generalized IM, 3 cases of focal IM (MMM), and 1 case with features of both generalized and focal IM, using a panel of monoclonal antibodies to cell surface markers. In generalized IM, mononuclear cells showed an endomysial and perimysial distribution with invasion of non-necrotic fibers similar to human IM. T lymphocytes with T-cell receptor (TCR)alphabeta predominated. Distinct differences were seen in MMM including prominent B-cell infiltration, dendritic cells and macrophages in greater numbers than T cells, and numerous T cells with TCRgammadelta. Thus, generalized IM and MMM appear to be distinct diseases with different mechanisms. Canine generalized IM may be an important animal model for human IM.

Animals↗

PubMed2006

Denervation hypertrophy may mimic local tumor spread on magnetic resonance imaging.

We report a patient with an extensive paranasal sinus carcinoma. One year after tumor resection, magnetic resonance imaging (MRI) showed swelling of the ipsilateral masticatory muscles with signal increase on T2-weighted images and gadolinium-DTPA uptake, suggestive of local tumor infiltration. However, electromyography, biopsy, and follow-up MRI confirmed denervation pseudohypertrophy of the muscles innervated by the mandibular nerve and excluded tumor recurrence. Muscle denervation and pseudohypertrophy should be considered in the differential diagnosis of appropriate patients with suspected tumor recurrence.

Adult↗

PubMedDate not supplied

Characterization of rabbit masseter muscle fibers.

Myosins of histochemically distinguishable single fibers of rabbit masseter muscle--type 1, 2A, 2B, and slow fibers--have been characterized by gel electrophoresis under dissociating (sodium dodecyl sulfate) and nondissociating (inorganic pyrophosphate) conditions, and by analysis of peptide maps of the heavy chains following limited proteolytic degradation. Type 2B fibers contain more LC3 homodimer than type 2A fibers; peptide maps of their heavy chain are different although the two myosins comigrate on pyrophosphate gel electrophoresis. Slow fiber myosin migrates more slowly than fast myosin and has a distinct peptide map. Differences were also found among fibers of the same histochemical type but originating in different muscles. In adductor magnus 2B myosin the LC1 + LC3 heterodimer band is the strongest, while in masseter 2B myosin the heterodimer is the weakest. Statistical considerations suggest that in masseter there is a mechanism preferentially forming the homodimers. More work is needed to determine the mechanism by which phenotypical differences occur among various fiber types in the same muscle and between corresponding fiber types in different muscles.

Adenosine Triphosphatases↗

PubMed1988

Adult human masseter muscle fibers express myosin isozymes characteristic of development.

Masseter muscle biopsies were obtained from nine patients undergoing orthognatic surgery or surgery for parotid tumors. A detailed enzyme-histochemical and immunocytochemical study of these muscles was performed using antibodies specific to the various isozymes of the myosin heavy chain (MHC) in order to identify the MHC isozymes that were present in the different fiber types. The contractile proteins in these same biopsies were analyzed by two-dimensional gel electrophoresis, native pyrophosphate gel electrophoresis, and by an immunopolypeptide mapping approach. These studies have shown that there is a very heterogeneous distribution of the myosin isozymes, with many fibers containing more than one myosin type. We also present evidence that in addition to adult fast and slow myosin, the human masseter muscle contains two proteins, neonatal MHC and embryonic myosin light chain, that are characteristic of developing muscle.

Electrophoresis↗

PubMed1990

The estimation of motor unit twitch tensions in the human masseter muscle by spike-triggered averaging.

Spike-triggered averaging (STA) has been used to extract twitch profiles of single motor units (SMU) within the human masseter muscle. However, the reported twitch tensions may have been biased by the voluntary firing frequency of the SMUs, the complex architecture of the muscle, and by the biomechanical linkage of the jaw. In this study, a rigid STA paradigm was used to record spike-triggered "measured tensions" (STMTs) for 32 SMUs in the masseter muscles of four subjects. STMTs were recorded at two different orientations of a force transducer placed between the incisor teeth. The STMTs produced by each unit were used to calculate jaw torque. STMTs were also recorded in 11 units with differing degrees of muscle coactivation. STMTs for each unit varied according to the orientation of the force transducer. However, no systematic changes in STMTs occurred with reciprocal changes in the jaw moment arm. STMTs could be altered significantly by different degrees of muscle co-activation. The use of STA as a method for determining SMU tension in the human masseter muscle appears to be highly task-dependent and in the presence of co-activation may be inappropriate.

Action Potentials↗

PubMed1987

Reflex responses of motor units in human masseter muscle to electrical stimulation of the lip.

The reflex responses of single motor units in human masseter muscle to electrical stimulation of the lip were recorded. The subject maintained a content mean level of pre-stimulus excitation of the parent motor neurone by biting in such a way that the unit fired at either 10 or 15 Hz during each trial. When firing at 10 Hz, most units were reflexly inhibited for up to 90 ms by electrical stimuli at intensities that were perceived to be mildly uncomfortable. In many units, the inhibition consisted of 2 phases which were separated from each other by a few spikes occurring about 30 ms after the stimulus. It was occasionally possible to evoke only the latter phase (latency about 40 ms) with stimulus at intensities near the response threshold. In these instances, the inhibitory response became biphasic at higher stimulus intensities with the emergence of a shorter (10-15 ms) component. Still higher intensities caused the 2 phases of inhibition to merge, giving the appearance of a single, prolonged, inhibitory response. When the pre-stimulus firing frequency of the unit was changed from 10 Hz to 15 Hz, the inhibitory responses to the same stimuli were decreased, with the longer-latency component usually surviving beyond the shorter-latency phase. The pattern of reflex responses observed can be explained by a model based on information derived from intracellular recordings in animal experiments.

Action Potentials↗

PubMed1987

Decomposition of the human electromyogramme in an inhibitory reflex.

The activity of up to 4 motor units was recorded simultaneously with electrodes placed in the masseter muscle in human subjects. Mildly noxious electrical shocks were applied to the ipsilateral lip while one of the units was kept firing at a steady frequency by voluntary control. The pattern of reflex responses of each of the units was determined, and spike-triggered averaging was used to measure the potential that each action potential contributed to the rectified surface electromyogramme (EMG). Finally, the average contribution made to the surface EMG by each unit throughout the whole course of the reflex was determined. The contribution of each unit to the reflex response in the surface EMG was found to depend on its firing frequency throughout the course of the response, and on its amplitude measured at the surface. The timing of the various phases of inhibition and activation of different units depended on their pre-stimulus firing frequency. In a given bite, the lowest-threshold units were more likely to be firing most rapidly, and these were least susceptible to the inhibitory stimulus. Higher-threshold units tended to produce larger potentials at the muscle surface but, because they fired more slowly in a given bite, they were more powerfully inhibited by the stimulus. Most units showed the same general pattern of inhibitory and excitatory activity that can be seen in the surface EMG. However, the timing of these various phases in the surface EMG did not necessarily correspond with the timing of inhibition and activation in the records of individual units.(ABSTRACT TRUNCATED AT 250 WORDS)

Action Potentials↗

PubMed1986

Cortically and lingually induced postsynaptic potentials in trigeminal motoneurons after axotomy.

The membrane properties and the efficacy of excitatory and inhibitory synapses were studied in cat masseteric motoneurons (Mass Mns) after axotomy. In axotomized Mass Mns the slope of the primary range in the frequency-current relationship showed a higher gain than that of normal Mass Mns. The safety of antidromic invasion was increased and the initial segment component of antidromic action potentials could not be separated from the soma-dendritic component. In normal Mass Mns a single shock delivered to the orbital gyrus or the lingual nerve induced long-lasting inhibitory postsynaptic potentials (IPSPs). In two-thirds of Mass Mns explored 30 days after axotomy, a single shock delivered to the orbital gyrus or the lingual nerve evoked a mixture of inhibitory and excitatory synaptic potentials. In Mass Mns 50 days after axotomy, we have demonstrated that the major fraction of the total sample of explored Mass Mns showed long-lasting excitatory postsynaptic potentials followed by IPSPs. The results suggest that in Mass Mns, axotomy is followed by the decline of synaptic efficacy of inhibitory rather than of excitatory synapses.

Animals↗

PubMed1989

Non-motoneurons in the facial and motor trigeminal nuclei projecting to the cerebellar flocculus in the cat. A fluorescent double-labelling and WGA-HRP study.

The cerebellar projection from the facial and motor trigeminal nuclei was studied in the cat by means of retrograde axonal transport of wheat germ agglutinin-horseradish peroxidase and fluorescent tracers. The feline facial nucleus was cytoarchitectonically subdivided into ventromedial, ventrolateral, lateral, dorsal, intermediate and medial divisions (see Papez 1927), and the motor trigeminal nucleus into medial, ventral, intermediate, lateral and dorsal divisions. The neurons in the facial and motor trigeminal nuclei were classified as small (ovoid to round cells with a maximum diameter of the cell body of about 20 microns) or large (polygonal to round cells with maximum diameter of about 40 microns). After floccular injections of the wheat germ agglutinin-horseradish peroxidase complex, retrogradely labelled cells were found throughout the facial nucleus, but especially in its medial and dorsal divisions. In the motor trigeminal nucleus, labelled neurons were found only in the ventral, intermediate and lateral divisions. Cases with tracer deposition (implants or injections) in other parts of the cerebellar cortex or nuclei were all negative. All facial and motor trigeminal neurons labelled after floccular injections were smaller than the neurons labelled after injections in the facial mimic and masticatory muscles, and only single-labelled neurons were found following floccular injections of Fluoro-Gold and muscular injections of rhodamine-B-isothiocyanate in the same animals. These observations strongly suggest that the neurons in the facial and motor trigeminal nuclei which project to flocculus are of the non-motoneuron type.

Animals↗

PubMed1989

The masseter inhibitory reflex is evoked by innocuous stimuli and mediated by A beta afferent fibres.

Mechanical or electrical stimulations in the area of the mouth evoke two phases of inhibition in the masseter muscle (early and late inhibitory reflex, also called masseter silent periods). The question whether the afferents of the human masseter inhibitory reflex are nociceptive or non-nociceptive has not yet been settled. We showed that an innocuous stimulus, such as a fine jet of saline directed to the lips of healthy humans, evokes an early and a late masseter inhibitory reflex, similar to those following electrical stimulation. We measured the efferent and afferent delay of the masseter early inhibitory reflex in patients submitted to intracranial stimulation of the motor and sensory trigeminal root, and found that the reflex afferents belong to the intermediately-fast conducting fibre group.

Afferent Pathways↗