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PubMed1992

A five-factor model for motor, psychomotor, and visual-spatial tests used in the neuropsychological assessment of children.

Previous confirmatory factor analysis has supported a distinction between simple and complex motor skill tests in a modified and expanded Halstead Reitan test battery (HRB). The present study used a sample of 722 right-handed boys and girls, aged 9 through 12, and expanded the sample of motor, psychomotor, and visual-spatial tests to further clarify this distinction. Restricted maximum-likelihood factor analysis resulted in correlated factors of Simple Motor Skill, Complex Visual-Spatial Relations, Simple Spatial Motor Operations, Motor Steadiness, and Speeded Motor Sequencing. These results provide additional evidence for the discriminant validity of this particular battery of tests, and explicate further the skills and abilities measured in neuropsychological assessments of children referred for evaluation.

Adolescent↗

PubMed1986

The Wellcome Foundation lecture, 1984. Nuclear magnetic resonance imaging in medicine: medical and biological applications and problems.

From early biological work and the first T1 nuclear magnetic resonance (n.m.r.) animal image in 1974, whole-body patient images, by using a two-dimensional Fourier transform method were achieved in Aberdeen in 1980 with a 0.04 T vertical resistive magnet. Different pulse sequences produce images dependent by different amounts on proton density, T1 and T2, and for clinical work it is advantageous to use more than one pulse sequence to image pathology. The slow improvement of spatial resolution with increasing standing magnetic field strength is discussed and information on the T1 and T2 contrast dependence is reviewed: it suggests that the gains from high fields may be less than believed hitherto. Electrocardiogram gating can be used to produce moving images of the beating heart; blood flow can be imaged and surface radiofrequency coils are used for improved detail. N.m.r. imaging has considerable potential for studying response to therapy; mental states and dementia; tissue generation; discriminating body fat and body fluids. Other nuclei such as 23Na can be imaged and the potential to image fluorine-labelled pharmaceuticals could be very exciting; n.m.r. contrast agents are now being developed. Images formed from T1 values measured for each pixel are very useful for diagnosis, but the numerical values themselves are less valuable for distinctive pathological identification. With 15 companies manufacturing n.m.r. imagers and over 200 in use in hospitals, the technique is rapidly becoming established in diagnostic clinical practice and some typical uses are presented.

Adenocarcinoma↗

PubMed1989

P-element-mediated enhancer detection: a versatile method to study development in Drosophila.

We generated and characterized greater than 500 Drosophila strains that carry single copies of a novel P-element enhancer detector. In the majority of the strains, the beta-galactosidase reporter gene in the P-transposon responds to nearby transcriptional regulatory sequences in the genome. A remarkable diversity of spatially and temporally regulated staining patterns is observed in embryos carrying different insertions. We selected numerous strains as markers for different embryonic organs, tissues, and cells. Many of these strains should allow the study of complex developmental processes, such as nervous system development, which have not been convenient to analyze previously. Also, we present genetic evidence that some of the detected regulatory elements control nearby Drosophila genes. In light of our results, we discuss the diversity and complexity of cis-acting regulatory elements in the genome and the general applications of the enhancer detector method for the study of Drosophila development.

Animals↗

PubMed2002

Spatially "chaotic" solutions in reaction-convection models and their bifurcations to moving waves.

The emergence of stationary spatially multiperiodic or even spatially chaotic patterns is analyzed for a simple model of convection, reaction, and conduction in a cross-flow reactor. Spatial patterns emerge much like dynamic temporal patterns in a mixed system of the same kinetics. Moving waves are formed in an unbounded system but they are transformed into stationary spatially inhomogeneous patterns in a bounded system. The sequence of period doubling bifurcations is determined numerically. The incorporation of a slow nondiffusing inhibitor leads to chaotic spatiotemporal patterns.

Journal Article↗

PubMed1999

Protein structural topology: Automated analysis and diagrammatic representation.

The topology of a protein structure is a highly simplified description of its fold including only the sequence of secondary structure elements, and their relative spatial positions and approximate orientations. This information can be embodied in a two-dimensional diagram of protein topology, called a TOPS cartoon. These cartoons are useful for the understanding of particular folds and making comparisons between folds. Here we describe a new algorithm for the production of TOPS cartoons, which is more robust than those previously available, and has a much higher success rate. This algorithm has been used to produce a database of protein topology cartoons that covers most of the data bank of known protein structures.

Algorithms↗

PubMed1977

Mapping of 23-S rRNA at the ribosomal peptidyl-transferase center by photo-affinity labeling.

Photo-sensitive peptidyl-tRNA's were used to scan the environment of the peptidyl-transferase center of the ribosome. The specificity of the previously described labeling in the 18-S fragment of 23-S rRNA by Boc-Phe(N3)-Phe-tRNA (4-azido-N-t-butoxycarbonyl-phenylalanyl-phenylalanyl-tRNA) was demonstrated by the ability of the covalently anchored molecule to serve as donor substrate in peptide bond formation. Labeling patterns were also obtained with Boc-Phe(N3)-Phe-Phe-tRNA bound at the acceptor site and with Boc-Phe(N3)-(Gly)n-Phe-tRNA (n = 2,4). The results indicate that subsequences within the 18-S fragment of 23-S rRNA are located close to the acceptor site as well as along the path where the peptide moiety adheres to the ribosome. Identification of the labeled sequences is expected to shed light on the spatial arrangement as well as functional role of rRNA in the peptidyl transferase center.

Acyltransferases↗

PubMed1992

Biomagnetic multi-channel systems. Principles and application in cardiology.

The non-invasive measurement of the extremely weak magnetic fields generated by heart and brain is motivated by the possibility of obtaining quantitative diagnostic information about electric function. Magnetic signals (MCG, MEG) are significantly less influenced by body tissue than the corresponding electric signals (ECG, EEG). Measurement of biomagnetic signals is performed by superconducting sensors, consisting of pickup coils and SQUIDs (superconducting quantum interference device) operating in liquid Helium. For clinical investigations a biomagnetic multi-channel system (KRENIKONR) has been designed. It uses a flat array of 37 magnetic field sensors and is operated inside a shielded room. Evaluation of biomagnetic signals by use of simple source and body models and in combination with anatomical data from 3D MR- or CT-images yields sequences of locations of electrical function with a spatial resolution of some millimeters and a time resolution better than one millisecond. More than three years of clinical studies have demonstrated the value of the method primarily in cases with localized functional pathologies. In cardiology this is pathologies of the cardiac conductive pathway, ectopies, and arrhythmias. Validation has been performed by catheter stimulation in volunteers, and by catheter mapping and nuclear medical methods in patients. Extension of modelling and evaluation to cases with distributed activity, e.g. ventricular excitation, is under investigation.

Electromagnetic Fields↗

PubMed2007

Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse.

Ras-associated binding (Rab) protein 3A is a neuronal guanosine triphosphate (GTP)-binding protein that binds synaptic vesicles and regulates synaptic transmission. A mouse mutant, earlybird (Ebd), with a point mutation in the GTP-binding domain of Rab3A (D77G), exhibits anomalies in circadian behavior and homeostatic response to sleep loss. Here, we show that the D77G substitution in the Ebd allele causes reduced GTP and GDP binding, whereas GTPase activity remains intact, leading to reduced protein levels of both Rab3A and rabphilin3A. Expression profiling of the cortex and hippocampus of Ebd and Rab3a-deficient mice revealed subtle differences between wild-type and mutant mice. Although mice were backcrossed for three generations to a C57BL/6J background, the most robust changes at the transcriptional level between Rab3a(-/-) and Rab3a(+/+) mice were represented by genes from the 129/Sv-derived chromosomal region surrounding the Rab3a gene. These results showed that differences in genetic background have a stronger effect on gene expression than the mutations in the Rab3a gene. In behavioral tests, the Ebd/Ebd mice showed a more pronounced mutant phenotype than the null mice; Ebd/Ebd have reduced anxiety-like behavior in the elevated zero-maze test, reduced response to stress in the forced swim test and a deficit in cued fear conditioning (FC), whereas Rab3a(-/-) showed only a deficit in cued FC. Our data implicate Rab3A in learning and memory as well as in the regulation of emotion. A combination of forward and reverse genetics has provided multiple alleles of the Rab3a gene; our studies illustrate the power and complexities of the parallel analysis of these alleles at the biochemical, molecular and behavioral levels.

Adaptor Proteins, Signal Transducing↗

PubMed2004

Coronary MR angiography with steady-state free precession: individually adapted breath-hold technique versus free-breathing technique.

PURPOSE: To compare image quality and coronary artery stenosis detection with breath-hold (BH) and free-breathing navigator-gated (NAV) coronary magnetic resonance (MR) angiography performed with the same imaging sequence (steady-state free precession) and identical spatial resolution in patients suspected of having coronary artery disease. MATERIALS AND METHODS: Forty consecutive patients suspected of having coronary artery disease underwent steady-state free precession MR imaging of the left or the right coronary artery twice. Correction of breathing motion was performed once with NAV and again with BH. Maximal BH duration and coronary artery rest period were individually determined, and duration of data acquisition was adapted (parallel imaging with different sensitivity encoding factors was used). Quantitative analysis of coronary MR angiography data was performed with multiplanar reformatting software to determine visual score for image quality, vessel sharpness, visible vessel length, and number of visible side branches. Diagnostic accuracy for detection of coronary stenosis of 50% or greater was determined in comparison with results of conventional invasive angiography. The two techniques were compared regarding differences in angiographic parameters with paired Student t testing. chi(2) or Fisher exact testing was used when appropriate. RESULTS: More coronary artery segments were assessable with NAV than with BH MR angiography (254 [79.4%] vs 143 [44.7%] of 320 segments). Overall sensitivity and specificity with NAV were 72% (26 of 36 segments) and 91.7% (200 of 218 segments), versus 63% (12 of 19 segments) and 82.3% (102 of 124 segments) with BH; NAV enabled correct diagnosis in 13% more segments. BH yielded nondiagnostic images in 14 patients, while NAV yielded diagnostic images in all patients. When these 14 patients were excluded, there was a significant increase in visual score for left (3.0 vs 2.4, P <.01) and right (3.3 vs 3.0, P <.05) coronary arteries and no significant difference in vessel sharpness but significant improvement in visible vessel length in left coronary artery (85.9 vs 71.4 mm, P =.003) and number of visible side branches in left (4.9 vs 3.9, P =.04) and right (2.8 vs 2.4, P =.04) coronary arteries on NAV images as compared with BH images. CONCLUSION: Free-breathing NAV was superior to BH coronary MR angiography in terms of image quality and diagnostic accuracy of stenosis detection.

Adult↗

PubMed2005

T2-prepared steady-state free precession blood oxygen level-dependent MR imaging of myocardial perfusion in a dog stenosis model.

PURPOSE: To assess the ability of a T2-prepared steady-state free precession blood oxygen level-dependent (BOLD) magnetic resonance (MR) imaging sequence to depict changes in myocardial perfusion during stress testing in a dog stenosis model. MATERIALS AND METHODS: Study was approved by the institutional Animal Care and Use Committee. A hydraulic occluder was placed in the left circumflex coronary artery (LCX) in 10 dogs. Adenosine was administered intravenously to increase coronary blood flow, and stenosis was achieved in the LCX with the occluder. A T2-prepared two-dimensional steady-state free precession sequence was used for BOLD imaging at a spatial resolution of 1.5 x 1.2 x 5.0 mm3, and first-pass perfusion images were acquired for visual comparison. Microspheres were injected to provide regional perfusion information. Mixed-effect regression analysis was performed to assess normalized MR signal intensity ratios and microsphere-measured perfusion differences. For the same data, 95% prediction intervals were calculated to determine the smallest perfusion change detectable. Means +/- standard deviations were calculated for myocardial regional comparison data. A two-tailed Student t test was used to determine if significant differences (P < .01) existed between different myocardial regions. RESULTS: Under maximal adenosine stress, MR clearly depicted stenotic regions and showed regional signal differences between the left anterior descending coronary artery (LAD)-fed myocardium and the stenosed LCX-fed myocardium. Visual comparisons with first-pass images were also excellent. Regional MR signal intensity differences between LAD and LCX-fed myocardium (1.24 +/- 0.08) were significantly different (P < .01) from differences between LAD and septal-fed myocardium (1.02 +/- 0.07), which was in agreement with microsphere-measured flow differences (LAD/LCX, 3.38 +/- 0.83; LAD/septal, 1.26 +/- 0.49). The linear mixed-effect regression model showed good correlation (R = 0.79) between MR differences and microsphere-measured flow differences. CONCLUSION: On T2-prepared steady-state free precession BOLD MR images in dogs, signal intensity differences were linearly related to flow differences in myocardium, with a high degree of correlation. SUPPLEMENTAL MATERIAL: radiology.rsnajnls.org/cgi/content/full/236/2/503/DC1

Animals↗

PubMed2006

Evolution of cognitive deficits and conversion to dementia in patients with mild cognitive impairment: a very-long-term follow-up study.

Ten patients with mild cognitive impairment (MCI) underwent extensive neuropsychological evaluation at 12-monthly intervals for a minimum of 6 years. All 10 patients declined and 5 have now died. The onset of dementia, as defined by a fall in global cognitive function (MMSE <24) or activities of daily living (Clinical Dementia Rating Scale; CDR) ranged from 1 to 8 years with generally good concordance between these measures. The rate of decline on the MMSE was highly variable ranging from 0.86 to 2.83 points per year. Other than a consistent impairment on tests of episodic memory and category fluency (8 out of 10), other early cognitive deficits were difficult to define and tended to be unstable in the early stages. Impairment of semantic memory, visuo-spatial and attentional abilities eventually developed but the sequence of deficit acquisition was heterogeneous. These findings are discussed in the light of current views of MCI. Amnestic MCI may not be an accurate concept unless semantic memory impairment is also considered as an integral core deficit. Full-blown dementia may take many years to develop in patients with MCI but was a universal feature in this study.

Activities of Daily Living↗

PubMed2026

Identification of cryosensitive niches and a targetable FOS/AP‑1 program in the human ovarian cortex by single‑cell and spatial transcriptomics.

BACKGROUND: The ovary is a vital and dynamic reproductive organ. Ovarian tissue cryopreservation (OTC) plays a vital role in preserving female fertility. However, the cellular subtypes most susceptible to cryoinjury and the molecular mechanisms underlying cryopreservation-associated damage remain poorly understood. This study aimed to identify cell populations vulnerable to freezing-thawing and to elucidate the key transcriptomic alterations and signaling pathways associated with ovarian cryoinjury at the single-cell and spatial levels. METHODS: Ovarian cortical tissues from patients undergoing three gender reassignment surgery (GRS) were divided into fresh and vitrification-rapid warming groups. Following collagenase IV digestion, 10x Genomics single-cell RNA-seq was used for dissociated ovarian cell suspensions (27,185 fresh and 25,480 frozen-thawed cells). Eight major cell clusters were identified. Additionally, 110 oocytes (66 fresh, 44 vitrification-rapid warming) were isolated and analyzed using the Smart-seq2 platform. Spatial transcriptomics was performed via BGI Stereo-seq. Molecular validation was performed via β-galactosidase staining, immunofluorescence, and qRT-PCR. RESULTS: Cryopreservation significantly altered the activity of pathways related to focal adhesion, oxidative stress, and apoptosis, particularly in stromal and perivascular cells. The number of FOS-positive perivascular cells was notably increased after vitrification-rapid warming, whereas the number of PTGDS-positive stromal cells decreased. Oocyte analysis revealed that cryopreservation primarily disrupted pathways involved in the cell cycle and meiosis, although the damage was not irreversible, supporting the relative safety of long-term cryostorage. Spatial transcriptomics and functional validation further confirmed the rapid and robust activation of the FOS/AP-1 pathway after vitrification-rapid warming, particularly in perivascular and granulosa cells. Treatment with T-5224 (a FOS/AP-1 inhibitor) significantly rescued the morphology and function of cultured frozen-thawed ovaries. CONCLUSIONS: Stromal and perivascular cells are the main cell types that are sensitive to ovarian cryopreservation. The FOS/AP-1 pathway is markedly activated after, suggesting the exacerbation of metabolic impairment. In oocytes within the ovarian cortex, the cell cycle and meiosis-related physiological processes were the primary processes affected.

Female↗

PubMed2004

Temporal transformation of periodic incoherent ultrashort light pulses by chirped fiber gratings.

The analogy between free-space propagation of optical beams and light-pulse reflection from linearly chirped fiber gratings is used to analyze the Lau effect in the temporal domain. The coherence conditions that are satisfied in the spatial domain for obtaining, at certain fixed locations, periodic fringes patterns are reformulated for guided light propagation. In this analogy, spatial periodic irradiance distributions are transformed in periodic sequences of light pulses. An optical setup is proposed to produce sharp pulse trains, with minimal distortion effects, that have repetition frequencies that are different from those associated with the input periodic optical signal. Some numerical results are given to illustrate this approach.

Journal Article↗

PubMed2005

Neural coupling binds visual tokens to moving stimuli.

Spatially separated visual objects that appear in alternating sequence can be perceived as a single moving object. This phenomenon of apparent motion enables us to perceive sequentially presented images as a motion picture. How does the visual system bind together single visual tokens to one moving object? Here we report a series of experiments investigating apparent motion with electroencephalographic recordings showing that gamma-band oscillatory coupling in the visual cortex is crucial for this phenomenon. We used an ambiguous stimulus that could be perceived as moving either vertically or horizontally. Because visual information from the right and left visual hemifields is routed to the contralateral early visual cortex, in the case of perceived horizontal movement, information from both hemispheres has to be integrated. In accord with this assumption, our data show stronger oscillatory coupling between right and left visual cortices during perception of horizontal motion compared with vertical motion.

Adult↗

PubMed1994

Vascular neoplasms and malformations, ischemia, and hemorrhage affecting the spinal cord: MR imaging findings.

This essay illustrates the imaging spectrum of vascular neoplasms and malformations, ischemia, and hemorrhage affecting the spinal cord. Most of these abnormalities occur far more frequently in the brain. The goal of this essay is to provide a sound anatomic and radiologic basis for the evaluation of these diseases. Knowledge of the unique cross-sectional anatomy, blood supply, and venous drainage of the spinal cord is essential in understanding the radiographic appearance of certain vascular lesions. The superior spatial resolution of fast spin-echo pulse sequences and improvements in MR angiography have expanded the role of MR imaging in the diagnosis of spinal cord diseases. These techniques are emphasized in this essay, and potential diagnostic pitfalls are highlighted. The diseases illustrated are grouped in the categories of enlarged vessels, ischemia, and hemorrhage.

Adult↗

PubMed2004

The multi-item localization (MILO) task: measuring the spatiotemporal context of vision for action.

We describe a new multi-item localization task that can be used to probe the temporal and spatial contexts of search-like behaviors. A sequence of four target letters (e.g., E, F, G, and H) was presented among four distractor letters. Observers located the targets in order. Both retrospective and prospective components of performance were examined. The retrospective component was assessed by having target items either vanish or remain once they had been located. This manipulation had little effect on search performance, suggesting that old target items can be efficiently ignored. The prospective component was assessed by shuffling future target and distractor locations after each response. This manipulation revealed that observers typically plan ahead at least one target into the future. However, even when observers cannot plan ahead, they are still able to ignore old targets. These findings suggest that both "what you did" and "what you intend to do" can influence the localization and selection of targets.

Adult↗

PubMed1991

Learning disability in rural primary school children.

In rural primary school children observed for two years, 12.97 per cent of those having IQ greater than or equal to 90 were found to have poor achievement in arithmetic test and teacher's assessment. These learning disabled children had impaired perceptual maturity and conceptual grasp as observed on MISIC (Indian modification of WISC), Bender Gestalt test and Piaget's test. On WISC Bannatyne categories learning disabled children scored highest in verbal conceptualization (similarities, vocabulary, comprehension), followed by spatial (picture completion, object assembly, block design) and sequencing (arithmetic, digit span, coding) abilities. These children on Bender Gestalt test made more errors particularly distortions (distortion of parts, incorrect number of dots, shape of design lost etc). They also showed delayed development on Piagetian tasks class inclusion, conservation (for length, substance, liquid and number) ordinal relation and one to one correspondence. These observations indicate impaired perceptual maturity, conception and information processing deficit.

Achievement↗

PubMed1985

[NMR tomography of excised human lymph nodes].

The paper is concerned with the potentialities of the NMR-tomography technique (1H) for metastructure studying of the biopsied human lymph nodes both uninvolved and affected by tumor. Tomography was performed at 200 MHz using the Bruker CXP-200t mini-tomograph. Spin-echoes sequence and projection-reconstruction technique was employed. Spatial resolution was about 0.1 mm. Images of the uninvolved lymph nodes permitted one to detect the structure of the lymph nodes. NMR-imaging of unenlarged lymph nodes enabled one to localize the region of initial metastases. The reconstruction of various groups of spin-echo signals permitted a more precise detection of the regions differed in their spin-spin magnetic relaxation times T2 of protons in 1 mm slice. It may be assumed that the distinction in T2-relaxation rates of the normal and abnormal tissues displays an effective means to detect a pathological region using high-field tomography.

Adult↗