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PubMed2003

Haplotypic QTL mapping in an outbred pedigree.

An offspring genome can be viewed as a mosaic of chromosomal segments or haplotypes contributed by multiple founders in any quantitative trait locus (QTL) detection study but tracing these is especially complex to achieve for outbred pedigrees. QTL haplotypes can be traced from offspring back to individual founders in outbred pedigrees by combining founder-origin probabilities with fully informative flanking markers. This haplotypic method was illustrated for QTL detection using a three-generation pedigree for a woody perennial plant, Pinus taeda L. Growth rate was estimated using height measurements from ages 2 to 10 years. Using simulated and actual datasets, power of the experimental design was shown to be efficient for detecting QTLs of large effect. Using interval mapping and fully informative markers, a large QTL accounting for 11.3% of the phenotypic variance in the growth rate was detected. This same QTL was expressed at all ages for height, accounting for 7.9-12.2% of the phenotypic variance. A mixed-model inheritance was more appropriate for describing genetic architecture of growth curves in P. taeda than a strictly polygenic model. The positive QTL haplotype was traced from the offspring to its contributing founder, GP3, then the haplotypic phase for GP3 was determined by assaying haploid megagametophytes. The positive QTL haplotype was a recombinant haplotype contributed by GP3. This study illustrates the combined power of fully informative flanking markers and founder origin probabilities for (1) estimating QTL haplotype magnitude, (2) tracing founder origin and (3) determining haplotypic transmission frequency.

Haplotypes↗

PubMed2006

Effects of four tanniferous plant extracts on the in vitro exsheathment of third-stage larvae of parasitic nematodes.

The anthelmintic properties of tanniferous plants and of their secondary metabolites represent one possible alternative to chemotherapy that is currently being explored as a means of achieving sustainable control of gastrointestinal nematodes in ruminants. Previous in vivo and in vitro results suggest that tanniferous plants can have direct anti-parasitic effect against different stages of nematodes. However, the mode of action of the bioactive plant compounds remains obscure. The objectives of the current study were (1) to examine the hypothesis that extracts of tanniferous plants might interfere with the exsheathment of third-stage infective larvae (L3); (2) to assess the role of tannins in the process by examining the consequence of adding an inhibitor of tannins (polyethylene glycol: PEG) to extracts. The effects of 4 tanniferous plant extracts on exsheathment have been examined on L3 of Haemonchus contortus and Trichostrongylus colubriformis. Artificial exsheathment was induced in vitro by adding hypochloride solution to larval suspension. The evolution of exsheathment with time was measured by repeated observations at 10-min interval for 60 min. The selected plants were: genista (Sarothamnus scoparius), heather (Erica erigena), pine tree (Pinus sylvestris), and chestnut tree (Castanea sativa), with tannin contents ranging from 1.5 to 24.7% of DM. Extracts of a non-tanniferous plant (rye grass, tannin content: 0.3% of DM) were included in the assay as negative controls. The extracts were tested at the concentration of 600 microg/ml and the effects were compared to the rate of exsheathment of control larvae in PBS. No statistical differences in the pattern of exsheathment was observed after addition of rye grass or genista extracts for both nematode species and with heather extracts for T. colubriformis. In contrast, pine tree extracts on larvae of both species and heather extracts with H. contortus induced a significant delay in exsheathment. Last, contact with chest nut extracts led to a total inhibition of the process for both nematodes. These results suggest that extracts of tanniferous plants might affect a key process in the very early stages of larval invasion of the host. In most cases, the addition of PEG led to a total or partial restoration towards control values. This suggests that tannins are largely involved in the inhibitory process. However, other secondary metabolites may also interfere with the process that would help to explain some of the differences in response observed between the two nematode species.

Animals↗

PubMed2003

Effects of thinning treatment on an ectomycorrhizal succession under Scots pine.

We describe a 16 yr dataset of ectomycorrhizal fruit bodies under Scots pine (Pinus sylvestris), starting from seedlings, and explore the effects of a 50% thinning treatment imposed in year 12. The over-riding pattern in the data was of successional development, with Paxillus involutus and Laccaria proxima in the earliest years, followed by Suillus species, while in later years Amanita and Cortinarius species became prominent. The typical pattern was for each species in turn to increase to a maximum count then gradually decline without actually disappearing from the community (an addition succession). For nine out of the 30 species recorded the time profiles were fitted significantly by a long-normal curve, although the best-fit lognormal models consistently under-estimated the peak count. Thinning increased counts of Suillus bovinus, Gomphidius roseus and Cortinarius semisanguineus, but overall its effect on the community was minor. Rainfall in September was weakly correlated with successional advancement (measured as DCA first axis scores). Mean fruiting date increased significantly as the stands aged.

Colony Count, Microbial↗

PubMed2005

A new Leptographium species associated with Tomicus piniperda infesting pine logs in Korea.

During the course of a survey of sapstaining fungi in Korea, a Leptographium was isolated from Pinus densiflora and P. koraiensis logs, infested with the bark beetle Tomicus piniperda. The fungus grew optimally at 25 C on 2% malt extract agar and showed a high level of tolerance to cycloheximide. The Leptographium has unusually short conidiophores and is morphologically similar to L. pini-densiflorae, L. lundbergii, L. yunnanense, and the Leptographiumn anamorph of Ophiostoma crassivaginatum. Comparisons of DNA sequence data for three gene regions, as well as morphological characteristics, confirmed that this fungus represents an undescribed taxon. We consequently provide the name Leptographium koreanum sp. nov. for it here.

Animals↗

PubMed2004

Characterisation of Ophiostoma species associated with pine bark beetles from Mexico, including O. pulvinisporum sp. nov.

Bark beetles (Coleoptera: Scolytidae) are common vectors of Ophiostoma species. These fungi include primary tree pathogens and important sapstain agents. In Mexico, Ips calligraphus and Dendroctonus mexicanus occur on many species of pine. Pinus maximinoi and P. pseudostrobus are the hosts of both species of insects. Little research has been done on ophiostomatoid fungi associated with pine bark beetles in Mexico. We recently obtained specimens of these bark beetles and their galleries from Mexico. The aim of the study was to isolate and identify Ophiostoma species associated with the two beetle species. In total, six ophiostomatoid species were found to be associated with them. These included Ceratocystiopsis minuta, Ophiostoma pluriannulatum, an O. galeiformis-like species, two unidentified Sporothrix spp., as well as a new species similar to O. adjuncti, O. ips, and O. montium, that we name as O. pulvinisporum sp. nov.

Animals↗

PubMed2005

Leptographium longiclavatum sp. nov., a new species associated with the mountain pine beetle, Dendroctonus ponderosae.

The mountain pine beetle, Dendroctonus ponderosae, and its fungal associates are devastating the lodgepole pine forests in British Columbia, Canada. During our fungal survey, an unknown Leptographium species has been consistently isolated from both D. ponderosae and infested lodgepole pine (Pinus contorta var. latifolia). This Leptographium species has similar morphology with the Leptographium anamorph of Ophiostoma clavigerum whose association with the D. ponderosae is well known. However, thorough morphological comparisons showed that this fungus is distinct from all the other Leptographium species described in the literature, and especially from O. clavigerum. Comparison of DNA sequences of multiple loci and the profiles by the PCR-RFLP marker also confirmed that this Leptographium species represents an undescribed taxon. Based on its distinct morphological, physiological characteristics and phylogenetic position, we describe it as L. longiclavatum sp. nov.

Actins↗

PubMed2003

Orographic cold-trapping of persistent organic pollutants by vegetation in mountains of western Canada.

Conifer needles from mountain areas of Alberta and British Columbia, Canada, were collected from sites that ranged in altitude from 770 to 2200 masl and were analyzed for polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCs) to determine if they are progressively concentrated in colder, more elevated mountain areas, where temperatures decrease as elevation increases. Concentrations of OCs in needles ranged from 43 to 2430 pg g(-1), 55-17500 pg g(-1), and 11-2930 pg g(-1) (dry weight), for total hexachlorocyclohexanes (HCHs), PCBs, and endosulfans, respectively. The more volatile OCs, with subcooled liquid vapor pressures (PL) > 0.1 Pa at 25 degrees C, increased at higher altitudes, whereas the less volatile OCs were either unrelated or inversely correlated with altitude. These spatial patterns were similar for species of spruce (Picea engelmannii and glauca) and pine (Pinus contorta and albicaulis). Back trajectories revealed that air masses arriving at these sites traveled over Asia and the Pacific Ocean before reaching the Rocky Mountains. Results from this study demonstrate that alpine ecosystems accumulate these chemicals to the same degree that is observed in polar environments that are known to receive contaminants by long-range transport.

Air Movements↗

PubMed2002

Low nitrogen losses with a new source of nitrogen for cultivation of conifer seedlings.

Losses of nitrogen (N) when cultivating plants may cause a number of adverse environmental effects. N losses from conifer nurseries, for instance, may have a considerable impact on the local environment, and studies indicate that the bulk of added N is not recovered in the cultivated plants. This study was conducted to obtain insight into the causes of the low recovery and to test an alternative N fertilizer. Hence, growth of the economically important Scots pine (Pinus sylvestris (L).) seedlings and the recovery of different forms of added nitrogen (N) were investigated in a greenhouse experiment. Containerized seedlings were grown in peat for one summer, with two different N fertilizers, one organic (arginine) and one inorganic (a commercial fertilizer (CF) containing a mixture of ammonium and nitrate) each at an N concentration of 3 mM. At the end of the growth period, some seedlings were labeled with solutions containing either U-[13C6], [15N4]-arginine, (15NH4)2SO4, or K15NO3 supplied to the growth substrate. Labeled seedlings were harvested 1 h, 5 days, and 19 days after tracer addition, and the recovery of each added nitrogen source in both the seedlings and the growth substrate was measured. The retention of the three N forms during discharge of solutions from the growth substrate, peat, was tested in a separate experiment. Arginine-fed seedlings grew better and had higher needle N concentrations than the CF-fed seedlings. Isotopic data showed that the arginine treatment gave significantly higher N recoveries (80%) compared to the CF treatment (50%). The low recovery of N in the CF treatment was largely due to very low recovery (30%) of NO3- -N. The retention of the different N forms during discharge of solutions from the growth substrate was highest for arginine, somewhat lower for NH44+, and very low for NO3-. The high rate of seedling growth and the small nitrogen losses observed when using arginine suggest that this amino acid may be an efficient and environmentally favorable N source for cultivating conifer seedlings.

Arginine↗

PubMed2003

Reconstructing tritium exposure using tree rings at Lawrence Berkeley National Laboratory, California.

Annual tritium exposures were reconstructed using tree cores from Pinus jeffreyi and Eucalyptus globulus near a tritiated water vapor release stack. Both tritium (3H) and carbon-14 (14C) from the wood were measured from milligram samples using accelerator mass spectrometry. Because the annual nature of the eucalyptus tree rings was in doubt, 14C measurements provided growth rates used to estimate the age for 3H determinations. A 30-yr comparison of organically bound tritium (OBT) levels to reported 3H release data is achieved using OBT measurements from three trees nearthe stack. The annual average 3H, determined from atmospheric water vapor monitoring stations, is comparable to the OBT in proximal trees. For situations without adequate historical monitoring data, this measurement-based historical assessment provides the only independent means of assessing exposure as compared to fate and transport models that require prior knowledge of environmental conditions and 3H discharge patterns.

Environmental Exposure↗

PubMed2002

Acaricidal activity of pine essential oils and their main components against Tyrophagus putrescentiae, a stored food mite.

Some essential oils obtained from the branches of four Pinus species (P. pinea L., P. halepensis Mill., P. pinaster Soil in Ait., and P. nigra Arnold) have been evaluated for their acaricidal activity by aerial diffusion against the stored food mite Tyrophagus putrescentiae (L.). All the essential oils showed a good efficacy, but P. pinea oil and its two constituents 1,8-cineole and limonene were the most effective compounds, showing 100% acaricidal activity at 8 microL; 1,8-cineole showed the same activity at 6 microL.

Acyclic Monoterpenes↗

PubMed2005

Antioxidative activity of volatile extracts isolated from Angelica tenuissimae roots, peppermint leaves, pine needles, and sweet flag leaves.

Volatile extracts were isolated from dried medicinal plants [Angelica tenuissimae roots (AT, Angelica tenuissima Nakai), peppermint leaves (PL, Mentha arvensis L.), pine needles (PN, Pinus sylvestris L.), and sweet flag leaves (SF, Acorus gramineus Rhizoma)] using steam distillation under reduced pressure, followed by continuous liquid-liquid extraction (DRP-LLE). The extracts were then analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The major volatile constituents of AT, PL, PN, and SF were 3-butylidene-4,5-dihydrophthalide (32 mg/g), menthol (18 mg/g), thunbergol (2.1 mg/g), and cis-asarone (37 mg/g), respectively. The inhibitory activity (%) of the extracts against hexanal oxidation ranged from 33 to 98% at a level of 50 microg/mL. Among the volatile extracts, PL and PN increased cell viabilities by 10 and 24%, respectively, at a dose of 1 microg/mL, compared to that of H2O2-treated brain neuroblastoma cells, SK-N-SH. However, a 20% reduction in the malonaldehyde level, an index for lipid peroxidation, was observed at only 1 microg/mL concentration of PN.

Acorus↗

PubMed2006

Estimation of wood density and chemical composition by means of diffuse reflectance mid-infrared Fourier transform (DRIFT-MIR) spectroscopy.

Sitka spruce (Picea sitchensis) samples (491) from 50 different clones as well as 24 different tropical hardwoods and 20 Scots pine (Pinus sylvestris) samples were used to construct diffuse reflectance mid-infrared Fourier transform (DRIFT-MIR) based partial least squares (PLS) calibrations on lignin, cellulose, and wood resin contents and densities. Calibrations for density, lignin, and cellulose were established for all wood species combined into one data set as well as for the separate Sitka spruce data set. Relationships between wood resin and MIR data were constructed for the Sitka spruce data set as well as the combined Scots pine and Sitka spruce data sets. Calibrations containing only five wavenumbers instead of spectral ranges 4000-2800 and 1800-700 cm(-1) were also established. In addition, chemical factors contributing to wood density were studied. Chemical composition and density assessed from DRIFT-MIR calibrations had R2 and Q2 values in the ranges of 0.6-0.9 and 0.6-0.8, respectively. The PLS models gave residual mean squares error of prediction (RMSEP) values of 1.6-1.9, 2.8-3.7, and 0.4 for lignin, cellulose, and wood resin contents, respectively. Density test sets had RMSEP values ranging from 50 to 56. Reduced amount of wavenumbers can be utilized to predict the chemical composition and density of a wood, which should allow measurements of these properties using a hand-held device. MIR spectral data indicated that low-density samples had somewhat higher lignin contents than high-density samples. Correspondingly, high-density samples contained slightly more polysaccharides than low-density samples. This observation was consistent with the wet chemical data.

Cellulose↗

PubMed1999

Differential expression of genes encoding the light-dependent and light-independent enzymes for protochlorophyllide reduction during development in loblolly pine.

The expression patterns of the two distinct subfamilies of genes (designated porA and porB) encoding the light-dependent NADPH:protochlorophyllide oxidoreductases (PORs) in loblolly pine (Pinus taeda L.) were examined. Transcripts arising from both gene subfamilies were shown to be present at high levels in the cotyledons of dark-grown pine seedlings and to a lesser extent in their stems. Exposure of dark-grown seedlings to light resulted in increased levels of both porA and porB transcripts, as well as increased levels of mRNAs encoding other photosynthesis-related gene products, suggesting that they are under a common mode of regulation. Relative levels of the porA and porB transcripts were similar in seedling cotyledons and primary needles of two-month-old pine trees, whereas only porB transcripts were present at a significant level in mature secondary needles of two-year-old trees. Immunoblot analysis showed that the 37 kDa PORA protein was most abundant in dark-grown tissues, decreased dramatically upon exposure to light, but could still be detected at low levels in light-grown seedlings. In comparison, levels of the 38 kDa PORB protein were not significantly changed upon transfer of dark-grown tissues to light. While both PORA and PORB were detected in cotyledons and primary needles, only PORB could be detected in mature needles. Transcripts derived from the three plastid genes, chlL, chlN, and chlB, encoding subunits of the light-independent protochlorophyllide reductase were detected in the cotyledons and stems of dark-grown seedlings, and in mature needles. The highest levels of chlL, chlN, and chlB transcripts were detected within the top one-third of the stem and decreased gradually towards the stem/root transition zone. Correspondingly, the highest levels of light-independent chlorophyll formation took place near the top of the hypocotyl. A similar pattern of expression was observed for other photosynthesis-related gene products, including porA and porB. Our results suggest that many aspects of the light-dependent, tissue-specific and developmental regulation of POR expression first described in angiosperms were already established in the less evolutionarily advanced gymnosperms. However, unlike angiosperms, light is not the dominant regulatory factor controlling porA expression in these species.

Algal Proteins↗

PubMed2001

Behavioral and electrophysiological responses of Arhopalus tristis to burnt pine and other stimuli.

The exotic longhorn beetle Arhopalus tristis is a pest of pines, particularly those damaged by fire, and a major export quarantine issue in New Zealand. Actinograph recordings of caged individuals showed that males and females were most active from dusk to midnight. Olfactometer experiments indicated that females moved upwind toward odors from burnt pine (80%, N = 75), compared to unburnt pine (20%). Oviposition choice tests showed that eggs were predominantly laid on burnt logs (79%, N = 20), compared to unburnt logs. Beetles were trapped by funnel traps baited with burnt (mean catch per trap 7.8) and unburnt (mean catch 4.1 per trap) pine bark from inside a screen cage (4 x 3 m), while unbaited traps had a mean catch 0.1 beetles (N = 8 replicates). The treatment of burnt pine bark with a 1:1:2 mixture of green leaf volatiles (E)-2-hexen-1-ol and (E)-2-hexenal) in mineral oil as a repellent reduced trap catch by fivefold in a similar experiment (mean catches of 1.2 beetles per trap to burnt pine bark plus repellent treatment and 6.2 beetles per trap to burnt pine bark alone). The treatment of burnt pine bark with this solution also reduced oviposition by 98.5% (mean eggs per log of 11.1 on burnt pine and 0.3 on burnt pine plus repellent), indicating that oviposition cues have the potential to be significantly disrupted. The electrophysiological responses of adult beetles were recorded to a range of odorants. Normalized responses to monoterpenes known to occur in Pinus radiata ranged from about 20 to about 150, with alpha-terpineol giving the greatest responses in both sexes. Green leaf volatiles also gave high responses. The potential exists to improve the management of this insect using chemical cues in various ways.

Animals↗

PubMed2002

Comparing the variation of needle and wood terpenoids in Scots pine provenances.

We determined variation in both the concentration and composition of terpenoids in needles and wood within nine Scots pine (Pinus sylvestris) provenances. Seedlings of different provenances representing a 1200-km N-S transect from Estonia to northern Finland were cultivated in Suonenjoki nursery field, central Finland, for seven years. Growth of seedlings and the number of vertical resin ducts in wood were also determined. alpha-Pinene and 3-carene were the major monoterpenes both in the needles and wood. The total monoterpene concentration was about five times higher in the needles than in the wood. A strong positive correlation was found between proportional quantities of several terpenes of the needles and wood, particularly for 3-carene, sabinene, and terpinolene. The needles contained both labdane-type and tricyclic resin acids, whereas the wood contained only tricyclic ones. The wood had a four times higher tricyclic resin acid concentration than the needles. The highest total monoterpene concentration in the needles and in the wood occurred in the most northern Muonio provenance and in the most southern Saaremaa provenance plants, respectively. The amount of high 3-carene genotype trees decreased among the northern provenances. The wood of the most northern Muonio provenance showed the lowest total resin acid concentration, but provenance did not affect total tricyclic resin acids in the needles. Korpilahti provenance trees from central Finland had the best growth in height. In addition, Korpilahti and Ruokolahti provenance trees showed largest radial growth of stem and smallest number of vertical resin ducts. The results suggest that especially the proportional quantity of 3-carene in the needles could be used in estimating the amount of this compound in the wood and vice versa.

Genotype↗

PubMed2002

Influence of odor from wood-decaying fungi on host selection behavior of deathwatch beetle, Xestobium rufovillosum.

Adult females of Xestobium rufovillosum de Geer demonstrated anemotactic orientation when exposed to an odor plume containing volatiles generated by wood-decaying fungi (Coriolus versicolor, Donkioporia expansa) and decayed oak wood (Quercus petraea, Quercus robur). They did not orient towards undecayed oak wood, beech (Fagus sylvatica), or pine wood (Pinus sylvestris). Although all insects tested showed anemotactic orientation, responses were nonlinear with respect to insect age. Adult females more readily oriented upwind when they were between 10 and 16 days old. Oviposition choice bioassays showed that ovipositing females would preferentially oviposit on extract-treated cellulose paper discs that had been treated with various strains of the wood-decaying fungus, Donkioporia expansa. HPLC-fractionated mycelial extracts were attractive to ovipositing deathwatch beetles, whereas HPLC-fractionated fungal broth extracts were repellent to ovipositing females. The implications of these results are discussed in the context of timber pest management in historically important buildings.

Animals↗

PubMed2002

Tree-ring analysis for the assessment of anthropogenic changes and trends.

Investigation of Pine (Pinus sylvestris L.) annual radial increment (width of annual tree rings) was carried out in the surroundings of one of the largest pollution sources in Lithuania - Jonava Nitrogen Fertilizers Plant. The main objective of investigation was to analyse different sides of anthropogenic transformations of tree-ring series in the polluted environment: changes in tree growth intensity: variance changes in tree-ring series: changes in the relations with natural external factors. Three different periods of tree reaction to the environmental pollution were singled out - fertilization period, depression period and recovery period since annual emissions were essentially reduced. The variance of tree-ring series has increased several times in the polluted environment. Reaction of trees to the impact of climatic factors (temperature, precipitation) has changed significantly in the polluted environment and their sensitivity has also increased.

Air Pollutants↗

PubMed2001

Pycnogenol for diabetic retinopathy. A review.

Diabetic retinopathy represents a serious health threat to a rapidly growing number of patients with diabetes mellitus. The retinal microangiopathy is characterised by vascular lesions with exudate deposits and haemorrhages causing vision loss. Pycnogenol, a standardised extract of the bark of the French maritime pine (Pinus pinaster), is known to increase capillary resistance. Pycnogenol has been tested for treatment and prevention of retinopathy in five clinical trials with a total number of 1289 patients since the late 1960's. All but one of these studies have been reported in French and German and, today, are of limited accessibility, giving the impetus for reviewing them in detail in this article. There were two open case studies and two double blind studies (one controlled against calcium dobesilate and another against placebo) and, finally, one multi-center field study with 1169 diabetics. All of these studies unequivocally showed that Pycnogenol retains progression of retinopathy and partly recovers visual acuity. Treatment efficacy of Pycnogenol was at least as good as that of calcium dobesilate. Pycnogenol was shown to improve capillary resistance and reduce leakages into the retina. Tolerance was generally very good and side effects were rare, mostly referring to gastric discomfort. In conclusion, treatment with Pycnogenol had a favourable outcome in the majority of the patients with diabetic retinopathy.

Diabetic Retinopathy↗