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At least 145 records · Page 8Linked to original sources
PubMed2006

Strategies for evaluating B*18 allelic diversity by sequence-based typing applied to studies of a population from Singapore and African-Americans.

Strategies to resolve B*18 alleles which carry a deletion in intron 1 close to the 5' end of exon 2 relative to other HLA-B alleles or a null allele mutation in exon 1 and to resolve ambiguities among allele combinations including B*18 are described. B*18 allele frequencies from volunteer donors recruited for two hematopoietic stem cell registries show the presence of two alleles, B*180101 and B*1802, in a population from Singapore and only B*180101 in African-Americans.

Black or African American↗
PubMed2006

Routine HLA sequence-specific-oligonucleotide typing and confirming sequence-based typing of a prospective bone marrow donor identified a novel B*27 allele, HLA-B*2734.

In this study, we report the identification of a new human leukocyte antigen-B allele in a sample that was tested in our routine typing for volunteer bone marrow donors. This novel allele officially named B*2734 was found in a female donor of Caucasoid origin (laboratory code 158567). The search for unrelated bone marrow donors was initiated by the Aktion Knochenmarkspende Bayern. In comparison with the closest related allele B*2707, B*2734 differs at two nucleotide positions, all located in exon 3, at position 412, 'A' in B*2707 is replaced by 'G' and at position 486, 'G' is replaced by 'A' in the novel allele. The single nucleotide polymorphism at position 412 results in an amino acid substitution at codon 138. The amino acid asparagine is changed to aspartic acid in B*2734 allele. The second nucleotide exchange is a silent mutation and codon 163 remains unaffected as ACG-->ACA, still codes for alanine.

Alleles↗
PubMed2006

A novel HLA-DRB1*11 allele, DRB1*1155, was found in a patient through routine DRB1 typing using sequence-specific primers (SSP) and confirmed by allele-specific sequence-based typing.

In comparison with DRB1*1155 allele, DRB1*1103 differs at position 220/221, 'GC' is changed to 'CT', or DRB1*1125 differs at position 210/211, 'AG' is substituted with 'GA'. This results in a single amino acid exchange depending on the closest related allele investigated, whether DRB*1103 codon 74 alanine (GCG) is changed to leucine (CTG) or DRB1*1125 codon 71 arginine (GAG) is replaced with glutamic acid.

Alleles↗