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At least 145 records · Page 8Linked to original sources
PubMed1992

From paper chromatography to drug discovery: Zaffaroni.

A paper chromatography assay for adrenocortical steroids, published just as cortisone's therapeutic value in arthritis was announced, had far-reaching consequences. Its use by Upjohn scientists led to the first synthesis of cortisone. Interest in the assay also led the author to an association with Syntex S.A. in Mexico, which he was instrumental in transforming from a small chemical company into the large pharmaceutical firm, Syntex Corporation, having its base of operations in the United States. The major events of this history are described, showing how steroid product development helped establish Syntex and later played a role in the founding of ALZA Corporation.

Adrenal Cortex Hormones↗
PubMed1978

Separation of radioactive histamine and some of its metabolites by one-dimensional paper chromatography.

A simple one-dimensional paper chromatograph technique is described for the separation of radioactive histamine and some of its metabolites in blood and tissues. After removal of protein, the supernatant fluid (200 microliter) is subjected to two-stage one-dimensional paper chromatography. This enables large numbers of samples to be chromatographed simultaneously. Using this technique in conjunction with liquid scintillation counting, it was possible to monitor the small amounts (0.02 nCi) of radioactive histamine and its metabolites in serial blood samples.

Animals↗
PubMed1969

Investigation of ketoacidurias by two-dimensional paper chromatography.

A simple paper chromatographic method was found to be effective for the study of phenylketonuria and tyrosyluria. It proved to be much more reliable than conventional tests for the detection of abnormal amounts of ketoacids in urine and was suitable and convenient for following the effects of dietary variations on the excretion of these compounds. Information obtained about ketones other than those of primary importance to the conditions studied included confirmation of the excretion of p-hydroxyphenylpyruvic acid in phenylketonuria. A modification of the method applicable to histidinaemia was devised.

Amino Acid Metabolism, Inborn Errors↗
PubMed1979

Location by paper chromatography of compensatory ovarian hypertrophy (COH) inhibiting activity in isobutanol extracts of bovine pineals.

Bovine pineal glands were extracted according to the methods reported by Bensinger et al. (1973) and Cheesman and Fariss (1970). Isobutanol soluble COH-inhibiting activity was further separated by chromatography on Sephadex G-15 and paper chromatography. With the Bensinger method, different active pineal fractions were obtained from Sephadex G-15 columns. Certain of those fractions were further separated by paper chromatography in butanol : acetic acid : water (4 : 1 : 1) and the COH-inhibitor was localized. The pineal COH-inhibitor could also be localized by high pressure, reverse phase liquid chromatography. More COH-inhibiting activity was extracted with the Bensinger method than with aqueous and acetic acid extraction methods used earlier by us. The Cheesman extraction method for arginine vasotocin gave less regular results in our hands than the Bensinger extraction method.

Animals↗