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A new apparatus for continuous cultivation of bacterial plaque on solid surfaces and human dental enamel.

A new apparatus for the continuous cultivation of mono and mixed bacterial plaque on solid surfaces is described. The features are: easy preparation and handling; freedom from technical problems and microbial contamination; self-sufficient for periods of up to 56 d; 12 samples are taken simultaneously; programmable supply inlet. Experiments were performed with Streptococcus mutans C 67-1 for mono bacterial inoculation and in combination with Veillonella alcalescens V-1 for mixed bacterial inoculations. The results showed that the controlled conditions and versatility of the apparatus make possible the study of plaque-development and lesion production on a time-dependent basis. It is concluded that the apparatus is suitable for a wide range of dental and non-dental applications.

Bacteria↗

Gene expression of growth differentiation factors in the developing periodontium of rat molars.

Growth and differentiation factors (GDF) 5, 6, and 7 are known to play roles in tendon and ligament formation, and are therefore probably involved in the formation of periodontal ligament. In this study, we sought to determine temporal and spatial expression of GDF-5, -6, and -7 mRNA in developing periodontal tissue of rat molars using in situ hybridization. GDF gene expression in the periodontal ligament was first detected in cells associated with the initial process of periodontal ligament fiber bundle formation. Gene signals were also detected in cells located along the alveolar bone and cementum surfaces, the insertion sites of periodontal ligaments, during the course of root formation. GDF expression in these cells were down-regulated after completion of root formation. Our results appeared to suggest the involvement of GDF-5, -6, and -7 in the formation of the dental attachment apparatus.

Alveolar Process↗

[Is connective tissue reattachment on root surfaces exposed to the oral environment possible?].

In advanced periodontal diseases, where the root surface a longer time is exposed to the oral environment, retaining of teeth is only possible through de novo-attachment of the periodontal ligament on the diseased root surface. Even when surgical reconstruction of the alveolar bone is successful, connective tissue fibers fail to attach to the affected dental cementum. Patients with two- and three wall pockets can be successfully treated by Nymans method of guided tissue regeneration. However the restoration of the dental attachment apparatus in cases with advanced horizontal destruction of the alveolar bone is still an unsolved problem of periodontal therapy. Investigations of an artificial attachment support will be presented. A synthetic felt glued onto the root surface with special dental adhesives shall obtain the necessary support reattachment of the connective tissue fibers at the periodontitis affect root.

Guided Tissue Regeneration, Periodontal↗

Dental amalgam--environmental aspects.

Increasing knowledge about the risk of toxic effects caused by anthropogenic mercury accumulation in ecosystems has resulted in a growing pressure for reduction of the discharge of mercury waste. Consequently, the mercury waste problems of dental clinics have been given increased attention, and restrictions on handling and discharge of contaminated waste have been established in several countries. Major amalgam particles from trituration surplus of those produced during the carving and burnishing of new amalgam restorations are generally collected in coarse filters and sold for refinement. Minor amalgam particles released by production of new fillings or by removal of old restorations partly sediment in tubes and drains. The remaining particles are carried with the waste water stream to the local purifying plant. In Scandinavia, the industrial discharge of mercury-contaminated waste water has been reduced to a minimum. According to recent investigations, dental clinics appear to be responsible for the major amount of mercury collected in the sludge generated in purifying plants. If threshold values for heavy metal content, including mercury, are exceeded, the sludge is not allowed to be recycled as fertilizer. Installation of an approved amalgam-separating apparatus in dental clinics is now mandatory in several countries--for example, Switzerland, Germany, Sweden, and Denmark. Approval of amalgam separators is based on national testing programs, including clinical or laboratory tests demanding 95-99% separating efficiency.

Biological Availability↗

The mercury burden in waste water released from dental clinics.

The aim of this study was to estimate mercury burden in waste water samples collected during one working day in 27 dental clinics. The samples were subjected to authorized analysis using the technique of QS-IE Enhanced Cold Vapour Mercury Analyzer to estimate the amount of mercury discharged. The mean value of mercury discharged was 2.49 parts per billion (ppb) for the clinics equipped with amalgam separators and 94.75 ppb for the clinics without amalgam separators. Some of the small amalgam particles released when new fillings are placed or during removal of old restorations form a sediment in tubes and drains. The remaining particles are carried with the waste water stream to the local purifying plant. If threshold values for heavy metal content including mercury are exceeded, the sludge is not allowed to be recycled as fertiliser. Installation of an approved amalgam-separating apparatus in dental clinics is recommended so as to reduce considerably the discharge of mercury into waste water.

Dental Clinics↗

Comparison of the surface detail reproduction of flexible die material systems.

STATEMENT OF PROBLEM: Flexible die materials have been introduced to facilitate the fabrication of indirect provisional or definitive restorations in 1 appointment. PURPOSE: This study compared the surface detail reproduction of 7 potential flexible die materials when used in combination with 7 elastomeric impression materials. METHODS: Surface detail reproduction of 17 impression material/flexible die material systems was compared with a control system containing an elastomeric impression material and a Type IV dental stone. Test dies of each system were prepared in a random order with the American Dental Association apparatus for testing detail reproduction, compatibility, and dimensional stability. RESULTS: One flexible die system had better surface detail reproduction than the control stone die, other systems had similar or worse reproduction. Surface detail reproduction was adversely affected when a separator was required between the impression and flexible die material. CONCLUSIONS: Impregum F die material with Extrude Light impression material produced better surface detail reproduction than the control dies. Impregum F impressions were incompatible with Blu-Mousse, Impregum F, or Imprint die materials. Polyvinyl siloxane impressions were incompatible with polyvinyl siloxane dies unless a separator was used. When a separator was used, the surface detail reproduction was not as good as the control die system or the Extrude Light impression material/Impregum F die material combination.

Analysis of Variance↗