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[PulseNet Japan: surveillance system for the early detection of diffuse outbreak based on the molecular epidemiological method].

As the foods are stocked below freezing and widely distributed, a kind of food-borne outbreak which occurs in separate regions or in different time, so called "diffuse outbreak", has been found at the present day. Unless the outbreak is early recognized, the number of victims would increase. Some methods have been developed to analyze the relatedness of bacteria isolated from the patients of enteric infections. PFGE, pulsed-field gel electrophoresis, is one of the methods and powerful to discriminate the difference in nucleotide sequences among bacterial genomes. Availability of PFGE analysis is appreciated to examine the linkage of each incident of food-borne infections in epidemiological investigation. A PFGE network, PulseNet Japan, is now under construction among National Institute of Infectious Diseases, local Health Institutes and Ministry of Health, Labour and Welfare.

DNA, Bacterial↗

Applications of epidemiologic methods to podiatric medicine.

National statistics show that the burden of occupational foot problems is large. For example, over 20 per cent of work-related injuries occur in the lower extremities. However, to date few epidemiologic studies have been undertaken to document the nature, frequency, and causes of occupational foot problems. More are clearly needed. The results of such studies could profitably be disseminated among occupational health professionals to increase their awareness of this relatively neglected aspect of worker health.

Epidemiologic Methods↗

[Socioeconomic status and coronary disease: theories, research methods, epidemiological evidence and the results of Italian studies].

This review covers the most important theoretic contributions and results of epidemiological research on the relationship between socio-economic status (SES) and coronary heart disease (CHD). Two major approaches have been identified: those of the British and American schools. In spite of differences in method, most of published contributions have shown social inequalities in the outcome of most degenerative disorders. Similar patterns have been detected for the CHD: the time trend declines documented in recent years in most industrialized countries has not filled in the gap of SES difference in the incidence and mortality for CHD, which even seems to have increased in the lower social classes. Moreover, an apparent paradox has been described: even though CHD is typical of welfare societies, its impact is higher in the lower social classes. Recent results of Italian studies are reported which investigated the relationship between CHD risk factors and SES in representative samples. The findings suggest the presence, in Italy, too, of social discrepancies in the prevalence of major risk factors, in both gender groups. This should be taken in consideration when preventive programmes are planned in populations or in work settings.

Adult↗