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Comparison of hemisphere size in wild and domestic geese.

The hemisphere size has been studied in wild and domestic geese (Anser fabalis, n = 77: A. albifrons, n = 48; A. anser, n = 8; A. anser f. domestica, n = 10), which were classified according to particular age category (adult or immature). Material used in studies was fixed in 4% solution of formaldehyde. The brain weight as well as hemispheres' length, width and height was measured with standard method. Morphometric characteristic of geese's brains has been presented in absolute and relative way (by parallel use of index and allometric approach). The appropriate ontogenic, taxonomic and domestication comparisons were carried out. The greatest differences referred to absolute and relative width and height of hemispheres.

Animals↗

Hepatic cirrhosis suggesting aflatoxicosis in a flock of geese.

An outbreak of hepatic cirrhosis in a flock of young geese was investigated. The affected geese were cyanotic with purple beaks, shanks and footwebs. On post mortem examination severe atrophy of the liver was seen and, histologically, extensive areas of necrosis, cirrhosis and bile duct proliferation were prominent. Liver function tests and serum enzyme levels confirmed that the liver damage was extensive. The findings are compared with those found in aflatoxicosis of other avian species.

Animals↗

Regional cerebral blood flow and tissue oxygenation during hypocarbia in geese.

Very low arterial CO2 tension (PaCO2) experienced by birds during high-altitude flight may result in cerebral vasoconstriction and reduced cerebral O2 delivery. To examine this possibility, we measured regional cerebral blood flow (CBF) and tissue PO2 in pentobarbital-anesthetized geese (Anser domesticus). Twenty-five-micrometer Teflon-coated platinum electrodes for H2-clearance measurements of local blood flow or tissue PO2 were implanted in the cerebral cortex in 11 geese. Tissue H2 and O2 were measured by voltage clamping the electrodes at +0.30 and -0.5 V, respectively. Washout kinetics of H2 gas administered via unidirectional lung ventilation was used to calculate local blood flow for those electrodes exhibiting one- or two-compartment washout kinetics of H2 (128 of 296 washouts in 31 electrodes). PaCO2 was controlled between 8 and 55 mmHg by altering pulmonary gas flow or by adjusting inspired PCO2. CBF decreased as PaCO2 fell from 50 to 20 mmHg but did not decrease further as PaCO2 was reduced below 20 mmHg. CBF was uniformly distributed in different regions of the cortex. Despite the plateau in CBF during severe hypocapnia, tissue PO2 continued to decline as PaCO2 fell below 20 mmHg. Severe alkalosis may limit cerebral O2 delivery in birds during high-altitude flight.

Animals↗

Mutant hemoglobins (alpha 119-Ala and beta 55-Ser): functions related to high-altitude respiration in geese.

The unusually high blood-O2 affinity in the bar-headed and Andean geese is a necessary adaptation for migration across high mountain ranges. The amino acid residues alpha-119 and beta-55, which form an alpha 1 beta 1 contact in human hemoglobin (Hb), are altered in bar-headed and Andean geese, respectively, which suggests that loss of this contact increases O2 affinity. Two mutant human Hbs with equivalent mutations at these sites prepared by site-directed mutagenesis show the same increase in O2 affinity compared with Hb A, which indicates that these mutations are responsible for the changes in the protein. The intrinsic affinity difference compared with native Hb A is amplified by organic phosphates. Whereas the recombinant and native Hbs displayed similar sensitivities to pH, chloride, and 2,3-diphosphoglycerate, the oxygenation heat of the alpha-chain mutant decreased in the presence of 2,3-diphosphoglycerate. O2 association constants for the deoxygenated state of the alpha-mutant were about three times those for Hb A. The mutant Hb analogously exhibited higher affinity constants for binding the first three O2 molecules. Calculated heme-heme interaction energies indicated that loss of a single contact, resulting in destabilization of the deoxy (tense) structure, underlies the increased O2 affinity. Adaptations securing Hb-O2 binding at extreme altitude are discussed.

2,3-Diphosphoglycerate↗

Identification of a novel bile acid in swans, tree ducks, and geese: 3alpha,7alpha,15alpha-trihydroxy-5beta-cholan-24-oic acid.

By HPLC, a taurine-conjugated bile acid with a retention time different from that of taurocholate was found to be present in the bile of the black-necked swan, Cygnus melanocoryphus. The bile acid was isolated and its structure, established by (1)H and (13)C NMR and mass spectrometry, was that of the taurine N-acyl amidate of 3alpha,7alpha,15alpha-trihydroxy-5beta-cholan-24-oic acid. The compound was shown to have chromatographic and spectroscopic properties that were identical to those of the taurine conjugate of authentic 3alpha,7alpha,15alpha-trihydroxy-5beta-cholan-24-oic acid, previously synthesized by us from ursodeoxycholic acid. By HPLC, the taurine conjugate of 3alpha,7alpha,15alpha-trihydroxy-5beta-cholan-24-oic acid was found to be present in 6 of 6 species in the subfamily Dendrocygninae (tree ducks) and in 10 of 13 species in the subfamily Anserinae (swans and geese) but not in other subfamilies in the Anatidae family. It was also not present in species from the other two families of the order Anseriformes. 3alpha,7alpha,15alpha-Trihydroxy-5beta-cholan-24-oic acid is a new primary bile acid that is present in the biliary bile acids of swans, tree ducks, and geese and may be termed 15alpha-hydroxy-chenodeoxycholic acid.

Animals↗

Sampling effort/frequency necessary to infer individual acute stress responses from fecal analysis in Greylag geese (Anser anser).

Measuring hormone metabolites from excreta is a powerful method to study hormone-behavior relationships. Currently, fecal corticosterone metabolite concentrations are used to estimate individual short-term stress responses. From the free-roaming, semitame flock of greylag geese (Anser anser), as many fecal samples as possible were collected over 3 h following a challenge (social density stress) or in a control situation. This time span corresponds to the gut passage time of geese. It was asked how many samples were necessary to determine differences in excreted corticosterone immunoreactive metabolites (CORTs) between control and social density stress and which parameters (means, maxima, range) reliably showed this difference. A large variation of CORT was found between consecutive samples. Still, means, maxima, and ranges of the samples in a fecal series consistently showed the response to a stressor both within and between individuals. Three samples sufficed if the maximum value of CORT was used, whereas four or more samples were necessary to work with the mean. It was concluded that by increasing the number of fecal samples collected, the course of CORT could be measured more precisely and an individual's acute stress response inferred more reliably.

Animals↗

Comparative pathological studies on domestic geese (Anser anser domestica) and Muscovy ducks (Cairina moschata) experimentally infected with parvovirus strains of goose and Muscovy duck origin.

Parvovirus infection of Muscovy ducks caused by a genetically and antigenically distinct virus has been reported from Germany, France, Israel, Hungary, some Asian countries and the USA. The pathological changes include those of degenerative skeletal muscle myopathy and myocarditis, hepatitis, sciatic neuritis and polioencephalomyelitis. In the study presented here, day-old and 3-week-old goslings and Muscovy ducks were infected experimentally with three different parvovirus strains (isolates of D-216/4 from the classical form of Derzsy's disease, D-190/3 from the enteric form of Derzsy's disease, and strain FM from the parvovirus disease of Muscovy ducks). All three parvovirus strains caused severe disease in both day-old and 3-week-old Muscovy ducks but in the goslings only the two strains of goose origin (D-216/4 and D-190/3) caused disease with high (90-100%) mortality when infection was performed at day old. Strain FM (of Muscovy duck origin) did not cause any clinical signs or pathological lesions in the goslings. In the day-old goslings and Muscovy ducks the principal pathological lesions were severe enteritis with necrosis of the epithelial cells (enterocytes) of the mucous membrane and the crypts of Lieberkühn, and the formation of intranuclear inclusion bodies. Other prominent lesions included hepatitis and atrophy (lymphocyte depletion) of the lymphoid organs (bursa of Fabricius, thymus, spleen). In goslings infected with the strain originating from the classical form of Derzsy's disease mild myocarditis was also detected. After infection at three weeks of age, growth retardation, feathering disorders, myocardial lesions (degeneration of cardiac muscle cells, lympho-histiocytic infiltration) and hepatitis were the most prominent lesions in both geese and Muscovy ducks. In addition to the lesions observed in the geese, muscle fibre degeneration, mild sciatic neuritis and polioencephalomyelitis were also observed in the Muscovy ducks infected with any of the three parvovirus strains.

Age Factors↗

Free-living Canada geese and antimicrobial resistance.

We describe antimicrobial resistance among Escherichia coli isolated from free-living Canada Geese in Georgia and North Carolina (USA). Resistance patterns are compared to those reported by the National Antimicrobial Resistance Monitoring System. Canada Geese may be vectors of antimicrobial resistance and resistance genes in agricultural environments.

Animals↗

Comparison of procedures for collecting semen from ganders and inseminating geese.

Experiments were conducted to compare management of ganders and semen collection procedures with respect to semen and sperm yield, and two frequencies of artificial insemination were tested with respect to fertility. Housing ganders in groups, singly, or singly with the introduction of a female just before collection was the rank order of housing system from least to most successful collection of ejaculates. There were no significant differences among types of housing with respect to semen volume, but ejaculates from ganders housed singly had the greatest (P less than .05) spermatozoal concentrations. Ejaculates collected with an artificial vagina were of greater (P less than .05) volume and total spermatozoal yield but not spermatozoal concentration than those collected by aspiration. Interactions between collector and method were observed for spermatozoal traits. Geese inseminated on 2 consecutive days/wk showed greater (P less than .01) fertility than those inseminated once per week. Therefore, collection of semen with an artificial vagina from ganders housed singly, with insemination weekly but on consecutive days, should result in successful reproduction of geese.

Animals↗

Research note: avian leukosis retroviral genes are not detected in geese.

Genomic DNA from four strains of geese was analyzed for the presence of endogenous viral elements using a probe that can detect over 20 Rous-associated endogenous viral genes (ev genes) in chickens, as well as a probe and protocol that detects endogenous avian viruses (EAV). Southern blot analysis of genomic DNA did not reveal any ev genes in DNA of 15 geese from Chinese, Synthetic, or two Embden goose strains. Even under low stringency conditions, using a probe that covered most of the polymerase (pol) gene of the Rous-associated virus (RAV) and that revealed EAV elements in a chicken without ev genes, no viral loci were evident in goose DNA.

Animals↗

An outbreak of schistosomiasis in Atlantic brant geese, Branta bernicla hrota.

A heavy infection with schistosomes of the genera Trichobilharzia and Dendritobilharzia was considered the cause of 90% mortality in a group of 40 wild-caught Atlantic brant geese (Branta bernicla hrota) that were maintained in captivity on a fresh-water pond in Aurora, Ontario. Numerous adult worms and eggs were disseminated in many organs throughout the body of all birds examined. The main pathological findings, attributed to both eggs and adults, included emaciation, thrombosis of the caudal mesenteric vein and its branches, fibrinohemorrhagic colitis, and in some birds, heptomegaly. Translocation of brant geese from their natural marine environment to a fresh-water pond may have caused them to be exposed to parasites which they would not normally encounter.

Animals↗

Multicentric intramuscular lipomatosis/fibromatosis in free-flying white-fronted and Canada geese.

Over a period of 9 yr, seven white-fronted geese (Anser albifrons) and one Canada goose (Branta canadensis) with multiple intramuscular mesenchymal tumors were encountered in Saskatchewan (Canada) and one similarly affected Canada goose was seen on Prince Edward Island (Canada). The tumors in these birds consisted either of adipose tissue, fibroblastic tissue, or a mixture of both types of tissues. The high prevalence of this condition in white-fronted geese suggested a genetic influence.

Animals↗

Prevalence of spindle cell sarcomas among wild Canada geese from southern Illinois.

Over a 6-yr period, two cases of neoplastic disease were diagnosed among 1,272 wild Canada geese (Branta canadensis interior) collected in Illinois (USA), Wisconsin (USA), and Ontario, Canada. One juvenile goose of each sex was involved. In both cases > 35 discrete masses were observed subdermally, intramuscularly, and within the body cavity. The tumors, diagnosed as spindle cell sarcomas, are among the most commonly observed neoplasms in free-living North American waterfowl. Based on our data, we suggest that these neoplasms occur with extremely low prevalence (0.002%) in free-living Canada geese.

Animals↗

Avian cholera in waterfowl: the role of lesser snow and ross's geese as disease carriers in the Playa Lakes Region.

We collected samples from apparently healthy geese in the Playa Lakes Region (USA) during the winters of 2000-01 and 2001-02 to determine whether carriers of Pasteurella multocida, the bacterium that causes avian cholera, were present in wild populations. With the use of methods developed in laboratory challenge trials (Samuel et al., 2003a) and a serotype-specific polymerase chain reaction method for identification of P. multocida serotype 1, we found that a small proportion of 322 wild birds (<5%) were carriers of pathogenic P. multocida. On the basis of serology, an additional group of these birds (<10%) were survivors of recent avian cholera infection. Our results confirm the hypothesis that wild waterfowl are carriers of avian cholera and add support for the hypothesis that wild birds are a reservoir for this disease. In concert with other research, this work indicates that enzootic infection with avian cholera occurs in lesser snow goose (Chen caerulescens caerulescens) populations throughout their annual cycle. Although fewer Ross's geese (Chen rossii) were sampled, we also found these birds were carriers of P. multocida. Even in the absence of disease outbreaks, serologic evidence indicates that chronic disease transmission and recent infection are apparently occurring year-round in these highly gregarious birds and that a small portion of these populations are potential carriers with active infection.

Animals↗

Zoonotic diseases: health aspects of Canadian geese.

OBJECTIVE: Review zoonotic diseases associated with Canadian geese. STUDY DESIGN: Review article: A review of the multiple physical, microbiologic and safety concerns, and methods used in controlling this potential problem. RESULTS: Over the last decade the Canadian goose population (protected by international treaties and protection acts) has increased rapidly such that in many cities they have become a pest rather than an admired wild bird. Their increasing numbers have caused a number of potential healthcare concerns including: physical, bacterial, parasitic, allergic and viral potential problems. The Canadian goose fecal droppings of one per minute have caused falls and the flying geese have caused air traffic accidents. Bacterial concerns, including botulism, salmonella and E. coli have all been reviewed and presented concerns. The viral Newcastle disease may be detected with hemagglutination studies and the Giardia psittaci parasites have been repeatedly found in their droppings. The Cryptosporidium parvum oocytes have been present on stool study. CONCLUSIONS: Definite links to human infectious diseases have been difficult to prove. Revision of the current laws and new control programs must be developed.

Accidental Falls↗

[Influence of the cecum, in geese fed diets containing urea, on N-metabolism and digestibility of nutrients and on urea concentration in excrements and blood].

Balance experiments were performed on six normal young geese and on six geese with a ligated caecum. The ration consisted of 43.25% barley, 30% sugar beet slices, 20% cellulose, 1.4% maize germ oil, 3% mineral mixture, 1% concentrate of vitamins and growth factors, 1.25% urea and 0.1% lysine. Daily nitrogen balance was similar in the two groups (average 1051 mg in the controls and 1090 mg in the experimental birds). Crude protein digestibility was 82.4 +/- 1.1% in the controls and 84.9 +/- 1% in the experimental birds (a significant difference). The bacterial flora of the caecum seemed to have no effect on crude fibre breakdown. Digestibility was quite low, averaging 5.7% in controls and 4.6% in experimental birds. The proportion of urea discharged in excreta was 50% in controls and 55.7% in experimental birds. Blood urea veraged 21.7 mg/100 g in controls and 22.8 mg in experimental birds (insignificant difference).

Animal Feed↗

[Detection of goose haemorrhagic polyomavirus (GHPV) in flocks with haemorrhagic nephritis and enteritis of geese in southern Germany].

Two flocks of geese in the South of Germany independently diseased on Haemorrhagic Nephritis and Enteritis of Geese (HNEG) at the age of 4 weeks. The flocks were approximately 300 km apart but had received goslings from the same hatchery. In both flocks the animals died within 12 hours mainly without showing clinical signs. Some of the first cases showed haemorrhagic typhlitis, whereas in later cases visceral gout was the main finding. In all cases, pathohistological examination generally showed necrosis of the tubular epithelium of the kidney. After a course of 5 weeks no new occurrences were seen. Death rates of 43.8% for the first flock and 29.2% for the second flock, respectively, were recorded. In both cases, the diagnosis HNEG was confirmed by the detection of the recently described Goose Haemorrhagic Polyomavirus (GHPV) using polymerase chain reaction.

Animals↗