GERMINAL CENTER CHANGES AND PLASMA CELL REACTION DURING THE PRIMARY IMMUNE RESPONSE.
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Electrical sizing of liver cell nuclei gives similar results as histological techniques for the mean volume, the volume distribution and the proportions of the different nuclear volume populations. The electrical method has, compared to histological technics, however, the advantage of higher rapidity and accuracy. Due to the good volume resolution of the improved electrical sizing method two populations of nuclei with different mean volumes can be distinguished in rat liver tissue besides di-, tetra- and octoploid cell nuclei. In addition to the volume distribution curves, the mean electrical resistance of isolated liver nuclei can be determined by the electrical sizing method. A newly developed micromehod provides enough cell nuclei for electrical sizing from only 10 mg liver tissue in a few minutes. This should enable the measurement of nuclear volume distribution curves of human biopsy material from the liver and gastrointestinal tract for diagnostic purposes on a larger scale.
The histobiopsy of the bone marrow is more and more included in clinicohaematological diagnostics. It has better diagnostic possibilities than the cytology of smears (aspiration biopsy). The specimen is taken from the iliac crest by puncture or the milling method. The sample can be considered representative of the whole bone marrow with regard to the normal quantity and quality. The pathologist ordinarily per forms the processing and assessment of the specimens in a desirably constructive cooperation with the clinical haematologist. The paper presents a condensed review of the histological technics and the recording of features in bone marrow sections, the indications for bone marrow histobiopsy, the results (2769 cases of literature and 496 own cases), and the clinical significance of human bone marrow histobiopsy.
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