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At least 145 records · Page 8Linked to original sources

Combined transcript and metabolite analysis reveals genes involved in spider mite induced volatile formation in cucumber plants.

Many plants have an indirect defense against herbivores by emitting volatiles that attract carnivorous enemies of the herbivores. In cucumber (Cucumis sativus) the production of carnivore attractants can be induced by herbivory or jasmonic acid spraying. From the leaves of cucumber plants with and without spider mite infestation, two subtractive cDNA libraries were made that were enriched in cDNA fragments up- or down-regulated by spider mite infestation. A total of 713 randomly selected clones from these libraries were used to make a cDNA microarray. Subsequently, cucumber plants were sprayed with jasmonic acid, mechanically damaged, infested with spider mites, or left untreated (control). Leaf samples were taken at a range of different time points, and induced volatile compounds and mRNA (from the same leaves) were collected. cDNAs prepared from the mRNA were hybridized to the clones on the microarray. The resulting gene expression profiles were analyzed in combination with volatile production data in order to gain insight in the possible involvement of the studied genes in the synthesis of those volatiles. The clones on the microarray and the induced cucumber volatiles could be grouped into a number of clusters in which specific biosynthetic genes clustered with the product of that pathway. For example, lipoxygenase cDNA clones clustered with the volatile (Z)-3-hexenyl acetate and the volatile sesquiterpene (E,E)- alpha-farnesene clustered with an up-regulated sesquiterpene synthase fragment. This fragment was used to screen a cDNA library which resulted in the cloning of the cucumber (E,E)-alpha-farnesene and (E)-beta-caryophyllene synthases. The use of combined global gene expression analysis and metabolite analysis for the discovery of genes involved in specific biosynthetic processes is discussed.

Amino Acid Sequence↗

A case report of Cheyletiella infestation on a Whippet dog in Korea.

A clinical case of Cheyletiella infestation on a dog born and raised in Korea is reported. A three-year old female Whippet was hospitalized due to a multiple fracture and displacement of the left scapula caused by a recent car accident. The mite infestation was not noticed at the time of hospitalization. The dog underwent multiple operations involving internal fixation of the fractured scapula with wire and a plate, followed by extensive chemotherapy with antibiotics and prednisolone. After two months of hospitalization, a pruritic dermatitis near the left scapula developed. Multiple white dandruff-like flakes were seen on the hair coat, especially over the dorsal spine and neck, and the dog expressed increased pruritus by frequently licking and scratching the affected areas. Local dense accumulations of skin debris that became crusty were also observed. Microscopic examination of a skin scraping revealed a heavy infestation of cheyletiella yasguri, as identified by the presence of hoks of the palpi and the heart-shaped sensory organ on genu I. Immunosuppression elicited by the extensive administration of prednisolone was suspected for the initiation of the generalized mite infestation.

Animals↗

A nonchemical method of controlling the northern fowl mite, Ornithonyssus sulviarum (Canestrini and Fanzago), on caged White Leghorn hens.

Populations of the northern fowl mite on hens but not on roosters were significantly reduced relative to normally feathered chickens when feathers in the vent area were clipped to 2 to 3 mm length. The average difference in egg production between the control (no mites) and the clipped mite-infested hens was only 2.6% during the 22-week study, but the average difference between the control and the unclipped mite-infested hens was 7.6%, which was significantly different (P less than .01). Egg weight, hen body weight, and feed consumption of the three groups of hens were not significantly different.

Animals↗

Eradication of ear mites from naturally infested conventional research rabbits using ivermectin.

Rabbits naturally infested with ear mites were treated with ivermectin injection for cattle, subcutaneously at the rate of 400 mcg/kg; which was repeated in 15 to 17 days. Rabbits treated as described and housed in a conventional vivarium environment were found to be free of mites during a subsequent 33 to 139 day observation period. Side effects were minimal and associated with occasional transient discomfort at the injection site. Ivermectin appears to be safe and effective for treating rabbits with ear mites. The prospects of eradicating mites from infested rabbit colonies using this method of treatment is promising.

Animals↗

Effect of ivermectin on murine mites.

Two SC injections of ivermectin (200 micrograms/kg of body weight), given one week apart, safely and effectively eliminated mite infestations (Myocoptes musculinus and Myobia musculi) from laboratory mice. When one 200 micrograms/kg or one 100 micrograms/kg injection of ivermectin was given to the mice, mite infestations were reduced temporarily.

Animals↗

Russian honey bee (Hymenoptera: Apidae) colonies: Acarapis woodi (Acari: Tarsonemidae) infestations and overwintering survival.

Honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies infested by parasitic mites are more prone to suffer from a variety of stresses, including cold temperature. We evaluated the overwintering ability of candidate breeder lines of Russian honey bees, most of which are resistant to both Varroa destructor Anderson & Trueman and Acarapis woodi (Rennie), during 1999-2001. Our results indicate that Russian honey bee colonies (headed by original and supersedure queens) can successfully overwinter in the north, even during adverse weather conditions, owing to their frugal use of food stores and their resistance to tracheal mite infestations. In contrast, colonies of Italian honey bees consumed more food, had more mites, and lost more adult bees than Russian honey bees, even during unusually mild winter conditions.

Animals↗

An infestation of the mite Sancassania berlesei (Acari: Acaridae) in the external auditory canal of a Korean man.

We here report the case of a storage mite, Sancassania berlesei, infestation in the external auditory canal of a 46-yr-old male. He complained of feeling a foreign body and itching in the left external auditory canal for 1 mo, with accompanying otalgia for 3 days. Considering the duration of the patient's complaint and the 8-9-day life cycle of the mite, the mites are believed to have lived in the patient's ear for more than 3 generations.

Animals↗

Establishment of Demodex canis on canine skin engrafted onto scid-beige mice.

A small animal model of canine demodicosis is described. Normal canine skin was engrafted onto scid (severe combined immunodeficient)-beige mice, which lack functional B and T lymphocytes and have reduced natural killer cell activity. The xenografts were later infected with Demodex canis collected from a dog with demodicosis. At 30-112 days following infection, mites were seen histologically in the canine hair follicles of the engrafted skin. Demodex canis adults, nymphs, larvae, and eggs were present in samples macerated in sodium hydroxide. Mite infestations could not be demonstrated in the mouse skin, nor were mites passed from the infected graft to uninfected skin grafts on in-contact mice. This model may be utilized to assess the efficacy of miticidal treatments, to evaluate the importance of specific components of the immune response, and to study the biology of D. canis.

Animals↗

A comparison of mite populations in mattress dust from hospital and from private houses in Cardiff, Wales.

Fifty mattress dust samples from private houses were examined--all were mite infested. Dermatophagoides pteronyssinus occurred in all samples and accounted for 81% of all mites. In thirty-two paired samples the total mite population of bedroom floor dust was only 10% of that found in the corresponding mattress dust. D. pteronyssinus was the dominant species in both and the population of this mite amounted to 7-5% of that found in the mattress dust. An analysis of mattress dust from 100 hospital beds showed that ninety-four mattresses were mite free; the other six contained only seven mites, all D. pteronyssinus. It is suggested that frequent changing and washing of bed linen and brushing and cleaning of mattresses were the main factors in preventing mite infestation in the Cardiff hospitals.

Bedding and Linens↗

Efficacy of topical eprinomectin in the treatment of Chorioptes sp. infestation in alpacas and llamas.

Chorioptes sp. mite infestation is increasingly recognized as a cause of skin disease in New World camelids and there is a need for an effective treatment protocol to eliminate herd infestation. In this field trial, eprinomectin applied topically at the rate of 0.5 mg kg(-1) weekly for 10 weeks was found to be ineffective in a herd of 12 llamas and 16 alpacas.

Administration, Cutaneous↗

Host specificity of Laelaps (Acari: Laelapidae) in central Brazil.

A study of associations between small mammals and ectoparasites in two adjacent nature reserves near Brasilia, Federal District, Brazil, revealed that ectoparasitic mites of the genus Laelaps Koch are host specific. Data on the prevalence and intensity of mite infestations were combined with measures of the reproductive activity of mite populations to estimate the association of each host-mite pair. Twelve morphologically distinct species of Laelaps occurred in monoxenous and oligoxenous associations with the cricetid rodent genera Oryzomys, Nectomys, Rhipidomys, and Calomys. Nine were referred to species recognized at the time of the study; three were described as new species in a recent publication. When mites initially assigned as the same species infested more than one host species, an examination of the morphological variation within and among mite specimens supported the hypothesis that mites infesting different hosts were reproductively isolated populations. Dispersal pathways apparently were restricted, occurring primarily between conspecific individuals. In one instance, two morphologically similar groups of Laelaps paulistanensis Fonseca were associated with two closely related rodent species, Oryzomys fornesi Massoia and O. nigripes (Olfers). Although the data presented here indicate that these two groups of mites are conspecific, further study is warranted.

Animals↗

Control of the straw itch mite (Acari: Pyemotidae) with sulfur in an insect rearing facility.

The ectoparasitic mite Pyemotes tritici (Lagrèze-Fossat & Montané) (Acari: Pyemotidae) caused paralysis and reduced longevity in eucalyptus longhorned borer, Phoracantha semipunctata F., under laboratory rearing conditions. Application of dusting sulfur to logs that contained pupating borers greatly reduced densities of mites on emerging adult beetles and increased beetle survivorship. Uniform application to all logs in a glasshouse effectively eradicated the mite infestation. A bioassay showed that sulfur may physically impede the dispersal of immature mites by adhering to the cuticle, but sulfur vapor did not act as a toxin.

Animals↗

Persistent efficacy of doramectin and ivermectin against experimental infestations of Sarcoptes scabiei var. suis in swine.

Two studies were performed to compare the persistent efficacy of doramectin and ivermectin in swine experimentally infested with Sarcoptes scabiei var. suis. In the Study 1, 84 pigs were treated with doramectin, ivermectin, or saline solution on Day 0. Pigs were then challenged with mites on Days 0, 7, 14, 21, 28, 35, or 42. Weekly evaluations were performed for 5 weeks following challenge with mites. Weekly evaluations included physical examination for clinical signs of sarcoptic mange and collection of skin scrapings for determination of mite counts. In the Study 2, 80 pigs were treated with doramectin, ivermectin, or saline solution on Day 0, and challenged with mites on Days 3, 6, 9, 12, 15, 18, 21, 24, or 27. Weekly evaluations were performed for 6 weeks after challenge exposure. All negative-control (saline-treated) animals in both studies developed evidence of mite infestation. In the Study 1, doramectin prevented mite infestations 7 days longer than ivermectin. Results from the Study 2 indicated that the persistent efficacy of doramectin was 18 days on the basis of mite recovery. This was twice as long as the persistent efficacy of ivermectin, which was 9 days on the basis of mite recovery.

Animals↗

Efficacy of strips coated with Metarhizium anisopliae for control of Varroa destructor (Acari: Varroidae) in honey bee colonies in Texas and Florida.

Strips coated with conidia of Metarhizium anisopliae (Metschinkoff; Deuteromycetes: Hyphomycetes) to control the parasitic mite, Varroa destructor (Anderson and Trueman) in colonies of honey bees, Apis mellifera (Hymenoptera: Apidae) were compared against the miticide, tau-fluvalinate (Apistan) in field trials in Texas and Florida (USA). Apistan and the fungal treatments resulted in successful control of mite populations in both locations. At the end of the 42-day period of the experiment in Texas, the number of mites per bee was reduced by 69-fold in bee hives treated with Apistan and 25-fold in hives treated with the fungus; however mite infestations increased by 1.3-fold in the control bee hives. Similarly, the number of mites in sealed brood was 13-fold and 3.6-fold higher in the control bee hives than in those treated with Apistan and with the fungus, respectively. Like the miticide Apistan, the fungal treatments provided a significant reduction of mite populations at the end of the experimental period. The data from the broodless colonies treated with the fungus indicated that optimum mite control could be achieved when no brood is being produced, or when brood production is low, such as in the early spring or late fall. In established colonies in Florida, honey bee colony development did not increase under either Apistan or fungal treatments at the end of the experimental period, suggesting that other factors (queen health, food source, food availability) play some major role in the growth of bee colonies. Overall, microbial control of Varroa mites with fungal pathogens could be a useful component of an integrated pest management program for the honey bee industry.

Animals↗