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At least 145 records · Page 8Linked to original sources
PubMed2004

LC determination of citral in Cymbopogon citratus volatile oil.

It was the aim of this study to develop and validate a HPLC method for the quantitative determination of citral in Cymbopogon citratus volatile oil. The HPLC assay was performed using a Spherisorb CN column (250 mm x 4.6 mm, 5 microm), a n-hexane:ethanol (85:15) mobile phase and an UV detector (set at 233 nm). The following parameters were evaluated: linearity, precision, accuracy, specificity, quantification and detection limits. The method showed linearity in the range of 10.0-30.0 microg ml(-1). Precision and accuracy were determined at the concentration of 20 microg ml(-1). The concentration of citral in C. citratus volatile oil obtained with this assay was 75%. The HPLC method developed in this study showed an excellent performance (linearity, precision, accuracy and specificity) and can be applied to assay citral in volatile oil.

Acyclic Monoterpenes↗
PubMed2002

[Constituents analysis on volatile oil of Pogostemon cablin from different collection time cultivated in Hainan].

The constituents of the volatile oil of Pogostemon cablin collected from Wanning city, Hainan province and the influence owing to different collection time were undertaken by GC/MS combination technology. The results showed that main compounds was patchouli alcohol in stem oil and leaf oil, its contents were respectively 36.06% and 37.74%; pogostone was poor, its contents in stem oil and leaf oil were respectively 17.08% and 0.85%; the contents of ten compounds were over 1%, which was respectively beta-patchoulene, beta-elemene, transcaryophyllene, delta-guaiene, seychellene, alpha-patchoulene, aciphyllene, alpha-guaiene, patchouli alcohol and pogostone; the contents of volatile oil from June to August were respectively 0.8%, 0.7% and 0.6%; patchouli alcohol, higher in July and June(42.62% and 40.84%), lower in August(31.40%).

Azulenes↗
PubMed2004

Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2.

Melissa officinalis L. (Lamiaceae) has been used in a variety of practical applications in medical science. Our objective in the current study was to determine the effects of the volatile oil components of M. officinalis on Herpes simplex virus type 2 (HSV-2) replication in HEp-2 cells. Four different concentrations (25, 50, 100, 150 and 200 microg/ml) of volatile oils were examined. Experiments were carried out using HEp-2 cells. M. officinalis volatile oil was found to be non-toxic to HEp-2 cells up to a concentration of 100 micro/ml. It was, however, found to be slightly toxic at a concentration over of 100 microg/ml. The antiviral activity of non-toxic concentrations against HSV-2 was tested. The replication of HSV-2 was inhibited, indicating that the M. officinalis L. extract contains an anti-HSV-2 substance.

Animals↗
PubMed1999

Encapsulation of lemon oil by paste method using beta-cyclodextrin: encapsulation efficiency and profile of oil volatiles.

Microencapsulation of lemon oil was undertaken by kneading with beta-cyclodextrin, at a beta-cyclodextrin to lemon oil ratio of 88:12 (w/w). The resulting paste samples of the complex were vacuum- or spray-dried. Ten selected lemon oil flavor volatiles (alpha-pinene, sabinene, beta-pinene, beta-myrcene, limonene, gamma-terpinene, terpinolene, linalool, neral, and geranial) in the complex were analyzed periodically after 1, 2, 5, 10, 15, 20, and 30 min of kneading time. The results indicated that the levels of these volatiles were not significantly different (P > 0.05) irrespective of mixing time or type of the drying (vacuum- or spray-drying) used. An optimum mixing time was found to be 15 min, at which time the maximum encapsulation of lemon oil (97.7 mg/g of beta-cyclodextrin) was obtained in the complex powder.

Chromatography, Gas↗
PubMed2004

[Re-study on characteristic fingerprint of volatile oil from Herba Pogostemonis by GC].

OBJECTIVE: To establish the characteristic fingerprint and parameter of volatile oil from Herba Pogostemonis. METHODS: The dffferent collecting time and county samples of Herba Pogostemonis were determined by GC. RESULTS: Accuracy, stability and repeatability of the method were good. The same region samples had good stability and main constituents were same in different collection time, but content were different. The samples collected from different region were more different in constituents and content. CONCLUSION: The 11 components in common buildup the characterisitic fingerprint of volatile oil from Herba Pogostemonis. The ratiio of pogostone to patchoulic alcohol were used index parameter for quality evaluation and characteristics of producing region.

China↗
PubMed1986

Anticonvulsant activity of the volatile oil from the fruit of Tetrapleura tetraptera.

The anticonvulsant effect of the volatile oil extracted from the fresh fruits of Tetrapleura tetraptera was investigated in mice. The results obtained show that the fresh oil given intraperitoneally offers some protection against leptazol-induced convulsions. A dose of 0.4 ml of the oil per mouse protected 78% of the animals when administered 30 min prior to leptazol. While 0.6 ml offered no protection from death, the onset of convulsions and the average time of death was prolonged.

Africa, Western↗
PubMed1998

The effect of the volatile oils of Chenopodium ambrosioides and Thymus vulgaris against the larvae of Lucilia sericata (Meigen).

Nowadays, there are many problems with the use of chemical insecticides as resistance, environmental pollution, toxicity to man and animal... etc. All these required the investigation of a new line for controlling arthropod pests of medical and agricultural importance. The volatile oils of Chenopodium ambrosioides (American wormseed) and Thymus vulgaris (Tyme) proved to be effective against the third stage larvae of Lucilia sericata. The LC50 confidence limits were 70 ppm and 130 ppm for both volatile oils respectively. No doubt, the use of these plant volatile oils, which are widely distributed in the Egyptian flora, more cheap, more safe and without any pollution or hazard, is recommended.

Animals↗
PubMed2000

[The influence of external stimulation on content and quality of volatile oil in Lignun Santali albi].

The authors analyzed the quality of Ligmum Santali Albi formed by the external stimulation of hormone and windburn by GC-MS-DS. The results showed that the content of volatile oil is 2.34% in the heart wood formed in 10 years tree age of Santalum album (SA) after 2 years stimulation continuously with a definite concentration of hormone, which is near to the 25 years tree age of SA in the same place. The GC-MS analysis showed that the content of santalol and other chemical components in volatile oil are similar to the 25 years tree age of SA. It is indicated that a definite concentration of hormone stimulated the SA may shorten the formation of the heart wood. The heart wood can be also formed by the broken branches after 2 years windburn, but its content of volatile oil is only 1/2 of the heart wood formed by hormone stimulation.

Age Factors↗
PubMed2003

Isolation of volatile oil from thyme (Thymbra spicata) by steam distillation.

The effects of steam flow rate (1.03 and 0.64 L/h), distillation time, and particle size (0.50, 1.00, 2.05 mm) of Thymbra spicata on essential oil yield and sequence of extraction of compounds were studied. A logarithmic model fitted well to experimental data. The composition of the essential oil obtained by steam distillation was investigated for whole leaves only since the grinding had an adverse effect on yield. The oil obtained was very rich with respect to its carvacrol content. For both steam flow rates, oxygenated monoterpenes were recovered the most rapidly. Using whole leaves (2.05 mm) and a higher steam flow rate (1.03 L/h) for 75 min of distillation was chosen as the optimum which gives the lowest amount of monoterpene hydrocarbons, the complete recovery of oxygenated compounds, and the highest yield in a shorter time. Yield was 1.57% at this optimum condition. The oil was composed of 53.1% oxygenated compounds, 25.7% monoterpene hydrocarbons, 4.4% sesquiterpenes, and 14.1% p-cymene.

Chromatography, Gas↗