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At least 145 records · Page 8Linked to original sources
PubMed2005

Fatty acid transduction of nitric oxide signaling. Nitrolinoleic acid is a hydrophobically stabilized nitric oxide donor.

The aqueous decay and concomitant release of nitric oxide (*NO) by nitrolinoleic acid (10-nitro-9,12-octadecadienoic acid and 12-nitro-9,12-octadecadienoic acid; LNO2) are reported. Mass spectrometric analysis of reaction products supports a modified Nef reaction as the mechanism accounting for the generation of *NO by the aqueous reactions of fatty acid nitroalkene derivatives. Nitrolinoleic acid is stabilized by an aprotic milieu, with LNO2 decay and *NO release strongly inhibited by phosphatidylcholine/cholesterol liposome membranes and detergents when present at levels above their critical micellar concentrations. The release of *NO from LNO2 was induced by UV photolysis and triiodide-based ozone chemiluminescence reactions currently used to quantify putative protein nitrosothiol and N-nitrosamine derivatives. This reactivity of LNO2 complicates the qualitative and quantitative analysis of biological oxides of nitrogen when applying UV photolysis and triiodide-based analytical systems to biological preparations typically abundant in nitrated fatty acids. The results reveal that nitroalkene derivatives of linoleic acid are pluripotent signaling mediators that act not only via receptor-dependent mechanisms, but also by transducing the signaling actions of *NO via pathways subject to regulation by the relative distribution of LNO2 to hydrophobic versus aqueous microenvironments.

Animals↗
PubMed1994

Differential inhibition of platelet aggregation and calcium mobilization by nitroglycerin and stabilized nitric oxide.

We compared the mechanisms of the antiplatelet effects of nitroglycerin (NTG) and stabilized nitric oxide (NO). Stabilized NO was in the form of S-nitrosothiols [S-nitroso-albumin (S-NO-Alb) and S-nitrosocaptopril (S-NO-Cap)] or heme-NO [sodium nitro-prusside (SNP)]. The molecular structure of S-NO-Cap was confirmed by mass spectrometry. NTG, SNP, S-NO-Alb, and S-NO-Cap inhibited ADP-induced platelet aggregation dose dependently. The inhibitory IC50 value was 109 microM for NTG, 0.98 microM for SNP, 2.99 microM for S-NO-Alb, and 2.5 microM for S-NO-Cap. NTG (200 microM) released 15.4 microM nitrite anion into platelet-rich plasma (PRP) after 60-min incubation, to which platelets contributed 5.4 microM. On the other hand, SNP and S-NO-Cap released undetectable amounts of NO2- when incubated in either PRP or platelet-poor plasma (PPP). The platelet cytosolic calcium ion (Ca2+) concentration was measured fluorometrically in Fura-2-loaded gel-filtered platelets. Thrombin-induced Ca2+ mobilization was significantly inhibited by 10 microM NTG, SNP, S-NO-Alb, and S-NO-Cap, whereas resting Ca2+ was unaltered. Ca2+ mobilization was inhibited 28.6% by NTG, 91.9% by SNP, 90.0% by S-NO-Alb, and 92.7% by S-NO-Cap. These results demonstrate that NTG is an exogenous donor of NO, but releases it only slowly. On the other hand, SNP and S-nitrosothiols inhibited platelet aggregation by the action of stabilized NO incorporated in their structure and did not release NO. NTG and stabilized NO shared a common mechanism of antiplatelet activity, which involved inhibition of calcium mobilization.

Adult↗
PubMed2005

Migration stability of alpha-tocopherol in irradiated UHMWPE.

The oxidation resistance of irradiated ultra-high molecular weight polyethylene (UHMWPE) components used in total joint arthroplasty can be improved by adding alpha-tocopherol (vitamin E) through diffusion. To ensure long-term oxidative stability, a minimum alpha-tocopherol concentration needs to be maintained throughout these components. Migration of alpha-tocopherol out of the components is one mechanism that could compromise long-term oxidative stability. We hypothesized that alpha-tocopherol could elute out during standard implant fabrication steps such as cleaning as well as during in vivo use. We doped 85 kGy irradiated UHMWPE with alpha-tocopherol at 120 degrees C and homogenized at 120 degrees C. We determined the extent of elution of alpha-tocopherol or its effect on oxidative stability following cleaning in isopropyl alcohol (IPA) and following 5 million cycles (MC) of simulated normal gait in bovine serum. There was no significant elution of alpha-tocopherol in repeated and prolonged cleaning in IPA as measured by average surface and bulk alpha-tocopherol concentrations. There was no change in the oxidative stability following 5 MC of hip simulator testing, indicating minimal elution during simulated normal gait.

Animals↗
PubMed2004

Generating oxidation-resistant variants of Bacillus kaustophilus leucine aminopeptidase by substitution of the critical methionine residues with leucine.

Bacillus kaustophilus leucine aminopeptidase (bkLAP) was sensitive to oxidative damage by hydrogen peroxide. To improve its oxidative stability, the oxidation-sensitive methionine residues in the enzyme were replaced with leucine by site-directed mutagenesis. The variants, each with an apparent molecular mass of approximately 54 kDa, were overexpressed in recombinant Escherichia coli M15 cells and purified to homogeneity by nickel-chelate chromatography. The specific activity for M282L, M285L, M289L and M321L decreased by more than 43%, while M400L, M426L, M445L, and M485L showed 191, 79, 313, and 103%, respectively, higher activity than the wild-type enzyme. Although the mutations did not cause significant changes in the K(m) value, more than 67.8% increase in the value of k(cat)/K(m) was observed in the M400L, M426L, M445L and M485L. In the presence of 50 mM H2O2, most variants were more stable with respect to the wild-type enzyme, indicating that the oxidative stability of the enzyme can be improved by engineering the methionine residues.

Amino Acid Substitution↗
PubMed1999

Directed evolution of a fungal peroxidase.

The Coprinus cinereus (CiP) heme peroxidase was subjected to multiple rounds of directed evolution in an effort to produce a mutant suitable for use as a dye-transfer inhibitor in laundry detergent. The wild-type peroxidase is rapidly inactivated under laundry conditions due to the high pH (10.5), high temperature (50 degrees C), and high peroxide concentration (5-10 mM). Peroxidase mutants were initially generated using two parallel approaches: site-directed mutagenesis based on structure-function considerations, and error-prone PCR to create random mutations. Mutants were expressed in Saccharomyces cerevisiae and screened for improved stability by measuring residual activity after incubation under conditions mimicking those in a washing machine. Manually combining mutations from the site-directed and random approaches led to a mutant with 110 times the thermal stability and 2.8 times the oxidative stability of wild-type CiP. In the final two rounds, mutants were randomly recombined by using the efficient yeast homologous recombination system to shuffle point mutations among a large number of parents. This in vivo shuffling led to the most dramatic improvements in oxidative stability, yielding a mutant with 174 times the thermal stability and 100 times the oxidative stability of wild-type CiP.

Coprinus↗
PubMed2000

Effect of dry honey on oxidation in turkey breast meat.

The effect of adding dry honey to turkey breast meat on oxidative stability was measured using TBA, volatile headspace, and oxidative stability index. Ground turkey breast meat was mixed with different concentrations of dry honey (0, 1, 5, 10, 15, and 20%) then cooked in polyethylene bags to 72 C. Oxidative stability measurements were taken for raw meat and cooked meat and for cooked meat after 48 h of storage at 4 C. The proximate composition of the raw meat was 73.9% moisture, 23.2% protein, and 1.3% fat. Gas headspace analysis determined hexanal to be the most abundant volatile compound. Hexanal content decreased as the amount of added honey increased in both freshly cooked meat and in meat stored for 48 h at 4 C. The TBA values also decreased with increasing levels of added honey in the freshly cooked and 48-h-stored meats. The percentage inhibition of oxidation for the 5, 10, 15, and 20% samples increased from 50 to 76% for the freshly cooked meat and from 34 to 88% for the 48-h-stored meat. The oxidative stability index increased with increasing concentrations of honey from 0.28 h (control) to 7.73 h (20% honey). Addition of up to 15% honey inhibited the development of oxidative compounds in cooked turkey meat, with little further inhibition observed compared to 20% honey.

Aldehydes↗
PubMed2004

Preparation of soybean oil-based greases: effect of composition and structure on physical properties.

Vegetable oils have significant potential as a base fluid and a substitute for mineral oil in grease formulation. Preparation of soybean oil-based lithium greases using a variety of fatty acids in the soap structure is discussed in this paper. Soy greases with lithium-fatty acid soap having C12-C18 chain lengths and different metal to fatty acid ratios were synthesized. Grease hardness was determined using a standard test method, and their oxidative stabilities were measured using pressurized differential scanning calorimetry. Results indicate that lithium soap composition, fatty acid types, and base oil content significantly affect grease hardness and oxidative stability. Lithium soaps prepared with short-chain fatty acids resulted in softer grease. Oxidative stability and other performance properties will deteriorate if oil is released from the grease matrix due to overloading of soap with base oil. Performance characteristics are largely dependent on the hardness and oxidative stability of grease used as industrial and automotive lubricant. Therefore, this paper discusses the preparation methods, optimization of soap components, and antioxidant additive for making soy-based grease.

Chemical Phenomena↗
PubMed2006

Facile synthesis of tin oxide nanoparticles stabilized by dendritic polymers.

Tin(IV) oxide nanoparticles were synthesized via the reaction of carbon dioxide with stannate ions immobilized by dendritic polymers. For PAMAM and PPI dendrimer hosts, resultant nanoparticle diameters were 2.5-3 nm; 3-3.5 nm nanoparticles resulted from use of a poly(ethyleneimine) hyperbranched polymer. Our conditions represent the only precedent for SnO2 nanoparticulate growth using dendritic architectures, as well as a novel application for CO2 as a reactive gas for the controlled growth of metal oxide nanoparticles.

Journal Article↗
PubMed2006

Biobased grease with improved oxidation performance for industrial application.

Vegetable oils have significant potential as a base fluid and a substitute for mineral oil for grease formulation. This paper describes the preparation of biobased grease with high oxidative stability and a composition useful for industrial, agriculture/farming equipment, and forestry applications. The process utilizes more oxidatively stable epoxy vegetable oils as the base fluid, metal-soap thickener, and several specialty chemicals identified to address specific applications. Performance characteristics of greases used for industrial and automotive applications are largely dependent on the hardness and the oxidative stability of grease. Grease hardness was determined using standard test methods, and their oxidative stabilities were determined using pressurized differential scanning calorimetry and rotary bomb oxidation tests. Wear data were generated using standard test methods in a four-ball test geometry. Results indicate that grease developed with this method can deliver at par or better performance properties (effective lubrication, wear protection, corrosion resistance, friction reduction, heat removal, etc.) than existing mineral oil-based greases currently used in similar trades. Therefore, developed greases can be a good substitute for mineral oil-based greases in industrial, agriculture, forestry, and marine applications.

Chemical Phenomena↗
PubMed2001

Impact of tween 20 hydroperoxides and iron on the oxidation of methyl linoleate and salmon oil dispersions.

To determine the role of surfactant hydroperoxides on the oxidative stability of fatty acids, the oxidation of methyl linoleate micelles and salmon oil-in-water emulsions was measured as a function of varying Tween 20 hydroperoxide concentrations. Increasing Tween 20 hydroperoxide concentrations from 3.5 to 14.7 micromol hydroperoxide/g Tween 20 decreased the lag phase of headspace hexanal formation but did not increase the total amount of hexanal formed in methyl linoleate/Tween 20 micelles. In the micelle system, Fe(2+) decreased the lag phase of hexanal formation but increased total hexanal concentrations only in micelles with the highest Tween 20 hydroperoxide concentrations (14.7 micromol hydroperoxide/g surfactant). Increasing Tween 20 surfactant hydroperoxide concentrations also increased the oxidation of salmon oil-in-water emulsions as determined by lipid hydroperoxides and headspace propanal. In both the micelle and emulsion systems, the prooxidant effect of Fe(2+) decreased with increasing Tween 20 hydroperoxide concentrations. These data show that surfactant hydroperoxides such as those in Tween 20 could decrease the oxidative stability of lipids in food emulsions.

Drug Interactions↗
PubMed1994

Endogenous skeletal muscle antioxidants.

Skeletal muscle is susceptible to oxidative deterioration due to a combination of lipid oxidation catalysts and membrane lipid systems that are high in unsaturated fatty acids. To prevent or delay oxidation reactions, several endogenous antioxidant systems are found in muscle tissue. These include alpha-tocopherol, histidine-containing dipeptides, and antioxidant enzymes such as glutathione peroxidase, superoxide dismutase, and catalase. The contribution of alpha-tocopherol to the oxidative stability of skeletal muscle is largely influenced by diet. Dietary supplementation of tocopherol has been shown to increase muscle alpha-tocopherol concentrations and inhibit both lipid oxidation and color deterioration. Dietary selenium supplementation has also been shown to increase the oxidative stability of muscle presumably by increasing the activity of glutathione peroxidase. The oxidative stability of skeletal muscle is also influenced by the histidine-containing dipeptides, carnosine and anserine. Whereas carnosine and anserine are affected by diet less than alpha-tocopherol and glutathione peroxidase, their concentrations vary widely with species and muscle type. In pigs, beef, and turkey muscle, carnosine concentrations are greater than anserine, while the opposite is true in rabbit, salmon, and chicken muscle. Anserine and carnosine are found in greater concentrations in muscle high in white fibers, with chicken white muscle containing over fivefold more anserine and carnosine than red muscle. Anserine and carnosine are thought to inhibit lipid oxidation by a combination of free radical scavenging and metal chelation.

Animals↗
PubMed2005

Electrostatically stabilized metal oxide particle dispersions in carbon dioxide.

We report electrostatic stabilization of micrometer-sized TiO(2) particles at long range (several micrometers) in liquid and supercritical CO(2) despite the ultralow dielectric constant, as low as 1.5. The counterions were solubilized in dry reverse micelles, formed with a low-molecular weight cationic perfluoropolyether trimethylammonium acetate surfactant, to prevent ion pairing with the particle surface. Dynamic light scattering and settling velocities indicate a particle diameter of 620-740 nm. The electrophoretic mobility of -2.3 x 10(-8) m(2)/V s indicated a particle charge on the order of -1.7 x 10(-17) C, or 105 elementary negative charges per particle. The balance of particle compression by an electric field versus electrostatic repulsion generated an amorphous arrangement of particles with 5-9 mum spacing, indicating Debye lengths greater than 1 mum. Scattering patterns also indicate that chains of particles may be achieved in CO(2) by dielectrophoresis with alternating fields. The electrostatic stabilization has been achieved by solubilizing a small concentration of counterions in only a small fraction of the reverse micelles in the double layer. Whereas many low-molecular weight surfactants have been shown to form reverse micelles in CO(2), very few polymers are able to stabilize micrometer-sized colloids sterically. Thus, electrostatic stabilization has the potential to expand markedly the domain of colloid science in apolar supercritical fluids.

Journal Article↗
PubMedDate not supplied

Hemp-seed and olive oils: their stability against oxidation and use in O/W emulsions.

Hemp-seed oil has several positive effects on the skin: thanks to its unsaturated fatty acid (PUFA) content it alleviates skin problems such as dryness and those related to the aging process. We present a comparative study of hemp-seed and olive oils, determining some physicochemical indices and evaluating their stability against oxidation. The peroxide value of hemp-seed oil was below 20, the threshold limit for edible oils. Hemp-seed oil was less stable against peroxidation than olive oil, but MDA and MONO assays showed its stability to be above expectations. The chlorophyll contained in extra virgin olive oil had a higher photostability than that contained in hemp-seed oil, possibly due to the larger amount of antioxidant in the olive oil. A certain amount of Vitamin E was found in hemp-seed oil. Since quality analyses indicated that hemp-seed oil is relatively stable, emulsions were prepared with the two oils, and their stability and rheological characteristics were tested. Some of the resulting gel-emulsions were suitable for spraying on the skin.

Cannabis↗
PubMedDate not supplied

[Physico-chemical characteristics of different types of vegetable fats and oils used in the manufacture of candies].

The quality of three vegetable fats (cocoa butter and two commercial fats) and three roasted nut oils (almond, hazelnut and peanut) used as raw material in the chocolate products manufacturing was studied. The hydroperoxide content, oxidative stability and fatty acid composition were determined and its health repercussion by atherogenicity and thrombogenicity indexes. Two commercial fats and cocoa butter showed higher oxidative stability, atherogenic and thrombogenic properties than oils because of its different fatty acid profiles. Peroxide value was a low reliability parameter of raw material shelf live. Rancimat presented a good correlation with the unsaturation index of different fats and oils, it was a better index than peroxide value. In the chocolate products manufacturing it would be advisable a good raw material selection and formulation in order to get a balance between technological properties, organoleptic qualities and the influence on the health. Those raw material with less primary oxidation and higher oxidative stability were also those of higher atherogenicity and thrombogenicity indexes.

Analysis of Variance↗
PubMed2000

Effect of dietary alpha-tocopheryl acetate and ascorbic acid on rabbit semen stored at 5 degrees C.

The objective of this research was to verify the effects of dietary alpha-tocopheryl acetate (50 vs. 200 mg/kg diet) and ascorbic acid (0 vs. 1 g/L water) on the relative amounts on semen and motion characteristics, oxidative stability and fertilizing ability of rabbit spermatozoa stored for 24 h at 5 degrees C. A high amount of dietary (alpha-tocopheryl acetate significantly increased the level of Vitamin E in the semen (0.90 vs. 0.41 micromol/L) and its oxidative stability after storage (Thiobarbituric Acid Reactive Substances-- TBARS 15.88 vs. 20.90 nmol Malondialdehyde--MDA/mL). Ascorbic acid showed a different effect in relation to the Vitamin E status of animals: when associated with the higher level of Vitamin E it increased the (alpha-tocopherol and the oxidative stability of semen (2.67 micromol/L and 12.25 nmol MDA/mL, respectively), whereas both parameters were reduced with lower Vitamin E (0.13 micromol/L and 21.20 nmol MDA/mL). Semen traits were not modified by the separate supplementation of supranutritional levels of vitamins, whereas their combination significantly improved the viability and the kinetics of spermatozoa (e.g. track speed: 95.13 vs. 71.31 microm sec(-1)) with an increase in fertility rate (70.0 vs. 63.3; P=0.06) that could be considered almost significant.

Animals↗
PubMed2001

[Correlation between severity of angina, its stability, and oxidative modification of lipids in patients with ischemic heart disease].

AIM: To elucidate the relations between angina severity, stability, concomitant arterial hypertension, diabetes mellitus, plasma oxidation. MATERIAL AND METHODS: The study included 152 patients with stable angina of effort (functional class II-III). Of them, 67 had concomitant arterial hypertension (AH), 20 AH and compensated diabetes mellitus type II, 14 patients with anginal functional class III were admitted to hospital for unstable angina, 11 patients with IHD with unstable angina and 28 healthy donors. The study was made of dynamic features of lipid oxidation using copper-induced plasma oxidation. Lipids were measured with enzyme assays. RESULTS: The degree of LP oxidation increases with aggravation of angina. IHD risk factors activate atherogenic modification of lipids in patients with stable angina. There was no differences between the groups in content of low density lipoproteins cholesterol. CONCLUSION: Plasma resistance to oxidation may serve an additional criterion for assessment of severity and stability of various IHD forms.

Angina Pectoris↗
PubMed2003

Dietary supplementation with a natural carotenoid mixture decreases oxidative stress.

OBJECTIVE: To determine whether dietary supplementation with a natural carotenoid mixture counteracts the enhancement of oxidative stress induced by consumption of fish oil. DESIGN: A randomised double-blind crossover dietary intervention. SETTING: Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, The University of Reading, Whiteknights PO Box 226, Reading RG6 6AP, UK. SUBJECTS AND INTERVENTION: A total of 32 free-living healthy nonsmoking volunteers were recruited by posters and e-mails in The University of Reading. One volunteer withdrew during the study. The volunteers consumed a daily supplement comprising capsules containing fish oil (4 x 1 g) or fish oil (4 x 1 g) containing a natural carotenoid mixture (4 x 7.6 mg) for 3 weeks in a randomised crossover design separated by a 12 week washout phase. The carotenoid mixture provided a daily intake of beta-carotene (6.0 mg), alpha-carotene (1.4 mg), lycopene (4.5 mg), bixin (11.7 mg), lutein (4.4 mg) and paprika carotenoids (2.2 mg). Blood and urine samples were collected on days 0 and 21 of each dietary period. RESULTS: The carotenoid mixture reduced the fall in ex vivo oxidative stability of low-density lipoprotein (LDL) induced by the fish oil (P=0.045) and it reduced the extent of DNA damage assessed by the concentration of 8-hydroxy-2'-deoxyguanosine in urine (P=0.005). There was no effect on the oxidative stability of plasma ex vivo assessed by the oxygen radical absorbance capacity test. beta-Carotene, alpha-carotene, lycopene and lutein were increased in the plasma of subjects consuming the carotenoid mixture. Plasma triglyceride levels were reduced significantly more than the reduction for the fish oil control (P=0.035), but total cholesterol, HDL and LDL levels were not significantly changed by the consumption of the carotenoid mixture. CONCLUSIONS: Consumption of the natural carotenoid mixture lowered the increase in oxidative stress induced by the fish oil as assessed by ex vivo oxidative stability of LDL and DNA degradation product in urine. The carotenoid mixture also enhanced the plasma triglyceride-lowering effect of the fish oil. SPONSORSHIP: : The study was supported by funding from the Greek Studentship Foundation and from Unilever Bestfoods plc. Carotenoids were contributed by Overseal Foods plc.

Adult↗