PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “STAPHYLOCOCCAL PHAGES”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 145 records · Page 8Linked to original sources
PubMed1975

[Clinical study of the efficacy of staphylococcal anatoxin in relation to the phage group to which the staphylococcal strains--the causative agents of infection--belong].

In comparing the bacteriophage group reference of the strains of pathogenic staphylococci isolated in case of postoperative complications from children given staphylococcus toxoid for prophylactic purpose and from control group it was found that prophylactic vaccinations of staphylococcus toxoid created the most intense immunity against staphylococci of the I bacteriophage group. There was found no significant association between the efficacy of the therapy and bacteriophage reference of staphylococci--the causative agents of the infection.

Bacteriophage Typing↗
PubMed1995

Random mutagenesis of staphylococcal nuclease and phage display selection.

Multiple cycles of mutagenesis and phage display selection have been investigated as a method for obtaining enzymes with altered catalytic properties. A library of staphylococcal nuclease mutants displayed on phage was created by error-prone PCR mutagenesis and selected for binding to thymidine- or guanosine-containing substrate analogs. After discarding non-binders, the binding mutants were then subjected to further mutagenesis and selection rounds. After four mutagenesis and selection cycles, the catalytic properties of some of the resulting nucleases were studied and one nuclease with nine accumulated mutations was found to have a two-fold reduction in kcat for DNA hydrolysis, but a two-fold increase in kcat/Km for hydrolysis of a thymidine containing small molecule substrate. The possibility of this technique for in vitro evolution of enzyme properties is discussed.

Amino Acid Sequence↗
PubMed2001

Phage conversion of Panton-Valentine leukocidin in Staphylococcus aureus: molecular analysis of a PVL-converting phage, phiSLT.

Staphylococcal Panton-Valentine leukocidin (PVL) is an important virulence factor, which causes leukocytolysis and tissue necrosis. Our previous report on the existence of the PVL genes (lukS-PV and lukF-PV) on the genome of prophage phiPVL in the Staphylococcus aureus strain V8 suggested the horizontal transmission of PVL genes by temperate bacteriophage among S. aureus (Kaneko, et al., 1998. Gene 215, 57-67). Here, we demonstrated the phage conversion of S. aureus leading to the production of PVL by discovery of a novel PVL-carrying phage, phiSLT (Staphylococcal Leukocytolytic Toxin) from a clinical isolate of S. aureus. phiSLT was able to lysogenize several clinical isolates of PVL-negative S. aureus strains as well as strain RN4220 at the conserved 29-bp sequence (attB) and all the lysogenized S. aureus strains had the ability to produce PVL. phiSLT had an elongated head of about 100x50 nm and a flexible tail of 400 nm long, that was quite different from phiPVL which had an isometric hexagonal head of about 60 nm diameter. The linear double-stranded phiSLT genome comprised 42,942 bp with 29-bp attachment core sequences and contained 62 open reading frames. Only 6.4 kbp region containing lysis cassette, PVL genes, attP, integrase, and orf204 of phiSLT was identical to that of phiPVL, while other regions were different from those of phiPVL. Thus, it can be concluded that PVL genes are carried by different temperate phages, which have the same attachment site.

Amino Acid Sequence↗
PubMed1985

Staphylococcal enterotoxin A is encoded by phage.

The gene for staphylococcal enterotoxin A (entA), in two wild-type strains, is carried by related temperate bacteriophages. Hybridization analysis of DNA from entA-converting phage PS42-D and its bacterial host suggests that this phage integrates into the bacterial chromosome by circularization and reciprocal crossover (the Campbell model) and that the entA gene is located near the phage attachment site. DNA from three of eight staphylococcal strains that did not produce enterotoxin A and seven wild-type enterotoxin A-producing (EntA+) strains had extensive homology to the entA-converting phage PS42-D DNA, although there was a high degree of restriction-fragment length polymorphisms. At least one EntA+ strain did not produce detectable viable phage after induction. These data indicate that a polymorphic family of Staphylococcus aureus phages (some of which may be defective) can carry the entA gene.

Enterotoxins↗
PubMedDate not supplied

[Dependence of the effectiveness of phage infection on energy status of staphylococcal cells].

Studies of phage infection efficiency in Staphylococcus aureus showed its dependence on the energy processes. Addition of KCN, dycyclohexylcarbodiimide and chlorocarbonylcyanide-phenylhydrazone at the moment of cells contact with bacteriophages lowered phagolysis efficiency to 49.5-68.0%. These inhibitors influenced on cell specifically leading to suppressing generators of protonmotive force and to dissipating membrane potential. Adding phages to the suspension of bacterial led to the dissipation of membrane potential.

Carbonyl Cyanide m-Chlorophenyl Hydrazone↗