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Systemic distribution of 14C-labeled formaldehyde applied in the root canal following pulpectomy.

The systemic distribution of 14C-labeled formaldehyde which had been placed in the root canals of the canines of cats following pulpectomies was studied using liquid scintillation counting and wholebody autoradiographic technique. Radioactive 14C which had been placed in the canals was found in the plasma 30 min after the root canal procedure. The recovery of systemic 14C radioactivity increased with time. In addition, it seemed that approximately 3% of the dose placed in the teeth was excreted in the urine within 36 h. Whole-body autoradiograms indicated extensive concentration of 14C radioactivity in tissues other than those analyzed with the liquid scintillation technique.

Animals

Increased permeability of enamel to iodide ions following the ingestion of cookies varying in sucrose or fat content.

Experiments showed that the intraoral iodide permeability (Ip) method can be used in a reproducible and sensitive manner with solid foods. Ingestion of 5-gram portions of cookies made with defined concentrations of sucrose or fat led to an increased Ip (due to demineralization) of Streptococcus mutans-covered bovine enamel blocks in vivo. Demineralization increased with time to a maximum of 45 min, and the pH of the plaque dropped accordingly. Continued exposure in the mouth beyond 45 min led to an elevation of the pH and a decrease in delta Ip consistent with remineralization of the enamel. Control blocks worn without ingestion of cookies exhibited negative delta Ip values. Demineralization increased with increasing sucrose content of the cookies and reached a plateau when cookies containing 1.08 g sucrose per morsel were administered. Cookies prepared without added sucrose gave a high delta Ip. High fat content raised the delta Ip when sucrose was low. These findings are consistent with clinical and other observations, and emphasize the complex relation between foods and enamel demineralization.

Animals

Effect of phytate and hexadecylamine on the permeability of bovine dental enamel.

The effect of hexadecylamine on the permeability of bovine dental enamel was investigated using a radiochemical diffusion technique. Treatment with an aqueous solution of hexadecylamine reduced the permeability of the enamel by about 20 per cent. Pre-treatment of the enamel with an aqueous solution of phytate followed by hexadecylamine yielded a reduction in permeability of approximately 70 per cent. The observed effects were similar for ionic (86Rb+ and 36Cl-) and non-ionic [( 3H]-sorbitol and [14C]-glycerol) compounds. The strong permeability-reducing effect of the combined phytate-amine treatment may also be anticariogenic.

Amines

The effect of 25% tannic acid on prepared dentin: a scanning electron microscope-methylene blue dye study.

The effect on the permeability of prepared dentin treated with 25% tannic acid and 6% citric acid was compared with the untreated dentinal surface. Methylene blue was applied to the dentin surface after treatment to evaluate penetration into dentinal tubules. The tannic acid solution reduced or prevented dye penetration of the dentinal tubules. Citric acid treatment permitted severe penetration. The 25% tannic acid solution removed the smear layer while inhibiting penetration of the dye.

Acid Etching, Dental

Comparison of in vitro and in vivo dog dentin permeability.

A method is described which permits the quantitation of the rate of permeation of 131I through dog dentin, both in vivo and in vitro. The results demonstrate the rapidity of the systemic appearance of substances placed on intact dentin. Comparison of the rate of permeation of 131I made in vivo and in vitro, on the same teeth, indicates the rates are very similar.

Animals

Diffusion of fluorides in human dental enamel in vitro.

The diffusion of fluoride (F) from sodium fluoride, sodium fluorosilicate, and sodium monoflurophosphate solutions (containing 0.1 per cent F in physiological saline) was studied using a two-chamber diffusion cell separated by enamel membrane. The diffusion coefficient, D in cm2s-1, of F was determined under steady-state conditions over 3 weeks. The D value of F for NaF was significantly higher than for Na2SiF6 and Na2PO3F (p less than 0.001; unpaired t-test). F for acidic Na2SiF6 diffused more rapidly than for Na2PO3F (p less than or equal to 0.001), presumably as HF molecule. Despite the F in the tested solutions being in different forms, i.e. simple ion in NaF or mainly complex ions as in Na2SiF6 and Na2PO3F, the diffusivity of the F in enamel for these compounds was of the same order of magnitude (D = 10(-9)cm2s-1). The findings support the concept that enamel can behave as an ion-selective membrane with certain molecular-sieve effects.

Dental Enamel