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[Interference of the intestinal ecosystem of mice in the oral administration of lyophilized live bacteria].

Oral vaccines mode by living or killed bacteria are commonly used to restore normal intestinal flora; it's not well know, however, which bacteria play the leading part in this ecosystem. In the present paper Authors have intended to compare the effectiveness of an oral vaccine, made by Bifidobacterium bifidus and Lactobacillus acidofilus, with another of similar use, made by Enterococci, to increase the mouse resistance to lethal Salmonella enteritidis infections. According to collected microbiological data and M.E.S. photos, the association Bifidob./Lactobac. is more effective than Enterococci to enhance resistance to the experimental infection.

Administration, Oral↗

[Isolation and characteristics of plasmid DNA from Lactobacilli].

It was shown on a model of 2 collection strains of lactobacilli that their cultivation on a synthetic multicomponent medium with addition of threonine and subsequent exposure of the cells to lysozyme at 0 degrees C and to alkaline solution of sodium dodecylsulfate at 60 degrees C provided highly efficient detection of plasmid DNA in these organisms. Circular molecules of the plasmid DNA of the 7-kv length, their dimer forms and linear molecules were detected in both of the strains.

DNA, Bacterial↗

Adherence of specific yogurt micro-organisms to human peripheral blood lymphocytes.

Yogurt lactic-acid-bacteria (LAB) consisting of Lactobacillus bulgaricus and Streptococcus thermophilus were evaluated for their capacity to bind to human peripheral blood lymphocytes (PBL). These micro-organisms adhere to human T lymphocytes, and bind to B lymphocytes, in high and low frequencies, respectively. In addition, a quantitative analysis of LAB binding to PBL was carried out using the same parameters previously applied to a Salmonella model. The effect of yogurt LAB on the natural anti-bacterial activity exerted by PBL was examined. Lymphocyte pretreatment with bacteria did not affect such functions. These findings are discussed in the light of the well known ability of yogurt LAB to modulate the immune response.

B-Lymphocytes↗

[Isolation and identification of Gardnerella vaginalis].

Thirty-four strains of Gardnerella vaginalis were studied. They were isolated from non specific vaginitis. A presumptive identification can be based on colonial morphology, Gram stain characteristics, negative catalase and oxidase test. The differentiation of Gardnerella vaginalis from other negative catalase coccobacilli is based on the acid formation of carbohydrates, enzymatic test, analysis of short chain volatile and non volatile end products of fermentation in GLC. All Gardnerella strains produce acid from glucose, maltose, starch and are hippurate and alpha glucosidase positive. They are sulfonamide resistant, acetic, lactic and succinic acids are detected by gas liquid chromatography.

Actinomyces↗

[Simple method of determining total deoxyribonucleosides in human urine].

A method for quantitative estimation of total deoxyribonucleosides in human urine is based on the preliminary purification of urine usina ion exchange resin Dowex 1X 8 in OH- form, subsequent treatment of urine with acetone-ethynol mixture and the quantitative estimation of deoxyribonucleosides content by a microbiologic method. Urine of healthy persons contained 95.4+/-12.7 microng of deoxyribonucleosides.

Bacteriological Techniques↗

Effect of a lactobacilli preparation on traveler's diarrhea. A randomized, double blind clinical trial.

The effectiveness of prophylactic ingestion of a commercial preparation of lactobacilli (Lactinex) for the prevention or modification of traveler's diarrhea was tested in a randomized double blind clinical trial in 50 volunteer travelers to Mexico from the United States. Twenty-six subjects received the lactobacilli preparation and 24 received placebo. The incidence of diarrhea and its duration during the 4 weeks of observation were quite similar for the two preparations: 35% for lactobacilli-treated subjects and 29% for placebo subjects. Typically, the diarrhea was mild, lasting 2 days. From the observations during this study we conclude that prophylactic ingestion of lactobacilli for 1 week does not reduce the incidence or duration of traveler's diarrhea either during the period of ingestion or during the following 3 weeks.

Adolescent↗