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[Skin surface lipids and sweat of hand in girl students who complained of impaired skin on the hand by using household detergents].

A questionnaire was presented to female university students concerning the relationship between the use of household detergent and the occurrence of impaired hands. In addition, in order to determine whether there was any relationship between the amounts and qualities of skin surface film and the incidence of impairments of hands, the following experiment was undertaken. From among the 1,861 female university students, 28 girls between the ages of 21-22 were chosen; 14 of whom had complained of impaired skin of hands (impairment group) attributable to household detergent during the past one year and 14 who had not experienced such disorders, either of the hands or elsewhere (non-impairment group) during the same period. The method of collecting skin surface lipids and sweat simultaneously is for the subjects to wear special gloves made of a given cotton fabric by the modified method of Straus & Pochi for a fixed of time. Then, the skin surface lipids and sweat were analyzed to determine the differences between the impairment group and the non-impairment group. The following results were obtained: 1) Of the 1,861 people surveyed, 496 or 26.7% of them stated that they had suffered from impaired skin due to the use of household detergents during the past one year. The most frequent site was the hands, including wrists, and the main symptom was dry skin. There were some seasonal changes in the occurrence of the impaired skin, with the highest occurrence in winter. There was a significant difference between the occurrence in winter, and that in spring, summer and autumn (p less than 0.01). 2) During the hot season, (average temperature 23-29 degrees C), the arithmetic averages and their standard deviation of the skin surface lipids of both the impairment group (N = 14) and the non-impairment group (N = 14) were 29.10 +/- 7.89 micrograms/cm2/8 hr and 45.98 +/- 23.29 micrograms/cm2/8 hr, respectively. In the cold season, (average temperature 5-13 degrees C), these become 31.95 +/- 16.69 micrograms/cm2/8 hr, and 53.45 +/- 24.16 micrograms/cm2/ 8 hr, respectively. In both these seasons, it was found that the amount of skin surface lipids of the impairment group was significantly less (hot season p less than 0.05, cold season p less than 0.01) than that of the non-impairment group.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗

[Harlequin syndrome (unilateral flushing and sweating attack) due to a spinal invasion of the left apical lung cancer].

A 54-year-old man was admitted to our hospital because of a persistent pain of the left cervix and scapular region of three-month duration and an abnormal shadow in the chest roentgenograms. Neurological examinations, chest roentgenograms, chest CT scanning, vertebral tomograms and myelogram revealed Pancoast's syndrome concomitant with Horner's syndrome. Four months later, the patient complained of a sudden onset of unilateral flushing and sweating appearing on the right face, cervix and upper chest. Eye drop tests with cocaine, epinephrine and tyramine indicated the lesion of ciliospinal centers between the 8th cervical and 2nd thoracic spines. The unilateral flushing and sweating attack appearing on the intact side without Horner's syndrome seemed to be an excessive response by an intact sympathetic pathway, the other side failing to respond because of a sympathetic deficit.

Flushing↗

Cl- permeability of sweat duct cell membranes: intracellular microelectrode analysis.

Cl- permeability in the reabsorptive sweat duct (RSD) epithelium from normal subjects was studied using electrophysiological techniques. The average basolateral membrane potential (Vb) of normal ducts was -36.8 +/- 0.8 mV and the average apical membrane potential (Va) was -27.2 +/- 0.8 mV (n = 45). Amiloride in the lumen of microperfused sweat ducts hyperpolarized Va by 34.3 +/- 3.1 mV and Vb by 25.7 +/- 3.1 mV (n = 12) with a small but significant increase in voltage divider ratio (Ra/Rb) from 4.2 +/- 0.8 to 5.0 +/- 0.8 (n = 8). Cl- substitution in the lumen depolarized Va by +37 +/- 4.2 (n = 11) accompanied by a significantly larger increase in Ra/Rb from 4.8 +/- 2.6 (n = 8) to 7.0 +/- 3.1 (n = 8). Bath Cl- substitution depolarized Vb by +24.3 +/- 2.7 mV (n = 15) while decreasing Ra/Rb from 3.2 +/- 0.7 to 1.9 +/- 0.4 (n = 7). These results indicated a significant Cl- permeability in both apical and basolateral membranes. Removing Cl- from the lumen significantly decoupled Va and Vb and restricted the amiloride-induced hyperpolarization to the apical membrane. This result may suggest that intracellular Cl- might be responsible for coupling Va and Vb and that hyperpolarization of Va and Vb by amiloride may result in changes in intracellular Cl-. Alternatively, Va and Vb could be coupled through a Cl- sensitive paracellular shunt. These results are consistent with Cl- permeability in both apical and basolateral membranes of duct cells. However, the question of whether paracellular Cl- permeability is important in Cl- uptake cannot be determined from the present data.

Amiloride↗

Eccrine sweat gland reaction. A histological and immunocytochemical study.

Secondary alteration of eccrine sweat glands is frequent in neoplastic and inflammatory skin diseases. The histological changes are characterized by a remarkable variability. However, a systematic classification of these reactions does not exist. We examined routine histological sections of biopsy specimens and found morphological alterations of eccrine sweat gland in the secretory coil, the duct, and the surrounding connective tissue. Dilation with or without retention was the most common histological variation seen in the secretory segments and the ducts. In association with dilation, flattening of the secretory and ductal cells occurs. Other cell changes were hydropic alteration and vacuolization of the secretory cells; seldom was a thickening of the cuticle observed. A striking phenomenon was the proliferation of ductal epithelium in different skin tumors. We found histological changes of the surrounding connective tissue less frequently: marked periglandular fibrosis and mucinous degeneration occurred.

Biopsy↗

Impaired sweating in the diabetic neuropathic foot and its influence on skin flora.

Three groups each of 10 subjects, were defined by clinical and neurophysiological studies: diabetic with neuropathy (Group A), diabetic without neuropathy (Group B) and normal control (Group C). Sweating on the foot was quantified and the bacterial flora on the dorsum of the foot and deltoid area were examined. There were no significant differences in type of density of bacterial species found on either foot or deltoid regions between any of the three groups. Diabetics free of clinical infection have a similar skin microbial flora to non-diabetics even when sweating is seriously impaired. It is therefore unlikely that a change in the resident microbial flora is involved in the propensity of diabetic neuropathic patients to foot ulceration and infection.

Actinomycetales↗

Primary mucinous sweat gland carcinoma of the eyelid.

A 76-year-old woman had a slowly enlarging lesion of the right lower eyelid. After obtaining incisional biopsy results consistent with adenocarcinoma, we removed the tumor and made a final diagnosis of primary mucinous adenocarcinoma of the sweat gland. The defect was repaired using ipsilateral upper lid transpositional tarso-conjunctival and skin-muscle flaps. No evidence of recurrence or metastasis was noted 42 months postoperatively. Consideration of such tumors is clinically important, since they have full metastatic potential. We therefore suggest consideration of sweat gland primary mucinous adenocarcinoma in the differential diagnosis of red or violaceous eyelid masses.

Adenocarcinoma, Mucinous↗

[A new type of sweat test and its use in children].

Chloride concentration in sweat was determined by the method of Skine Chloride System of Orion's Model 417 in 100 healthy children and 42 CF children. Statistically significant differences were demonstrated in chloride concentration in sweat between the group of healthy children and CF children. The results of our investigations suggest, that this method is useful in the diagnosis of CF in childhood.

Adolescent↗

Gustatory sweating caused by blunt trauma.

A case of gustatory sweating after blunt trauma to the left side of the face has been discussed. In addition to a fibular fracture, the patient sustained multiple mandibular fractures as well as paralysis of the left facial nerve, which disappeared within five weeks. Three months after the trauma, the patient had gustatory sweating, which was controlled with topical applications of scopolamine hydrobromide.

Adult↗

[An immunohistochemical study of normal sweat glands using an antibody to the breast cyst fluid protein (GCDFP-15)].

In this study, we applied the antibody against the molecular weight 15,000 protein purified from breast cyst fluid (GCDFP-15) to cutaneous tissue, especially to sweat glands. Breast cyst fluid was obtained by needle aspiration from patients with gross cystic disease. DEAE-cellulose column chromatography was performed and followed by SDS-polyacrylamide gel electrophoresis. Antisera against the purified protein were prepared in rabbits. We stained specimens of normal skin by the PAP method. The normal apocrine gland cells were strong positive and eccrine gland dark cells were positive. Eccrine gland clear cells were weak positive or negative. Duct cells of both glands were negative. From these observations, we suggest that this antibody is useful for detecting seromucous cells of sweat glands, including both the apocrine gland cells and eccrine dark cells in the skin.

Animals↗

Sweat gland tumors with stromal haemangiomatous component: an undescribed type of skin tumors. Report of four cases.

An undescribed type of skin tumour, discovered in four cases, is reported. It is characterized by sweat-gland tumours (benign or malignant), with a common stromal haemangiomatous component which confers to it a peculiar pattern. Histogenetic possibilities are discussed. A collision mechanism between a pre-existent hamartomatous component (haemangioma) and a recent sweat-gland growth may be responsible for the appearance of this type and we think that this picture is more frequent but unidentified because, to our knowledge, it was not described up to the present paper.

Adult↗

Effects of thermal stimulation on intracellular sodium, potassium and chlorine in the sweat glands of the cow, sheep and goat.

The intracellular concentrations of electrolyte elements in the sweat glands of the cow, sheep and goat provide evidence of altered secretory activity upon thermal stimulation in the cow but give no indication of such change in the sheep and goat. The results support the view that increased secretory function contributes to the pattern of sweat output in the cow.

Animals↗

Mucinous sweat-gland adenocarcinoma of the inner canthus: a case report.

Mucinous sweat-gland adenocarcinoma is a rare tumor of the eyelid. Fewer than 30 cases have been reported in the literature. We describe the case of a 77-year-old man with a papillomatous lesion of the inner canthus. The lesion was removed, and the histologic examination confirmed the diagnosis of a mucinous sweat-gland adenocarcinoma. After three years, there has been no recurrence or metastasis.

Adenocarcinoma↗

Emotional eccrine sweating. A heritable disorder.

A family with hereditary emotional hyperhidrosis is described. The inheritance pattern is autosomal dominant. A simple quantitative palmar sweat test was used to objectively confirm historical data. Of two family members tested, both had a marked decrease in palmar sweat secretion during administration of diltiazem, a calcium-channel blocker. Additional studies in a large group of patients are needed to extend this observation.

Adolescent↗

[Gustatory sweating following parotidectomy. Frey's syndrome].

398 out of 700 patients (= 56.8%), who underwent parotidectomy in the years 1969-1982 at the ENT-Clinic, University of Münster, answered a follow-up study on the postoperative onset of the auriculotemporal syndrome. We investigated the relations between marked sweating and different surgeons, surgical techniques, histology, lesions of the facial nerve, first appearance and mechanisms to elicit the sweating. The results are discussed with regard to the different hypotheses on the development of Frey's syndrome. No unequivocal indications regarding the cause of Frey's syndrome could be established.

Adolescent↗

[Diagnosis of histidinemia through using the determination of urocanic acid in the sweat by an enzymatic method].

A diagnosis of histidinemia was confirmed by estimation of urokaninic acid in sweat using highly purified preparation of urokaninase from rat liver tissue. The content of urokaninic acid was estimated by the method developed and by means of a known chemical method, which involved reduction of urokaninic acid with zinc in a medium containing HCL; seven children with histidinemia and ten healthy children were examined. The diagnosis of histidinemia was confirmed since the content of urokaninic acid was distinctly decreased in sweat of the imparied children as compared with the control group. The method developed was highly sensitive, reproducible and accurate.

Child↗

The variable prevalence of junctional-complex-associated bodies in the human sweat gland.

Sweat gland biopsies have been examined for their content of junctional-complex-associated (JCA) bodies. These bodies have been observed at the lumen of the gland as well as the intercellular canaliculi. A JCA body was encountered in each of two dark cells with uncondensed secretory granules; one of these cells was from a specimen in which no other bodies could be found after an extensive search. All other JCA bodies occurred in either clear or undifferentiated cells. Junctional-complex-associated bodies occupied pairs of adjacent cells rather frequently, but no cell revealed more than one body. The prevalence of JCA bodies varied widely among specimens. Glands lacking morphologic abnormalities, and others with some atypical features, disclosed few or no such bodies. Some glands that contained JCA bodies also contained coil cells with extensive vacuolization, which occurred as a manifestation of Hurler's disease or on an unexplained basis. Glands from a patient with cystic fibrosis exhibited the highest prevalence of JCA bodies. Some of the coil profiles in glands near a scar in one specimen were composed of undifferentiated cells with rare mitotic figures and no identifiable clear or dark cells. Numerous JCA bodies, particularly at the lumen, were found in sweat glands in this and another specimen containing frequent undifferentiated cells.

Biopsy↗