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[Red tides in México: a review].

With the purpose to make a review on the red tides occurence at mexican coasts, previous studies were analyzed. Dinoflagellates seem to be the main cause of toxic events mainly Gonyaulax polygramma, Gymnodinium catenatum, Pyrodinium bahamense var. compressum and Ptychodiscus brevis. There are other species which cause red tides but are not toxic. They are: Mesodinium rubrum, Gonyaulax triacantha, Noctiluca scintillans, Prorocentrum dentatum, Gymnodinium splendens, G. sanguineum, Ceratium tripos var. ponticum, C. furca, Scrippsiella trocoidea and Oscillatoria erythraea. The first of these is the most common cilliate of the Pacific coastlines and the last one is the most common cyanophyceae during the strong events of "El Niño". The magnitude is quite variable, from small stains of a hundred square meters to several square kilometers and its density ranges from 0.5 to 36 million cells/l. Their residence time goes from 1 or 2 days to as long as 5 months. The great densities are due to Prorocentrum dentatum and P. minimum, the latter is only observed in estuarine ponds for shrimp cultivation, they have not been related to poisoning episodes. New areas of the red tide occurrence have been recorded during the last decades, such as Acapulco Bay, Huatulco, Salinacruz and Puerto Madero. These localities and also Mazatlán, are the only regions in which paralytic shellfish poisoning (PSP) have registered fatal cases and in the Gulf of Mexico a great mortality of fishes has been noted due to ictiotoxin produced by Ptychodiscus brevis. On the other hand, at Yucatan and west coast of the Peninsula of California red tide events are little known.

Animals↗

Overwhelming sepsis with Vibrio vulnificus: a coastal pathogen in Oklahoma.

Vibrio vulnificus has been associated with three main clinical syndromes; primary septicemia; wound infection, and gastroenteritis. This organism has increased virulence for persons with underlying medical conditions that predispose to iron overload or an impaired immune system. Since the organism proliferates more readily in warm, coastal waters, such infections are more commonly found in those regions. Infection can result from the ingestion of contaminated, undercooked seafood; contact of a wound with seawater; or a puncture wound sustained from a contaminated surface. Vibrio infections rarely occur in inland areas, but when they do occur, they are usually a result of the contact of wounds with contaminated, brackish water or the ingestion of raw shellfish. Because infections with this organism occur less frequently in non-coastal regions, the diagnosis may not be suspected initially in susceptible individuals and a delay of treatment may result. We present a case of V. vulnificus sepsis occurring in a man with underlying liver disease and a history of row oyster consumption in Oklahoma and discuss the clinical manifestations of primary sepsis with this organism as well as prevention strategies.

Animals↗

[Some examples of introductions and transfers of mollusks].

Human beings have always introduced non-indigenous species into new environments. Such is the case with shellfish, which the Romans, fine connoisseurs, transferred from France to Italy to mature. The Pacific oyster (Crassostrea gigas) and the Manila clam (Ruditapes philippinarum) are among the most significant examples of these introductions. The author examines the causes, conditions, results, and economic and biological repercussions of these examples. The well known cases of the introduction and implantation of new populations of molluscs are relatively recent, dating back to the beginning of this century. These movements result from an intensification of fishing activities, and the development of the mollusc industry and of transportation. The introduction of fish has been attempted for hundreds of species, whereas the introduction of molluscs has involved no more than a few dozen recorded species. Finally, with regard to the deliberate introduction of molluscs, none has led to significant ecological disruption, but on the contrary, has led to the establishment of permanent coastal activities which do not greatly disturb the natural biotopes.

Animals↗

[International trade in bivalve mollusks and the current situation in France and in Europe].

Over the last century and a half, trade in shellfish has introduced into france four new species intended for aquaculture, as follows: -the Portuguese oyster (Crassostrea angulata) -the American clam (Mercenaria mercenaria) -the Pacific oyster (C. gigas) -the Manila clam (Ruditapes philippinarum). The development of hatcheries and air transport facilities has led to increased trade in these species among the countries of the European Union (France, Ireland, Italy, the Netherlands, Portugal, Spain and the United Kingdom); they are also imported from other countries (Morocco, Tunisia and Turkey). The species involved in this trade are the Pacific oyster, that flat oyster (Ostrea edulis), the Manila clam, the calico clam (R. decussatus) and the scallop (Pecten maximus). The reasons for such trade are economic (as markets and consumption habits differ from one country to another) and ecological (as areas suited to the growth of a species are not always those where natural reproduction is possible). Numerous problems still confront the economic participants, particularly the harmonisation of controls, the fair evaluation of disease risks and the adaptation of administrative procedures to biological constraints.

Animals↗

Prevention of Vibrio vulnificus infections. Assessment of regulatory educational strategies.

CONTEXT: Vibrio vulnificus-related disease caused by eating contaminated raw oysters prompted California to implement regulatory educational initiatives in 1991. OBJECTIVE: To assess California guidelines and education efforts with regard to the prevention of V. vulnificus infections in Los Angeles County and to evaluate compliance with state regulations mandating posting of warning signs concerning risks of eating raw oysters. DESIGN: Review of epidemiologic investigations of V. vulnificus infections in Los Angeles County between 1993 and 1995; telephone interviews of patients or surrogates; and a survey of restaurants serving raw oysters in Los Angeles County. SETTING: General community. MAIN OUTCOME MEASURES: Prior warning of patients and posting of warning signs in restaurants. RESULTS: Recent cases of V. vulnificus infections in Los Angeles County suggest that a unique, vulnerable group (uninsured Hispanic men with viral or alcoholic liver disease) has not been reached through education efforts. Of a total of 11 cases, information regarding being warned about the hazard of eating raw shellfish was available for 8; of these, only 1 case was reported as having been warned, but he had misunderstood the preventive message. Warning signs were not posted adequately in more than 50% of restaurants surveyed and one third of these establishments had signs in English only. Warnings were never located on dining tables and rarely included in menus. CONCLUSION: A more effective strategy is required to prevent V.vulnificus infections in Los Angeles County.

Animals↗

Vibrio vulnificus infections: case reports and literature review.

Vibrio vulnificus is an emerging opportunistic pathogen that is responsible for one of the most fulminant food-borne diseases in immunocompromised hosts. The infection should be suspected in patients with underlying liver disorders who present with a prominent skin rash, hypotension and septic parameters especially if they give a history of seafood or shellfish ingestion. Awareness and early recognition of this infection will result in a better outcome for the patient.

Adult↗

An outbreak of Salmonella enteritidis phage type 19 infection associated with cockles.

An outbreak of infection with Salmonella enteritidis was detected because some of the cases lived near each other. Swift investigation identified cockles as the vehicle of infection. The cockles had been gathered and cooked by a member of the public. Phage typing of the S. enteritidis revealed phage type 19, which is rarely seen in the United Kingdom. The outbreak illustrates the need for care in handling and preparation of shellfish for human consumption. Catering establishments should be wary when offered foodstuffs from unfamiliar suppliers.

Animals↗

Modification of the standard method used in the United Kingdom for counting Escherichia coli in live bivalve molluscs.

The standard method for counting Escherichia coli in live bivalve molluscs is labour intensive and takes three days to obtain a result. Modifications to the standard method were investigated in a collaborative trial conducted in five centres. No significant difference was found between results based on the presence of acid at 24 hours (h) in first stage tests and those based on the presence of acid and gas after 48 h (standard method). The use of the chromogenic medium BCIG (5-bromo-4-chloro-3-indolyl-beta-D glucuronide) agar incubated at 44 degrees C to confirm first stage tests was also found to give equivalent results to conventional confirmation tests. The preferred, modified method removes the presence of gas as a criterion of detection, uses a chromogenic agar medium to confirm the presence of E. coli, and gives results within 48 h. A distribution of simulated samples and selected strains of E. coli to other laboratories using the PHLS external quality assurance scheme for shellfish found no significant difference between results obtained by the standard and modified methods.

Animals↗

Food safety. Federal inspection programs. Council on Scientific Affairs, American Medical Association.

Concern about the safety of the US food supply has stimulated criticism of the present system for assuring safe food in American markets. This report was prepared in response to resolutions introduced at the American Medical Association House of Delegates' December 1990 Interim Meeting. The resolutions requested the AMA to study the plans and procedures needed to improve the federal inspection of meat, poultry, and shellfish. To put these issues into perspective, an overview of food safety is presented. This report is not intended, however, to be a broad review of the Food and Drug Administration's and the US Department of Agriculture's responsibilities for food safety.

American Medical Association↗

A fatal case of Vibrio vulnificus presenting as septic arthritis.

Vibrio vulnificus is an invasive gram-negative bacillus that may cause necrotizing cellulitis, bacteremia, and/or sepsis. Although V vulnificus infection is uncommon, it is frequently fatal and is usually attributed to ingestion of raw shellfish or traumatic exposure to a marine environment; patients are also often found to have a hepatic disorder (cirrhosis, alcohol abuse, or hemochromatosis) or an immunocompromised health status, and most commonly present with septicemia or a wound infection. We describe a patient who presented with septic arthritis as the first clinical manifestation of a V vulnificus infection. The organism was subsequently identified in a synovial fluid aspirate.

Arthritis, Infectious↗

Exposures and outcomes of children with urticaria seen in a pediatric practice-based research network: a case-control study.

OBJECTIVES: (1) To determine the duration, outcome, and associated findings of urticaria in children seen by general pediatricians; (2) to compare the exposure to foods, medications, insect stings or bites, and the presence of other symptoms in children with urticaria with controls; (3) to determine general pediatricians' management of urticaria. DESIGN: Case-control. PARTICIPANTS: Children with urticaria seen by Utah Pediatric Practice Based Research Network members between August 1, 1999, and August 31, 2000. Control patients were matched for age and sex. MAIN OUTCOME MEASURES: Duration of urticaria; associated symptoms; personal and family history of atopy; medications; ingestion of peanuts, nuts, shellfish, tomatoes, strawberries, or eggs; being stung by an insect; suspected cause, diagnostic studies; treatment. RESULTS: Fifty-two cases and 47 controls were enrolled. The mean duration of urticaria was 8.9 days (range, 1-50 days). Seventeen patients (33%) and 1 control patient were taking antibiotics (odds ratio [OR], 22.3; 95% confidence interval [CI], 2.8-176; chi(2), P<.001). Fourteen patients and 5 controls had gastrointestinal symptoms (OR, 3.1; 95% CI, 1.02-9.4; chi(2), P =.04). There were no differences between cases and controls for other symptoms, personal or family history of atopy, ingestion of the foods listed, insect sting, or other medications. A cause was suspected in 28 patients (54%): a "viral illness" (19%), antibiotics (15%), or a combination (35%). CONCLUSIONS: Patients were more likely than controls to be taking an antibiotic and were more likely to have a personal or family history of atopy or to report ingesting foods commonly associated with urticaria. A viral illness was the most common cause suspected by pediatricians.

Adolescent↗

Fish intake, contaminants, and human health: evaluating the risks and the benefits.

CONTEXT: Fish (finfish or shellfish) may have health benefits and also contain contaminants, resulting in confusion over the role of fish consumption in a healthy diet. EVIDENCE ACQUISITION: We searched MEDLINE, governmental reports, and meta-analyses, supplemented by hand reviews of references and direct investigator contacts, to identify reports published through April 2006 evaluating (1) intake of fish or fish oil and cardiovascular risk, (2) effects of methylmercury and fish oil on early neurodevelopment, (3) risks of methylmercury for cardiovascular and neurologic outcomes in adults, and (4) health risks of dioxins and polychlorinated biphenyls in fish. We concentrated on studies evaluating risk in humans, focusing on evidence, when available, from randomized trials and large prospective studies. When possible, meta-analyses were performed to characterize benefits and risks most precisely. EVIDENCE SYNTHESIS: Modest consumption of fish (eg, 1-2 servings/wk), especially species higher in the n-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), reduces risk of coronary death by 36% (95% confidence interval, 20%-50%; P<.001) and total mortality by 17% (95% confidence interval, 0%-32%; P = .046) and may favorably affect other clinical outcomes. Intake of 250 mg/d of EPA and DHA appears sufficient for primary prevention. DHA appears beneficial for, and low-level methylmercury may adversely affect, early neurodevelopment. Women of childbearing age and nursing mothers should consume 2 seafood servings/wk, limiting intake of selected species. Health effects of low-level methylmercury in adults are not clearly established; methylmercury may modestly decrease the cardiovascular benefits of fish intake. A variety of seafood should be consumed; individuals with very high consumption (> or =5 servings/wk) should limit intake of species highest in mercury levels. Levels of dioxins and polychlorinated biphenyls in fish are low, and potential carcinogenic and other effects are outweighed by potential benefits of fish intake and should have little impact on choices or consumption of seafood (women of childbearing age should consult regional advisories for locally caught freshwater fish). CONCLUSIONS: For major health outcomes among adults, based on both the strength of the evidence and the potential magnitudes of effect, the benefits of fish intake exceed the potential risks. For women of childbearing age, benefits of modest fish intake, excepting a few selected species, also outweigh risks.

Animals↗

Transfer of foreign gene to giant freshwater prawn (Macrobrachium rosenbergii) by spermatophore-microinjection.

We developed a spermatophore-microinjection (SMI) technique that allows exogenous DNA fragments to be transferred easily into the giant freshwater prawn (Macrobrachium rosenbergii), an important aquacultural shellfish and aquatic invertebrate model. From 28 to 1, 000 ng of the circular plasmid pGL, in a total volume of 1 microl, were directly microinjected into spermatophores. Fertilization and hatching of prawns created with SMI were completed in vivo. Fertilization and hatching rates in the SMI treatments did not differ from those of the untreated control group. The genomes of free swimming, SMI-created larvae (21 days after fertilization) were analyzed using PCR and Southern blot analyses. A product with a molecular mass of 680 bp was amplified. It corresponded to amplifications of pGL, and Southern blot analysis revealed that the amplified band was positive. The gene transfer rate was primarily dependent on the concentration of DNA during SMI. The higher the concentration of pGL, the higher the rate of gene transfer. PCR and Southern blot analyses detected the existence of foreign DNA in 16 of 23 samples (70%) of genomic DNA isolated from hatched larvae in the 750 ng pGL SMI treatment. SMI, described here for the first time, is the simplest and most efficient method for mass producing transgenic giant freshwater prawns.

Animals↗

Toxicologic evaluation of yessotoxin.

Yessotoxin (YTX), originally found in association with diarrhetic shellfish poisoning (DSP), caused neither intestinal fluid accumulation nor inhibition of protein phosphatase 2A. Orally, YTX was not lethal to mice at 1.0 mg/kg. The toxin showed weak cytotoxic and antifungal activities. Neither hemolytic nor ichthyotoxic effect was observed.

Animals↗

Evaluation of the use of two human cell lines for okadaic acid and DTX-1 determination by cytotoxicity assays and damage characterization.

Two human cell lines have been used, HEp-2 and (de)differentiated Caco-2, derived from a larynx and a colon carcinoma, respectively, with the aim of evaluating and characterizing the cytotoxicity of okadaic acid (OA) and related toxins. Effects of OA and dinophysistoxin-1 (DTX-1) on cell viability (neutral red uptake) and on cell morphology/cytoskeleton structure have been observed in both cell lines, though at different time exposures and with different concentrations. The morphological alteration was detected earlier than the viability inhibition in HEp-2 cells with both toxins and in Caco-2 cells with DTX-1. HEp-2 cells have shown to be more sensitive than the intestinal cell line and thus possibly suitable for screening of contaminated samples, while Caco-2 cells could be used for further investigating the possible mechanisms involved in diarrhoeic shellfish poisoning (DSP) toxins.

Apoptosis↗

Preparation and characterization of DNA films induced by UV irradiation.

Large amounts of DNA-enriched materials, such as salmon milts and shellfish gonads, are discarded as industrial waste. We have been able to convert the discarded DNA to a useful material by preparing novel DNA films by UV irradiation. When DNA films were irradiated with UV light, the molecular weight of DNA was greatly increased. The reaction was inhibited by addition of the radical scavenger galvinoxyl suggesting that the DNA polymerization with UV irradiation proceeded by a radical reaction. Although this UV-irradiated DNA film was water-insoluble and resistant to hydrolysis by nuclease, the structure of the DNA film in water was similar to non-irradiated DNA and maintained B-form structure. In addition, the UV-irradiated DNA film could effectively accumulate and condense harmful DNA-intercalating compounds, such as ethidium bromide and acridine orange, from diluted aqueous solutions. The binding constant and exclusion number of ethidium bromide for UV-irradiated DNA were determined to be 6.8 +/- 0.3 x 10(4) M(-1) and 1.6 +/- 0.2, respectively; these values are consisted with reported results for non-irradiated DNA. The UV-irradiated DNA films have potential uses as a biomaterial filter for the removal of harmful DNA intercalating compounds.

Acridine Orange↗

Small round viruses: classification and role in food-borne infections.

Since the first observation of Norwalk virus in the electron microscope in 1972, many different small virus particles in the size range 20-40 nm have been described world-wide in association with outbreaks of gastroenteritis. Progress characterizing these agents has been hampered by the relatively small numbers of particles present in clinical material and the lack of success in culturing them. Although the relationship between some of these viruses remains confusing, a number of distinct groups has emerged, based on morphological features and limited physical data. Immuno-electron microscopy has proved valuable in detecting viruses but the addition of antibody can mask surface morphological features. Examination of viruses in negatively stained preparations without added antibody has revealed distinct morphological differences and viruses previously thought to be simply antigenic variants within the Norwalk group of viruses clearly belong to other groups. Preliminary evidence suggests that one human virus unrelated to Norwalk has a single-stranded DNA genome and is a parvovirus. Some groups have been implicated in outbreaks of food-borne gastroenteritis, particularly after the consumption of shellfish, and their role in other food-borne and water-borne outbreaks is being increasingly recognized.

Caliciviridae↗

Prehistoric "fish-eaters" along the eastern Arabian coasts: dental variation, morphology, and oral health in the Ra's al-Hamra community (Qurum, sultanate of Oman, 5th-4th millennia BC).

The Ra's al-Hamra prehistoric fishermen lived in isolation on the Qurum rocky promontorium in Oman during the 5th-4th millennia BC. To date, they represent the most ancient and numerous human fossil group excavated from the Arabian peninsula. Like other contemporaneous archaeologically documented small communities along the desert Arabian coasts, they intensively exploited ocean resources and collected molluscs from nearby mangrove swamps. The present study analyzes aspects of dental anthropology (including crown variation, morphology, dental wear, and oral health), in 600 permanent teeth from 49 individuals of both sexes excavated at the Mesolithic RH5-site by the Italian Archaeological Mission in Oman from 1981 to 1985. In association with a general low degree of morphometric variation, the Ra's al-Hamra dental crowns show low sexual dimorphism and are consistently reduced in size. These features are unexpected in a preagricultural population, especially when these data are compared to other eastern African and near Middle Eastern prehistoric populations. These data are discussed within the general context of human dental structural reduction occurring during the post-Pleistocene and are interpreted according to the "increasing population density effect" model. There are other significant differences that characterize the Ra's al-Hamra dentitions with respect to both eastern and western prehistoric human groups. The frequency of numerous nonmetric crown traits supports the hypothesis that a microdifferentiation phenomenon occurred in this marginal area. The preliminary skeletal analysis and the palaeodemographic profile show that the Omani prehistoric fishermen were affected by genetic isolation and inbreeding as well as strong environmental stress. Because of the grit assimilated with dried fish and the high shellfish consumption, dental wear was extreme in all age groups at Ra's al-Hamra and occasionally was responsible for serious hematogenously spread infections. In spite of the great anthropological importance of ancient Arabian populations, very few studies on skeletal and dental samples have been completed. The present paper offers an odontological data set for future comparative research in the area.

Adult↗