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PubMed1999

[Emergence of resistance to macrolides in Streptococcus pyogenes].

BACKGROUND: Diseases produced by Streptoccocus pyogenes are still a problem in Chile, as in the rest of the world. It exhibits in vitro susceptibility to different antimicrobials, but penicillin continues to be the treatment of choice. Alternative drugs have been developed for allergic patients, such as erythromycin, new macrolides and cephalosporins. Nevertheless, resistant strains are appearing due to the indiscriminate use of macrolides. AIM: To assess present antimicrobial susceptibility of S Pyogenes strains isolated from chilean patients. MATERIAL AND METHODS: The susceptibility to penicillin, macrolides, clindamycin, cephalotin and vancomycin of 153 S Pyogenes strains, obtained from different health centers of the Metropolitan Region and isolated between 1996 and 1998, was assessed using the Kirby-Bauer method. Agar dilution minimal inhibitory concentration was then determined to macrolide resistant strains. RESULTS: All strains were susceptible to penicillin. There was a 7.2% cross-resistance to macrolides. CONCLUSIONS: These results confirm that S Pyogenes resistance to macrolides has increased considerably in the Metropolitan Region of Chile during the last years.

Anti-Bacterial Agents↗
PubMedDate not supplied

[Incidence of Streptococcus pyogenes pharyngitis in Bariloche, Argentina].

The incidence and seasonality of pharyngitis by S. pyogenes in Bariloche, a city where long periods of low temperatures result in extended indoor activities were studied. A total of 5276 throat swab specimens collected during 2000-2003 in the clinical microbiology laboratories of the three main medical institutions of the city, were analyzed. Samples were cultured on blood-agar media containing 5% defibrinated sheep blood, and incubated for 24-48 h at 35 degrees C. Strains were identified using standard procedures. Monthly means for throat swabs, S. pyogenes isolates, and percent of S. pyogenes pharyngitis, were estimated. The incidence of pharyngitis by this microorganism was greater than 24% for every month within the April-December period, reaching a maximum in November (33%). February was the month with the lowest incidence (13%). It was observed an increasing trend since March through November with a through in July, and a marked decrease that began in December, reaching its lowest rate in January and February, the warmest months. This seasonal pattern differs from those observed for temperate climates. The incidence of S. pyogenes pharyngitis in Bariloche was high through the greater part of the year, since about the middle of autumn to the beginning of summer.

Adult↗
PubMed1996

Lectin mediated binding of Streptococcus pyogenes to human oropharyngeal mucosal epithelial cells.

The study was carried out to determine the role of lectins and sugars in the adhesion of S. pyogenes to human pharyngeal and buccal epithelial cells. In vitro adhesion assay has shown that Con A and Dolicos biflorus lectins inhibited the attachment of S. pyogenes to the oropharyngeal mucosal cells. Among different sugars used, N-acetyl-D-galactosamine and D-galactose have significantly blocked the binding of streptococci to PEC and BEC. These findings indicate that lectins and sugar molecules mediate the adhesion of S. pyogenes to the pharyngeal epithelial cells which may be important in the cellular pathogenesis of streptococcal infections which originate at the human oropharyngeal mucosa.

Epithelial Cells↗
PubMed1998

[Sensitivity to beta lactams and macrolides of Streptococcus pyogenes isolated at the services from 1994 to 1998].

The identification and antibiotic sensibility of 188 strains of streptocoques pyogenes had been performed from 1994 to March 1998. We were interested on active antibiotics against streptocoques pyogenes: beta lactamins (penicillin-ampicillin) and macrolids (erythromycin). The aim of this study is to reveal resistances and calculate percentages of resistant strains to these antibiotics. The WHO recommend to treat streptococcal sore throat with penicillin, and with erythromycin when there is a proved inhibility to the penicillin. No penicillin resistant streptocoques pyogenes has been found in the world, that concords with our study: all strains (188 sp.) are penicillin sensitive. Macrolids resistant strains of streptocoques pyogenes has been described by a lot of countries with different distributions. In our study global percentage of resistant strains to erythromycin is 24% and 29% for throat specimen.

Anti-Bacterial Agents↗
PubMed2003

Purification of recombinant non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Streptococcus pyogenes expressed in E. coli.

Streprococcus pyogenes gapN was cloned and expressed by functional complementation of the Escherichia gap mutant W3CG. The IPTG-induced NADP non-phosphorylating GAPDH (GAPN) has been purified about 75.4 fold from E. coli cells, using a procedure involving conventional ammonium sulfate fractionation, anion-exchange chromatography, hydrophobic chromatography and hydroxyapatite chromatography. The purified protein was characterised: it's an homotetrameric structure with a native molecular mass of 224 kDa, have an acid pI of 4.9 and optimum pH of 8.5. Studies on the effect of assay temperature on enzyme activity revealed an optimal value of about 60 degrees C with activation energy of 51 KJ mole(-1). The apparent Km values for NADP and D-G3P or DL-G3P were estimated to be 0.385 +/- 0.05 and 0.666 +/- 0.1 mM, respectively and the Vmax of the purified protein was estimated to be 162.5 U mg(-1). The S. pyogenes GAPN was markedly inhibited by sulfydryl-modifying reagent iodoacetamide, these results suggest the participation of essential sulfydryl groups in the catalytic activity.

Chemistry, Physical↗
PubMed2004

[Secondary Streptococcus pyogenes peritonitis following necrotizing fasciitis].

We report the case of a 66-year-old woman with diabetes mellitus and disseminated lupus treated with immunosupressive drug. She was admitted for an inflammatory oedema of the right lower limb associated to diffuse abdominal pain and vomiting. The occurrence of septic shock with multiple organ failure and surgical abdominal picture led us to perform urgent laparotomy without taking in account the evolutive signs of cutaneous lesions i.e. purpuric elements and haemorrhagic phlycthena. The abdominal cavity exploration showed peritonitis without digestive tracts perforation. The patient died few hours after surgical procedure. All bacteriological samples i.e. peritoneal effusion, blood cultures and phlycthena liquid were positive for Streptocoque pyogenes (group A) and peritonitis was considered to be related to haematogen diffusion from rapid course necrotizing fasciitis.

Aged↗
PubMed1999

Role of keratinocyte injury in adherence of Streptococcus pyogenes.

Keratinocytes injured acutely by UVB light or lipopolysaccharide were used to test the hypothesis that keratinocyte injury promotes bacterial adherence and the development of group A streptococcal skin infections. Injury did not affect adherence to undifferentiated and differentiated keratinocytes, but keratinocyte differentiation promoted adherence four- to fivefold.

Bacterial Adhesion↗
PubMedDate not supplied

[Study of antibiotic sensitivity of Streptococcus pyogenes isolated in the hospital milieu (Charles Nicolle hospital of Tunis)].

The purpose of the present work was to investigate the susceptibility of S. pyogenes to antimicrobial agents. The strains have been taken from the bacteriological Service of Charles Nicolle's hospital at Tunis, and that during the period from January 1988- to December 1990. The antibiogram results as well as the minimal inhibitrice concentration determined by the agar diffusion technique, reveal that S. pyogenes continues to be very susceptible to the beta-lactamins (M.I.C. of penicillin G is "0.15 for 50% of strains tested. Resistance are noted for chloramphenicol; tetracycline and novobiocine (13%, 93%, 80%) not considered as good antibiotics anti-streptococcies. The macrolids are effective against S. pyogenes (100% of susceptibility) that's they are considered as the best alternating antimicrobiologents to the beta-lactamins (M.I.C. of erythromycin is 0.015.

Anti-Bacterial Agents↗