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PubMed1971

Serological responses and results of natural infectious challenge of recipients of zonal ultracentrifuged influenza A2-Aichi-2-68 vaccine.

In an epidemic of Hong Kong influenza in a penal institution, acute respiratory illness occurred in 5.6% of 196 inmates given single subcutaneous doses of 332, 535, or 1 265 CCA of zonal centrifuged, inactivated vaccine and in 11.7% of 755 nonvaccinated inmates (P<0.03). The frequency of illness among inmates who received single doses of 54 or 137 CCA of vaccine was not significantly different from that among the nonimmunized inmates (chi(2)=0.93, not significant). The vaccine was prepared with the A2/Aichi/2/68 strain of influenza and administered 7 weeks before the outbreak. Virus shedding was significantly lower in vaccinated inmates with respiratory disease (11.1%) than in nonvaccinated inmates (73.7%, P<0.04).Serological studies showed an increasing mean titre and increasing frequency of 4-fold or greater haemagglutination inhibition and neutralizing antibody responses with increasing dosage of vaccine.

Antibody Formation↗
PubMed1982

Theoretical and ultracentrifugal analysis of immune complex formation between monoclonal antibodies and human IgG.

Immune complex formation of four different mouse monoclonal antibodies against human IgG has been studied using analytical zonal centrifugation. A theoretical model has been used to depict thermodynamic ideal immune complex formation of monoclonal antibodies. It was found that the four monoclonal antibodies differed very much with respect to immune complex formation with human IgG. One of the monoclonal antibodies formed immune complexes in agreement with the theoretical model. Another was strongly related thereto. A third monoclonal antibody formed only a single complex and might exhibit a positive co-operativity between its two sites. A fourth formed as excess of a possibly cyclic complex. Thus monoclonal antibodies differ substantially with respect to physical properties adding a new aspect to the problems of antibody heterogeneity. It is moreover found that analytical zonal centrifugation can be used to estimate the number of antigenic determinants and antibody-binding sites thereby assuring whether or not a particular IgG monoclonal antibody has two binding sites.

Antibodies, Monoclonal↗