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PubMed1992

The influence of membrane charge on the kinetic properties of Na,K-ATPase.

Lipophilic ions modify the affinity of the cation binding sites of the membrane-bound Na,K-ATPase. We studied the effect of the lipophilic ions tetraphenyl-phosphonium (TPP+) and tetraphenylboron (TPB-) on the binding of Na+ and K+ to the cation site(s) that are exhibited by the enzyme during the catalytic cycle: the high-affinity (inside) Na-binding site, site I, the low-affinity (outside) Na-leaving site, site II, and the high-affinity (outside) K-site, site III. Site I: In the presence of TPP+ (positive charge added to the lipid environment) a higher Na(+)-concentration was needed to obtain phosphorylation of the enzyme, whereas in the presence of TPB- (negative charge added to the lipid environment) phosphorylation was obtained at a lower Na(+)-concentration, but the change in apparent K0.5 for Na+ was small, (K0.5Na,TPP = 0.180 mM and K0.5Na,TPB = 0.07 mM), indicating only a minor influence of membrane charge on Na+ binding to site I. Site II: Compared to control conditions, more Na+ was required to inhibit ATP-hydrolysis and to increase the steady-state level of ADP-sensitive phosphoenzyme when TPP+ was present, and the opposite was observed with TPB-, indicating a strong influence of membrane charge on the Na+ occupancy of site II. Site III: TPP+ induced a significant decrease both in the rate of K-dependent dephosphorylation of preformed E32P and in the K+ affinity. The effect of TPP+ on the ATP hydrolysis rate strongly resembled the effect of decreasing [KCl]. The results indicated a pronounced effect of adding positive charge to the lipid environment of site III.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenosine Diphosphate↗
PubMed1992

[Effects of fertilization on yield of Asparagus cochinchinensis (Lour.) Merr].

The experiment revealed that the use of various kinds of fertilizers has apparent effect on the yield of Asparagus cochinchinensis during its growth period. Nitrogen fertilizer and compound fertilizer are suitable for the seeding period, while compound fertilizer, phosphorus and potassium fertilizers are suitable for the period of root forming. The quantity of fertilizer to be used depends on the growing and soil conditions.

Fertilizers↗
PubMed1963

The toxicity of some organophosphorus compounds to adult Anopheles stephensi.

The authors have evaluated a number of organophosphorus compounds for residual contact toxicity to adult Anopheles stephensi. Fenthion and malathion were the most promising of the compounds, and wettable powder deposits at a dosage of 1 g/m(2) on plywood remained effective for five months. There was, however, a rapid loss of effectiveness on dried mud bricks stored at 25 degrees C and 50%-55% relative humidity.Diazinon and ronnel were less persistent on plywood. Guthion and coumaphos, although highly toxic by topical application, were both ineffective as contact insecticides when applied as solids in suspension. Trithion and methyl trithion were relatively low in toxicity both by topical application and as contact insecticides.

Animals↗