SEARCH · PubMed Health

Results for “IDENTIFICATION”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,531 records · Page 85Linked to original sources

PubMed1979

[Identification of the enterobacteria isolated from patients by using the Enterotube test system].

The Enterotube test system intended for the accelerated identification of enterobacteria was used to study 319 strains isolated from patients with suppurative complications after surgical operations and traumatic lesions. The test system was found to allow a reliable identification of Enterobacteriaceae having characteristic biochemical properties. It was also found that the kit intended for 10 tests could prove insufficient for the identification of atypical bacteria. The Enterotube test system made it possible to differentiate 95% of enterobacterial strains; in the identification of bacteria by their genus and species and results obtained with the use of the Enterotube test system and common biochemical methods were found to be identical (in 96.4% of tests).

Bacteriological Techniques↗

PubMed1985

Instruments for microbial identification.

Microbiology instruments can provide rapid and accurate identification of gram-negative bacilli. The three instruments currently in widespread use vary in accuracy of identification, types of organisms identified, speed of identification, cost, and degree of automation. If matched to the needs of individual laboratories, microbiology instruments can enhance the quality and efficiency of bacterial identification.

Bacteria↗

PubMed1985

Evaluation of the API 20 STREP system for species identification of "viridans" streptococci isolated from bacteremia.

Species identifications of 71 strains of viridans streptococci isolated from blood and 4 reference strains were made by the API 20 STREP system (API system S. A., Montalieu-Vercien, France) and the conventional method. There are high levels of agreement between results obtained with the both methods for determining acidification from carbohydrate except inulin. The API 20 STREP system correctly identified 74.7% of the viridans streptococci with 9.3% low descrimination, 12% incorrect and 4% unidentified. All strains of S. mitis, S. mutans, S. salivarius and S. anginosus-constellatus were correctly identified. The correct identification rates for S. sanguis I, S. sanguis II and S. MG-intermedius were 88.9%, 68% and 61% respectively. The difference of inulin reaction and the taxonomy discrepancy may be the cause of different identification. The study indicates that the API 20 STREP system has a good potentiality for species identification of viridans streptococci at present time, however, further refinement in needed.

Bacteriological Techniques↗

PubMed1982

Office laboratory identification of Neisseria gonorrhoeae.

Current literature is contradictory regarding what tests are necessary to establish an adequate presumptive diagnosis for office identification of Neisseria gonorrhoeae. This is especially true in light of recent reports of N meningitidis causing acute genital infection. This study was designed to look at the various criteria recommended and to establish guidelines for the office identification of N gonorrhoeae. Four hundred thirty-six isolates grown on Thayer-Martin selective agar were studied. Of these, 156 were found to be oxidase-positive. Gram stain of the isolates showed that 31.4 percent of the oxidase-positive isolates were gram-negative diplococci, 59 percent were yeastlike mole, and 9.7 percent were gram-negative bacilli. All of the gram-negative diplococci were confirmed to be N gonorrhoeae by sugar fermentation studies. No isolates of N meningitidis or saprophytic Neisseria were found. On the basis of this finding, it is recommended that office identification of N gonorrhoeae from genital or anal cultures should include (1) growth on Thayer-Martin (or comparable) medium, (2) positive reaction to oxidase reagent, and (3) identification of gram-negative diplococci on Gram stain of the Thayer-Martin isolate.

Anal Canal↗

PubMed1984

A coding system for a computer aided dental identification based on the "Canobrain Programme".

Three situations requiring the identification process are considered, that is, (Situation 1) identification of air disaster victims, (Situation 2) identification of an unknown body, and (Situation 3) identification of a missing person. In this paper, a coding system using "Canobrain Programme" in each situation is described. It employs 3 digits and 2 letters in situation 1 or 4 digits in situations 2 and 3 to designate the status of a tooth surface and the nature of the dental materials used in any restoration. Illustrative examples of the trial of this system in situation 1 and 2 are set out.

Accidents, Aviation↗

PubMed1982

[Identification of Gram negative rods and evaluation of their antibiotic sensitivity: comparison of the BIOTEST MHK/ID-system with the API 20E-system and the agar diffusion test].

BIOTEST MHK/ID is a new system for the identification of gramnegative fermentative and non fermentative rods and for the simultaneous determination of the minimal inhibitory concentrations (MICs) against 15 antimicrobial drugs. Its identification system was compared with the API 20E kit. When the computer generated identification manuals for each of the systems were used 99.5% of the 190 strains tested were identified. Of these identifications 98.4% were identical. Comparing the results of altogether 16 biochemical reactions 92.3% (table 4) were conformable. Considering each single test low agreement was found with the results of the Citrate-, Urea-, Voges-Proskauer- and Arginindehydrolase-tests (76.8, 79.5, 85.3 and 86.8% agreement, respectively). The BIOTEST MHK/ID system yielded more positive reactions. But despite of these differences there was no influence on the accuracy in identifying the species. Thus, both systems must be considered equally suited to identify gramnegative rods. The results of both BIOTEST MHK/ID system (MIC-values) and the agardiffusion test (inhibition zone sizes) were transferred into the interpretative criteria "sensitive", "intermediate" and "resistant" (table 1) and compared for each strain. The overall-agreement amounted to 93.0% (table 7). With the MIC-test, however, more (7.2%) strains were found "intermediate" than with the agardiffusion test (3.1%, table 8).

Anti-Bacterial Agents↗

PubMed1981

Separation and identification of equine leukocyte populations and subpopulations.

Various methods of separation and identification of major equine leukocyte populations and subpopulations were used. The purity of T and B lymphocytes separated in Sephadex anti-equine F(ab')2 columns was 87% to 99% and 83% of 97%, respectively. The purity of T lymphocytes separated in nylon-wool columns was 89% to 98%. Preparations of B lymphocytes separated in glass-bead columns were 68% to 79% pure. The presence (or absence) of surface immunoglobulin by immunofluorescence was the most consistent and reliable method for the identification of B or T lymphocytes, respectively. However, the erythrocyte-antibody-complement-rosette method for the identification of B cells and the erythrocyte-rosette method for the identification of T cells were not suitable. Monocytes were separated by the adherence method, and the purity, as identified by the latex particle ingestion procedure, was 70% to 78%. Electron microscopy of monocytes stained by peroxidase activity did not identify these cells. The purity of neutrophils obtained by the Ficoll-Hypaque separation method was 95% to 97%. The merits and usefulness of these methods were discussed.

Animals↗

PubMed1980

[Comparison of Micro-ID, API 20 E and a conventional technique for the identification of Enterobacteriaceae].

A new system, Micro-ID, for the identification of Enterobacteriaceae is described. 100 strains of Enterobacteriaceae (Table I) are identified by Micro-ID and the identifications are compared with the identifications performed by conventional techniques and by the API 20 E system. There is a total agreement between Micro-ID and conventional techniques on 96.5% and between API 20 E and conventional techniques on 90% (Tables II and III). The differences in the reactions between the systems are discussed and explained. It is concluded, that the Micro-ID and the API 20 E both are very suitable for the identification of Enterobacteriaceae.

Bacteriological Techniques↗

PubMed1980

[Rapid identification of Enterobacteriaceae with the Micro-ID system (author's transl)].

Micro-ID, a new kit system for rapid identification of Enterobacteriaceae, was compared with a conventional media-system, the extended Enterotube system (completed with arabinose, rhamnose and the Voges-Proskauer reaction), and partially with the API-20E system. When the computer generated Identification Manual for Micro-ID was consulted the system showed complete agreement at the genus level, but differed in 5% at the species level. Problems arose from misidentifications by nonfermenting organisms. 15 turbid blood cultures with gramnegative rods were tested additionally. In 11 cases the Micro-ID provided correct identification of the species and in further 3 cases of the genus. In summary Micro-ID is easy to handle and an accurate, convenient kit for the short-term identification of Enterobacteriaceae.

Bacteriological Techniques↗

PubMed1982

The identification of Linda Agostini: the significance of dental evidence in the Albury Pyjama Girl case.

The partly burned and battered body of a young woman was found in a culvert near Albury, New South Wales, Australia, on September 1, 1934. Although identification was delayed for 10 years, it was finally achieved by dental comparison, following which the crime was quickly solved and the woman's husband brought to trial for murder. The mystery surrounding this bizarre case attracted international attention at the time. The dental identification was hotly disputed and even today is a subject of controversy. This paper briefly reviews some of the circumstances that contributed to the long delay in establishing identification and contrasts the identification procedures used then with those available now. Some important lessons may be learned from the experience of this case that have particular relevance to forensic odontology today. Apart from occasional references to this case in the scientific literature, a number of stories about it have been published in the popular press from time to time. The most recent, reliable, and comprehensive account was published in 1978 in a book by Robert Coleman entitled The Pyjama Girl. This paper has drawn heavily from Mr. Coleman's research, as well as from original court files.

Adult↗

PubMed1980

[Identification of the proteins in white brined and yellow cheese].

Experiments were carried out to establish a method for the identification of proteins in white brined cheese and kashkaval, made of sheep and cow milk or a mixture of them in different proportions. It was proved that the application of a polyacryl amid electrophoresis under definite conditions can secure a species identification of the proteins of the white brined cheese and the kashkaval made of sheep and cow milk. It was proved also that the protein spectra of the white brined cheese and the kashkaval, made of the two sorts of milk, differed both as to the amount of the fractions and subfractions obtained and as to their electrophoretic mobility--Rx. The method offered enables an objective identification of the species composition of proteins in white brined cheese and in kashkaval as well as the identification of the proportions between the sheep and the cow milks in the dairy products obtained.

Animals↗

PubMed1978

[Segmental anatomic diagnosis in congenital cardiac anomalies. I. Anatomic criteria for the identification of the cardiac cavities (author's transl)].

Anatomical features are illustrated for the identification of the cardiac chambers. Essential and accessory morphological structures are recognized. The identification of the atrial cavities is based upon the anatomy of the free walls (crista terminalis, size and shape of the atrial appendages). On the contrary, the identification of the ventricles is founded on the septal morphology (trabecula septo-marginalis on the morphological right side, smooth basal septum on the morphological left side, presence or absence of septal insertions of the atrio-ventricular value apparatus). Finally, no essential feature can be applied to the identification of the great arteries.

Heart↗

PubMed1994

[Evaluation of the usefulness of commercial systems Quantum II and Rapid ID 32E for quick identification of rods from the Enterobacteriaceae family].

The usefulness of the commercial systems Quantum II (Abbott) and Rapid ID 32 E (bioMérieux) for quick identification of Enterobacteriaceae has been evaluated. The study was carried out on strains representing 12 genera of Enterobacteriaceae identified with the aid of classical biochemical tests. The Quantum II system was evaluated in 1985 using 386 strains and the Rapid ID 32 E system in 1992 using 106 strains. When the Quantum II system was used, 237(61.4%) strains were classified correctly in the species, 58(15.0%) were unidentified and 91(23.6%) were identified incorrectly. The Rapid ID 32 E system made possible correct identification of 63(59.4%) strains to the species (in the case of Salmonella rods to the subgenus) and 80(75.5%) strains to the genus. Incorrect identification results were most often obtained in the case of Shigella and Yersinia rods, Salmonella rods of subgenus I fermenting lactose and Enterobacter. The results show that complying with inoculum tests (0.5 according to the McFarland scale) as recommended by the manufacturer and a four-hour incubation, when investigating some Enterobacteriaceae strains which multiply more slowly or exhibit a weaker metabolic activity, does not ensure optimal conditions for the appearance of a set of biochemical features essential for strain identification.

Bacterial Typing Techniques↗

PubMed1994

[Utility of the direct culture examination (Ziehl-Neelsen) using the Bactec system for the presumptive identification of Mycobacterium tuberculosis complex, Mycobacterium avium complex, Mycobacterium xenopi, and Mycobacterium kansasii].

BACKGROUND: The usefulness of the microscopic examination of Bactec 12B and 13A growth medium as a method for the possible identification of M. tuberculosis complex, M. avium complex, M. xenopi, and M. kansasii was performed out to guide the selection of different genetic identification probes and, in the case of M. xenopi, the selection of the temperature of subcultures incubation. METHODS: Upon detection of an index of growth greater than 100 in Bactec tubes, staining was performed by the Ziehl-Neelsen technique. On the basis of the morphology observed, the possible identification was performed by genetic probes. Subcultures were used for definitive identification. RESULTS: Three hundred forty-four positive samples were studied by radiometric technique. A total of 190 strains were identified as M. tuberculosis, 88 strains as M. avium-intracellulare (MAI), 33 strains as M. xenopi, 14 strains as M. kansasii and 19 strains were identified as: M. gordonae (10), unpigmented rapid growth microbacteria (7), and M. simiae (2). Sensitivity, specificity, positive predictive value, and negative predictive value were 97.9%, 95.4%, 96.4%, and 97.3%, respectively for M. tuberculosis complex, 84.0%, 99.2%, 97.3% 94.7% for M. avium complex; 63.6%, 98.3%, 80.7%, 96.2% for M. xenopi; 35.7%, 98.1%, 45.5% 97.2% for M. kansasii. CONCLUSIONS: The morphology of M. tuberculosis complex examined in the radiometric system in useful to differentiate this species from other microbacteria (MOTT), allowing the selection of specific probe used. Within the MOTT, M. avium complex also has morphological characteristics which are useful for its differentiation, the morphology usually described for the remaining species was frequently not observed.

Bacteriological Techniques↗

PubMed1994

[Identification of yeasts of the Candida genus with a growth inhibition system: Microring YT].

BACKGROUND: It has been observed an spectacular increasing of opportunistic Candida yeast infections. Many of them are fatal, and rapid and effective identification of the infecting species contributes to start the correct treatment. Several new methods for yeast identification have become available; Microring YT is one of these methods based on the growth inhibition by 6 different chemical products. The aim of this work is to study the performance of the test using representative clinical yeast isolates. METHODS: A total of 146 strains belonging to the 5 most common Candida species isolated in the clinical laboratory were identified using conventional methods (germ tube and chlamydospores production, and the standard API 20C AUX and 16 sugars auxonography; Institute Pasteur) and the Microring YT System. This test uses the differing susceptibilities of yeast to 6 discs mounted on a filter paper ring. The chemical products and dyes are: janus green, ethidium bromide, triphenyl tetrazolium chloride, brilliant green, cycloheximide and rhodamine 6G. The inhibition pattern of a 6 digit code is compared with a list of profiles. RESULTS: Using the Microring YT system 112 of the 146 studied strains were correctly identified with an overall concordance of 77% between this method and the standard one. The morphological study (germ tube production) increased 6% the identification of Candida albicans. Better results were obtained with C. krusei and C. parapsilosis (85% of concordance). With C. glabrata only 59% of concordance was found. CONCLUSIONS: In spite Microring YT is a simple method, easy to perform and read, it was considered inadequate for the identification of Candida species as a routine microbiological procedure.

Bacteriological Techniques↗

PubMed1994

[Rapid identification of mycobacteria by combined method of polymerase chain reaction and the gen-probe DNA hybridization system].

We developed the rapid detection and identification method of mycobacteria, involving amplification of mycobacterial 16S rRNA gene by nested PCR and identification of M. tuberculosis complex or M. avium-intracellulare complex (MAC) by hybridization protection assay (HPA) using the acridinium-ester (AE) labeled DNA probe. The specificity of the nested PCR combined with DNA probe test was excellent in terms of detection of mycobacterial organisms and identification of M. tuberculosis or MAC. The detection limits of the present method were 10 fg DNA for M. tuberculosis, and 100 fg DNA for MAC, respectively. We further investigated on the optimum temperature for hybridization in HPA with AE labeled DNA probe because there was the difference in the mode of DNA-RNA hybridization from that of DNA-DNA hybridization. In our method, the optimum temperature of hybridization was estimated as 55 +/- 1 degrees C. In preliminary experiments on two clinical cases, we practically detected and identified M. tuberculosis and MAC in clinical specimens, such as sputa, by using this newly devised method. We concluded that this method is useful for rapid detection and identification of M. tuberculosis and MAC in clinical specimens.

Adult↗

PubMed1994

Comparison of three advanced chromatographic techniques for cannabis identification.

The development of chromatography technology, with the increasing availability of easier-to-use mass spectrometers combined with gas chromatography (GC), the use of diode-array or programmable variable-wavelength ultraviolet absorption detectors in conjunction with high-performance liquid chromatography (HPLC), and the availability of scanners capable of reading thin-layer chromatography (TLC) plates in the ultraviolet and visible regions, has made for easier, quicker and more positive identification of cannabis samples that standard analytical laboratories are occasionally required to undertake in the effort to combat drug addiction. At laboratories that do not possess the technique of GC combined with mass spectrometry, which provides an irrefutable identification, the following procedure involving HPLC or TLC techniques may be used: identification of the chromatographic peaks corresponding to each of the three main cannabis constituents-cannabidiol (CBD), delta-9-tetrahydrocannabinol (delta-9-THC) and cannabinol (CBN)-by comparison with published data in conjunction with a specific absorption spectrum for each of those constituents obtained between 200 and 300 nm. The collection of the fractions corresponding to the three major cannabinoids at the HPLC system outlet and the cross-checking of their identity in the GC process with flame ionization detection can further corroborate the identification and minimize possible errors due to interference.

Bias↗

PubMed1993

DNA probes for the identification of malaria vectors.

The correct identification of the mosquito vectors of malaria is essential to determine the vectorial significance of the different species within species complexes and to optimise vector control programmes. A number of methods are available including cytogenetic analysis, isoenzyme characterisation, and mating incompatibility. We describe here the development of a simplified DNA probe methodology for the identification of malaria vector specimens, with particular emphasis on the identification of mosquitoes of the Anopheles gambiae complex, which includes the most important vectors of malaria in Africa. The method incorporates the use of a simple 'squash-blot' sample preparation, synthetic oligonucleotide probes, non-radioactive labelling and detection systems, cheap and stable reagents, and all processes are carried out at room temperature without the need for equipment. Identification of up to 300 specimens (larvae, pupae or adults) per day is possible by a single worker using this simple technique and costs $0.02-$0.04 per specimen.

Animals↗