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Antimicrobic effects of Lactobacillus fermentation on edible waste material contaminated with infected carcasses.

Survival of Newcastle disease virus (NDV) and Salmonella enteritidis serovar typhimurium (introduced as infected carcasses) in Lactobacillus-fermented edible waste material was studied to determine the ability of the fermentation to activate disease agents in carcasses. Two trials were conducted. In the 1st trial, the fermented wastes contained 20% infected carcasses (protein) consisting of equal numbers of chicken (NDV) and rat (Salmonella) carcasses, and the 2nd trial contained 40% carcasses (protein). Mixtures were incubated at 20 C, 30 C, and 40 C for 216 hours. Samples were obtained daily for quantitative virus and bacterial isolation. Temperature, pH, and redox potential were monitored. In both trials, pH and redox potential changes occurred between 24 and 48 hours depending on the incubation temperature. In both trials, NDV survived 4 days at 20 C, 2 days at 30 C, and 1 day at 40 C. Salmonella enteritidis serovar typhimurium survived 5 days at 20 C, 1 day at 30 C, and was not isolated from the 40 C samples after 24 hours in both trials.

Animal Feed↗

[Bacteriocinogenic typing of lactobacilli].

Sensitivity of 588 strains of Lactobacilli belonging to 15 species was studied with respect to 39 bacteriocinogenic strains of Lactobacilli producing 39 variants of bacteriocins. The study provided a set of 20 indicator strains of Lactobacilli. With the use of this set it is possible to mark Lactobacilli of various species and differentiate them into 39 bacteriocinogenic types. This may be used in taxonomic and epidemiological studies.

Bacteriocins↗

[Intestinal dysbacteriosis in yersiniosis patients and the possibility of its correction with biopreparations].

Intestinal dysbacteriosis was found in all 37 patients with intestinal yersiniosis or pseudotuberculosis. Administration of biopreparations containing lacto- or bifidobacteria produced a good clinico-microbiological response rate (above 90%). The authors think it valid to include biopreparations in combined treatment of yersiniosis to prevent unfavourable outcomes.

Acute Disease↗

[Electrophoretic characteristics of lactate dehydrogenase isoenzymes of lactobacilli and determination of their activity].

Lactate dehydrogenase isoenzymes of 10 museum strains of lactobacilli were examined electrophoretically in polyacrylamide gel. With respect to the relative electrophoretic motility and enzymic activity certain differences in the isoenzyme spectra of lactate dehydrogenase can give additional evidence for the identification of lactobacillar species.

Cell-Free System↗

[Ability of lactobacilli in the human microflora to produce lysozyme].

Lysozyme activity of 403 strains of lactobacilli were investigated; of these 26 were from foreign sets and 377 were isolated from the contents of the stomach, feces and vaginal discharge of healthy adults. The species reference of lactobacillae was confirmed by the results of study of their physiologo-biochemical properties with the aid of 45 tests. A method of agar plates was adapted to determination of lysozyme: the autoclaved suspension of Micrococcus lysodeikticus (strain No. 2665) was added to the agar medium MPC-I, and lactobacilli were cultivated for 4 days in the CO2 atmosphere at 37 degrees C. There was revealed the capacity of L. fermenti and L. brevis to produce lysozyme; in L. fermenti the lysozyme activity was much more frequent (p less than 0.001); strains of the rest of the species of lactobacilli differentiated by the Rogosa and Sharpe's classification proved to be lysozyme-negative. It was shown that the lactobacilli of the L. fermenti species, included into the microflora of the intestine and the vagina of healthy adults as a rule possessed lysozyme activity. L. fermenti strain 90T-S4 used in the production of dry lactobacterin also produced lysozyme. All this favours an important role of L. fermenti in the protective function of the microflora.

Adult↗

[Wound infection in the surgical treatment of children with congenital cleft palate].

Clinico-microbiologic examinations of 153 children with cleft palate aged 2 to 9 were carried out. Two groups of patients with different pattern of operative wound healing after uranoplasty were singled out: 110 ones with primary healing and 13 ones with infectious inflammations of the wound. The incidence of S. aureus carriership on the palatal mucosa before surgery was higher among patients who developed postoperative inflammations. On day 3 after the operation gram-negative bacilli were isolated from the majority of patients with postoperative wound inflammations coursing in the presence of marked dysbacteriosis as against patients in whom the wounds healed by primary intention. The authors suggest a method for wound infection prevention in uranoplasty, consisting in irrigation of the operative wound with acilact (a biopreparation) suspension and shortening of antibiotic prevention course to just 48-72 h; the efficacy of this method was confirmed by a reduction of the incidence of postoperative complications. Normal microflora representatives predominated in the microbiocenosis of the operative wound on days 3 and 10-12 after uranoplasty.

Anti-Bacterial Agents↗