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At least 163 records · Page 9Linked to original sources

PubMed2005

Clinical evaluation of packable and conventional hybrid posterior resin-based composites: results at 3.5 years.

BACKGROUND: The authors evaluated clinical performances of a packable and a conventional hybrid resin-based composite used with a self-etch adhesive system. METHODS: Three dentists placed 105 posterior restorations in 65 adults. They placed a packable (SureFil, Dentsply DeTrey GmbH, Konstanz, Germany) and a conventional (Spectrum, Dentsply DeTrey GmbH) resin-based composite using a self-etch resin adhesive system. The authors evaluated the restorations using Ryge modified criteria, photographs and die stone replicas. RESULTS: After 3.5 years, six large SureFil and two Spectrum restorations had failed from bulk fracture and secondary caries, resulting in cumulative survival rates of 81.3 and 92.0 percent, respectively. Failed SureFil restorations generally were larger than the remaining intact restorations. Other ratings were satisfactory, with no significant differences between the two materials for any restoration parameter. Alfa ratings for both materials were approximately 80 percent or greater for marginal discoloration, anatomical form, surface texture and surface staining. Lower percentages of restorations were rated Alfa for color match, marginal integrity and gingival health. No postoperative sensitivity was reported. Net mean occlusal wear (+/- standard deviation) was 28.9 (+/- 32.9) micrometers for SureFil and 33.8 (+/- 29.6) microm for Spectrum restorations; the difference was not statistically significant. CONCLUSIONS: When used with a self-etch adhesive, the 3.5-year clinical performances of both composites were similar and satisfactory for the restoration of Class I and moderate-sized Class II cavities. Clinical Implications. The two composites placed in this study have an increased risk of bulk fracture when placed in large intracoronal Class II molar preparations.

Acid Etching, Dental↗

PubMed1965

Agreement Between Deoxyribonucleic Acid Base Composition and Taxometric Classification of Gram-Positive Cocci.

Silvestri, L. G. (Università Statale, Milan, Italy), and L. R. Hill. Agreement between deoxyribonucleic acid base composition and taxometric classification of gram-positive cocci. J. Bacteriol. 90:136-140. 1965.-It had been previously proposed, from taxometric analyses, that gram-positive, catalase-positive cocci be divided into two subgroups. Thirteen strains, representative of both subgroups, were examined for deoxyribonucleic acid (DNA) base composition, determined from melting temperatures. Per cent GC (guanine + cytosine/total bases) values fell into two groups: 30.8 to 36.5% GC and 69 to 75% GC. Strains with low per cent GC values belonged to the Staphylococcus aureus-S. saprophyticus-S. lactis taxometric subgroups, and those with high per cent GC values belonged to the S. roseus-S. afermentans subgroup. The hypothetical nature of any classification is emphasized, and, in the present work, the hypothesis derived from taxometric analyses of division into two subgroups is confirmed by the study of DNA base ratios. The two subgroups correspond, respectively, to the genera Staphylococcus and Micrococcus.

Journal Article↗

PubMed2004

Comprehensive analysis of the base composition around the transcription start site in Metazoa.

BACKGROUND: The transcription start site of a metazoan gene remains poorly understood, mostly because there is no clear signal present in all genes. Now that several sequenced metazoan genomes have been annotated, we have been able to compare the base composition around the transcription start site for all annotated genes across multiple genomes. RESULTS: The most prominent feature in the base compositions is a significant local variation in G+C content over a large region around the transcription start site. The change is present in all animal phyla but the extent of variation is different between distinct classes of vertebrates, and the shape of the variation is completely different between vertebrates and arthropods. Furthermore, the height of the variation correlates with CpG frequencies in vertebrates but not in invertebrates and it also correlates with gene expression, especially in mammals. We also detect GC and AT skews in all clades (where %G is not equal to %C or %A is not equal to %T respectively) but these occur in a more confined region around the transcription start site and in the coding region. CONCLUSIONS: The dramatic changes in nucleotide composition in humans are a consequence of CpG nucleotide frequencies and of gene expression, the changes in Fugu could point to primordial CpG islands, and the changes in the fly are of a totally different kind and unrelated to dinucleotide frequencies.

AT Rich Sequence↗

PubMed2003

Polymerization contraction stresses of resin-based composite restorations within beveled cavity preparations of Class I restorations.

PURPOSE: To evaluate internal stresses resulting from polymerization of resin-based composite materials in cavity preparations beveled at the internal line angles or the cavosurface margin, compared with those in conventional butt-joint box-shaped cavity preparations. MATERIALS AND METHODS: Contraction stress generated in resin-based composite restorations placed in an experimental cavity preparation model was determined using micro-photoelastic analysis. Three types of cavity preparations simulating Class I restorations with bevels at internal line angles (1 mm and 2 mm deep), and at the cavosurface margin (2 mm deep) were prepared in a posterior composite model in order to obtain no marginal defect with the restorative composite. The transparent restorative composite was bulk-filled into the preparations and light-cured for 80 seconds. Specimens 2 mm thick for micro-photoelastic analysis were cut perpendicular to the long axis of the preparation. Fringe patterns for directions and magnitudes of stresses were obtained using transmitted and reflected polarized light. Then, the photoelastic stress analysis was performed to examine stresses in the preparations. The data were statistically compared with the previous data in conventional butt-joint box-shaped preparations by ANOVA and Scheffé's F test (P < 0.05). RESULTS: In 1 and 2 mm deep internal beveled cavity preparations, the maximum principal stress lines were parallel to the wall at the straight internal bevels. In the preparation with a round bevel at the cavosurface margin, the stress distribution in the bulk of the preparation was similar to that in the butt-joint preparation. Maximum stress values in 1 and 2 mm deep internal beveled preparations were 12.2 +/- 0.2 MPa and 18.8 +/- 2.8 MPa, respectively. For 2 mm-deep preparation, the maximum stress values in the internal beveled preparations were significantly lower than those values in butt-joint box-shaped cavity preparations. Maximum stress values in the preparations with the bevel at the cavosurface margin were 22.7 +/- 1.0 MPa, and were not significantly different from those values observed in the butt-joint preparations.

Analysis of Variance↗

PubMed2001

Clinical evaluation of replacement of class V resin based composite restorations.

OBJECTIVES: The purpose of this clinical study was to evaluate any increase in size of Class V resin based composite restorations that required replacement. Emphasis was placed on measurements of the loss of tooth structure in areas distant from the site of failure. METHODS: 14 Class V restorations scheduled for replacement were recorded with pre-operative impressions and intra-oral photographs. Restorations were then removed and cavity preparations were completed. A second impression and photograph were taken and stone casts were prepared for each impression. The perimeter and area of each restoration and cavity preparation were measured with a video-based imaging system (Dage 725 CCD camera) equipped with a macro lens, at a fixed magnification of x 10-15. The initial measurements of the restoration image were compared to the measurements of the new cavity preparation, in both instances excluding the site of failure. Intra-class correlation coefficient (ICR) and paired t-test were used for statistical analysis. RESULTS: A significant difference between the pre and post-operative measurements for both area (P=0.0125) and perimeter (P=0.003) was found. CONCLUSION: The study showed that replacement of Class V resin based composite restorations resulted in increased size of the cavity preparation in areas distant from the site of failure.

Composite Resins↗

PubMedDate not supplied

Preparation of uniform titanium dioxide (TiO2) polystyrene-based composite particles using the glass membrane emulsification process with a subsequent suspension polymerization.

Uniform titanium dioxide (TiO(2))-polystyrene-based composite particles were prepared using the glass membrane emulsification process followed by a subsequent suspension polymerization. The oil phase, consisting of anatase TiO(2) fine powder, monomers, methyl laurate as the hydrophobic additive, Disperbyk-180 and the poly(styrene-co-2-ethyl hexylacrylate) were emulsified through the membrane pores into the aqueous phase containing stabilizers to form a (solid-in-oil)-in-water (S/O/W) emulsion of monomer droplets. The suspension polymerization was carried out at 343 K for 24 h under a nitrogen atmosphere. An SPG membrane with a pore size of 5.25 microm was employed and 20-25 microm TiO(2)-polystyrene based composite particles were obtained depending on the composition of polymerizing oil phase. The effects of the co-monomer, 2-ethylhexyl acrylate and the cross-linking agent, divinyl benzene on the dispersion stability of TiO(2) in the oil phase, the surface feature of the particle and the encapsulation loading were investigated in this study. The membrane emulsification process was capable of preparing the composite particles with approximately 5 wt% of TiO(2) encapsulated, which accounts for with at least 85 wt% of TiO(2) in the oil phase.

Drug Compounding↗

PubMed2002

Energy dependent polymerization of resin-based composite.

OBJECTIVE: This study explores the relationship between the extent of polymerization and the radiant energy (dose) applied during the photopolymerization of resin-based composites. METHOD: FTIR was used to measure the 5-min and 24-h conversion of four resin-based composites prepared in a thin film and polymerized under conditions of decreasing intensity and a constant exposure time (30s) using a tungsten halogen curing light. The measured conversion was obtained over a wide range of applied radiant energy. Additionally, samples for two of the materials were polymerized at various intensities and exposure times such that the dose remained constant. This process was performed at four dose levels representing approximately 75% of the conversion range. RESULTS: The curing profiles (percent conversion versus applied radiant energy) depict a gradual decrease in conversion with decreasing energy followed by a rapid descent. Though there are differences in the maximum conversion attained between the materials, when conversion is represented as a fractional conversion relative to the maximum 24-h value, their 5-min and 24-h curing profiles appear quite similar. Additionally, very similar conversion was measured when the films were exposed using equivalent doses providing evidence for a reciprocal relationship between irradiance (power density) and exposure time. For the 24-h measurements, statistical equivalence (Fishers protected LSD at the 0.05 level) was noted for most of the combinations of exposure time and power density within a given dose. Generally, the exceptions occurred with the shortest exposure times. SIGNIFICANCE: A reciprocal relationship between exposure time and power density adds significance to the study of conversion as a function of the total applied dose. This relationship establishes the curing profile as a universal correlation between exposure time and power density.

Acrylic Resins↗

PubMed2004

Effect of chemical structure and composition of the resin phase on mechanical strength and vinyl conversion of amorphous calcium phosphate-based composites.

The mechanical properties of recently developed bioactive, antidemineralizing/remineralizing, amorphous calcium phosphate (ACP)-based composites need improvement. The objective of this study was to elucidate the effect of structure and composition of resins on the biaxial flexure strength (BFS) and the degree of conversion (DC) of composites attained after photo-polymerization. Two series of 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenyl]propane (Bis-GMA)/triethylene glycol dimethacrylate (TEGDMA)/X (X being a neutral or acidic comonomer) ternary resins were prepared and mixed with a mass fraction of 40% of zirconia-hybridized ACP. Both unfilled copolymers and their composites were evaluated for BFS (dry and wet specimens after 2 weeks of immersion in buffered saline) and for DC (after 24 h at 23 degrees C). It was found that for the neutral X monomers, no correlation existed between the hydrophobic/hydrophilic character of the X monomer and the BFS values of the immersed composites. A flexible monomethacrylate yielded copolymers and composites with the highest DC. For the resins utilizing the acidic comonomers, methacrylic acid and mono-4-(methacryloyloxy) ethyltrimellitate (4MET), dry composites with improved BFS values were obtained. 4MET composites exhibited the least loss of strength of all the ternary resin ACP materials. The effect of acidic X on DC was most pronounced for maleic acid copolymers.

Biocompatible Materials↗

PubMed1977

Base composition of DNA from some reptiles.

The DNA base composition was studied in 9 reptilian species (4 chelonians, 3 saurians, and 2 ophidians) by the thermal denaturation method. This preliminary investigation has brought to light differences in GC percentages and in the shape of melting curves between chelonian DNA and that from saurians and ophidians.

Animals↗

PubMed1968

Deoxyribonucleic acid base composition of the genus Lactobacillus.

Deoxyribonucleic acids of 45 strains of Lactobacillus and 5 strains of Bifidobacterium which had been analyzed for base composition by chromatographic means were examined at equilibrium in a CsCl density gradient. Regression analysis showed that there have been systematic errors involved in the estimation of guanine plus cytosine (GC) content by the chemical method, and that the relation between buoyant density and base composition is indeed linear and best fitted by the equation GC = 10.309 (rho-1.662), which compares well in slope with the equation of Schildkraut, Marmur, and Doty. With the improved data obtained in this study, the specific groupings of the species of both genera were reevaluated.

Centrifugation, Density Gradient↗

PubMed2005

3-D molecular assembly of function in titania-based composite material systems.

Various examples of composite titania-based nanostructured materials exhibiting cooperative functionalities between different active components are presented. The fabrication of these integrated composite materials is based on one-pot supramolecular templating techniques combined with acidic sol-gel chemistry. The defined 3-D nanoscale organization and integration of various functional components results in advanced optoelectronic and photonic applications such as visible light sensitization of mesoporous titania photocatalysts with cadmium sulfide nanocrystals acting as sensitizing integral part of the mesopore wall structure, narrow bandwidth emission from rare earth ion activated nanocrystalline mesoporous titania films, and mirrorless lasing in dye-doped hybrid organic/inorganic mesostructured titania waveguides.

Journal Article↗

PubMed1994

Influence of nucleic acid base composition on radiation-induced strand breakage in single stranded DNA: a time resolved study.

The following study investigates the pathways involved in the induction of single strand breaks (ssb) in various samples of single stranded (ss) DNA (calf thymus, Micrococcus lysodeikticus, Clostridium perfringens) with differing nucleic acid base composition. The time scale for the induction of ssb was determined from changes in the light scattering intensity following pulse irradiation of aqueous solutions containing these ssDNA samples at pH7.8 under either aerated or deaerated conditions. The induction of ssb under these conditions is predominantly by the hydroxyl radical and shows various kinetically distinct components. The immediate ssb (t < 0.02 s) account for approximately 40-60% of the total yield of ssb. The majority of these ssb are suggested to arise from the 'common' initial attack of the hydroxyl radicals at the sugar phosphate backbone for each of the three DNA samples. Furthermore, slower components for ssb formation (t > 0.02 s) were observed and are suggested to occur through base radical mediated H-atom abstraction from the sugar moiety. The half lives for formation of the majority of ssb, formed through this base radical-mediated H-atom abstraction(s), are in the range of 20-43 ms. The yields of these 'base-mediated' ssb vary markedly (under both aerobic and anaerobic conditions) and reflect the base composition of the DNA sample. It is suggested from these studies that the OH-induced base radicals of guanine/cytosine are more effective precursors for strand breakage than those from adenine/thymine in ssDNA.

Base Composition↗

PubMed2001

Four-year clinical evaluation of posterior resin-based composite restorations placed using the total-etch technique.

PURPOSE: To report the 4-year clinical performance of posterior resin-based composite restorations placed using the total-etch technique. MATERIALS AND METHODS: Over a period of 1 year, 726 restorations (248 molars, 478 premolars; 260 Class I, 466 Class II; 540 replacements, 186 primary decay) were placed on conservative preparations, using the incremental placement technique in a clinical environment. Baseline data were collected, and the restorations were evaluated after 4 years. Z100 and Scotchbond Multi-Purpose (SBMP) (3M Dental Products, St. Paul, Minnesota) were used as the restorative system. The criteria evaluated were color match, marginal adaptation, anatomic form, cavosurface marginal discoloration, axial contour, interproximal contact, secondary caries, postoperative sensitivity, and tooth vitality. RESULTS: At baseline, 24% of the teeth restored presented postoperative sensitivity; 86% of the sensitive teeth were from the replacement group. At 4 years, all teeth were vital to cold test. Eighteen restorations (2.5%) presented clinically detectable marginal fracture. The shade was acceptable in all restorations. Cavosurface marginal discoloration was observed in 47 restorations (6.5% bravo scores). Axial contour, interproximal contact, and marginal adaptation received 100% alfa scores. No secondary caries was diagnosed in any of the teeth examined. None of the examined restorations required replacement. CLINICAL SIGNIFICANCE: Under controlled clinical conditions, posterior resin-based composite restorations placed with the total-etch technique and restorative Z100/SBMP have the potential to present a high success rate at 4 years. None of the examined restorations required replacement, and there was no clinically detectable wear in any of the restorations. Simultaneous etching of enamel and dentin followed by the application of a resin adhesive can be considered an adequate modality of pulp protection in nonexposed tooth preparations.

Acid Etching, Dental↗

PubMed1999

Additional support for Afrotheria and Paenungulata, the performance of mitochondrial versus nuclear genes, and the impact of data partitions with heterogeneous base composition.

We concatenated sequences for four mitochondrial genes (12S rRNA, tRNA valine, 16S rRNA, cytochrome b) and four nuclear genes [aquaporin, alpha 2B adrenergic receptor (A2AB), interphotoreceptor retinoid-binding protein (IRBP), von Willebrand factor (vWF)] into a multigene data set representing 11 eutherian orders (Artiodactyla, Hyracoidea, Insectivora, Lagomorpha, Macroscelidea, Perissodactyla, Primates, Proboscidea, Rodentia, Sirenia, Tubulidentata). Within this data set, we recognized nine mitochondrial partitions (both stems and loops, for each of 12S rRNA, tRNA valine, and 16S rRNA; and first, second, and third codon positions of cytochrome b) and 12 nuclear partitions (first, second, and third codon positions, respectively, of each of the four nuclear genes). Four of the 21 partitions (third positions of cytochrome b, A2AB, IRBP, and vWF) showed significant heterogeneity in base composition across taxa. Phylogenetic analyses (parsimony, minimum evolution, maximum likelihood) based on sequences for all 21 partitions provide 99-100% bootstrap support for Afrotheria and Paenungulata. With the elimination of the four partitions exhibiting heterogeneity in base composition, there is also high bootstrap support (89-100%) for cow + horse. Statistical tests reject Altungulata, Anagalida, and Ungulata. Data set heterogeneity between mitochondrial and nuclear genes is most evident when all partitions are included in the phylogenetic analyses. Mitochondrial-gene trees associate cow with horse, whereas nuclear-gene trees associate cow with hedgehog and these two with horse. However, after eliminating third positions of A2AB, IRBP, and vWF, nuclear data agree with mitochondrial data in supporting cow + horse. Nuclear genes provide stronger support for both Afrotheria and Paenungulata. Removal of third positions of cytochrome b results in improved performance for the mitochondrial genes in recovering these clades.

Animals↗

PubMed1996

Cytophotometric determination of heterochromatin base composition in interphase nuclei of plant cells.

The use of DNA base-specific fluorochromes and static cytophotometry at different thresholds of fluorescence intensity is proposed for studying the base composition of differently condensed chromatin fractions in plant interphase nuclei. Results are reported of such analyses on nuclei from leaflets of Posidonia oceanica and Helianthus annuus, which confirm other findings obtained on the same materials by biochemical and molecular methods.

Base Composition↗

PubMedDate not supplied

Teaching of posterior resin-based composite restorations in Brazilian dental schools.

OBJECTIVE: The purpose of this study was to evaluate the teaching program of Class I and Class II resin-based composite (RBC) restorations in Brazilian dental schools and to observe if any differences were found from similar surveys conducted in North American, European, and Japanese dental schools. METHOD AND MATERIALS: A questionnaire containing 15 questions was distributed to 92 Brazilian dental schools, and 64 (70%) schools returned the questionnaire. The questions inquired the amount of time the curriculum dedicated to teaching of posterior RBC restorations, future expectation regarding the teaching time, limitation in extension of the occlusal width and the proximal box in Class II, contraindications for placing posterior RBC restorations, protocol for using bases and liners, brand of bonding agents and RBC used, instruments and techniques employed for finishing, cost relative to amalgam restorations, and biologic reactions related to the use of posterior RBC. The responses were calculated as percentages based on the number of schools that responded to the questionnaire. Where appropriate, the Chi-squared test and the Fisher exact test were used for statistical analysis. RESULTS: Of the dental schools that responded, 88% dedicated 10% to 50% of the teaching time in operative dentistry to posterior RBC restorations. A significant correlation (P = 0.041) was found between the percentage of time dedicated to the teaching of posterior RBC restorations and the higher cost of posterior RBC compared to amalgam restorations. Resin-based composite restorations cost 30% to 70% more than amalgam restorations in the 40% of dental schools that charged a fee. Posterior composites for large restorations in molars were used by 14% of the dental schools. Base and liner were not placed by 10% of dental schools in deep Class I or Class II RBC restorations. One school did not recommend acid etching of the dentin. CONCLUSION: No major differences were found in the teaching philosophy of posterior RBC restorations by comparing the Brazilian data to the data from similar surveys done in North America, Japan, and Europe.

Acid Etching, Dental↗

PubMed2004

Influence of adhesive system and placement technique on microleakage of resin-based composite restorations.

PURPOSE: To analyze the influence of two placement techniques and two adhesive systems on the marginal microleakage of Class II resin-based composite restorations using different resin composite materials. MATERIALS AND METHODS: Two hundred standardized Class II cavities (5 mm x 3 mm x 1.5 mm) were cut in human premolars and restored using five resin composite materials (A.L.E.R.T.-AL, Solitaire-SO, SureFil-SU, Filtek A110-A, and Z100-Z), two adhesive systems (Bond 1-B1 and Etch & Prime 3.0-EP), and two placement techniques (incremental or bulk). Specimens were thermocycled (700 cycles, 5 degrees - 55 degrees C, 1 min dwell time), immersed in 0.5% methylene blue solution for 4 h, and sectioned longitudinally to evaluate the extent of microleakage. Data were submitted to statistical analysis using the Kruskal-Wallis test. RESULTS: It was observed that both adhesive systems presented similar results (p > 0.05), and neither achieved complete marginal sealing. The bulk placement technique led to greater microleakage than the incremental technique (p < 0.001). Restorations prepared using SO resin composite showed greater microleakage than those performed using Z or AL materials. However, SO obtained similar values of microleakage when compared to SU and A materials. CONCLUSION: The use of incremental placement technique resulted in less microleakage for Class II resin-based composite restorations. No difference was observed among adhesive systems regarding prevention of marginal dye penetration. Furthermore, the type of resin composite material employed for restoration may have an influence on the extent of microleakage.

Bicuspid↗

PubMed1990

Dependence of the torsional rigidity of DNA on base composition.

The Escherichia coli phage 434 repressor binds as a dimer to the operator of the DNA helix. Although the centre of the operator is not in contact with protein, the repressor binding affinity can be reduced at least 50-fold by changing the sequence there: operators with A.T base pairs near their centre bind the repressor more strongly than do operators with G.C base pairs at the same positions. To explain these observations, it has been proposed that the base composition at the centre of the operator affects the affinity of the operator for repressor by altering the ease with which operator DNA can undergo the torsional deformation necessary for complex formation. In this model, the variation in binding affinity would require the torsion constant to have specific values and to change in a sequence-dependent manner. We have now measured torsion constants for DNAs with widely different base compositions. Our results indicate that the torsion constants depend only slightly on the overall composition, and firmly delimit the range of values for each. Even the upper-limit values are much too small to account for the observed changes in affinity of the 434 repressor. These results rule out simple models that rely on substantial generic differences in torsion constant between A.T-rich sequences and G.C-rich sequences, although they do not rule out the possibility of particular sequences having abnormal torsion constants.

Base Composition↗