SEARCH · PubMed Health

Results for “Basophil Degranulation Test”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 163 records · Page 9Linked to original sources

PubMed2001

Drug allergy diagnosis in humans: possibilities and pitfalls.

Due to the potential hazards of drug allergies, an early and reliable diagnosis is crucial. The use of in vivo tests is not recent but, because of the hazards of skin testing in patients with a history of anaphylaxis, they had been abandoned for a while. Recent reevaluations have shown that for some drugs, e.g. antibiotics-reliable skin tests can ensure the diagnosis of drug allergy in up to 70% of cases. Many in vitro tests based on well-defined mechanisms, e.g. the basophil degranulation test have been used for the diagnosis of totally unrelated allergic mechanisms. It is almost impossible to interpret their validity as diagnosis tools. Nevertheless, other tests, e.g. the lymphocyte transformation test which have been evaluated in well-conducted recent studies, seem to have a good predictive value. Their use is still restricted to clinical trials or research studies. A reliable clinical approach as well as a detailed examination of the drug intake remains obligatory to diagnose drug allergy. Available in vivo and in vitro tests are sometimes used to confirm the diagnosis. The sensitivity and specificity of these tests is evaluated in clinical studies. Research to improve the existing tests and to develop new diagnostic tools is still of paramount importance.

Animals↗

PubMed1988

[Leukocyte histamine liberation tests for the diagnosis of anaphylactic reaction to curare-like agents].

Anaphylaxis to myorelaxants is the main etiology of adverse reactions to anesthetics and adjuvants. The diagnosis relies on skin tests--prick or intradermal tests--radio-immunoassays for series specific IgE, human basophil degranulation tests and leucocyte histamine++ release. 28 patients with an anaphylaxis to myorelaxants have been studied, using the two tests. Skin tests are the more specific and accurate ones. ARIA using a quaternary ammonium hydrated gel, is highly specific and its sensitivity reaches 80%. The leucocyte histamine release may be interest in evaluating anaphylaxis especially when another drug might be involved. The sensitivity of HBDT is low, indicating that HBDT is to be discarded as far as other tests have been developed, for myorelaxants. However, its usefulness for other drugs has to be compared to leucocyte histamine release.

Anaphylaxis↗

PubMed1984

[Cellular immunology tests applied to the diagnosis of immune system dysfunctions].

In clinical immunology, improved standardisation and simplification have made skin and laboratory tests more amenable both to routine use and more rigorous interpretation. An individual's immune status can be assessed by the in vivo investigation of delayed hypersensitivity to widely distributed antigens or by techniques such as the mitogen test ot lymphoblast transformation, lymphocyte population and sub-population (e.g. T4, T8...) counts. A more delicate test of phagocytosis is provided by the chemoluminescence test. Investigation of allergic states (immediate hypersensitivity) relies on skin tests and also on laboratory techniques such as the basophil degranulation test.

Blood Bactericidal Activity↗

PubMed1987

Standardization of basophil degranulation for pharmacological studies.

There is a need of a standard basophil degranulation test for diagnosis and for immunopharmacological studies. In order to obtain a sufficient concentration of basophils blood was sedimented at 1 g which gave a yield of 1500-3000 basophils/microliter instead of 50/microliter in whole blood. As the course of degranulation is exponential, it can be linearised using a logarithmic approximation Y = A0 + A1 log D where D is the allergen concentration. The application of this mathematical method permitted the collection of statistical information about the test itself. The coefficient of variation was less than 10%. The test can be used to compare the allergenic potency of different extracts of the same allergen.

Basophils↗

PubMed2006

Comparision of two-flow cytometry methods for basophil degranulation in patients sensitized to grass pollen.

BACKGROUND: Two-flow cytometry methods for quantification of degranulated basophil after allergen-specific activation were discussed. The methods are discerned by used membrane receptors--FcepsilonRI or IL-3Ralpha Our goal was to evaluate the diagnostic potential of the methods and to correlate them to allergen-specific IgE detection and skin prick test (SPT). METHODS: Patient's and control's groups were studied with Bulgarian grass pollen allergen B1 simultaneously by flow cytometry kits: Basotest and BD FastImmune test. Allergy diagnosis was based on clinical history and SPT. The determination of specific IgE was performed by ELISA--RIDASCREEN. RESULTS: There were no significant differences between the patient's results from Basotest and FastImmune (P>0.05). A significant correlation between values, analyzed by Basotest and by FastImmune was found (r=0.88). The sensitivity and specificity of the Basotest, FastImmune, specific IgE and SPT were 85%, 72%, 92% and 92% sensitivity and 100%, 92%, 100% and 85% specificity respectively. The efficiency was between 82% and 97%. There were a significant correlation between the specific IgE and flow cytometry tests: tau=0.92 (Basotest) and tau=0.71 (FastImmune) and a moderate significant correlation between the SPT and the in vitro tests: tau=0.26 (Basotest) and tau=0.31 (FastImmune). CONCLUSION: The successful use of the Bulgarian grass pollen allergen B1 and both flow cytometry tests was presented. These methods could be as specific tools for IgE-mediated diagnosis especially FastImmune in the case of low IgE receptor expression.

Adolescent↗

PubMed1982

[Immediate hypersensitivity in brucellosis. New data (author's transl)].

The presence of clinical manifestations of the "immediate hypersensitivity" process has been frequently described in veterinarians, bacteriological laboratory technicians and some patients with chronic brucellosis. We have looked for the presence of specific IgE in these conditions. Twenty-four patients, three months at least after the acute phase of their brucellosis, were given exploration of their humoral, cellular and specific immunity against brucellosis. For this purpose, beside usual classical tests: serodiagnosis, total and specific IgE assays, intradermoreaction and lymphoblastic transformation test with specific antigen (PI fraction) and an adaptation of human basophil degranulation test (HBDT) with PI fraction were performed on all 24 patients. Exploration of immediate hypersensitivity allows us to show neither an abnormal increase of hyper-IgE frequency nor an increase of anti-brucella specific IgE. HBDT is positive in 41 p. cent of the patients. It confirms that immediate hypersensitivity exists. HBDT gives justification for trying desensitization which has been proposed in the past. HBDT will possibly allow us to evaluate desensitization effectiveness.

Acute Disease↗

PubMedDate not supplied

Fixed drug eruption.

We presented 21 patients with fixed drug eruptions. All of them had positive provocation tests, showing the usefulness of this test for diagnosis. The lesions appeared on the skin or mucous membranes within 20 min. to 10 hours, after an oral provocation test with a sub-therapeutic dose. In those cases where we performed the basophil degranulation test, they were all negative. The drug more frequently implicated was cotrimoxazole (71%). In one patient the eruption was produced by oral neomycin.

Administration, Oral↗

PubMed1993

Responsiveness of basophil granulocytes of horses suffering from chronic obstructive pulmonary disease to various allergens.

As basophils are the major effector cells of allergic reactions, confirmation of the allergic etiology of chronic obstructive pulmonary disease (COPD) was sought by the demonstration of a specific in vitro response of equine basophilic blood cells to some potential allergens (Aspergillus, Cladosporidium, Mucor, Penicillium, extracts of dust particles of hay and straw). The allergen induced degranulation of basophils and the histamine and protease release from basophils during incubation with the allergens were tested. By evaluating the results obtained from 14 COPD horses and eight controls it could be shown that the sensitivity of the basophils of affected horses was increased, particularly against the allergen extract of Mucor mucedo and Mucor spinosus. Further a greater percentage of COPD horses reacted positively with the Mucor allergen extract. The mitogenic stimulation of lymphocytes by PHA and by the allergen extracts used gave comparable results in affected and control horses. Thus the in vitro stimulation of basophils may be an easily to perform testing device for the identification of potential allergens involved in the pathogenesis of equine COPD.

Allergens↗

PubMedDate not supplied

[Evaluation of the activities and value of allergo-anesthetic consultation at the University Hospital Center of Nice 1985-1991].

Life-threatening accident during anesthesia scarcely happen but the consequences may be dramatic. We report our experience of an allergo-anesthesia consultation created since 1985 in Nice hospital. 452 patients have been investigated: 1) 109 for life-threatening anaphylactic and anaphylactoid drug reactions. They have been investigated by: skin tests intradermal reactions (IDR) and prick tests with substances used during anesthesia (drugs and latex) and for all the muscle relaxants; the radioabsorbent test (RAST) for the muscles relaxants, propofol and latex; the human basophilic degranulation test (HBDT) for all the other drugs. We used the imputability decision table to classify the reactions. When anaphylaxis diagnosis was established (14) an "allergy card" was given to patients which identified the drugs to which they had a positive reaction. 62 patients have presented an anaphylaxis: 57 due to muscle relaxants (37 due to suxamethonium), 4 to latex and 1 to a gelatin. Patients were subsequently contacted and 50 of the 58 have responded. 18 of these patients have received 22 new anesthesias. Without exception, the advises to avoid a drug have been followed, 17 patients have a positive reaction to a muscle relaxant. In four of these, another muscle relaxant (skin test negative) was used without any trouble. For the other 13 who had shown a cross reactivity, all the muscle relaxants had been rejected and another anesthetic technique have been used: local anesthesia (3 cases), epidural (2 cases) associated or not with narcotics (propofol, midazolam), general anesthesia (propofol, midazolam, droperidol, phenoperidine). These drugs were all skin test negative.(ABSTRACT TRUNCATED AT 250 WORDS)

Anaphylaxis↗

PubMed1982

[Nephrotic syndrome and hypersensitivity to pollens (author's transl)].

In a 23-year-old man with recurrent nephrotic syndrome the responsibility of pollens from graminaceous plants could be demonstrated on clinical grounds (seasonal character of the syndrome, onset of proteinuria 24 hours after injection of the allergens) and by laboratory tests (increase in IgE levels, presence of specific IgE's, positive basophil degranulation test). There is ample evidence that some cases of nephrotic syndrome may be of allergic origin. A systematic search for air and food allergens should be considered, since the discovery of these allergens may lead to an aetiological treatment.

Adult↗

PubMedDate not supplied

Polymyxin B-induced basophil degranulation in atopic and non-atopic asthmatic patients.

It is suggested that increased reactivity in asthma is not limited to the bronchi but also exists in the mediator releasing system. We have previously observed that the basophils of atopic asthmatics are degranulated by polymyxin B in vitro. The present study attempts to relate this basophil degranulation to atopy. The basophil degranulation test in polymyxin B was performed in 28 atopic and 23 non-atopic asthmatic patients and 10 healthy controls. In 20 pollen-sensitive asthmatics, the test was performed during the pollen season and repeated when the season was over. In patients the total level of IgE was measured by enzyme immunoassay. Basophil degranulation with polymyxin B was observed in 18 of 28 atopics and in 3 of 23 non-atopic patients. There were no differences in the magnitude of degranulation between the pollen season and when the season was over. The level of IgE did not correlate with the magnitude of basophil degranulation (correlation index = 0,23).

Asthma↗

PubMed1982

[Value of basophil studies in the diagnosis of drug allergies].

Mastocytes and basophils are the two cell populations involved in the reactions seen during drug allergies. The testing of basophils is the only and simplest way of studying sensitization in the IgE system by a drug antigen, especially when the skin tests cannot be carried out. This study is carried out in two steps: straight after the drug-induced accident, where a basophil count will give an idea of its allergic origin; and some time after the accident, where the human basophil degranulation test (H.B.D.T.) helps to identify in over 50 p. 100 of cases the responsible drug. The basophil count and H.B.D.T. techniques are then described. The authors present their own results. In a study by H.B.D.T. of 908 patients with a drug allergy, 53.2 p. 100 had positive test; 57.6 p. 100 of 33 cases who presented an anaesthetic accident had a positive H.B.D.T.

Anesthetics↗

PubMed1990

Allergy testing using degranulation of basophils and flow cytometry.

The Technicon H6000 hematology system was evaluated as a detector for the allergen-induced basophil degranulation reaction in whole blood samples from individuals with type I allergy. Diluted standard skin prick test allergen extracts were added into heparin-EDTA blood samples in presence and absence of free calcium ions and the H6000 basophil channel scattergram was used with a modification of the method for basophil counting. In one experiment only 250 microliters of blood was used for each allergen to be tested. The result correlated well to skin prick test and RAST results. The conclusions are that the H6000 is suitable for allergy testing using whole blood from patients, if the basophil channel is modified, and that the Technicon H1 system probably also is possible to use as a detector for basophil degranulation studies.

Allergens↗

PubMed1991

[Diagnosis of allergic rhinitis. I. Anamnesis--ENT medical examination--skin tests--intranasal provocation].

One of the most important factors in the diagnosis of allergic diseases of the nose is the history, which requires a detailed knowledge of the allergic disease, and of the personal and occupational environment of the patient. Skin tests usually confirm the allergens suggested by the history. If this is not the case, a further history must be taken with respect to the agents identified by the positive skin tests. Other additional investigations must be performed such as RAST, the human basophil degranulation test, the histamine liberation test and the intranasal provocation test (IPT) with anterior rhinomanometry. The guidelines of the working group on bronchial and nasal provocation tests of the German Society for Research into Allergy and Immunity for the performance of the IPT should be respected because they are reproducible and standardised.

Desensitization, Immunologic↗

PubMed1987

[Occurrence of IgE-dependent food allergy in asthmatic disease].

The incidence of food allergy has been evaluated in 67 asthmatic patients (allergic asthma: 52, intrinsic asthma: 15). History was positive for post-meals attacks (7), symptoms involving other target organs (61%). Prick tests were found positive in 17/60; intradermal 77/682. Out of 110 human basophil degranulation tests or RAST, performed in 44 patients, 21/15 patients were found positive. Out of 18 inhalation challenges, 7 were positive. Out of 17 food oral challenges, 6 were positive (8 patients). A definite diagnosis of food allergy was accepted for 9 patients. Elimination diet, with 600 mg/day of cromolyn, induced a major improvement in 7 patients within 3 months, with 2 further failures after 1 year. A successful elimination diet was correlated with a positive food challenge. The relative incidence of food allergy in asthmatic adults is discussed (5.8%), as well as a non-immunologic mediated intolerance to food additives in intrinsic asthmatics, the incidence of vegetable allergies in pollen-sensitive patients, the procedures and meaning of inhalation and oral challenges, the relative importance of trophallergens. Food allergy appears to be a variable co-factor in bronchial hyperreactivity.

Asthma↗

PubMed1983

[Significance of inhaled environmental allergens].

Whereas the importance of pollen as inhalative allergens has been largely investigated and is generally known, the experience in the frequency and the role of the sensibilization with air-borne fungi is relatively limited. In 720 patients with Asthma bronchiale the degree of sensitization has been tested with various extracts of air-borne fungi of SSW Dresden (mould mixture, aspergillin, mucor, cladosporium and penicillium and alternaria). The most frequent and also the strongest reactions were found with alternaria and the smallest part of positive skin reactions with penicillium. An isolated sensitization with mould has been demonstrated in 20 per cent of the cases. In 60 per cent of the tested patients a manifest mould allergy was shown by means of the Inhalative Allergen Test, the most favourable correlation between Intracutaneous Test (ICT) and Inhalative Test (IAT) was found with alternaria (76%). A conformance between ICT and basophils degranulation test (BDT) was stated in 69% of the cases. The aim should be comparable tests with allergen extracts without irritative effects and qualitative measurements of air-borne fungi.

Air Pollution↗

PubMedDate not supplied

Penicillin allergy: in vivo and in vitro diagnostic methods.

Hypersensitivity to beta lactams, especially that mediated by IgE or IgG4, is the most frequent complaint of adverse drug reactions. There are multiple studies dedicated at establishing a method that can permit us to arrive at an accurate diagnosis, but from these studies one deduces that the difficulty lies in the lack of a test with a 100% reliability. In the present study we evaluate the correlation between the different methods. For this we selected 16 children, composed of 8 males and 8 females, whose ages ranged between 2 years 7 months and 9 years 8 months, and who had a reaction to penicillin G via parenteral. The following tests were done on them: Skin tests with a major determinant antigen (constant composition and molecular weight), with minor determinants. Human basophil degranulation test (modified Leynadier technique). Mathov's progressive controlled provocation test, which we considered the most reliable. Only in eight cases a diagnosis of penicillin allergy was arrived at. The correlation between the different tests were: Mathov - HBDT 93.75%; Mathov - PPL 93.75% and PPL - HBDT 87.5%.

Angioedema↗

PubMed1986

The migration inhibition factor test for identification of hypersensitivity reactions to drugs.

The migration inhibition factor (MIF) test detects the in vitro release of lymphokine from lymphocytes in in vitro contact with a drug that had sensitized them in vivo. The specificity and sensitivity of the MIF test in identifying a drug inducing an allergic reaction is presented. The MIF test detected the drugs responsible for 20 out of 21 allergic episodes (95.2%) while the basophil degranulation test detected only eight of them (P less than .001). The sensitivity of a positive MIF test was 95.2% and its specificity was 76.9%. The specificity of a negative MIF test was 94.7%. The positive MIF test assisted the physician in indicating the drugs responsible for an allergic reaction in half of the patients. The drugs for which the MIF test was negative could be considered innocent in 95% of the cases. It is concluded that although the results of the present studies are encouraging, the clinical utility of the MIF test is still limited and improvement of the test specificity is required.

Anti-Bacterial Agents↗