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PubMed1994

A novel method of counting eosinophils in nasal secretion of allergic rhinitis by hemocytometric method.

The test for eosinophilia in nasal secretion is a useful tool for the diagnosis of allergic rhinitis. However, the nasal smear test which is commonly used is a subjective and nonquantitative evaluation. In this paper we describe a novel simple, objective and quantitative method in which mucin cluster in collected nasal secretion or lavage is solubilized with dithioerythritol, following which the number of eosinophils per unit volume of nasal secretion or the ratio of eosinophil to total leukocyte can be successfully counted in a blood cell counting chamber by using a hemocytometric method.

Adult↗
PubMed1993

Improved Randolph stain for direct leukocyte differentiation and determination of total eosinophil count in a hemocytometer.

Identification and quantification of eosinophilic granulocytes are commonly performed indirectly by total leukocyte count and white cell differentiation in smears or cytocentrifuge preparations. Using a combination of four dyes, phloxine, Biebrich scarlet, methylene blue, and crystal violet, at 50-800 micrograms/ml, we have substantially improved an earlier method for differentiating leukocytes in a hemocytometer. This direct method allowed a rapid and reliable enumeration of eosinophils and their differentiation from neutrophils, lymphocytes and monocytes in peripheral blood and leukocyte fractions. The results obtained using this stain correlated with the leukocyte counts calculated from May-Grünwald-Giemsa stained smears in 100 patients with eosinophilia of various etiologies (r = 0.95; p < 0.01). This simple method is a useful improvement for eosinophil enumeration in field studies and biological experiments where the purity of cell suspensions must be evaluated without delay. The method cannot be substituted for the commonly used indirect technique which also allows the identification of other leukocyte lineages and their precursors.

Blood Cell Count↗