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[Sequence analysis of the fusion (F) protein genes of four Newcastle disease virus strains causing clinical disease in geese].

Four strains of Newcastle disease virus(NDV), which have proven to be responsible for the disease outbreaks in south and east China regions called "geese paramyxovirus infection" or "avian paramyxovirus infection of geese", were examined for the features of their fusion(F) protein genes. A 1700 nucleotides(nt) fragment of the F gene (1705 nt) of each of the 4 strains were sequenced, the results revealed that the NDV strains of goose origin share common transcriptional sequence and translational start position with the hitherto reported NDV, the F gene homologies among the 4 strains were 97.8%-98.9%, while the corresponding homologies between these strains and the standard challenge strain F48E8 were only 86.0-86.8%. The deduced amino acid sequences near cleavage site of F0 proteins showed a 112RRQKR/F117 motif, comforming well with that of the virulent strains. The cleavage sites distribution of 3 restriction enzymes(Hinf I, Basto I, Rsa I) in 75% region of the F gene(between 334-1682 nt), which is used to group NDV strains into different genotypes, were also analysed with the aid of computer program MegAlign, it is very interesting to find that the 4 strains showed a distinct distribution pattern from all the genotypes so far reported(genotype I-VIII), characterized by the simultaneously presence of a Bsto I site at 752 nt and two Rsa I sites at 872 nt and 973 nt. Additionally, 3 out of the 4 strains, despite their different geographical locations, showed a novel Rsa I site at position 1249 which is absent in all of the other genotypes. The 4 NDV strains of goose origin were essentially virulent ones, and probably belong to a new genotype.

Amino Acid Sequence↗

An outbreak of West Nile virus-associated disease in domestic geese (Anser anser domesticus) upon initial introduction to a geographic region, with evidence of bird to bird transmission.

West Nile virus activity in Manitoba was documented for the first time by the collection of an infected crow found on July 8, 2002, in Winnipeg. West Nile virus was identified as the cause of death for a large number of domestic geese at a single farm in southern Manitoba in August. Of the 5 differently aged cohorts on the affected farm, which included 2 breeding flocks and 3 growing flocks, the 6-week-old cohort was most severely affected with 692 of 2731 goslings dying within a 10-day period. Seroprevalence of West Nile virus in 2 clinically affected and recovered juvenile cohorts was 98% and 100%. In breeding geese without clinical disease, seroprevalence was 90% for 15-month-old birds and 10% for 5-year-old birds. Seroreaction in 3 of 4 cohorts tested exceeded what would be expected by mosquito transmission alone.

Animals↗

Age-related variations in some blood parameters of geese.

Blood samples from a total of 44 geese of the Hungarian breed, representing three age groups (3 months old, 6 months old, and adult) and originating from the Babat Goose Farm, were examined for packed cell volume (PCV), differential count, haemoglobin content, and serum levels of glucose, total protein, total lipid, calcium and phosphorus. The results revealed significant age-related variations in PCV, proportions of lymphocytes and eosinophils, total protein, total lipid, calcium and phosphorus. The changes were comparable to those obtained for 3 months old and adult Landes geese.

Aging↗

Effects of dietary protein deficiency on hepatic carbohydrate metabolism in geese.

This paper was aimed at following up the course of glucose in the liver of protein underfed geese. Compared to normally fed geese, liver carbohydrate metabolism in protein underfed animals can be characterised by lowering of free glucose content of in vitro glucose uptake and glycogen synthesis and reduction of carbohydrates in total oxidation. Glycogen content of the liver and rate of glucose oxidation were also lowered, but these differences were not statistically significant. Summing up our results and taking into consideration some literature data, an overall reduction of hepatic carbohydrate metabolism in protein underfed birds may be assumed.

Animals↗

Determination of a sedative dose and influence of midazolam on cardiopulmonary function in Canada geese.

Midazolam HCl (1.0 or 2.0 mg/kg of body weight) was administered IM to 6 Canada geese to determine a sedative dose that would allow positioning for radiologic examination. The effects of both test doses on cardiopulmonary function were evaluated at 5, 10, 15, 20, 30, and 40 minutes after drug administration and were compared with 2 end-tidal isoflurane concentrations (1.5 and 2.5%). The 2.0 mg/kg dosage induced moderate sedation at 15 and 20 minutes; sedation was adequate for positioning the geese. Sedation induced by the 1.0 mg/kg dosage was inadequate. The effects of both test doses on blood pressure, heart rate, and temperature were not significantly different from each other and from baseline data. Respiratory rate increased significantly (P less than 0.05) at 10, 15, 20, and 30 minutes with the 2.0 mg/kg dosage, and at 15 and 20 minutes with the 1.0 mg/kg dosage. Blood pressure and respiratory rate were significantly (P less than 0.05) decreased with isoflurane when compared with baseline data and the midazolam test doses. The results of this study indicate that midazolam at a dosage of 2.0 mg/kg induces adequate sedation with minimal cardiopulmonary changes, and, as an alternative to general anesthesia with isoflurane, provides a satisfactory level of restraint for radiography.

Animals↗

[Quality and nutritive value of the liver from fattened local geese].

A research-and-economic experiment was carried out with a total of 8,621 geese of local breeds of 3.964 kg average body mass, fattened forcedly with steamed grain maize in the course of 20-30 days. The dynamic of a number of qualities was followed up with special emphasis on the live weight from the beginning to the end of the fattening period, the weight gain, the mass and quality of liver, and the intake of feed per kg of gain and per kg of liver mass. Determined was also the nutritive value of proteins contained in the liver as judged by the index of the amino acid ratio (tryptophan to hydroxyproline). It was found that local geese could be characterized by good fattening capacity, yielding high-quality liver of excellent organoleptic indices and high nutritive value at a comparatively low intake of feed.

Animals↗

Influence of breeding technology of helminth fauna of geese (Anser anser f. domestica).

Two hundred goose broilers originating from farms with different housing facilities were examined for helminth parasites. The farm of the first technological type consisted of halls with deep liter without a run and that of the second type had grass runs neighbouring with a sheet of water. The geese from the first farm harboured A. galli, H. gallinarum and C. obsignata; those from the second farm, D. lanceolata, H. dispar, A. anseris and C. obsignata. In the first case, the geese were infected by helminths from chickens. The incidence and intensity of infection, frequency of single and mixed infections and relation between the incidence and intensity of infection and the age of broilers were evaluated. The influence of breeding technology on the formation of helminth fauna and the significance of individual helminth species in goose breeding are discussed.

Animals↗

Prevalence of Salmonella contamination in market-ready geese in Manitoba.

Salmonella contamination is a major problem in the poultry industry, but no data are available on the prevalence in geese. From two slaughter facilities in Manitoba, 130 carcass rinsings and 315 cloacal swabs from a total of 17 flocks of domestic geese were examined for salmonellae. Sixty percent of the carcass rinsings and 18.4% of the cloacal swabs were positive. Most of the isolates were serotyped, and some analysis of the results is presented. Three culture media were compared, and a modified dulcitol brilliant-green novobiocin medium was found to be more selective than brilliant-green and brilliant-green sulfa agars.

Animals↗

An outbreak of duck virus enteritis among ducks and geese in Denmark.

Duck virus enteritis occurred in the spring of 1982 among domesticated mallards, Pekin ducks and geese producing eggs for the same hatchery. Wild mallards may have introduced the infection to the domestic birds. High mortality occurred in one flock of Pekin ducks and in young geese. Mallards were also affected, but less severely. Gross and microscopic lesions were in general typical for DVE. Virus was demonstrated by electron microscopy of Bursa fabricii from experimentally infected ducklings. Neutralizing antibodies were found in serum from ducks, surviving an acute outbreak in the flock. Vaccination was performed and hygienic precautions taken, and transmission from infected flocks to progeny was negligible.

Animals↗

Anthelmintic activity of flubendazole in naturally infected geese and the economic importance of deworming.

The efficacy of flubendazole food medication against roundworms in geese was determined under controlled field conditions. The geese were infected with Amidostomum anseris, Capillaria anseris, Trichostrongylus tennis, and Syngamus trachea. Medicated food was given for 7 consecutive days at a level of 30 ppm. Anthelmintic activity, egg production, and hatchability were recorded. Flubendazole medication resulted in a 100% worm elimination, no consistent change in egg-lay performance, and a highly significant increase in hatchability.

Animals↗

[Helminths in broiler geese fattened in runs].

The occurrence of helminths in broiler geese was studied in the rapid-fattening technological system in 1974-1978. The birds were kept on grassy run adjacent to water. Helminthological examination was performed in 1065 broilers and the following 16 helminth species were revealed: Notocotylus attenuatus (0.3%), Apatemon gracilis (1.5%), Cotyrulus cornutus (0.1%), Hypoderaeum conoideum (0.1%), Retinometra longicirrosa (3.2%), Drepanidotaenia lanceolata (3.1%), Sobolevicanthus fragilis (0.7%), Diorchis stefanskii (0.8%), Microsomacanthus microsoma (0.9%), Dilepis undula (0.1%), Capillaria caudinflata (0.2%), Capillaria obsignata (7.7%), Amidostomum anseris (3.7%), Trichostrongylus tenuis (0.2%), Ganguleterakis dispar (16.8%), and Heterakis gallinarum (0.4%). Seven of these species were found for the first time in geese in Czechoslovakia (A. gracilis, C. cornutus, H. conoideum, M. microsoma, D. undula, C. caudinflata, and H. gallinarum). The species Dilepis undula has not been recorded in this host species ever before. The extensity and intensity of the invasions, the frequency of monoinvasions and mixed invasions, and the relation of invasion extensity and intensity to the age of the broilers examined were evaluated during the study.

Animal Husbandry↗

[Development of morphological changes after experimental Mycobacterium avium infection in ducks and geese].

Ducks and geese were experimentally infected with the Mycobacterium avium. It was demonstrated that intramuscular and peroral infection causes morphological tuberculous changes. The first microscopically detectable changes induced by intramuscular infection occurred within 12 days. After 70 days from infection the affected organs were densely covered with tuberculous lesions. The development of microscopic changes is continuous and the development of affections is accompanied by an increase in the number of mycobacteria detectable in the sections. In ducks this trend is more pronounced than in geese. In both methods of infection liver and spleen are the first and the most frequent organs to be affected.

Animals↗

[The aerobic bacterial intestinal flora of various wintering geese species].

The aerobic fecal flora of wintering Brent Goos (Branta bernicla), Barnacle Goose (Branta leucopsis), Greylag Goose (Anser anser), White-fronted Goose (Anser albifrons), Pink-footed Goose (Anser brachyrhynchus), and Bean Goose (Anser fabalis) was studied. There were no specific differences between the various geese. Bacterial counts were in the range of 10(5)-10(7) CPU per gram of feces. Neither pathogenic bacteria nor rotavirus could be detected in the fecal samples of the wintering geese, so that a contamination of the environment with those pathogenic organisms could be excluded. The majority of the isolated bacteria belonged to the genera Bacillus and Pseudomonas; enterobacteria and streptococci were less common. The observations are discussed regarding their epidemiological and ecological significance.

Aerobiosis↗

Light and electron microscopic investigations on stellate cells in pars distalis of the adenohypophysis of ducks and geese.

The pars distalis of geese and ducks was investigated by light and electron microscopical methods to identify certain peculiarities of the stellate cells. Stellate cells were found in both cephalic and caudal lobes of the pars distalis in both sexes of geese and ducks. These cells were small in size and stained blue to violet by Performic-Acid, Alcian- blue (pH 0.2), PAS, Orange G stain. The stellate cells have numerous fine and long cytoplasmic processes which are linked together and with the pars distalis cells by cell junctions. Some of the stellate cells were arranged into folliclelike structure entirely surrounding an extra-cellular heterogenous substance. Particular emphasis was given to the ultrastructure peculiarities of the stellate cells which may be considered to exhibit secretory as well as phagocytic activities.

Animals↗

Pathomorphological changes caused by T-2 trichothecene fusariotoxin in geese.

T-2 trichothecene fusariotoxin was administered to 110 laying geese and 33 ganders in the active egg production period through a tube at 2-day intervals on a total of 10 occasions. After the treatment, the geese were subjected to detailed pathomorphological examination. In the ovaries of layers, a cessation of follicle maturation and follicle degeneration dependent on the toxin dose were observed, accompanied by ovulation and consequent peritonitis in the birds that died and in some of those killed by bleeding. Additional findings included involution of the oviduct, lymphocyte depletion, necrosis and amyloidosis in the spleen, catarrhal enteritis, signs of colloid stasis in the thyroid and large numbers of secretory granules in the cytoplasm of the adrenaline-producing cells of the adrenal gland. In the ganders, toxin administration did not reduce the intensity of spermatogenesis but in the spleen, intestine and endocrine glands it caused changes similar to those seen in the layers.

Animals↗

The relationship between social stress and dominance is seasonal in greylag geese

Studies of captive animals have suggested that social stress affects subdominants, whereas recent data from the wild have revealed that stress mainly affects dominants. We used a non-invasive approach to investigate, for the first time in a social bird, the circannual stress-dominance relationships between low-ranking single males, intermediately positioned paired ganders without offspring and high-ranking paired males with offspring from a flock of semi-tame, free-ranging greylag geese, Anser anser. We collected 933 faecal samples from 43 individuals, 12 singletons, 18 paired males without offspring and 13 paired males with offspring over an entire year and analysed them for corticosterone metabolites by enzyme immunoassay. During the mating season (February-April), singletons had marginally higher corticosterone than paired males (P<0.1), whereas during the parental season (May-January), the paired males with offspring had significantly higher corticosterone than both paired males without offspring and singletons. All three male categories had significantly higher corticosterone during the mating season than during the rest of the year. These results suggest that social stress in ganders is caused mainly by competition between males and by constrained access to females during the mating season, but by parental commitment during the rest of the year. We suggest that dominance per se may not be a direct cause of stress. Rather, the amount of social stress may co-vary with the behavioural investment individuals need to make to optimize their fitness and with the relationship between such demands and the individuals' rank positions. This relationship seems to be seasonal in geese. Copyright 1998 The Association for the Study of Animal Behaviour.

Journal Article↗

Microbial impact of Canada geese (Branta canadensis) and whistling swans (Cygnus columbianus columbianus) on aquatic ecosystems.

Quantitative and qualitative analyses of the intestinal bacterial flora of Canada geese and whistling swans were carried out with the finding that wild birds harbor significantly more fecal coliforms than fecal streptococci. The reverse was typical of captive and fasting birds. Neither Salmonella spp. nor Shigella spp. were isolated from 44 migratory waterfowl that were wintering in the Chesapeake Bay region. Enteropathogenic Escherichia coli were detected in seven birds. Geese eliminated 10(7) and swans 10(9) fecal coliforms per day. Results of in situ studies showed that large flocks of waterfowl can cause elevated fecal coliform densities in the water column. From the data obtained in this study, it is possible to predict the microbial impact of migratory waterfowl upon aquatic roosting sites.

Animals↗

Estimation of genetic parameters for growth, carcass and overfeeding traits in a white geese strain.

In an experimental strain of white plumage geese created in 1989, two experiments were carried out from 1993 to 1995 in order to estimate genetic parameters for growth, and carcass composition traits in non-overfed animals, and genetic parameters for growth and fatty liver formation in overfed animals. Four hundred and thirty-one non-overfed animals were bred and slaughtered at 11 weeks of age; they were measured for forearm length, keel bone length, chest circumference and breast depth before and after slaughtering. The carcasses were partly dissected in order weigh breast, breast muscle and skin + fat, and abdominal fat. Four hundred and seventy-seven overfed animals were slaughtered at 20 weeks of age; they were measured for "paletot" (breast meat, bone and meat from wings, bone and meat from thigh and legs) weight and liver weight. In these two experiments, the weights had moderate to high heritability values. Breast depth measured on live animals showed a low heritability value. In overfed animals, liver weight showed a high heritability value. Liver weight could be increased by selection without a great effect on "paletot" weight. Thus, obtaining a white plumage geese strain for fatty liver production by selection would be difficult because only 20% of overfed animals had fatty liver. The results did not allow to conclude on the influence of selection on liver weight on carcass traits such as muscle or fatty tissue weight.

Journal Article↗