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[Investigation of initial stage of fibroblast attachment by means of interference reflection microscopy].

The initial cell--substrate contach, arising before the beginning of fibroblast spreading is formed from the multitude of discrete microcontacts (MC). The number of these MC increases monotonously and at room temperature 20 degrees C reaches the constant maximum value three hours after the cell settling from the suspension. An increase of MC number is accompanied by two phenomena: 1) expansion of the MC zone from the cell pole to periphery; 2) the following formation of the central continuous contact as a consequence of the fusion of the adjacent discrete MC. The zone of continuous contact expands also from the pole to periphery. The quantitative characteristics of this process are presented and the model of initial attachment as the successive attachments of the cell surface projections are discussed.

Animals↗
PubMed1973

Differential interference contrast microscopy of human atherosclerotic lesions.

Differential interference contrast microscopy was employed to study sections of human cerebral arteries and aorta. When this procedure was used to observe 0.5-mu-thick sections of plastic-embedded arteries, images were obtained with greater definition of detail than corresponding micrographs of the area using conventional bright-field microscopy. Since structures with different refractive indices are shown in geometric relief from one another, an apparent three-dimensional image is seen, which, together with the theoretically higher resolution and contrast achieved by this technic, gives an image with definition between conventional light and low magnification electron microscopy. The morphology of fatty streak lesions with aggregates of round and elongated lipid-filled cells was demonstrated, and clear images of different forms and sizes of intracellular lipid droplets were illustrated at magnifications around 1000. In atheromatous plaques, intra- and extracellular lipid droplets were observed, some with partially extracted cores or surfaces, as were amorphous lipid droplets coalescing with crystals whose centers had been extracted. We conclude that this optical accessory to a research photomicroscope is a valuable supplemental tool in studies on the morphology of atherosclerotic lesions.

Aorta↗
PubMed1988

The Bacteroides glycocalyx as visualized by differential interference contrast microscopy.

The glycocalyx of eight strains representing six species of Bacteroides was examined by differential interference contrast microscopy. Wet mounts in India ink were prepared from bacteria cultured in broth and on an agar medium; the wet mounts were observed by phase-contrast microscopy and differential interference contrast microscopy. With differential interference contrast microscopy, all bacteria demonstrated a glycocalyx, which included capsules surrounding single cells and microcolonies, strands of glycocalyx connecting cells and microcolonies, detached slime, and solid masses of glycocalyx in which innumerable bacteria were enmeshed. Bacteria showed comparable amounts of glycocalyx by visual observation with differential interference contrast microscopy whether grown on plates or in broth. Serial transfers of cultures did not diminish the amount of glycocalyx. Differential interference contrast microscopy proved to be a superior method to phase contrast for examining wet preparations of Bacteroides.

Bacteroides↗
PubMed1999

Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy.

Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and shear. Results show quantitative and qualitative agreement between model and data for several imaging conditions.

Evaluation Studies as Topic↗