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At least 163 records · Page 9Linked to original sources
PubMed1995

A method of short-term cryostorage and selection of viable sperm for use in the various assisted reproductive techniques.

The objective of this study was to determine if spermatozoa, following short-term cryostorage at 5 degrees C in Test-Yolk buffer (TYB; ZBL, Inc., Lexington, KY, USA), could be recovered and improved via the SpermPrep filtration method and to assess the possibly enhanced fertilizing capacity of the selected spermatozoa. Semen specimens from 20 men were collected, evaluated, diluted 1:1 (v/v) with TYB, divided into aliquots and cooled to 5 degrees C for 24 and 48 hr. Semen samples were assessed for volume, sperm count, percentage and grade of motility, percentage of morphologically normal spermatozoa and outcome of the sperm penetration assay (SPA). After storage, aliquots were rewarmed at 37 degrees C, centrifuged, and the pellet was resuspended in 1.0 ml of SpermPrep media (ZBL, Inc.). Following 15 min of incubation, the rewarmed spermatozoa were filtered via the SpermPrep I filtration column (ZBL, Inc.) and assessed accordingly. The results obtained in this study indicate that the short-term cryostorage procedure yielded spermatozoa of adequate qualitative characteristics when compared to the fresh spermatozoa. Furthermore, filtration of rewarmed specimens yielded spermatozoa of significantly higher qualitative characteristics and superior fertilizing capacity following a short-term cryostorage period in TYB when compared to fresh and rewarmed spermatozoa (p < 0.05). This method of short-term cryostorage in TYB and selection of superior spermatozoa via the SpermPrep filtration method could further enhance the fertilizing ability of patients who produce spermatozoa characterized by deficient capacitation, acrosome reaction and subsequent fertilization.

Cryopreservation↗
PubMed2004

Efficacy of recombinant human follicle-stimulating hormone in improving oocyte quality in assisted reproductive techniques.

OBJECTIVE: To estimate the efficacy of recombinant human follicle-stimulating hormone (rFSH) versus highly purified urinary human FSH (uFSH) in women undergoing controlled ovarian hyperstimulation (COH) for in vitro fertilization and embryo transfer (IVF-ET). STUDY DESIGN: This prospective, randomized, quality of retrieved double-blind study compared uFSH with rFSH in IVF-ET rFSh cycles. A total of 254 cycles from 241 patients who attended the infertility clinic at Samsung Cheil Hospital from January to August 2001 were included in the study. With pituitary desensitization using gonadotropin-releasing hormone agonist and a short protocol, rFSH was administered in 131 cycles; uFSH was administered in 123 cycles. We analyzed ovarian response, oocyte quality, fertilization rate, embryo quality, pregnancy rate and live birth rate in the uFSH and rFSH groups. RESULTS: Total FSH dosage (1322.3 +/- 526.2 vs. 2124.4 +/- 881.9 i.u.) and dosage per retrieved oocyte (90.6 +/- 36.0 vs. 138.0 +/- 57.2 i.u.) were significantly lower in the rFSH group than those in the uFSH group (P < .001). The proportion of good-quality oocytes (grade 1 and 2) was higher in the rFSH group (68.2% vs. 64.8%, P = .024). Moreover, the proportion of atretic oocytes (grade 5) was lower in the rFSH group (7.5% vs. 10.3%, P = .002). The fertilization rate, quality of transferred embryos, clinical pregnancy rate and live birth rate were not significantly differ between the 2 groups. CONCLUSION: In women undergoing COH, rFSH revealed more efficient ovarian response and better quality of oocytes than did uFSH.

Adult↗
PubMed2004

[In vitro fertilization laboratory. Clinical features of assisted reproduction techniques. Male infertility treatment in a reproduction medicine centre].

The development of fertilization techniques such as ICSI, complementary to conventional in vitro fertilization, have been a great advance in the treatment of the male factor. ICSI with spermatozoa from ejaculate allows successful treatment of severe male factor, patients with previous failures of fertilization, and also cases without apparent cause. ICSI with spermatozoa obtained directly from the testicle allows couples in which the male suffers azoospermia, either obstructive or secretory, achieving pregnancy. After ICSI, pregnancy rates are similar to the ones after conventional in vitro fertilization. It is necessary to evaluate the genetic risk for children born after ICSI. On the one hand there are anomalies bound to subfertile population they come from, on the other chromosome anomalies generated de novo. ICSI does not increase the incidence of major malformations. It is recommendable to perform prenatal diagnosis in pregnancies obtained by ICSI.

Chromosome Aberrations↗
PubMed1994

[Echography in assisted reproduction techniques].

The Authors describe the A.R.T. echoguided techniques, that can be routinely used in IVF/ET programs. All these techniques are simple and have replaced the previous ones. The echoguided oocyte recovery is now widely accepted instead of the laparoscopic way: the gamete intrafalloppian transfer can be easily performed under local anesthesia; the selective fetal reduction in cases of multiple pregnancies can be easily carried out on an outpatient basis.

Female↗
PubMed1994

Assisted reproductive techniques and male infertility.

For couples in whom improvement in spermatogenesis is not possible or is unsuccessful in producing conception, ARTs offer much promise. For mild to moderate male factor couples, IUI combined with superovulation is often the initial procedure employed. If unsuccessful, IVF or one of its variations should be considered. With severe male factor cases, couples should proceed directly to IVF or one of its variations in which specific techniques may be used to improve the chances of fertilization. Currently both specialized semen-processing techniques and micromanipulation are used either singly or in combination. More experience is needed to determine which couples will benefit from which techniques. Although the urologist may not actively participate in all the actual ART procedures, it is important that the urologist have a thorough understanding of the procedures and their indications. This allows for accurate counseling of the male factor couple.

Humans↗
PubMed1996

[Cryopreservation of embryos--used for treatment of infertility by means of assisted reproductive techniques (ART)].

Freezing of the human embryos has been routinely used in the world for over 10 years in the treatment of infertility by the ART. Excessive fertilized oocytes and embryos can be frozen and preserved in the temperature of liquid nitrogen. The risk of multiple pregnancies is reduced and enhancement of pregnancy rates can be obtained owing to cryopreservation. The author presents theoretical principles of embryo freezing by slow cooling with 1, 2 propanediol and saccharose as a cryoprotectant.

Cryopreservation↗
PubMed1996

[1995 evaluation of the CECOS Federation. Use of different methods of assisted reproductive techniques].

Intracervical insemination IC is still the most common method of ART with frozen donor semen. During the year 1995 in France IC was used in 63% of all cycles, with a pregnancy rate per cycle (PR) of 9.3%. Intrauterine insemination IU was used in 24% of all cycles with a PR of 13% (p < 0.01) and in vitro fertilization IVF in 12% of all cycles with a PR of 22% (p < 0.01). The benefit of the different methods, in relation with the number of unsuccessful cycles, and in relation with the quality of frozen semen is analysed.

Female↗
PubMed1996

Use of assisted reproductive techniques for treatment of ejaculatory disorders.

Ejaculatory disorders can interfere with the fertility of young adults who suffer from spinal cord injury, have type I diabetes mellitus or have undergone retroperitoneal or intrapelvic operations. Following an overview of causes and treatment of ejaculatory disorders, the data of our centre are presented. From a group of 37 patients with genuine loss of seminal emission, 15 men and their wives were offered a combined treatment of rectal probe electro-ejaculation and artificial reproductive techniques. No serious complications occurred. During 40 cycles with intracorporeal insemination and 11 cycles with extracorporeal fertilization techniques, seven pregnancies were achieved, representing a pregnancy rate of 46% per couple and 14% per cycle for all cycles. Five healthy children were born, all following extracorporeal insemination. The 'take-home baby rate' for this population and for this technique is 45%. In vitro fertilization (IVF) led to one birth, intracytoplasmic sperm injection (ICSI) achieved four live births out of three pregnancies, one being a twin gestation. Since our successes are due to the use of extracorporeal insemination techniques, these are now incorporated in a new, more rational treatment protocol.

Ejaculation↗