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PubMedDate not supplied

[The virulence of Yersinia pestis strains in serial-passage in guinea pig peritoneal macrophages].

The cultivation of Y.pestis virulent strains in macrophages of guinea pigs has been found to enhance their virulence, decreased during storage, and to increase their antiphagocytic and cytopathic properties. This effect makes it expedient to recommend the passage of Y.pestis through peritoneal macrophages of guinea pigs with the aim of stabilizing their virulence. The proposed method reduces the duration of investigation 6 times, decreases the number of experimental animals used in the investigation and permits the simultaneous passage of a large number of subcultures.

Animals↗
PubMedDate not supplied

[The ultrastructural changes in guinea pig organs during the serial passage of the Ebola virus].

Morphological study of internal organs of guinea pigs inoculated with Ebola virus at 2-8 passages was carried out. In the course of these passages the number of infected cells and virus particles in the organs was shown to increase, and the destructive changes in organs became more pronounced. In the 1st-3rd passages Ebola virus replication was observed in macrophagal cells only but beginning from the 4th passage of virus reproduction was found also in hepatocytes, spongiocytes, and fibroblasts.

Animals↗
PubMed1989

[The effect of serial passage of Coxiella burnetii in chick embryos on pathogen phase variability].

Coxiella burnetii has been investigated in different egg passages (EP-17, 163). Coxiella in EP 163 was completely eliminated from the suspension during the purification but not that in EP 17. It means that the coxiella of the two EP with a seroreaction of phase II are different, and phase II positivity of EP 17 can't be the result of presence of two populations in the suspension or of one mutation during the multiplication in yolk sacs but of a change on the surface of C. burnetii.

Animals↗
PubMed1993

An alternative protocol for the serial passage and maintenance of a homogonic strain of Strongyloides ratti in female Sprague-Dawley rats.

A method which does not involve the tedious use of watch glass coprocultures for obtaining filariform infective (L3) larvae of Strongyloides ratti from faecal pellets of infected Sprague-Dawley rats is described. The alternative method utilises Baermannization (18 h) of faecal pellets to yield rhabditiform (L1) larvae of S. ratti and their subsequent culture for 72 h at 19 degrees C in tissue-culture-flasks containing only dechlorinated tap water to yield infective filariform (L3) larvae. The yields and infectivity of the L3 larvae obtained from the two methods were essentially similar.

Animals↗
PubMed1996

Serial passage of human immunodeficiency virus type 1 generates misalignment deletions in non-essential accessory genes.

Human immunodeficiency virus type 1 (HIV-1) derived from an infectious molecular clone pNL432 was extensively passaged in tissue culture by repeated rounds of acute infection. We previously showed the natural occurrence of a nonsense mutation in the vpr gene during continued passage of this virus. In this report, we show that two forms of large deletions (561 and 518 base pairs containing short direct repeats at the deletion junctions) occur after passage 50 in the region that spans the vif and vpr open reading frames. One model to explain the occurrence of these deletion regions is that such mutations result from misalignment of the growing point at a limited number of nucleotide positions. Infection of CD4+ T-cells with a recombinant HIV-1 construct containing the same vif to vpr deletion showed virtually no cytopathogenic phenotype. Thus, misalignment deletions at non-essential accessory genes of HIV-1 might be induced during replication, which result in the generation of virus with a low cytopathogenic potential.

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