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PubMed2000

Noninvasive determination of hemoglobin and hematocrit using a temperature-controlled localized reflectance tissue photometer.

We performed visible/near-infrared optical measurements on the forearm of human subjects. We conducted four studies: one study using a commercial diffuse reflectance spectrometer, and three studies using a breadboard temperature-controlled localized reflectance tissue photometer. Calibration relationships were established between skin reflectance signal and either the reference blood hemoglobin (Hb) concentration or the hematocrit values (Hct). Prediction results were expressed as the prediction correlation coefficient (r(p)) and the standard error for cross-validation prediction (CV-SEP). Using diffuse reflectance measurement, r(p) = 0. 8, CV-SEP = 0.9 g/dL for Hb and r(p) = 0.7, CV-SEP = 3.3% for Hct (n = 40). In a localized reflectance study involving calculating the absorption and scattering coefficients and including effect of change of skin temperature in the calibration model, the best prediction results were r(p) = 0.9, CV-SEP = 0.8 g/dL for Hb and r(p) = 0.8, CV-SEP = 3% for Hct (n = 26). In a second localized reflectance study on a population having diverse skin colors at 34 degrees C, the optimal model led to r(p) = 0.8, CV-SEP = 0.9 g/dL for Hb and r(p) = 0.9, CV-SEP = 2.1% for Hct (n = 28) using the localized reflectance values without deducing the absorption and scattering coefficients. Improvement in the correlation was more noticeable for Hct than for the case of Hb. The photometer was used to screen prospective blood donors with low Hb concentration. It was possible to predict anemic subjects in the limited prospective blood donor population.

Calibration↗

PubMed1995

Reflection-polarization patterns at flat water surfaces and their relevance for insect polarization vision

It has recently been shown that horizontally polarized ultraviolet light reflected from the surface of water is the main optical cue for habitat finding by insects living in, on, or near water. What are the polarization properties that make the skylight reflected by water attractive to flying water insects in nature? In this paper, as an approach to this problem, the patterns of the degree and direction of polarization of skylight visible over a flat water surface are computed for unpolarized light from an overcast sky and for partially polarized skylight as a function of the zenith distance of the sun. These patterns are compared with the corresponding celestial polarization patterns. The effect of depolarizing clouds on these reflection-polarization patterns is demonstrated. Reflectivity patterns of a flat water surface are also calculated for clear and overcast skies. The polarization of the blue sky is described by the semi-empirical Rayleigh model. It is assumed that the reflection polarization of skylight at the water surface is governed by the Fresnel formulae. The effect of some modifying factors on the reflection-polarization field is briefly discussed. The adaptations of the visual system of insects living in, on, or near water to reflection-polarization patterns at water surfaces are briefly reviewed and discussed by means of three representative species: the waterstrider (Gerris lacustris), the backswimmer (Notonecta glauca), and the dragonfly (Hemicordulia tau). Copyright 1995 Academic Press Limited

Journal Article↗

PubMed1995

Reflective organelles in the anterior pigment epithelium of the iris of the European starling Sturnus vulgaris.

The ultrastructure and chemical composition of reflective organelles in the anterior pigment epithelium of the iris of the European starling Sturnus vulgaris were examined. The reflective organelles produced a diffuse white reflectance at the iris mid-section which was visible only when the stroma was removed. The pigment granules were clear, angular, and birefringent under the light microscope. In electron micrographs the granules were irregular in shape and density, sometimes crystalline in appearance, but more often they were lost during sectioning or staining. Guanine was abundant in the modified pigment epithelium of the starling, but not in the pigment epithelia of other birds that lacked birefringent granules. Pteridines, such as xanthopterin and leucopterin, were present in small amounts. Pteridines were also present in the iris stroma which had no reflective organelles. The reflective organelles in the starling pigment epithelium resemble both the reflecting platelets of lower vertebrate chromatophores and the reflective granules in the tapeta of various vertebrates. Possible derivation of the organelles from these sources is discussed.

Animals↗

PubMed1999

Sex and age-specific differences in ultraviolet reflectance of scent marks of bank voles (Clethrionomys glareolus).

Scent markings of voles are visible via their ultraviolet reflection. Kestrels, and possibly other diurnal raptors, may use this property when hunting. We performed a laboratory study on bank voles to determine whether UV-reflectance of scent marks differs in relation to sex, age and social status. When reflectance spectra of scent marks were measured with a spectro-radiometer, we found UV reflectance to be strongest in mature males. There were no differences between mature females and immature juveniles, nor between sexes in juveniles or mature and immature individuals in females. Moreover, we did not find any difference in UV reflectance between dominant and subordinate mature males. The results of this study support earlier findings that UV sensitive predators may use UV reflectance of scent marks as a prey cue. Consequently, studies on differing vulnerability of voles to avian predators should take into account not only their space use and behaviour but also the UV reflectance of their scent marks.

Aging↗

PubMed1994

The Clinical Learning Spiral: a model to develop reflective practitioners.

Reflective practice in clinical nursing is an exciting concept. Much of the literature on reflection has been derived from education. Recently the Australasian Nurse Registering Authority Committee (ANRAC) endorsed reflective practice as a registering prerequisite competency for beginning nurse practitioners. This paper examines the concept and development of an action research clinical learning spiral to foster reflective practice of both undergraduate students and their clinical teacher in the practice setting. The innovation of a mutual group, that is, teacher and students interacting through reflection to create a co-operative learning environment is explored. In designing the spiral a number of models were consulted and incorporated. The action research clinical learning spiral adds structure and focus to the process of reflection-on-action and provides an avenue for students and the clinical teacher to set mutual goals of action to trial for future experiences. This process of reflection allows the clinical facilitator to be an integral component of success to the students learning in the clinical context.

Clinical Competence↗

PubMed1994

Reflective practice in nursing: issues and implications for nurse education.

Reflective practice is a frequently used but inadequately defined concept in nursing. Part of the reason for this may be the inadequate conceptualisation of the process of reflection. This paper argues that now is an appropriate time to critically examine the notion of reflective practice and maintains that there is a need for more debate and research into the nature of reflection in nursing. This paper reviews recent nursing ideologies and explores the concept of reflective practice in relation to different forms of practical knowledge. It discusses and critically analyses the attraction of reflective practice to different interest groups and concludes by examining the implication of reflective practice models of nursing for nurse educators.

Education, Nursing↗

PubMed2003

Reflection of structural waves at a solid/liquid interface.

This paper investigates the reflection characteristics of structural or guided waves in rods at a solid/liquid interface. Structural waves, whose wavelengths are much larger than the diameter of the rod, are described in a first approximation by classical one-dimensional wave theory. The reflection characteristics of such waves at a solid/liquid (melting) interface has been reported by two different ultrasonic measurement techniques: first, measuring the fast regression rate of a melting interface during the burning of metal rod samples in an oxygen-enriched environment, and second, monitoring the propagation of the solid/liquid interface during the slow melting and solidification of a rod sample in a furnace. The second work clearly shows that the major reflection occurs from the solid/liquid interface and not the liquid/gas interface as predicted by plane longitudinal wave reflectivity theory. The present work confirms this observation by reporting on the results of some specially designed experiments to identify the main interface of reflection for structural waves in rods. Hence, it helps in explaining the fundamental discrepancy between the reflection characteristics at a solid/liquid interface between low frequency structural waves and high frequency bulk waves, and confirms that the detected echo within a burning metallic rod clearly represents a reflection from the solid/liquid interface.

Aluminum↗

PubMed1998

Shape from shading: estimation of reflectance map.

The reflectance map used by the visual system for perception of shape from shading was estimated. In Experiment 1, an image of a cylinder or a sphere illuminated from the viewer's direction was presented, and subjects estimated the cross-section of perceived 3D-shape. The reflectance map was estimated from the relationship between the stimulus image intensities and the slants of the measured cross-section. The estimated reflectance maps were not the ones based on Lambertian reflectance properties. In Experiment 2, whether perceived shapes could be predicted based on the reflectance maps obtained in Experiment 1 was examined. Subjects performed the same shape estimation task with images of cylinders generated by the reflectance map obtained in Experiment 1. The perceived shapes coincided well with the shapes used for stimulus image generation. These results indicate that the visual system's estimation of shape from shading can be fully understood based on empirically obtained reflectance maps without mentioning its inaccurate nature which has been claimed by past studies.

Algorithms↗

PubMed1993

Reflection: a review of the literature.

This paper is a review of the literature on reflection. The purpose was to unravel and make sense of the complex literature, and to identify the skills required to engage in reflection. An analysis of the literature revealed that differences between authors' accounts of reflective processes are largely those of terminology, detail and the extent to which these processes are arranged in a hierarchy. Key stages of reflection are identified and represented by a model. Skills required to engage in reflection were found to be implicit in the literature and these are identified. Methodological issues related to empirical literature are discussed. It is suggested that reflection is an important learning tool in professional education and that the skills required for reflection need to be developed in professional courses.

Humans↗

PubMed1995

Hindsight bias in reflective practice: an empirical investigation.

Reflection is seen as a central component in the education and practice of nurses. It is through critical reflection on one's practice that expertise can be assured. This faith in the process of reflection has influenced much of pre and post registration nurse education. It is only in the past few years that reflection as a technique has being criticized. A small-scale study investigating the phenomenon of hindsight bias and its consequence on the reflective process is described. The effects of the hindsight bias is to influence people's recollection of events once they know the final outcome. The present study does indicate that nurses are susceptible to such a bias, which would question the validity of reflection as a way to enhance patient care. There is an urgent need to clarify the reflective process and then to examine its effect on patient outcomes.

Bias↗

PubMed2000

Achieving change through reflective practice: closing the loop.

This paper draws on a small scale, exploratory study which was conducted in the clinical area in the early 1990s (Page 1992). The study drew on the principles of reflective practice in order to enable practitioners to identify learning needs in relation to cardiopulmonary resuscitation (CPR) procedures. Whilst considerable benefit from the reflective sessions was noted, one unanticipated and unpalatable outcome was what might be termed professional apathy. This was manifest in several ways, most importantly in that issues 'for action', identified through reflection, were not prioritised or acted upon. Whilst partial responsibility for this may lie with the somewhat naive way in which the reflective process was facilitated, some responsibility also rests with the nurses in question. One possible explanation is that, for changes in practice to occur, the planning and management of change should form an integral part of the reflective cycle. This point has been largely absent in the reflection literature to date. Such concerns are reviewed here in the context of nurse education's ongoing involvement with the notion of reflective practice and the role of the educationalist in the clinical area. The current political backdrop of 'quality initiatives' to create and demonstrate high standards of clinical care may be useful in advancing the debate.

Attitude of Health Personnel↗

PubMed1990

Auditory localization: effects of reflecting surfaces.

Based on anatomical and evolutionary conceptions of the human ear, an experiment was conducted in which forty-eight human subjects were asked to localize sounds (a human voice) emitted by one of twenty-seven stationary loudspeakers in an anechoic chamber. The position of the active loudspeaker varied with respect to azimuth, distance, and elevation in three steps each. The position of a single sound-reflecting surface (about 6 m2) was varied: on the floor, on the ceiling, to the left, and to the right. The accuracy of identifying the active loudspeaker for each position of the sound-reflecting surface was compared intraindividually with the absence of reflection. The results show an overall increase in correct localizations with a sound-reflecting surface on the floor. Especially the elevation of the sound source can be detected with greater precision. Additionally, the percentage of correct localizations decreased systematically with the presence of a sound-reflecting ceiling, while the presence of sound-reflecting walls did not systematically affect the localization performance. Judgments in the horizontal plane and those of distance were not systematically influenced by the presence of a sound-reflecting surface.

Adult↗

PubMed2003

Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves.

Leaf chlorophyll content provides valuable information about physiological status of plants. Reflectance measurement makes it possible to quickly and non-destructively assess, in situ, the chlorophyll content in leaves. Our objective was to investigate the spectral behavior of the relationship between reflectance and chlorophyll content and to develop a technique for non-destructive chlorophyll estimation in leaves with a wide range of pigment content and composition using reflectance in a few broad spectral bands. Spectral reflectance of maple, chestnut, wild vine and beech leaves in a wide range of pigment content and composition was investigated. It was shown that reciprocal reflectance (R lambda)-1 in the spectral range lambda from 520 to 550 nm and 695 to 705 nm related closely to the total chlorophyll content in leaves of all species. Subtraction of near infra-red reciprocal reflectance, (RNIR)-1, from (R lambda)-1 made index [(R lambda)(-1)-(RNIR)-1] linearly proportional to the total chlorophyll content in spectral ranges lambda from 525 to 555 nm and from 695 to 725 nm with coefficient of determination r2 > 0.94. To adjust for differences in leaf structure, the product of the latter index and NIR reflectance [(R lambda)(-1)-(RNIR)-1]*(RNIR) was used; this further increased the accuracy of the chlorophyll estimation in the range lambda from 520 to 585 nm and from 695 to 740 nm. Two independent data sets were used to validate the developed algorithms. The root mean square error of the chlorophyll prediction did not exceed 50 mumol/m2 in leaves with total chlorophyll ranged from 1 to 830 mumol/m2.

Algorithms↗

PubMed1999

Reflective properties of domestic fowl (Gallus g. domesticus), the fabric of their housing and the characteristics of the light environment in environmentally controlled poultry houses.

1. The light intensity and spectral power distribution in 3 broiler and 6 layer houses were sampled and converted to a measure which takes account of the domestic fowl's spectral sensitivity (termed lux corrected). Light intensity was highly variable around the layer houses (mean 33.2, s.d. 37.7 lux (corrected)) and to a lesser extent in the broiler houses (mean 5.2, s.d. 2.2 lux (corrected)). The mean intensities were also very much lower than on a sunny, June day at midday at Silsoe (42,937 lux (corrected)) and of different power distributions. 2. The reflectivity of the plumage of 15 traditional breeds of domestic fowl was surveyed. Most feathers showed a characteristic pattern of reflectivity, increasing either side of a wavelength of lambda = 400 nm. In 13 breeds the tail and wing feathers were darker than the breast and back feathers. Reflectivity at lambda = 700 nm was a good predictor of reflectivity at lambda = 320 nm. Food, bedding, wood and skin all had similar reflectivities to feathers. Metal, plastic and rubber all had more constant reflectivities across the spectrum. 3. The reflectivities of the fresh, feathered carcases of 3 male broilers (Cobb) and 3 layer hens (ISA Brown) were measured. There were small changes in hue and saturation within and between individual carcases, even for the apparently white broiler chickens. 4. Photographs were taken, with and without a UV(A)-only pass filter, of a broiler cock (Cobb), layer hen (ISA Brown) and cock jungle fowl. No additional patternation was evident in the UV(A)-only photographs and the jungle fowl lost most of its ornamentation. The texture of bare skin was enhanced in the UV(A)-only photographs. 5. The implications of these results for poultry behaviour are discussed.

Animals↗

PubMed1979

Quantitative reflection contrast microscopy of living cells.

Mammalian cells in culture (BHK-21, PtK2, Friend, human flia, and glioma cells) have been observed by reflection contrast microscopy. Images of cells photographed at two different wavelengths (546 and 436 nm) or at two different angles of incidence allowed discrimination between reflected light and light that was both reflected and modulated by interference. Interference is involved when a change in reflected intensity (relative to glass/medium background reflected intensity) occurs on changing either the illumination wavelength or the reflection incidence angle. In cases where interference occurs, refractive indices can be determined at points where the optical path difference is known, by solving the given interference equation. Where cells are at least 50 nm distant from the glass substrate, intensities are also influenced by that distance as well as by the light's angle of incidence and wavelength. The reflected intensity at the glass/medium interface is used as a standard in calculating the refractive index of the cortical cytoplasm. Refractive indices were found to be higher (1.38--1.40) at points of focal contact, where stress fibers terminate, than in areas of close contact (1.354--1.368). In areas of the cortical cytoplasm, between focal contacts, not adherent to the glass substrate, refractive indices between 1.353 and 1.368 were found. This was thought to result from a microfilamentous network within the cortical cytoplasm. Intimate attachment of cells to their substrate is assumed to be characterized by a lack of an intermediate layer of culture medium.

Animals↗

PubMed2003

Reflectance of Alaskan black spruce and white spruce foliage in relation to elevation and latitude.

Leaf reflectance at visible and near-infrared wavelengths (400-1000 nm) is related primarily to pigmentation, leaf structure and water content, and is an important tool for studying stress physiology and relationships between plants and their growth environment. We studied reflectance of two co-occurring Alaskan conifers, black spruce (Picea mariana (Mill.) BSP) and white spruce (Picea glauca (Moench) Voss), at elevations from 60 to 930 m a.s.l. along a latitudinal gradient from 61 degrees to 68 degrees N. Black spruce samples were collected from 24 sites and white spruce from 30 sites. Overall, reflectance spectra of the two species were similar, but from 400 to 700 nm, needle reflectance was consistently higher in black spruce than in white spruce (all P <or= 0.05). This difference is probably related to differences in epicuticular wax morphology or amount, and may represent a photoprotective mechanism in black spruce. Reflectance at visible wavelengths generally increased with elevation and latitude in both species, consistent with a general stress response. However, in a multiple regression, latitude and elevation explained only 25-45% of the total variation in the indices studied. Reflectance indices suggested that needle yellowness increased, whereas chlorophyll content and photochemical efficiency decreased with both elevation and latitude. These trends were consistent between species, but white spruce generally showed a much smaller (and insignificant) reflectance response to latitude compared with black spruce. Differences between species could be related to black spruce's ability to colonize more stressful sites and white spruce's greater competitiveness on less stressful sites, coupled with the effects of drainage and microtopography (which may vary less predictably with latitude than elevation) on species distribution. The black spruce results suggest that a 1000-m increase in elevation is roughly comparable with a 6 degrees increase in latitude.

Altitude↗

PubMed1995

Exit rounds: a reflection exercise.

This paper questions the long-held assumption that "experience is the best teacher". Reflection, it is argued, is the element that turns experience into learning. Unfortunately, reflection is the experiential learning skill in which student are most deficient. Further, clinical teachers may not appreciate the reciprocal and synergistic relationship of experience and reflection, and therefore they may not exploit its full potential. This paper argues for the formal and explicit use of reflection to clinical teaching. In addition to providing a theoretical rationale for the use of reflection is learning, one example of a reflection exercise--exit rounds--is described. Exit rounds focus on recently discharged patients and provide students with an opportunity to reflect on what they have learned from working with these patients. In addition to being a learning exercise, exit rounds provide attending physicians with an opportunity to address emotional issues, to evaluate students' learning and performances, to deal with "housekeeping" details, and to provide closure.

Clinical Competence↗

PubMed2000

An optical model of the human retinal nerve fiber layer: implications of directional reflectance for variability of clinical measurements.

PURPOSE: The reflectance of the retinal nerve fiber layer is highly directional--that is, it depends strongly on the angles of illumination and viewing. This study explored and illustrated the implications of this directional reflectance for nerve fiber layer measurements in the human eye. METHODS: The retina was modeled as a sphere centered on the optic axis of a schematic eye. Nerve fiber ribbons were projected onto the retina and cylindrical light scattering was calculated along each ribbon. The reflectance along the ribbon was then determined for the illuminating and viewing apertures of two hypothetical optical instruments, a fundus camera and a confocal scanning laser ophthalmoscope. Results were displayed as reflectance maps. RESULTS: Uniformly illuminated nerve fiber ribbons exhibited a nonuniform reflectance pattern that was very sensitive to the location in the pupil of the instrument apertures. Ribbon reflectance at the superior and inferior disc margins varied with ribbon orientation, being higher with temporal tilt and lower with nasal tilt. Ribbons nasal to the disk could be quite dim. CONCLUSIONS: In quantitative nerve fiber layer assessment technologies, the observed reflectance depends on the configuration of the illuminating and viewing apertures of the measuring instrument and on the retinal position and orientation of each nerve fiber bundle. In clinical practice, this dependence may cause significant measurement variability that can be reduced by specific measurement maneuvers.

Fundus Oculi↗