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Unsteady forces and flows in low Reynolds number hovering flight: two-dimensional computations vs robotic wing experiments.

We compare computational, experimental and quasi-steady forces in a generic hovering wing undergoing sinusoidal motion along a horizontal stroke plane. In particular, we investigate unsteady effects and compare two-dimensional (2D) computations and three-dimensional (3D) experiments in several qualitatively different kinematic patterns. In all cases, the computed drag compares well with the experiments. The computed lift agrees in the cases in which the sinusoidal changes in angle of attack are symmetrical or advanced with respect to stroke positions, but lags behind the measured 3D lift in the delayed case. In the range of amplitudes studied here, 3-5 chords, the force coefficients have a weak dependence on stroke amplitude. As expected, the forces are sensitive to the phase between stroke angle and angle of attack, a result that can be explained by the orientation of the wing at reversal. This dependence on amplitude and phase suggests a simple maneuver strategy that could be used by a flapping wing device. In all cases the unsteady forces quickly reach an almost periodic state with continuous flapping. The fluid forces are dominated by the pressure contribution. The force component directly proportional to the linear acceleration is smaller by a factor proportional to the ratio of wing thickness and stroke amplitude; its net contribution is zero in hovering. The ratio of wing inertia and fluid force is proportional to the product of the ratio of wing and fluid density and the ratio of wing thickness and stroke amplitude; it is negligible in the robotic wing experiment, but need not be in insect flight. To identify unsteady effects associated with wing acceleration, and coupling between rotation and translation, as well as wake capture, we examine the difference between the unsteady forces and the estimates based on translational velocities, and compare them against the estimate of the coupling between rotation and translation, which have simple analytic forms for sinusoidal motions. The agreement and disagreement between the computed forces and experiments offer further insight into when the 3D effects are important. A main difference between a 3D revolving wing and a 2D translating wing is the absence of vortex shedding by a revolving wing over a distance much longer than the typical stroke length of insects. No doubt such a difference in shedding dynamics is responsible in part for the differences in steady state force coefficients measured in 2D and 3D. On the other hand, it is unclear whether such differences would have a significant effect on transient force coefficients before the onset of shedding. While the 2D steady state force coefficients underpredict 3D forces, the transient 2D forces measured prior to shedding are much closer to the 3D forces. In the cases studied here, the chord is moving between 3 to 5 chords, typical of hovering insect stroke length, and the flow does not appear to separate during each stroke in the cases of advanced and symmetrical rotation. In these cases, the wing reverses before the leading edge vortex would have time to separate even in 2D. This suggests that the time scale for flow separation in these strokes is dictated by the flapping frequency, which is dimensionally independent. In such cases, the 2D unsteady forces turn out to be good approximations of 3D experiments.

Animals↗

The basic diagnostic approaches used in robotic still-image telepathology.

We investigated the basic diagnostic processes used in telepathology with our robotic still-image system, OLMICOS, by analysing the steps and patterns used in 20 consecutive tissue section diagnoses. Three basic approaches were recognized. One was magnifying a suspect finding in a low-powered microscopic image. This approach was used mostly for confirming or characterizing a tumour. The second approach was scanning over a low-powered image by magnifying square images to form a mesh. This was found to be useful to confirm the presence or absence of signs and was mostly used as the initial step in judging the surgical margin of malignant cases or diagnosing lesions. The third approach was a combination of these two and was used for delineating the surgical margin, confirming the absence of metastases or diagnosing difficult lesions. Recognition of these three basic diagnostic approaches is important in making a rapid and correct remote diagnosis.

Humans↗

Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer.

Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.

Automation↗

A robot-controlled microwave antenna system for uniform hyperthermia treatment of superficial tumours with arbitrary shape.

A system for microwave hyperthermia by scanning an antenna 'footprint' over a disease site is demonstrated. A computer-controlled robot arm scans a 2.45 GHz helical antenna and controls the absorbed power distribution. The 'ideal' power distribution required to achieve steady-state temperature uniformity over a given region has been calculated and a corresponding antenna trajectory programmed to approximate this pattern. Computer models, based on the solution of the bio-heat equation, have been implemented to allow various system parameters, such as antenna beam size, scan path and velocity profile, to be optimized. Experiments on a homogeneous muscle-equivalent phantom have shown that the scanning antenna system produces uniform temperature distributions over large areas of arbitrary shape. The addition of a thermal control system, based on temperature signal feedback, would enable 'real-time' modification of the power distribution and allow inhomogeneous and perfused tissue structures to be heated more uniformly.

Computer Simulation↗

A robotic DNA purification protocol and real-time PCR for the detection of Campylobacter jejuni in foods.

Primers designed to amplify a Campylobacter jejuni cadF gene sequence were used in an SYBR Green I real-time PCR assay as an alternative to conventional bacteriological methods for the rapid detection of C. jejuni in foods. Twenty-five grams of chicken skin (breast and thigh) was contaminated by adding approximately 1, 10, or 50 CFU of C. jejuni ATCC 35560. Twenty-five grams of pork and 25-ml aliquots of milk were also inoculated with 1 and 10 CFU of the pathogen. The samples were incubated in Bolton broth for different periods at 37 and 42 degrees C under microaerophilic conditions. Using a commercial robotic DNA purification system, DNA was extracted and purified from 1-ml aliquots of the enrichment cultures before and after centrifugation of the 250-ml enrichment broth at 15,900 x g for 10 min at 4 degrees C. The DNA was used as the template in a real-time PCR assay. C. jejuni was detected after 12 h of enrichment from samples inoculated with about 50 CFU/25-g sample. After centrifugation, an enrichment step of 8 h was sufficient to allow detection of pathogen in samples inoculated with 10 CFU/25 g. However, 24 h of enrichment was necessary to detect pathogen in samples inoculated with approximately 1 CFU/25 g. The real-time PCR protocol developed in this study significantly reduced the detection time of C. jejuni in foods.

Animals↗

Challenges and approaches to the robotic detection of enantioenrichment on Saturn's moon, Titan.

Saturn's moon, Titan, is one of the most interesting locations for organic chemistry in the solar system. As a possible follow up to the Cassini-Huygens mission, which will conduct the first in situ analysis of the Titan atmosphere in 2004, studies are underway for missions to explore in detail the chemical composition of Titan's atmosphere, surface, and oceans. In order to seek out and explore complex chemical systems that may represent steps on the pathway to life, instruments for detection of enantioenrichment should be included on such a mission. The formidable challenges of robotic measurement of enantioenrichment on Titan are summarized, and experimental approaches to this problem are reviewed. In addition, some speculations are offered concerning locations on Titan where complex fractionation of organic materials may have occurred.

Journal Article↗

A mutation in Af4 is predicted to cause cerebellar ataxia and cataracts in the robotic mouse.

The robotic mouse is an autosomal dominant mutant that arose from a large-scale chemical mutagenesis program. It has a jerky, ataxic gait and develops adult-onset Purkinje cell loss in the cerebellum in a striking region-specific pattern, as well as cataracts. Genetic and physical mapping of the disease locus led to the identification of a missense mutation in a highly conserved region of Af4, a putative transcription factor that has been previously implicated in leukemogenesis. We demonstrate that Af4 is specifically expressed in Purkinje cells, and we hypothesize that the expression of mutant Af4 leads to neurodegeneration. This function was not identified through knock-out studies, highlighting the power of phenotype-driven mutagenesis in the mouse to identify new pathways involved in neurological disease.

Amino Acid Sequence↗

Conversion of Endoscopic, Robotically Assisted Coronary Bypass: Incidence, Risk Factors, and Outcome.

Abstract Background: The clinical and financial consequences of conversion from endoscopic (robotically assisted) atraumatic coronary artery bypass (EndoACAB) to sternotomy (converted EndoACAB) have not been previously reported. This study sought to identify the incidence, causes, predictive factors, and adverse consequences of converted EndoACAB. Methods: Between June 1996 and June 2003, 509 patients underwent EndoACAB, and an additional 20 EndoACAB patients underwent converted EndoACAB. Data from the patients requiring conversion to sternotomy were retrospectively reviewed using multivariate regression and computer matched with data from a cohort of patients who underwent primary sternotomy (SternCAB). Results: The overall rate of conversion was 3.8% (20/509). Causes were: inability to expose the target vessel(s) (9), unsuitable internal mammary artery (7), intrathoracic bleeding (2), and hemodynamic instability (2). There were no statistical differences in mortality or major morbidity between converted EndoACAB patients versus the computer-matched SternCAB patients ( P = not significant). Hospital costs for the converted EndoACAB patients were higher than for the successful EndoACAB patients, but not higher than the computer-matched SternCAB patients. Conclusions: The incidence of conversion of EndoACAB patients to sternotomy was low and often occurred under elective circumstances. The clinical and economic consequences of conversion were minimal.

Journal Article↗

[Intra-operative stereotactic accuracy of computer-assisted robot orthopaedic trauma surgery planning system].

OBJECTIVE: To investigate the feasibility and accuracy of the software computer-assisted robot orthopaedic trauma surgery planning system (CAOTS). METHODS: The software CAOTS was developed and used on 85 cases of stereotactic operation, 24 model bones, 21 cadaveric extremity bones, and 40 patients. 307 groups of directional targets in these cases were randomly sampled. The distribution of error sources for evaluating system performance was embodied using Monte-Carlo method in order to derive the theoretic guarantees for further optimizing and enhancing the system performance, then the software SPSS 2.0 was used to analyze the errors. RESULTS: The statistical area of deviation number was 0.0408 +/- 0.4578 mm, corresponding to the result by Monte-Carlo method. Punching succeeded at the first run for all 307 cases without wrong locking and other clinical complications. CONCLUSION: Accurate and reliable, CAOTS improves the intra-operative navigation techniques and facilitates the orthopedists to perform operation.

Cadaver↗

Robotic ureterolysis, retroperitoneal biopsy, and omental wrap for the treatment of ureteral obstruction due to idiopathic retroperitoneal fibrosis.

Retroperitoneal fibrosis (RPF) is a rare disorder of unclear etiology characterized by chronic inflammation of the retroperitoneum, which can involve any of the retroperitoneal structures, most notably the ureters, aorta, and vena cava. Historically, open biopsy, ureterolysis, and transpositioning or omental wrapping of the involved ureter(s) have been the preferred surgical treatments of RPF, with success rates greater than 90%. More recently, successful laparoscopic biopsy, ureterolysis, and ureteral omental wrapping and intraperi-tonealization have been described. We report the first case in the literature of idiopathic RPF managed with robotic ureterolysis and laparoscopic omental ureteral wrapping.

Case Reports↗

Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.

This paper presents the first automated system for simultaneously detecting human papilloma, herpes simplex, adenovirus, or cytomegalovirus viral antigens and gene sequences in standard formalin-fixed, paraffin-embedded tissue substrates and tissue culture. These viruses can be detected by colorimetric in situ nucleic acid hybridization, using biotinylated DNA probes, or by indirect immunoperoxidase techniques, using polyclonal or monoclonal antibodies, in a 2.0-hour assay performed at a single automated robotic workstation.

Antigens, Viral↗

A robotic system to prepare samples for HTLV-III testing.

A robotic handling system was adapted to perform the sampling and dilution steps needed in an assay to detect antibodies to the HTLV-III virus, the causative agent of AIDS. The system reduced the labor required to prepare the samples and provided standardization and accuracy in the preparation of the samples.

Antibodies, Viral↗

Evaluation of robot automated chromogenic substrate LAL endotoxin assay method for pharmaceutical products testing.

The robot automated chromogenic substrate LAL assay method was evaluated for endotoxin testing using three lots each of 12 pharmaceutical products. As many as 216 assays, including automated standard curve construction and sample preparation, can be performed in a single day of unattended operation. The method is linear (r greater than .99) in the range of 0 to 0.2 EU/ml. The precision of the method determined by assaying a lot of calcium gluconate for four days was 6%, 10%, and 10% for within an assay block, between assay blocks, and between assay days, respectively. Recovery of endotoxin when spiked into products ranged from 81% to 110% and was within the statistical variation (2 sigma limit) of the method. The endotoxin levels detected in a biological raw material by the chromogenic substrate assay method correlated well with that of the gel-clot LAL assay method. The endotoxin content of the majority of the pharmaceutical products tested was well below the sensitivity of both the chromogenic substrate and the gel clot LAL assay methods.

Automation↗

[From automation to robotics].

The introduction of automation into the laboratory of biology seems to be unavoidable. But at which cost, if it is necessary to purchase a new machine for every new application? Fortunately the same image processing techniques, belonging to a theoretic framework called Mathematical Morphology, may be used in visual inspection tasks, both in car industry and in the biology lab. Since the market for industrial robotics applications is much higher than the market of biomedical applications, the price of image processing devices drops, and becomes sometimes less than the price of a complete microscope equipment. The power of the image processing methods of Mathematical Morphology will be illustrated by various examples, as automatic silver grain counting in autoradiography, determination of HLA genotype, electrophoretic gels analysis, automatic screening of cervical smears... Thus several heterogeneous applications may share the same image processing device, provided there is a separate and devoted work station for each of them.

Automation↗

Automated DNA purification and amplification from blood-stained cards using a robotic workstation.

A new method for the purification of DNA on blood-stained cards was developed. This method was implemented into a high-throughput automated system using a Biomek 1000 robotic workstation. In addition, the processes of DNA purification and amplification were coupled into a completely automated and uninterrupted prototype system, and the resultant PCR products generated by this system were subjected to automated desalting for capillary electrophoresis analysis.

Autoanalysis↗

The development of the Braun Oral-B Ultra Plaque Remover: an in vitro robot study.

PURPOSE: To assess the efficacy of a new brush head design and brushing action using a novel robot system which simulates normal clinical toothbrush use. The system allows developments in toothbrush design to be assessed prior to embarking on expensive and time consuming clinical trials. MATERIALS AND METHODS: Using this system, the efficacy of a new brush head design combined with a modified brushing speed, was compared with the clinically established Braun Oral-B Plaque Remover (D7 handle and EB5 brush head). RESULTS: A combination of a new brush head (EB9) with longer tufts of bristles designed to improve interdental penetration, and an increased frequency of bristle movement (63Hz, 3,800 strokes per minute - D9 handle) was associated with significantly greater removal of artificial plaque. Visual observation of typodonts suggested that this increased efficacy is related to greater removal of plaque from interdental surfaces and occlusal fissures. These results suggest that the D9/EB9 prototype electric toothbrush is suitable for further investigation and clinical evaluation.

Dental Plaque↗

Robotics and allied technologies in endoscopic surgery.

Endoscopic surgery was developed in the 1970s and 1980s, with initial work conducted by pioneering surgeons. After the development of laparoscopic cholecystectomy, the breakthrough of endoscopic surgery had a great effect on all surgical specialties. Starting with rather simple procedures, such as cholecystectomy, a rapid progression toward more complex procedures, such as reflux or colonic surgery, took place. It was realized at this time that the existing endoscopic instruments allowed only a limited preciseness when performing the procedures, and part of the information from inside the abdominal cavity was not available to the surgeon. This prompted a discussion with engineers concerning the development of more advanced technologies to give those performing endoscopic surgery the same quality of information and manipulation that surgeons have when performing open surgery. These qualities include (1) instruments and manipulators that allow surgical action under endoscopic control with all degrees of freedom; (2) devices that provide surgeons with tactile feedback; and (3) vision systems that provide surgeons with the same quality of visual information as with open surgery, namely, high resolution, excellent color quality, precise spatial information, and a constant clear view for optimal surgical action. At the end of 1999, some of the aforementioned quality concepts found their way into the surgical routine, but most of the concepts are still being developed. Another decade will pass before endoscopic surgery procedures will be closer to the technological goals.

Endoscopy↗