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At least 19 recordsLinked to original sources

Immunohistochemical localization of immunoglobulins in bovine granulomatous lesions.

The immunohistochemical distribution of IgG, IgA and IgM in granulomatous lesions caused by Actinomyces bovis, Actinobacillus lignieresi, Actinomyces (Corynebacterium) pyogenes, Pseudomonas aeruginosa, Staphylococcus aureus and Mycobacterium bovis was studied. Numerous IgG-containing cells (plasma cells) were distributed in the peripheral connective tissue layers, but not in the epithelioid cell layer. A few scattered IgA- and IgM-containing cells were found in all the lesions examined. Mycobacterial granulomas contained fewer IgG-cells than did non-mycobacterial granulomas. Eosinophilic club-shaped bodies were found in A. bovis, A. lignieresi, P. aeruginosa and S. aureus, but they were generally negative for IgG, IgA and IgM.

Actinobacillus Infections↗

Immunohistochemical distribution of S-100 alpha-positive cells in bovine mycobacterial and non-mycobacterial granulomas.

By means of immunohistochemistry, the distribution of the alpha-subunit (S-100 alpha) and the beta-subunit (S-100 beta) of S-100 protein was studied in bovine granulomas caused by Actinomyces bovis, Actinobacillus lignieresi, Actinomyces (Corynebacterium) pyogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Mycobacterium bovis and Mycobacterium paratuberculosis. S-100 alpha-positive epithelioid cells or dendritic cells were scattered among the predominantly S-100 alpha-negative cells of the mononuclear phagocyte system (MPS). S-100 beta was not found in the MPS cells of granulomas but was observed in the endothelial cells of blood vessels. A positive reaction to S-100 was also seen in normal cells in the lymphoid and mammary tissues. Mycobacterial granulomas contained more S-100 alpha-positive cells than did non-mycobacterial ones.

Animals↗

A comparison of various Haemophilus somnus strains.

Sixty-eight Haemophilus somnus strains isolated from the bovine in Canada and the U.S.A. were compared. In media enriched with 5% ovine serum, 5% bovine serum and 10% yeast extract, H. somnus fermented glucose, levulose, maltose, mannitol, mannose, sorbitol, trehalose and xylose, but failed to ferment arabinose, dulcitol, galactose, inositol, lactose, raffinose, rhamnose, salicin and sucrose. The organisms acidified litmus milk, produced cytochrome oxidase, indole and hydrogen sulfide (H(2)S) and reduced nitrates to nitrites. The motility, methyl-red, acetylmethyl-carbinol urease catalase, citrate, malonate, lysine, ornithine and arginine tests were negative. Haemophilus somnus was resistant to lincomycin, neomycin and triple sulfa, but susceptible to ampicillin, chloramphenicol, streptomycin, penicillin and tetracycline. No antigenic differences were noted between strains when tested against rabbit antisera of eight strains using agglutination, complement-fixation, immunodiffusion and counterimmunoelectrophoresis tests. Low titre cross-reactions were found in the agglutination tests with some of the anti-H. somnus rabbit sera with Actinobacillus lignieresi and Moraxella bovis. No distinct antigenic similarities to nine other species of pathogenic bacteria of animal origin were found. No difference was observed between H. somnus isolates from Ontario and those from western Canada and the U.S.A.

Agglutination↗

An outbreak of diphtheria in Boergoat kids.

During an outbreak of diphtheria or ulcerative stomatitis in young Boergoat kids Fusobacterium necrophorum was consistently isolated under anaerobic cultural conditions from material taken from the edges of the ulcerative lesions from all of the cases examined. Corynebacterium pyogenes, Staphylococcus aureus, Actinobacillus lignieresi, Moraxella spp. and Lactobacillus spp. were also isolated anaerobically and/or aerobically. The typical symptoms and lesions were confirmed to the mouth, tongue and throat regions.

Animals↗