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At least 19 recordsLinked to original sources
PubMed1974

Evaluation fo membrane filter field monitors for microbiological air sampling.

Due to area constraints encountered in assembly and testing areas of spacecraft, the membrane filter field monitor (MF) and the National Aeronautics and Space Administration-accepted Reyniers slit air sampler were compared for recovery of airborne microbial contamination. The intramural air in a microbiological laboratory area and a clean room environment used for the assembly and testing of the Apollo spacecraft was studied. A significantly higher number of microorganisms was recovered by the Reyniers sampler. A high degree of consistency between the two sampling methods was shown by a regression analysis, with a correlation coefficient of 0.93. The MF samplers detected 79% of the concentration measured by the Reyniers slit samplers. The types of microorganisms identified from both sampling methods were similar. Variables in the MF samplers, such as pore size, relative humidity, and flow rates, have been studied, but no effect was noted on recovery. The results show that the MF method could be used to estimate the number and types of microorganisms found in the air.

Actinomycetales↗
PubMed2004

Comparison of the collecting efficiency of microbiological air samplers.

It is important to evaluate the prevention of nosocomial infections caused by airborne micro-organisms in healthcare facilities. For that purpose, the use of an air sampler is required. A comparison of the collecting efficiency of air samplers has not been studied so far using a single culture medium. Use of the same culture medium is necessary because of the variation of the cultivation efficiency with different culture media. In this study a statistically significant difference in collecting efficiency among air samplers was observed in case of SCDA (soybean casein digest agar) culture medium, but not SCDALP (soybean casein digest agar lecithin polysorbate) culture medium. In addition, a statistically significant difference of the cultivation efficiency was observed between SCDA and SCDALP culture media (P<0.05). These results suggest that any air sampler can be used with SCDALP culture medium.

Air Microbiology↗
PubMed1997

[Air microbiology monitoring in plenum-ventilated operating rooms. Proposed standards].

BACKGROUND: Microbiological standard for air in plenum ventilated operating theatres are not defined. The aim of this study was to analyze the microbiological contamination of air in HUSC theatres and to establish standards of reference. METHODS: 408 air samples (80 l/sample) were taken from air intake and center of the theatres in three surgical areas, throughout five consecutive years (1991-1995). RCS air sampler was used. Media of microbiological counts were compared using the ANOVA and the Kruskall-Wallis tests. Microbiological standards were established based on upper limit of confidence interval of counts, when the degree of contamination was similar in two consecutive years. RESULTS: A falling trend of microbiological counts was observed, with statistical significance between counts found in 1992 and those found in 1994 and 1995 (p < 0.01). There was no difference between results of the three surgical areas, but there was a significant difference between counts from air intake and center of theatres, regardless the year or the surgical area (p < 0.01). During this time a significant decrease in the percentage of samples with presence of fungi (p = 0.001) was produced. The results of years 1994 and 1995 were taken for establish the microbiological standards. CONCLUSIONS: This control program allowed to evaluate and improve the hygienic conditions in the operating theatres and to establish a microbiological standards of reference for plenum ventilated theatres.

Air Microbiology↗
PubMed1996

An assessment of the Sartorius MD8 microbiological air sampler.

Tests described in this paper show that gelatine membrane filters used in the MD8 microbiological air sampling system collected monodispersed aerosols between 0.7 and 1.0 microns containing viable Bacillus subtilis var. niger spores, with an efficiency of 99.9995%. Gelatine membrane filters linked to the MD8 control pump system were as effective as the well established Casella slit-to-agar device for collecting some viable bacteria, nebulized under controlled experimental conditions and naturally occurring airborne micro-organisms in a pharmaceutical plant. By using a long flexible hose connection to the control pump, the head could be positioned where sampling was required in locations remote from the pump exhaust, making it suitable for microbiological monitoring in critical locations such as laminar flow stations and isolators.

Aerosols↗
PubMed2005

Indoor air microbiological evaluation of offices, hospitals, industries, and shopping centers.

In this study it was compared the MAS-100 and the Andersen air samplers' performances and a similar trend in both instruments was observed. It was also evaluated the microbial contamination levels in 3060 samples of offices, hospitals, industries, and shopping centers, in the period of 1998 to 2002, in Rio de Janeiro city. Considering each environment, 94.3 to 99.4% of the samples were the allowed limit in Brazil (750 CFU/m3). The industries' results showed more important similarity among fungi and total heterotrophs distributions, with the majority of the results between zero and 100 CFU/m3. The offices' results showed dispersion around 300 CFU/m3. The hospitals' results presented the same trend, with an average of 200 CFU/m3. Shopping centers' environments showed an average of 300 CFU/m3 for fungi, but presented a larger dispersion pattern for the total heterotrophs, with the highest average (1000 CFU/m3). It was also investigated the correlation of the sampling period with the number of airborne microorganisms and with the environmental parameters (temperature and air humidity) through the principal components analysis. All indoor air samples distributions were very similar. The temperature and air humidity had no significant influence on the samples dispersion patterns.

Air Microbiology↗