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At least 19 recordsLinked to original sources
PubMed2003

Highly sensitive amplified electronic detection of DNA by biocatalyzed precipitation of an insoluble product onto electrodes.

The amplified detection of a target DNA, based on the alkaline phosphatase oxidative hydrolysis of the soluble 5-bromo-4-chloro-3-indoyl phosphate to the insoluble indigo product as an amplification path, is addressed by two different sensing configurations. The accumulation of the insoluble product on Au electrodes or Au/quartz crystals alters the interfacial electron-transfer resistance at the Au electrode or the mass associated with the piezoelectric crystal, thus enabling the quantitative transduction of the DNA sensing by Faradaic impedance spectroscopy or microgravimetric quartz crystal microbalance measurements, respectively. One sensing configuration involves the association of a complex consisting of the target DNA and a biotinylated oligonucleotide to the functionalized transducers. The binding of the avidin/alkaline phosphatase conjugate to the sensing interface followed by the biocatalyzed precipitation provides the amplification path for the analysis of the target DNA. This analysis scheme was used to sense the target DNA with a sensitivity limit that corresponds to 5 x 10(-14) M. The second amplified detection scheme involves the use of a nucleic-acid-functionalized alkaline phosphatase as a biocatalytic conjugate for the precipitation of the insoluble product. Following this scheme, the functionalized transducers are interacted with the analyzed sample that was pretreated with the oligonucleotide-modified alkaline phosphatase, followed by the biocatalyzed precipitation as the amplification route for the analysis of the target DNA. By the use of this configuration, a detection limit corresponding to 5 x 10(-13) M was achieved. Real clinical samples of the Tay-Sachs genetic disorder were easily analyzed by the developed detection routes.

Alkaline Phosphatase↗
PubMedDate not supplied

[Comparison of auscultation findings using a classic stethoscope (Litmann 2120) and electronically amplified stethoscope (Medmax2)].

Heart auscultation has one of the key roles in beside diagnosis, especially in patients with cardiovascular diseases. Sometimes, because of the human cars' low sensitivity, a problem emerges in the proper evaluation of heart sounds and murmurs of lower frequencies. Our study compared two stethoscopes, the classic acoustic stethoscope (Littmann 2120) and an electronic one with the sound amplifier and the noise filtering system (Medmax2) in 10 patients examined by 10 physicians. Significantly better detection of low frequency sounds was found in favour of electronic stethoscope (chi 2 = 17.9; p < 0.0001). It is concluded that the selective amplificator improves the stethoscope performance and has its place in everyday bedside practice, especially in departments of cardiology.

Aged↗
PubMed2001

Detection of single-base DNA mutations by enzyme-amplified electronic transduction.

Here we describe a method for the sensitive detection of a single-base mutation in DNA. We assembled a primer thiolated oligonucleotide, complementary to the target DNA as far as one base before the mutation site, on an electrode or a gold-quartz piezoelectric crystal. After hybridizing the target DNA, normal or mutant, with the sensing oligonucleotide, the resulting assembly is reacted with the biotinylated nucleotide, complementary to the mutation site, in the presence of polymerase. The labeled nucleotide is coupled only to the double-stranded assembly that includes the mutant site. Subsequent binding of avidin-alkaline phosphatase to the assembly, and the biocatalyzed precipitation of an insoluble product on the transducer, provides a means to confirm and amplify detection of the mutant. Faradaic impedance spectroscopy and microgravimetric quartz-crystal microbalance analyses were employed for electronic detection of single-base mutants. The lower limit of sensitivity for the detection of the mutant DNA is 1 x 10-14 mol/ml. We applied the method for the analysis of polymorphic blood samples that include the Tay-Sachs genetic disorder. The sensitivity of the method enables the quantitative analysis of the mutant with no PCR pre-amplification.

Alkaline Phosphatase↗
PubMed2002

Is Electronically Amplified Music too Loud? What do Young People Think?

Listening to loud music has been associated, in a number of studies, with hearing loss and tinnitus among young people. However an unanswered question is whether or not these same young people want to have their music so loud. In our study 533 young men and 167 young women, in the age group 16 to 25, who were attending a vocational training centre, responded to a questionnaire and volunteered to have their hearing assessed. The questionnaire sought information on listening habits, on the kinds of events attended, on whether the music at these events was too loud or not, and if the respondents considered their hearing had been impaired. Analysis of this data indicated that 79% of the subjects attend discotheques, 52% pop and rock concerts, and 35% techno parties (e.g. raves). A significant number considered the music at these venues was too loud. Some 42% considered this was the case at discos, 35% thought pop and rock concerts too loud, and 39% held a similar view of techno parties. Conversely, fewer than 3% considered sound levels at these events to be too low. On the basis of the response to the questionnaire we estimate that over half the respondents (56.6%) have a sound exposure (L(eq)) from music of over 87 dB(A). It is not surprising therefore that 71% reported that they had suffered tinnitus following attendance at a music event. The hearing capacity of the sample was measured by audiometry. These measurements detected hearing loss in 11% of the 700 individuals tested. However it was not possible to show that the risk of hearing loss increased with increasing exposure to loud music. We conclude that young people neither demand nor require the excessive sound levels typical of most music events.

Journal Article↗