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[Calcitonin in the ultimobrancial body of Anguilla (Anguilla anguilla L.): cytologic localization by indirect immunofluorescence using human anti-salmon-calcitonin antibodies].

The localization of intracellular calcitonin has been achieved by immunofluorescence in the cytoplasm of all cells forming the epithelium of the ultimobranchial body of eels, using a human antiserum against synthetic Salmon calcitonin I. The specificity of the reaction is demonstrated by inhibition with synthetic salmon calcitonin (S.C.T.); the fluorescence is not inhibited by synthetic human calcitonin (H.C.T.).

Anguilla

[Studies on blood morphology in the European eel (Anguilla anguilla). I. Erythrocytes and their developmental stages].

By means of heart tapping we produced a larger loss of blood in the European eel, Anguilla anguilla. This resulted in enriching the peripheric blood with erythroblasts and proerythrocytes. By panoptic staining erythroblasts, proerythrocytes and mature erythrocytes could be prepared and described. By the aid of supravital staining it was possible to detect mitochondria and Substantia granulofilamentosa in all stages of development. Phase contrast observations proved that the erythrocytes of Anguilla anguilla do not possess any locomotion ability. Cytochemical investigations showed that the erythrocytes weakly or very weakly react to acid phosphatases, lipids and ribonucleic acids and positively to unspecific esterases. The artificially produced loss of blood and the following continuous extraction of blood gave indications of how rapidly the erythrocytes form and how much time their maturing takes. 48 hours after tapping erythroblasts appeared in large amounts. The number of proerythrocytes increased 24 hours later. The transformation of proerythrocytes into mature erythrocytes does not take more than 48 hours. The loss of erythrocytes caused by heart tapping is already compensated six days later by new growth. During this investigation division processes in erythrocytes were observed. These are endomitotic or amitotic division or mitotic ones which are very rarely observed.

Acid Phosphatase

Effect of deterlon (DBS) on the electrocardiogram of eel Anguilla anguilla (L).

Effect of Deterlon (DBS) on the electrocardiogram of eel Anguilla anguilla (L). Acta Physiol. Pol. 1977, 28 (2): 179--186. The investigations of the effect of a detergent Deterlon-DBS present in industrial and community sewage on ECG changes in the eel Anguilla anguilla (L) showed that it causes disturbances in the impulse-generating centres and in the mechanism of impulse conduction in the heart. It was found that the threshold concentration of Deterlon-DBS in aqueous medium is 3 mg SA/1 for the eel, the lethal concentration lying in the range of 60--100 mg SA/1.

Animals

Formaldehyde-induced fluorescence in the telencephalon and diencephalon of the eel (Anguilla anguilla l.). A fluorescence-microscopic and microspectrofluorometric investigation with special reference to the innervation of the pituitary.

In the telencephalon and diencephalon of the eel (Anguilla anguilla L.) formaldehyde-induced fluorescence was studied microscopically and microfluorometrically with special emphasis on the innervation of the pituitary. In the telencephalon fluorescent fibers contained predominantly noradrenaline fluorophores. Fluorescent nuclei could not be established. In the diencephalon fluorescent perikarya were found in: (1) the paraventricular organ (PVO), possessing either dopamine or, to a lesser extent, serotonin fluorophores; (2) the PVO-accompanying group, exhibiting spectral data resembling those of noradrenaline fluorophores; (3) the nucleus hypothalami anterior (NHA), a small paired group of catecholamine-containing cells posterior to the commissura transversa.--The nucleus lobi inferioris exhibited a high density of delicate, most probably dopamine-containing terminals, while fibers surrounding this nucleus contained noradrenaline fluorophores. A high density of fluorescent terminals containing dopamine and/or noradrenaline was found in the habenular complex. Fluorescent terminals in the pituitary contained fluorophores resembling either dopamine or noradrenaline. Fluorescent tracts entered the pituitary from different directions. A rostral, unpaired tract enters the neurointermediate lobe, as also verified experimentally. The rostral pars distalis receives two paired tracts, one from a rostral and one from a dorsal direction. The proximal pars distalis also receives two paired tracts, one from a dorsal and one from a posterior direction.

Anguilla

Responses of the ultimobranchial body in eels (Anguilla anguilla L.) maintained in sea water and experimentally matured, to injections of synthetic salmon calcitonin.

The effect of a synthetic salmon calcitonin (SCT) treatment on ultimobranchial body (UB) activity in eels (Anguilla anguilla L.) maintained in seawater and submitted to experimental maturation, has been studied histologically. 2. The activity of the glands of a control group of eels maintained in sea water was taken as a reference. 3. The UB parenchyma showed a marked atrophy in the fish treated with SCT alone and serum calcium decreased significantly in this group. 4. Immature female silver eels receiving carp pituitary extract (CPE 1 mg/100 g body wt. per injections) until complete maturation presented high hypercalcemia associated with cellular hypertrophy and hyperplasia in the UB. 5. SCT treatment did not prevent the hypercalcemia provoked by CPE injections. UB activity was strongly increased in this case. 6. These data indicate that the activity of the UB in eels varies with both physiological and experimental hypercalcemia, and responds to SCT injections.

Anguilla

Catecholamine fluorescence in the pituitary of the eel, Anguilla anguilla, with special reference to its variation during background adaptation.

In the neuro-intermediate lobe (NIL) of the eel, Anguilla anguilla, a specific formaldehyde-induced fluorescence, indicating a catecholamine (CA) innervation, has been demonstrated in the neural lobe processes. Microspectrofluorimetric analyses and pharmacological treatments indicate noradrenaline or dopamine or both to be responsible for the fluorescence. The fluorescence in the NIL has displayed a definite tendency toward variation during the adaptation to a white and to a black background. The highest amounts of fluorescence were generally found in animals adapted to a black background, especially when adapted for a rather long period, and in animals recently transferred to a white background. The lowest amounts of fluorescence were generally found in animals adapted to a white background. This and the result of injections of CA-depleting drugs suggest that the monoaminergic nerves are active when the animal is on a white background, inhibiting the MSH release directly or indirectly or both, or in co-operation with other factors. Specific green fluorescent structures were also found in other parts of the neural lobe supplying the pars distalis. In some pharmacologically untreated specimens and in animals treated with CA-depleting drugs, the intermedia cells fluoresced. Microspectrofluorimetric analyses indicated that this fluorophore was not a CA.

Adaptation, Physiological

The effects of transecting the IXth and Xth cranial nerves on hydromineral balance in the eel Anguilla anguilla.

The IXth and the Xth cranial nerves in Anguilla anguilla were transected, and the effects upon ion and water balance were studied in fresh water and sea water, and during transfer from fresh water and vice versa. In fresh water there is a slow demineralization due to an excess loss of Na and Cl ions. During freshwater to seawater transfer the eel survives only for 4-5 days. The fish do not drink and Na efflux does not increase enough to extrude excess ions. In sea water the glossopharyngeal and vagus nerves are necessary for the maintenance of the hydromineral balance. Denervation is followed by an increase in plasma ion concentrations. Na fluxes are not modified and increased water loss is not compensated by drinking. The rapid reduction of Na efflux during transfer from sea water to fresh water is not modified by denervation.

Adaptation, Physiological

Sensitivity of eels (Anguilla anguilla) and carp (Cyprinus carpio) to type C and E botulinum toxin.

This study has shown for the first time that fish such as eels (Anguilla anguilla L.) and carp (Cyprinus carpio L.) are sensitive to type E botulinum toxin. With oral administration the MLD for eels was 27.500 mouse LD50 per kg body weight, while for carp it was only 236 mouse LD50 per kg. Carp were also found to be sensitive to a slight degree to type C botulinum toxin, the MLD of the most virulent toxin of this type being 457,000 mouse LD50 per kg with oral administration. Eels, however, showed no sensitivity to type C toxin.

Administration, Oral

High affinity calcium binding peptides from the gills, gut and kidneys of the freshwater eel (Anguilla anguilla).

We report the isolation of high affinity calcium-binding peptides from homogenates of gills, gut and kidneys of the freshwater eel Anguilla anguilla. These peptides show identical gel-filtration profiles on Biogel P-6, from which a molecular weight of 3500 was estimated. The average binding constant (Kf) for calcium is 7 . 10(7)M-1, and MgCl2 up to 5 mM does not displace calcium.

Animals

[Electroencephalographic activity of a fish (Anguilla anguilla L.) exposed to various hyperbaric conditions (author's transl)].

1. As a continuation of previous work where our methods were fully described (Barthelemy et al., J. Physiol., Paris, 1975, 70, 173-183), the present paper consists of research on EEG activity modifications in the eel (Anguilla anguilla L.) submitted to different hyperbaric conditions (fig. 1). 2. In contrast with mammals, the fish makes it possible to isolate the specific effects of per se pressure: those were researched to a maximum pressure of 101 ATA. Slow and sharp-pointed theta waves appear on EEG tracings of the telencephale, and especially of the tectum opticum (fig. 3), at pressures reaching or beyond 21 ATA, and then they dissappear gradually at higher pressures (101 ATA). The comparison between these effects of per se pressure on the EEG of the fish and the modifications of the EEG of mammals under hyperbaric conditions drives the authors to assume that per se pressure plays a part in the determinism of High Pressure Nervous Syndrome as described for mammals. 3. When submitted to a 21 ATA pressure (below the threshold of per se pressure effects), but when the water and his organism are satured with the N2-O2 mixture (99-1%), the fish shows no EEG modifications to be attributed to inert gas and similar to the EEG symptoms of inert gas narcosis described for mammals when exposed to the same inert gas partial pressures (fig. 5).

Animals

[Blood morphology of the European eel (Anguilla anguilla). II. Studies on granulopoiesis].

It was found that the granulocytes for Anguilla anguilla mainly form in the kidney. The stem cell of granulocytes is the large hemocytoblast. Sequences of development were established for the heterophil, cosinophil and basophil granulocytes. Myeloblasts, promyelocytes, metamyelocytes, mature red-shaped and segmented forms were described as stages of development of the heterophil granulocytes. In the case of the eosinophil and basophil granulocytes the myeloblastic and myelocytic stages could be demonstrated as well as the mature granulocyte. By the aid of special granular staining, phase contrast observations, supravital and cytochemical investigations the granulocytes could be described. The cytochemical proof for the granulocytes delivered a distribution pattern of the lipids, carbohydrates, RNA (RNS) and enzymes: unspecific esterase, alkaline and acid phosphatases, oxydase and peroxydase. By means of heart puncture a larger loss of blood was caused and the hematopoiesis stimulated. The following blood letting gave indications of how rapidly the granulopoiesis develops and how much time maturing takes. After a larger loss of blood the new-growth of the granulocytes will be completed nine days later.

Animals

[Blood morphology of the European eel (Anguilla anguilla). IV. Thrombocytes and their developmental stages].

By the aid of histological special stainings, cytochemical proofs, phase contrast and electron microscopical methods the thrombocytes (spindle cells) of the European ell (Anguilla anguilla) and their stages of development were described as an independent series of cells. After an artificial blood loss thrombocytoblasts, prothrombocytes and mature thrombocytes could distinctly be demonstrates. Besides the kidney the spleen was found to be the main place of the thrombopoiesis. Phase contrast investigations provided evidence for the relatively strong locomotion of these cells. By means of cytochemical proofs a further differentiation of the thrombocytes from other white blood cells can be made. Electron microscopical investigations of the thrombocytes yielded a great similarity to the thrombocytes of the amphibians. The endoplasmatic ring and particular inclusions could be shown to be characteritstic features of the fine structure.

Animals

[Morphological, cytological, and biochemical changes of Anguilla anguilla L. gills deprived of Stannius corpuscles (S. C.) (author's transl)].

In the silver female eel (Anguilla anguilla L.) maintained in fresh water, surgical removal of Stannius corpuscles (SC) resulted in: a) A significant increase of wet weight of gill cells and the dry weight of gill filaments expressed as function of body weight. b) A proliferation and a hypertrophy of chloride cells shown by a significant rise of cell volume expressed as a precentage of the epithelial volume. c) An increase of the calcium (Ca) binding protein activity in the total branchial mucosa. These modifications are not observed when eels are maintained in Ca-free water. The results are discussed in relation to the Ca fluxes across gills in eels deprived of SC and gill morphological changes during sea water adaptation.

Animals

Relative estimation of ventilatory fow rate in the eel (Anguilla anguilla L.)

Freshwater eels (Anguilla anguilla L.) were experimented on in order to measure ventilatory flow rate (by means of a direct method of collecting expired water), and simultaneously, to record sub-opercular pressure changes and opercular displacements (operculogram). Significant correlations (p less than 0.01) were found between the ventilatory flow rate (V) and various characteristics of operculiogram records. The relationships which are significant in each fish experimented on are; V = a(f.lo) + b; V = a(f.Ao) % b and V = a(f.Ao2) + b(f: ventilatory frequency: Ao and lo: mean amplitude and result of integration of opercular tracings). Considering the technical difficulties in measuring the ventilation flow rate of the eel, the easier operculographic recording methods lead to a correct estimation of relative changes in ventilatory flow rate, by using the refined relationship: V = a(Ao1.63).

Animals