Short report concerning trichinellosis in Spain (1977 and 1978). 1. Epidemiological data relating to trichinellosis in man, swine, other domestic animals and wild animals.
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500 adult roes and 100 fawns were examined microscopically for the presence of Sarcocystis gracilis von Ratz, 1901. 95.80 per cent of the roes examined had Sarcosporidias. The parasites were noticed macroscopically in less than a third of them. The abdominal muscles were affected most frequently and most strongly. The leg muscles were affected least of all. Roe bucks were affected to a lesser extent than does. From the third month of life the fawns showed an incidence of parasites which increased with advancing age. During the period of research from May till November hardly any significant difference of parasitical attack occurred in adult roes.
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Pilot trials are described in which BHS, a Czechoslovak fasciolicide, was employed for mass treatment of fasciolosis in cloven-hoofed animals in wild-animals' reserves at the rate of 30 mg body weight using BHS-medicated feed. The treatment was combined with control measures against the intermediate host snail, Lymnaea truncatula, in primary biotopes using Frescon, a molluscicide of English origin, and CF-4, a molluscicide of Czechoslovak origin, at concentration of 1 and 0.5 per cent, respectively. Where the application of molluscicides was impracticable the intermediate host snails were controlled by means of sciomyzid larvae or by blasting surface layers of the soil.--Using this combined approach, the extensity of infestation, evaluated on the basis of coproovoscopical examination, was reduced from 7.05 to 0.71 per cent. The extensity of infestation, evaluated on the basis of necropsy findings, was reduced from 50 to 6.2 per cent. The intensity of infestation was reduced from 7.7 to 0.31 per cent per animal.--The results may prove useful in devising fasciolosis control schemes based on a complex approach.
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A total of 395 wild animals represented by 12 species have been studied over the 1972-1975 period for the presence of sarcosporidia--wild swine, does, hares, jackals, foxes, wild cats, polecats, rats, forest mice, and weasels killed in various regions of the district of Bourgas. Investigated were also several monkeys and wandering dogs. Samples were taken from the muscles of the neck abdomen, hind legs, gut, pharynx, chewing muscles, and hearts to study them for microcysts by the compression method. Sarcosporidia were established in 26.08% of the investigated animals of the following species: wild swine--66.76%, does--71.74%, hares--7.28%, rats--5.50%, and forest mice--10.0%. Most intensive proved parasitizing in does, hares, and swine, and least intensive--in rats and mice. Sarcocysts were found in all investigated viscera, highest being the parasite burden in the heart muscle of a doe. The sarcocyst (microcyst) infection in a particular animal species could be established through investigating the muscles of several organs. All data obtained are given in two tables.
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2238 sera of bovines, 95 of goats, 251 of antelopes (various species), 143 of zebras and 11 of warthogs collected in Tanzania as well as 811 sera of men and females of the city and the region of Dar es Salaam were tested for haemagglutination inhibiting antibodies (Ab) to reovirus serotypes (St) 1, 2 and 3. Ab to St 1 resp. 2 could be detected in 24--39% of bovines, goats, antelopes and zebras, and in warthogs to 64%. In human beings the positive percentage was 60%. Ab to St 3 were most prevalent: Sera of domestic animals were positive in 70--84%, of wild animals in 77--100% and of the human population in 91%. These figures include the occurrence of Ab to one St alone as well as to more than one St. A further analysis showed, that Ab to St 3 are definitely dominant especially in sera of animals, whereas the simultaneous occurrence of Ab to all 3 St was more frequently observed in sera of human beings. Other Ab-type combinations were apparently of far less importance. It seems to be significant that the highest infection rate was found in humans (except zebras and warthogs) and that their contact to animals did not result in a higher Ab conversion rate as without such a possibility. The results were discussed under seroepidemiologic aspects and it is concluded that reovirus infections are facultative viral zoonoses.
Between 1971 and 1974 546 wild animals of 34 species were examined for trypanosomes; 79 infections (9 mixed) were diagnosed and 29 stocks were cryopreserved. Of 14 stocks of the subgenus Trypanozoon tested by the blood incubation infectivity test three (two from Kobus ellipsiprymnus and one from Phacochoerus aethiopicus) retained their infectivity to rodents. There are indications that the number of animals harbouring trypanosomes is far in excess of those showing detectable parasitaemias even when using a combination of diagnostic methods. The standard microhaematocrit method involving microscopic examination of the buffy layer from a microhaematocrit tube between a slide and coverslip, and the inoculation of experimental animals produced the most satisfactory results.
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Sheep were successfully infested artificially with the larvae of Haemonchus contortus, Trichostrongylus axei, Trichostrongylus falculatus and Impalaja nudicollis, cultured from the faeces of blesbok naturally infested with these nematodes. Haemonchus placei, Longistrongylus sabie, Trichostrongylus colubrifomis, T. falculatus, Impalaia tuberculata and Cooperia hungi likewise became established in sheep, goats and calves dosed with larvae cultured from the faeces of infested impala. Sheep and goats could also be infested with Cooperioides hamiltoni and Oesophagostomum columbianum of impala origin, but Cooperioides hepaticae could not be transmitted either to these hosts or to calves.
Ticks were collected over a period of 13 months, at approximately fortnightly intervals, from 6 Africaner oxen kept on the farm Nylsvley in the Naboomspruit District. The 8 species of Ixodidae collected were, in order of abundance, Rhipicephalus appendiculatus, R. evertsi evertsi, Hyalomma marginatum rufipes, H. truncatum, Amblyomma hebraeum, R. simus, Boophilus decoloratus and Ixodes cavipalpus. The relative abundance of adult ticks of these species, their predilection feeding sites and seasonal fluctuations in numbers are discussed.
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