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At least 19 recordsLinked to original sources
PubMed1978

Studies on the subpopulation and function of peripheral lymphocytes, and lymphocyte reactivity to colonic mucosal antigen and bacterial antigen in patients with ulcerative colitis and Crohn's disease.

Immunological studies were carried out in fifteen cases of ulcerative colitis, three cases of tuberculous colitis, four cases of Crohn's disease and one case of Behcet's disease diagnosed by X-ray, endoscopy or biopsy mainly from the standpoint of cellular immunity. In patients with ulcerative colitis the T-cell population and PHA responsiveness of peripheral lymphocytes were both depressed more than in the control group. At different stages of the disease, the deviation of the values of PHA responsiveness of lymphocytes showed a rather wide range in the active stage and there was also immunological instability. However, when the disease entered remission, the immunological conditions settled down and the pathological condition appeared stable. In contrast to the nonspecific immunological conditions mentioned above, characteristically, there were many examples of a positive lymphocyte response to colonic mucosal and bacterial antigens. In all cases of Crohn's disease the lesions are in the small intestine and there was a reduced response to PHA lymphocytic stimulation which was more pronounced than that in patients with ulcerative colitis. PHA response was also high in tuberculous colitis cases.

Adult↗
PubMed1976

Blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates.

Soluble sonicate supernatant preparations were made from Actinomyces viscosus (ATCC 19246), A. naeslundii (ATCC 12104), two strains of Veillonella alcalescens (strain HV-1 and a human oral isolate), Streptococcus sanguis (ATCC 10556), S. mutans (strain 6715-T2), Bacteroides melaninogenicus (strain K110), and Leptotrichia buccalis (isolated from human dental plaque). These supernatants were characterized with reference to their chemical and antigenic components and their biological activity determined by using in vitro lymphocyte blastogenesis as a measure of the host's cellular immune response. The sonicate supernatant of each bacterium contained protein, neutral sugars, methylpentose, and nucleic acids. Protein was the major component in all except L. buccalis, in which neutral sugars predominated. The antigenic components in each supernatant were detected by using rabbit antisera prepared against the whole bacteria and the sonicate supernatant. The supernatants showed a complex antigenic distribution on immunoelectrophoretic analysis. The supernatants were shown to be antigenic and not mitogenic in nature, since neither cord blood lymphocytes nor all adult lymphocytes were stimulated. The supernatant antigen preparations showed a reproducible, dose-dependent, and kinetic response in vitro, which was similar to that seen with the antigen preparation streptokinase-streptodornase.

Actinomyces↗
PubMed1976

Hypersensitivity to bacteria in eczema. I. Bacterial culture, skin tests and immunofluorescent detection of immunoglobulins and bacterial antigens.

A study was made of the cytotoxic effect of antibacterial antibody and complement reacting with bacterial antigens firmly adsorbed to epidermal cells. It is believed that this phenomenon enhances the severity of the lesions and their spread in some cases of disseminated eczema. In this first part of the study it is confirmed that Staphylococcus aureus and micrococci are frequently present on lesions and 'unaffected' skin of patients with disseminated eczema. Intradermal skin tests with antigens of staphylococci and micrococci on 122 eczematous patients elicited immediate, or combined immediate and 4 h (Arthus-like) responses, in a large proportion, but few showed uncombined 4 h responses or delayed hypersensitivity, in contrast to findings reported by others. Immunofluorescence tests on skin of thirty patients showed that IgG and IgM diffused into the epidermis, sometimes to the skin surface, of lesional skin, and more immunoglobulin was found in skin of 'unaffected' areas than in skin of normal healthy persons, indicating that clinically unaffected skin in patients with disseminated eczema is abnormal. IgD was present in three of eight samples of unfixed, and six of eight samples of fixed eczematous skin. Staphylococcal and micrococcal antigen was shown on the skin surface and also diffusely in the cytoplasm of cells in the dermis beneath the surface deposits, indicating percutaneous absorption. Further small amounts of antigen were adsorbed to some epidermal cells. These results show that the predisposing conditions for a cytotoxic reaction mediated by hypersensitivity to bacteria do occur. Increased growth of staphylococci and micrococci on eczematous skin would result in increased deposits of antigen. Bacterial antigens are absorbed into the skin and bind with epidermal cells, and immunoglobulins diffuse into the epidermis. Furthermore, skin tests showed that many eczematous patients were hypersensitive to bacteria. Studies on the nature of the antibacterial antibody will be published in the succeeding reports.

Antigens, Bacterial↗
PubMed1979

[Role of T- and B-lymphocytes in the heterogeneity of human cell-mediated reactions to bacterial antigens].

Leucocytes from 30 patients with allergy to tuberculin and bacterial antigens were treated with antithymus (ATS) and anti-immune globulin (AIGS) sera. The leucocyte migration inhibition test (LMIT) was performed with these antigens. ATS abolished the LMIT induced by tuberculin and sometimes by bacterial antigens (staphylococcal, streptococcal etc.). AIGS frequently abolished the LMIT induced by bacterial antigens, but not by tuberculin. In some cases the treatment with any serum abolished the LMIT induced by the antigens, or, on the contrary, it was abolished only by a successive treatment with both sera. The lymphocyte types (T or B) determining the secondary immune response to the same antigen are different in various patients, as well as they differ in the same patients in relation to diverse antigens. Five types of lymphocyte - antigen interrelation in the LMIT have been distinguished.

Adult↗
PubMed1977

Effect of serum from various animal species on erythrocyte attachment of endotoxins and other bacterial antigens.

Lipopolysaccharide O antigens (endotoxins) and other bacterial antigens readily attach to erythrocytes in vitro. This attachment is prevented by certain mammalian and avian sera. In this study, the inhibitory capacity of sera from lower animals was compared with that of higher animals for a total of 30 species. Antigens and the corresponding antisera included both crude O antigens and purified lipopolysaccharide preparations, the common enterobacterial antigen from Escherichia coli O14, the Vi antigen from Citrobacter ballerup, the polyribose-phosphate antigen from Haemophilus influenzae type b, and the crude teichoic acid antigen from Staphylococcus aureus. Antigen and serum mixtures were incubated at 37 degrees C for 30 min and used for erythrocyte modification; failure of hemagglutination by homologous bacterial antiserum provided evidence of inhibitory capacity. Sera from the classes Mammalia and Aves were very strong inhibitors; those of Reptilia and Osteichthyes were moderate in activity, displaying variation within the classes; those of Amphibia and Chondrichthyes were minimal inhibitors; and those of Merostomata, Crustacea, and Lamellibranchiata displayed questionable or no inhibitory capacity. Inhibitory sera were active with all antigens tested. The findings suggest evolution of inhibitory factors consistent with the theory of two diverging lines of animal phylogeny based on embryological criteria and closely parallel the observations of an endotoxin-altering capacity in vertebrate sera that is not found in invertebrate sera or hemolymph.

Amphibians↗
PubMed1978

Glycoproteins released by Leishmania donovani: immunologic relationships with host and bacterial antigens and preliminary biochemical analysis.

The antigenically active glycoproteins (AAGP) released by Leishmania donovani strain 3S promatigotes into growth media and by amastigotes of this strain into the tissue, e.g. blood, of infected hamsters was found to consist of 6 to 7 antigenically distinct components. The antigenic activity of these glycoproteins was resistant to freeze-thawing, protease treatment, and purification by column chromatography using Sephadex G-100. This activity, however, was destroyed by Na periodate and altered by boiling; AAGP adhered firmly to Amicon filter (UM2). The antigenically active substances absorbed UV at 230, 260, 280 nm and gave positive Folin phenol, phenol sulfuric acid, and orcinol reactions. By gel diffusion, the component glycoproteins were found to form lines with concanavalin A and to give reactions to identity and partial identity with human red cells and Mycobacterium butyricum. The possible involvement of the antigenically active glycoproteins in pathogenesis of kala azar is discussed.

Animals↗
PubMed1976

Blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects.

Cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. Subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. Mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of Actinomyces viscosus (AV), Actinomyces naeslundii (AN), Veillonella alcalescens (VA), Leptotrichia buccalis (LB), Bacteroides melaninogenicus (BM), and homologous dental plaque (DP). The lymphocyte response of subjects with gingivitis or periodontitis was significantly greater than that of normal subjects to antigens of AV, AN, and DP, but did not differ from the response of edentulous subjects. Periodontitis subjects were significantly more reactive than edentulous and normal subjects in response to VA, LB, and BM. These findings suggest that the tested gram-negative bacteria and the host response they evoke are associated with advanced periodontal destruction.

Actinomyces↗
PubMed1979

Comparison of staphylococcal coagglutination, latex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection.

Soluble antigens of Haemophilus influenzae type b, Streptococcus pneumoniae, Neisseria meningitidis, and group B streptococcus were looked for in cerebrospinal fluid, serum, and urine by using the staphylococcal coagglutination test, latex agglutination test, and counterimmunoelectrophoresis. The staphylococcal coaggultination and latex agglutination tests were more sensitive than counterimmunoelectrophoresis in identifying antigens of H. influenzae type b, S. pneumoniae, and N. meningitidis. None of the three tests successfully detected group B streptococcal antigens in body fluids. Nonspecific reactions noted with the staphylococcal coagglutination test could be usually eliminated after premixing test specimens with soluble protein A.

Agglutination Tests↗
PubMed1977

The response of gut-associated lymphoid tissue in gnotobiotic piglets to the presence of bacterial antigen in the alimentary tract.

The cellular changes in the ileum and duodenum and in the mesenteric and prefemoral lymph nodes of gnotobiotic piglets were observed following feeding with a live culture of a non-pathogenic E. coli. There was a rapid and intense reaction in the lower ileum, and follicles were formed; germinal centres were formed in the mesenteric lymph node after a short delay. Germinal centres were not seen in the prefemoral lymph node, though there were pyroninophilic cells in the cortex of this node. Plasma cells were not detected in the medulla of either the mesenteric or the prefemoral lymph nodes, but pyroninophilic cells and plasma cells were found in the lamina propria of the duodenum from 7 days after infection oneards. These observations demonstrate the requirement of an intestinal flora for the development of normal Peyer's patch architecture and indicate that the Peyer's patch response secondarily affects the mesenteric lymph node. The observations also suggest that there is a haematogenous dissemination of pyroninophilic cells from gut-associated lymphoid tissue to, amongst other sites, the duodenum; this may be of importance in both natural and artificial immunization by the oral route.

Animals↗