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[Antistreptolysin-o latex test. A simple screening method for the determination of antistreptolysin-o antibodies].

Experiences with a rapid latex slide test to distinguish elevated fron normal antistreptolysin-O titers are reported. A series of 1000 randomly selected sera were tested in parallel with the ASO latex test and the classical antistreptolysin-O titration test. All sera with an ASO titer of more than 200 I.U. produced a positive agglutination with the ASO latex test. No false negative results were recorded. 6,2% of the sera with a positive ASO latex test exhibited less than 200 I.U. in the titration test. A further advantage of the ASO latex test, apart from its easy practicability, is that it is not inhibited by serum lipids like the hemolytic titration test. The dextransulphate precipitation method combined with the ASO titration showed no advantaged when compared with the ASO latex test combined with the ASO titration without dextransulphate. When patients in our series with ASO TITERS OF 500 AND MORE I.U. were considered in relation to their clinical diagnosis it as found that infections with beta-hemolytic streptococci would not be overlooked, when the ASO latex test is used as a screening procedure. The ASO latex test therefore all s a considerable reduction of personnel and working time. It is however, essential that industry should keep the quality of latex reagents strictly standardized.

Antibodies↗
PubMed1976

Studies on antistreptolysin O activity generated in serum by microorganisms.

An antistreptolysin factor (ASF) was generated in normal human serum by the growth of Staph, aureus and Pseud, aeruginosa. Alpha toxin producing strains of the former were usually positive but activity was not restricted to such strains. Positive strains produce cholesterol esterase which was obtained from DEAE-cellulose column fractions of 18 h broth cultures. Antistreptolysin factor develops slowly in serum, being maximal between the 5th and 10th days and is associated with alterations and disappearance of beta lipoproteins on gel electrophoresis. Activity also appeared in beta lipoproteins precipitated from normal serum with dextran sulphate and redissolved in nutrient broth before inoculation with Staph, aureus. The slow appearance of antistreptolysin activity in serum appears to be due to an esterase inhibitor which is present in high concentrations in some sera. Activity is also modified by the production of a staphylococcal fraction capable of binding to the antistreptolysin factor and reducing its activity. It is suggested that antistreptolysin factor which can be demonstrated in small amounts in normal human serum represents a readily available non-specific defence mechanism capable of binding to certain bacterial products and possible to other foreign protein molecules.

Antistreptolysin↗
PubMed1975

[Normal antistreptolysin titres and demonstration of rheumatoid factors in rhesus monkeys (Macaca mulatta) (author's transl)].

Rhesus monkeys are often used as experimental animals. Many clinical and biochemical parameters are known but there are only few papers dealing with the normal values of antistreptolysin O (ASL). Informations about antistreptococcal antibodies are necessary in order to follow the course of immunization and infection experiments. When non-absorbed sera were used, the mean value of antistreptolysin O in clinically healthy rhesus monkeys (Macacus rhesus) amounted to 178 +/- 52 ASU. After absorption with dextran sulphate, the mean value was 90 +/- 20 ASU. Absorption with dextransulphate is a necessary prerequisite to exact determinations of antistreptolysin titres because it removes unspecific inhibitors of streptolysin O. In the sera of rhesus monkey more inhibitors were present than in human sera. On an average, in monkeys 55% of the material reacting with streptolysin O was an inhibitor, compared with about 40% in human sera. As a result of common infections with streptolysin O-producing streptococcal groups C and G antistreptolysin titres can frequently be seen to increase above the average normal value. Using non-absorbed and dextran sulphate absorbed sera, respectively 350 and 200 ASU must be regarded as the normal upper limits for serum antistreptolysin-titres.

Animals↗
PubMed1977

[The antistreptolysin reaction with and without dextran sulfate addition in various diseases].

It is reported on the results of determinations of the antistreptolysin titre in patients with infectious hepatitis, renal and essential hypertension, mitral stenosis, acute tonsillitis, scarlet fever, rheumatic fever as well as rheumatoid arthritis according to the usual and the dextran sulphate absorption method in altogether 739 patients. Here as in a former publication in 1,700 normal persons partly considerable significant differences between the antistreptolysin titre with and without dextran sulphate were found. The two techniques are discussed, also the influence of the beta-lipoproteins with their proportion of phosphatide on the result of the antistreptolysin titre. It is possible to absorb beta-lipoproteins and perhaps also phosphatides with the help of the addition of dextran sulphate. The present investigations show unequivocally that the absorption the dextran sulphate is a necessary demand which saves the clinician from diagnostic and therapeutic errors. The antistreptolysin reaction is to be regarded only as one constituent for the clinical diagnosis and must not be overvalued in its importance.

Antigen-Antibody Reactions↗
PubMed1977

Two cases of monoclonal immunoglobulins with antistreptolysin activity: clinical and laboratory study.

In two patients with a persisting, high antistreptolysin titre the antistreptolysin activity in both cases resided exclusively in a monoclonal IgG component in the serum. This component had all the characteristics of a true antibody. A history in both patients of arthritis with or without angina suggested that the monoclonal antibodies were reactive in origin, although definite proof was lacking. In one case there was a suggestion of incipient myeloma. Whenever an extremely high antistreptolysin titre persists after antibiotic treatment the possibility of paraproteinaemia should be considered.

Antistreptolysin↗
PubMed1976

[Myelomatous G-kappa immunoglobulin with antistreptolysin O activity].

A new case of myeloma with antistreptolysin O activity is described. The myeloma protein is Kappa IgG. By precipitation of this myeloma IgG with anti-Kappa antiserum, the antistreptolysin O activity is completely inhibited. For the antibody activities of the myeloma protein described in the litterature, the antistreptolysin O activity is the most frequently found. With this 19th case the authors discuss the fundamental problems of the antibody activities of myeloma protein.

Antibody Specificity↗
PubMed1978

New hemolytic method for determination of antistreptolysin O in whole blood.

A new method is proposed for the determination of antistreptolysin O, based on the properties of streptolysin O. The reduced form of the toxin is hemolytic, whereas the oxidized form is not; this activity can be restored, however, by a reducing agent. Both forms maintain the capacity to bind specific antibodies. Unlike the classical method, the assay is performed on whole blood, using the patient's own erythrocytes as revealing agents. A procedure is proposed which limits the operation to a single dilution of a microvolume of blood (0.05 ml brought to 2 ml) and to the distribution of constant quantities in test tubes prefilled with different amounts of oxidized streptolysin O. After a few minutes, a reducing agent is added, and the reading is performed as in the classical technique after sedimentation of the erythrocytes. Correlation with the traditional method (r = 0.987) is evaluated on the basis of the results of samples from hospitalized patients.

Adult↗
PubMed1976

[Titration of antistreptolysin and control of its specificity in respect of percipitation reactions (author's transl)].

A method for the titration of antistreptolysin O (ASLO) is described that is assumed to be more rapid than the methods applied thus far. The authors are drawing attention to the non-specificity of this method and propose to use PRESLO which is easy to prepare and admits of quantitative evaluation, for a control of this non-specificity. Correlation between the two reactions were established by the authors in 2137 sera which was expressed mathematically and made subject to subsequent statistical evaluation.

Antistreptolysin↗
PubMed1977

Comparative study of antistreptolysin O, antideoxyribonuclease B and multienzyme tests in streptococcal infections.

The results of antistreptolysin O (ASO), antideoxyribonuclease B (ADB), and a multienzyme test, Streptozyme, with 162 sera from a stratified random sample of Wisconsin school children, and specimens routinely submitted for serodiagnosis of Group A streptococcal infections are described. A review of the results of Streptozyme tests of laboratories participating in the State Division of Health Proficiency Testing Program, is also presented. The results of these various tests have revealed both false positive and false negative results with the Streptozyme procedure. Although there was good correlation of the Streptozyme findings with the ASO and ADB tests results with sera from young children, false positive Streptozyme test results increased with age of the study subjects. Our results with the ADB test on sera from apparently normal Wisconsin children and young adults suggest that the upper limits of normal ADB values, suggested by the manufacturers of commercial ADB test kits, may be too low.

Adolescent↗
PubMed1975

[Automatic method for determination of serum antistreptolysin].

The AA. describe a method of determination of serum antistreptolysin employing "Technicon" AutoAnalyzer, this method permitting the execution of 40 determinations in an hour, with a time of analysis of about 16 minutes. The procedure of analysis is very easy, with a very high selectivity of values; a variation coefficient of 5,3% and an accuracy index of 4,4% were found. Since the amount of serum employed is 50 mul this method appears to be particularly suitable in paediatry and in serological screening.

Analysis of Variance↗
PubMed1979

[Interest of stimultaneous determination of antistreptolysins and antideoxyribonuclease B in streptococcal infections (author's transl)].

The determination of antistreptolysin O (ASLO) and antideoxyribonuclease B (ADNase B) was carried out on a sample of 199 sera from normal subjects and on a sample of 4019 sera from patients admitted to the Strasbourg University Hospital Center. The sera of hospital origin were divided into two groups. The first group (n = 3889) consisted of sera taken at random, the second group (n = 130) corresponded to patients in whom we isolated a streptococcus of group A. The results obtained show that the distribution of ADNase B and ASLO of normal subjects follow a logarithmic distribution. The geometric averages of the titres DNAase B and ASLO of normal sera and of the first group of hospital origin are scarcely different, whereas the geometrical means of the titres of the second group of sera of hospital origin are quite different and two or three dilutions higher. We noted that the titres of DNAase B and ASLO were in agreement in 84% of cases in the whole group of hospital sera. Among the cases of discrepancy, the negative ASLO and the positive DNAase B corresponded mainly to streptococcal infections.

Antistreptolysin↗