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Structure and evolution of artiodactyla haptoglobins.

1. Artiodactyla haptoglobins (Hps), goat, sheep and cattle (family Bovidae), and pig (family Suidae) were structurally characterized. 2. The polymeric Hp systems of goat, sheep and cattle were similar to the polymeric human Hp system, while the monomeric system of pig was more comparable to the monomeric human form. 3. All members of the Artiodactyla (family Bovidae) examined exhibited a large polypeptide subunit, comparable to that of the beta subunit of human Hp. 4. In addition, a small subunit, similar in molecular weight to the human alpha 2 subunit, was demonstrated. Pig Hp was shown to have two subunits, one slightly larger than the human beta subunit and the other intermediate in size to the human alpha 1 and alpha 2 subunits. 5. Immunoelectrophoretic and immunodiffusion studies indicated complete cross reactivity among the polymeric Artiodactyla Hps. 6. The polymeric Hps do not, however, cross react with the monomeric pig Hp.

Animals

A comparative study of haemoglobins from the Artiodactyla by isoelectric focusing.

1. Isoelectric focusing on 7% acrylamide gels in 2% ampholyte of pH range 6-8 was used to fractionate the haemoglobins of 81 animals, representing 30 species from 4 families of the Order Artiodactyla. 2. Isoelectric points of the major haemoglobins were determined and the relative distribution of minor haemoglobins compared. 3. Marked haemoglobin heterogeneity was observed, with polymorphic and multiple haemoglobins. No one haemoglobin was common to all species.

Animals

[Quantitative characteristics of the systems of truncal auditory and visual brain formations in Artiodactyla and perissodactyla].

On the ground of cytoarchitectonic investigation and planimetric measurements a volumetric comparison between the systems of truncal formations of auditory and optical analysers were made in the representatives of artiodactyla (deer, elk, gazelle, sheep, wild boar, hippopotamus) and perissodactyla orders (horse). The data obtained demonstrated a great quantitative predominance of the system of optical formation in comparison with that of auditory, which correlates to the leading role of the optical system in these animals. In non-ruminants, according to other environmental conditions, vision looses its biological importance, such as in ruminants. In the former, therefore, a much less predominance of the optical formations comparing with the auditory ones was revealed. Comparisons with the previous results of the author obtained in other mammal orders, demonstrated quantative changebility--plasticity of corresponding truncal auditory, optical and vesitbular formations in response to ecologically stipulated changes of leading afferentation in different mammals.

Animals

Observations on the histomorphology of sarcosporidian cysts of some East African game animals (Artiodactyla).

The histomorphology of formalin-fixed micro and macrosarcosporidian cysts of Grant's, Thomson's gazelle, impala, wildebeest, Bubal hartebeest, Cape eland, red duiker, Kirk's dik-dik, defassa waterbuck, Bohor reedbuck, African buffalo, giraffe, warthog, and giant forest hog is described. The wall of microsarcosporidian cysts is smooth and without villi or with villi. The villi are solid or of honeycomb-like structure on tangential sections. In some animals, microsarcosporidian cysts of almost the same diameter with different wall structure were found in the same muscle of the same animal. Some muscle cells contained two or three microsarcosporidian cysts. The wall of macrosarcosporidian cysts of different game species showed also different structure.

Africa, Eastern

[Dermatomycosis of the nilgai antelope (Boselaphus tragocamelus) caused by the dermatophyte trichophyton mentagrophytes].

The authors described a dermal lesion in seven antelopes of the species Boselaphus tragocamelus (Pallas 1776), kept in a zoo-park. Mycological examination revealed the dermatophyte Trichophyton mentagrophytes (Robin) Blanchard 1896 as the causative agent of the lesion. The clinical picture of the dermatophytosis was manifested by a non-inflammatory desquamation of the epidermis with focal hair shedding. The lesions, mostly localized on the heads of the animals affected, were successfully treated with local antimycotics and by oral administration of griseofulvin. The authors present a list of the species of Artiodactyla in which Trichophyton mentagrophytes has been found until the present time. They draw attention to the fact that in wild animals the dermatophyte mostly causes symptomless disease.

Animals

Immunofluorescent localization of acrosin in mammalian spermatozoa (1).

Acrosin was detected by immunofluorescence in the spermatozoan acrosomes of artiodactyla (bull, ram and boar), perissodactyla (horse), carnivora (dog and cat), lagomorpha (rabbit) and primates (human) using anti-bovine acrosin immunoglobulins. The results indicate that the acrosin molecules of several mammalian species possess antigenic similarities.

Acrosin

The phylogenetic distribution of red cell 2,3 diphosphoglycerate and its interaction with mammalian hemoglobins.

In order to better understand the extent to which 2,3 diphosphoglycerate (DPG) contributes to red cell function in the mammals, we assayed DPG levels in blood from a taxonomically diverse set of 71 species representing 14 orders. In addition, for 66 species and 4 hemoglobin phenotypes of the sheep, the effect of DPG on oxygen affinity was measured by determining P 50 values for hemoglobin in the absence of DPG and at 0.2 mM and 1.0 mM concentrations. Most mammals had high levels of red cell DPG and phosphate-free hemoglobins with a relatively high oxygen affinity. In contrast, two taxonomically unrelated groups had both very low intra-erythrocytic DPG concentrations as well as hemoglobins of native low oxygen affinity that interacted weakly with DPG. This latter group includes the Feloidea (order Carnivora) and the Bovoidea (order Artiodactyla). The relationship between DPG concentration, hemoglobin oxygen affinity and the interaction of DPG with hemoglobin is treated quantitatively to provide a model of mammalian red cell function. This derived expression is compared with descriptive allometric equations for whole blood P 50 and is shown to provide statistically reasonable predictions.

Animals

[Ovum lipids of placental mammals].

Morphocytochemical peculiarities of lipids from oocytes of some mammals and man were studied. The human oocytes, according to the structure and content of yolk, bear a close relation to a hare and a rabbit much differing from gametes of predatory animals (dog, cat), artiodactyla (sow), rodents (golden hamster). By thin layer chromatography on silicagel, a detailed lipid composition was established in the rabbit's oocytes, with neutral lipid and phospholipids (kefalin, lecitin, sfingomyelin) dominating. In addition, cholesterin was found.

Adolescent

[Neurophysins of Mammals: evolution and biological signification].

Neurohypophysial hormone-Neurophysin complexes have been prepared from posterior pituitary glands of Artiodactyla (ox, sheep, pig), Perissodactyla (horse) and Cetacea (whale), by fractionated salt precipitation. The components have been separated by molecular sieving in 0.2 M acetic acid and neurophysins have been purified by ion-exchange chromatography on DEAE-Sephadex A-50. Two types of neurophysins, MSEL-neurophysins and VLDV-neurophysins, can be distinguished according to the amino acid residues in positions 2, 3, 6 and 7. MSEL-neurophysins of sheep, ox and pig have been characterized by the amino acid sequence. Ovine and bovine MSEL-neurophysins are nearly identical (one substitution out of 95 residues) and porcine MSEL-neurophysin is very similar (four substitutions and an apparent 3-residue C-terminal deletion). The biological function of neurophysins might be the carriage of neurohypophysial hormones but in this respect, each type of neurophysin is not clearly specific for a given hormone. On the other hand, each neurophysin might share a common precursor with a neurohypophysial hormone, the two parts remaining associated after cleavage. However, in the sheep posterior pituitary gland, the molar proportions of the two types of neurophysins, oxytocin and arginine vasopressin, are not equal, MSEL-neurophysin being more abundant than the other components. If a common precursor exists, neurophysins and neurohypophysial hormones are not merely produced by a simple cleavage mechanism.

Amino Acid Sequence

[Centromere staining in ten species of ruminants by C and R banding techniques].

The centromeric regions of acrocentric or telocentric chromosomes of 10 species of the order Artiodactyla, suborder Ruminantia, have shown to be equally deeply stained either with the C-banding or the R-banding technique. These unusual staining properties could be linked with the presence of a particular type of chromatin. The importance of centromeric fusions linked with speciation in the Bovoidea is recalled.

Animals

[The topography and cytoarchitectonic of the diencephalon of the cow (Bos taurus var. domesticus L.) I. The topography of the diencephalon of the cow. (Bos taurus var. domesticus L.) (author's transl)].

The study contains a comprehensive review of the literature on the brain of the cattle, followed by notes on form and location of the brain in the skull of the cow and of the diencephalon. In addition we point to some possibilities for locating the brain by stereotaxic manipulations. Several structural particularities are discussed, where to a limited extent references to structures of the brains of other domestic artiodactyla are made.

Animals

[Evolutionary aspects of the distribution of Ascaridoidea in mammals (author's transl)].

In an attempt to interpret the distribution of Ascaridoidea in Mammals and Birds, a scheme is proposed, based on the concept of geological periods of evolutionary expansion for parasitic lines. It is assumed that the evolutionary expansion period of Ascaridoidea in Mammals occurred after the diversification of primitive mammals; this would explain the absence of ascarids in these groups. Periods of evolution appear to coincide with the diversification of Carnivora and Sirenia, the first to become invaded; Artiodactyla and Equidae became invaded later, at the time of their diversification. The other cases of parasitism of Mammals by Ascarids are thought to be "captures".

Animals

Distribution of testosterone-estradiol binding globulin (TeBG) in the higher vertebrates.

The presence of testosterone-estradiol binding globulin (TeBG) in a variety of higher vertebrate species has been investigated by an ammonium sulphate precipitation technique. TeBG has been demonstrated in significant amounts in primates, carnivora, marsupiala, and some artiodactyla, even in circumstances where previously electrophoretic studies failed to identify it. For the first time it has also been shown in trace amounts in certain rodents and birds. TeBG in the kangaroo substantiates the primitiveness of the protein in the mammal line and its absence in certain orders and species of eutherian mammals must represent a secondary loss.

Animals

[Anatomy of the lymphangions of the small intestine].

Anatomy of lymphangions (valve segments) has been studied in 120 human and animal cadavers. Methods of investigation were: polychrome injection of arteries, veins and lymphatic bed; macro-microscopic preparation; staining of histopreparations after van Gieson, hematoxylin-eosin, complex technique after A.V. Borisov (1973); impregnation after V.V. Kuprianov (1965); detection of bundle collagene fibers after Mallory and elastic fibers after Weighert. The data collected on the lymphangions demonstrate that the valve segments (lymphangions) in the small intestinal wall appear in phylogenesis at first in birds. Avian lymphangions are cylindric and the valves are of semilunar form and the distance between them is 2--3 cm. In predators (dog, cat, fox, marten) lymphangions are round or conical. Their size is within 6--8 mm. In other animals (rodents, Artiodactyla, Perissodactyla) the lymphangions are 3--5 times longer comparing their width. In human ontogenesis the valve segments of the lymph vessels are formed in tela submucosa of the small intestine in 6--7-month-old fetuses. Some other peculiarities in animal and human anatomy of lymphangions have been revealed.

Animals